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The map label for this gene is rffG [C]

Identifier: 183219540

GI number: 183219540

Start: 116686

End: 117651

Strand: Reverse

Name: rffG [C]

Synonym: LEPBI_I0113

Alternate gene names: 183219540

Gene position: 117651-116686 (Counterclockwise)

Preceding gene: 183219541

Following gene: 183219539

Centisome position: 3.27

GC content: 40.06

Gene sequence:

>966_bases
TTGAAAGTTTTAGTCACTGGTGGTGCAGGTTACATTGGATCCACACTCATCCAATCTTTATTAGAAATCCATCCCGATTG
GTCCATCTTGGCAACGGACATCAAACCGATGAATGTGGAGCTGAAATCTCCTCAGTTTGAATTTCAAATTCTAGATATCA
GTGACCGAAATGCGGTCATCGATTTGATCCAATCATGGAAACCAAATTCCATTGTTCATTTAGCATCCATCCTGAATCCT
CCACCCGGAATGAGTGAAGAAATCCAATGGAAAATTGATGTTCATGGCACCAAAAACGTGTTAGATGGTGCTGTTTATTC
TAAAACGGAACAAGTCATAATCACAAGTTCGGGAGCTGCTTATGGCTATCATAAAGAAAATAAAGAATGGATCGAAGAAA
CAGATCCGATTCGAGGCCACTCTGCCTTTGTTTATTCGAAGCACAAACGAGAAGTAGAGGAATTACTCGCCGAATATAGA
ACCGTTTTCCCAGAGCTCAAACAATTGATCTTACGCCCAGGTACGATTTTAGGAAAAACTGTGAACAACCTAATCACTGA
TATGTTCCAAAAACCGTTTGTGATGGGAATTCTTGGACACAAAAGTCCTTTTGTATTCATTTGGGACGAAGACGTGATTG
CGATCATTGCAAAAGGTATATCCGAAAAGAAAGAAGGAATTTTTAACCTGGCGGGAGATGGAGCACTTAGTTTGAAGGAG
ATCAGTCAGATGATCCGAAAACCTTATGTGCCTATCCCATCATTATTCTTACAATCAATCCTTTGGGTACTTCGGCTCCT
TCGATTGACACAATATGGACCTGACCAAATCGATTTTTTACGTTATAGGCCTGTGTTATCCAACCACCAACTCAAATCCG
TGTTTGGTTACATTCCCAAGTACACTTCCAAAGAAACATTCATTGCTTACTTAAATGCCAAAGGAGTTCCCTATGTTGAA
ATCTAA

Upstream 100 bases:

>100_bases
ATGGCTTTGGTGGCTGCTTTTTGGGGGATTTGGCATATCATTTCTGGATTGATCTTGGTGATGTATTGGAAAAATCACCC
ACCAGTAGAAGGAAATTAAT

Downstream 100 bases:

>100_bases
ATTTGTTTTTGTTTTATTTTTTAGTTATTTTTGCACCTATGCTTCGTTATCTGCCATTCAAGTGGATGTAAAAGATATTA
CGGGTAAAAGTTTGGACCTC

Product: putative UDP-glucose 4-epimerase

Products: UDPgalactose

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKVLVTGGAGYIGSTLIQSLLEIHPDWSILATDIKPMNVELKSPQFEFQILDISDRNAVIDLIQSWKPNSIVHLASILNP
PPGMSEEIQWKIDVHGTKNVLDGAVYSKTEQVIITSSGAAYGYHKENKEWIEETDPIRGHSAFVYSKHKREVEELLAEYR
TVFPELKQLILRPGTILGKTVNNLITDMFQKPFVMGILGHKSPFVFIWDEDVIAIIAKGISEKKEGIFNLAGDGALSLKE
ISQMIRKPYVPIPSLFLQSILWVLRLLRLTQYGPDQIDFLRYRPVLSNHQLKSVFGYIPKYTSKETFIAYLNAKGVPYVE
I

Sequences:

>Translated_321_residues
MKVLVTGGAGYIGSTLIQSLLEIHPDWSILATDIKPMNVELKSPQFEFQILDISDRNAVIDLIQSWKPNSIVHLASILNP
PPGMSEEIQWKIDVHGTKNVLDGAVYSKTEQVIITSSGAAYGYHKENKEWIEETDPIRGHSAFVYSKHKREVEELLAEYR
TVFPELKQLILRPGTILGKTVNNLITDMFQKPFVMGILGHKSPFVFIWDEDVIAIIAKGISEKKEGIFNLAGDGALSLKE
ISQMIRKPYVPIPSLFLQSILWVLRLLRLTQYGPDQIDFLRYRPVLSNHQLKSVFGYIPKYTSKETFIAYLNAKGVPYVE
I
>Mature_321_residues
MKVLVTGGAGYIGSTLIQSLLEIHPDWSILATDIKPMNVELKSPQFEFQILDISDRNAVIDLIQSWKPNSIVHLASILNP
PPGMSEEIQWKIDVHGTKNVLDGAVYSKTEQVIITSSGAAYGYHKENKEWIEETDPIRGHSAFVYSKHKREVEELLAEYR
TVFPELKQLILRPGTILGKTVNNLITDMFQKPFVMGILGHKSPFVFIWDEDVIAIIAKGISEKKEGIFNLAGDGALSLKE
ISQMIRKPYVPIPSLFLQSILWVLRLLRLTQYGPDQIDFLRYRPVLSNHQLKSVFGYIPKYTSKETFIAYLNAKGVPYVE
I

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Saccharomyces cerevisiae, GI6321437, Length=195, Percent_Identity=28.7179487179487, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2

Molecular weight: Translated: 36340; Mature: 36340

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVTGGAGYIGSTLIQSLLEIHPDWSILATDIKPMNVELKSPQFEFQILDISDRNAVI
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEEEEEECCCHHHH
DLIQSWKPNSIVHLASILNPPPGMSEEIQWKIDVHGTKNVLDGAVYSKTEQVIITSSGAA
HHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHCCHHHCCCCEEEEEECCCC
YGYHKENKEWIEETDPIRGHSAFVYSKHKREVEELLAEYRTVFPELKQLILRPGTILGKT
CCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VNNLITDMFQKPFVMGILGHKSPFVFIWDEDVIAIIAKGISEKKEGIFNLAGDGALSLKE
HHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCHHH
ISQMIRKPYVPIPSLFLQSILWVLRLLRLTQYGPDQIDFLRYRPVLSNHQLKSVFGYIPK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHHCCC
YTSKETFIAYLNAKGVPYVEI
CCCCCEEEEEEECCCCCEEEC
>Mature Secondary Structure
MKVLVTGGAGYIGSTLIQSLLEIHPDWSILATDIKPMNVELKSPQFEFQILDISDRNAVI
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEEEEEECCCHHHH
DLIQSWKPNSIVHLASILNPPPGMSEEIQWKIDVHGTKNVLDGAVYSKTEQVIITSSGAA
HHHHCCCCCCHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHCCHHHCCCCEEEEEECCCC
YGYHKENKEWIEETDPIRGHSAFVYSKHKREVEELLAEYRTVFPELKQLILRPGTILGKT
CCCCCCCHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
VNNLITDMFQKPFVMGILGHKSPFVFIWDEDVIAIIAKGISEKKEGIFNLAGDGALSLKE
HHHHHHHHHHCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCEEECCCCCCCHHH
ISQMIRKPYVPIPSLFLQSILWVLRLLRLTQYGPDQIDFLRYRPVLSNHQLKSVFGYIPK
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCHHHHHHHHCCC
YTSKETFIAYLNAKGVPYVEI
CCCCCEEEEEEECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose

Specific reaction: UDP-glucose = UDP-galactose

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]