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The map label for this gene is kefB [H]

Identifier: 183219512

GI number: 183219512

Start: 84255

End: 86363

Strand: Reverse

Name: kefB [H]

Synonym: LEPBI_I0085

Alternate gene names: 183219512

Gene position: 86363-84255 (Counterclockwise)

Preceding gene: 183219513

Following gene: 183219511

Centisome position: 2.4

GC content: 41.16

Gene sequence:

>2109_bases
ATGAAAACTCGTTCTACTGTTTTTTACGGTTTTACACTATTATTGTTTGGATCCTTAGGATATTACTTACTCCAAGCAGG
TGGTGTTTTAGAATCTACGAAAAACCTTACCATCGCAACGAACGGTCACTTAGATACAGAAAATTTTTTCAATCGGTTCC
ACCATCCTTTAGCTTTATTATTTTTACAGATCATCGTGGTATGTGGATCAGCTCGGTTTGTTGGTTATCTATTCACAAGA
AAACTCAAACAACCATCTGTGATGGGAGAAATTGTAGCGGGGATCTTACTTGGACCGTCCCTACTCGGTTATTATTTTCC
AGAGACAATGAGTTTCCTCTTCCCTCCTTCAAGTCTTCCTACACTCGGAACATTAAGCCAAATCGGTCTTGTTTTATTTA
TGTTCATCATCGGGATGGAACTTGATCTTTCTGTTCTGAAAAACAAAGCCCATTCTGCAATTATCATCAGTCATGCGAGT
ATCATCTTTCCTTTCTTTTTGGGAATGACTTTGGCTTATTATTTTTACACAGACTATGCTCCCGAGAATGTAGGTTTTTT
ATCATTCTCTTTATTTATGGGAATTGCGATGAGTATCACTGCTTTTCCGGTGCTTGCACGAATCTTACAAGAACGGAATC
TCACACGTACTCCGCTCGGTGCAATGGTTCTCACATGTGCCGCAGCCGATGATATCACAGCTTGGATCCTTCTTGCGATT
ATTGTTACCATTTCCAAAGCAGGAAACTTAAACACTGCCTTATTTACGATCGGACTTTCGTTTGCGTACATCCTTACTAT
GATTTATTTGGTGGCCCCGTTTCTCAAACGATTGGGTTCCATTTACATTTCTCGTGAGAATCTCACAAGGACAGCTGTTG
CGCTCATCTTAATGATTCTATTCTTATCATCTCTCACTACAGAAGTGATTGGAATCCATGCCCTGTTTGGAGCTTTCCTT
GCCGGTGTCATTATGCCTTCTGAAGGAAATCTTAAAAAACTCATCGCGGAAAAAATCGAAGACATCGCGGTCATTTTGTT
CCTTCCGATTTTCTTTGTCATCACTGGCCTTAGGACAGAAGTGACTCTCCTCAATGGCTCTCATCTTTGGTTGGTGTTTG
GACTGGTTCTCTTTGTGGCAGTCGTCGGTAAGTTTTTAGGAAGTGCTTTGGCCGCAAGAGTCTCTGGGTCCAATTGGGAA
GACTCTTTATCGATTGGTGCCCTCATGAATACACGAGGCCTTATGGAACTCGTTGTCCTCAATATTGGTTATGATTTAGG
AATCCTAAGTCCAGAAATTTTTGCAGTCTTTGTCCTCATGGCACTCGTGACCACTCTTTCCACGGGACCTCTCCTTGATG
GGATCCAAAAGTTTTTTGCGAGAACAGCAAATGCAACATATCCAGAAAAACCATCCGACAGCAAACTCCGAGTCCTTGTT
GCTTTTGCCCAAGAGAAAATGGGAAAAAGTTTGGTTCGTTTTGCCTTCTCTTTATCCGGTAACCAAAAGAAAAATTTAGA
ACTCATTGCATTACACATTTCGCCTAACGACTCATTGTCCAACGAAGAAATCAGAAAGTATCGGGATGCCAGTTTCGAAG
CGATCCGCCAAACAGGATCCAGCTTAGGTATCCAAGTCCAAACCGAATACCGCATCACAGACAATGTCACATATGAAATT
GTGAATTTTGCCAAAATCAAACATACGGACATCCTTCTCATTGGAGCTGCAAAACCACTTTTTTCTCGTAGTTATACTGG
CGGAAAAATCAAAGGGATTCTCAATTATTGCCCGGCAACAGTAGGTGTCCTCATTGACAATGGCCTAGAATCCATTGAAA
GAGTTGCCATTCTTTACAAAGGGGAAAAAGACCCCATCCTAGGTTTTGCACAAAAACTCACCTCCTTAAAGGGAATGAAA
TTTAACAAAATCAAAGTGGAAAACCTCATCCAACCTGAAACAGATCTCAATCCATTCCCAATTGCCTTAAGCCAAATCAC
AGGGTATTCACTCATTCTCATCGATCTCAATGTTTGGGAAGAATTGGGTTTTGAAAAAATGGATCTTCTCCCCACCTCTT
TTCTTTTGGTCCGATTTTTAACCACCTAA

Upstream 100 bases:

>100_bases
AAGTAGCAAGGCATGGTAGAGCTGTATCCAATCAAGTCCCTGATGTTGTAGGGGAAGCCCTTGTGAAAAAATTCTCCGTT
TGATCGGACTTTGAGGATCT

Downstream 100 bases:

>100_bases
AGATTCGATTGCTTTTTTCGGTTTCTCCAAGCAACTGGAGATATGGAAAGAACTTTTATCATGCTTAAACCCGATGCAGT
GAAAAACAAACACATCGGTG

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MKTRSTVFYGFTLLLFGSLGYYLLQAGGVLESTKNLTIATNGHLDTENFFNRFHHPLALLFLQIIVVCGSARFVGYLFTR
KLKQPSVMGEIVAGILLGPSLLGYYFPETMSFLFPPSSLPTLGTLSQIGLVLFMFIIGMELDLSVLKNKAHSAIIISHAS
IIFPFFLGMTLAYYFYTDYAPENVGFLSFSLFMGIAMSITAFPVLARILQERNLTRTPLGAMVLTCAAADDITAWILLAI
IVTISKAGNLNTALFTIGLSFAYILTMIYLVAPFLKRLGSIYISRENLTRTAVALILMILFLSSLTTEVIGIHALFGAFL
AGVIMPSEGNLKKLIAEKIEDIAVILFLPIFFVITGLRTEVTLLNGSHLWLVFGLVLFVAVVGKFLGSALAARVSGSNWE
DSLSIGALMNTRGLMELVVLNIGYDLGILSPEIFAVFVLMALVTTLSTGPLLDGIQKFFARTANATYPEKPSDSKLRVLV
AFAQEKMGKSLVRFAFSLSGNQKKNLELIALHISPNDSLSNEEIRKYRDASFEAIRQTGSSLGIQVQTEYRITDNVTYEI
VNFAKIKHTDILLIGAAKPLFSRSYTGGKIKGILNYCPATVGVLIDNGLESIERVAILYKGEKDPILGFAQKLTSLKGMK
FNKIKVENLIQPETDLNPFPIALSQITGYSLILIDLNVWEELGFEKMDLLPTSFLLVRFLTT

Sequences:

>Translated_702_residues
MKTRSTVFYGFTLLLFGSLGYYLLQAGGVLESTKNLTIATNGHLDTENFFNRFHHPLALLFLQIIVVCGSARFVGYLFTR
KLKQPSVMGEIVAGILLGPSLLGYYFPETMSFLFPPSSLPTLGTLSQIGLVLFMFIIGMELDLSVLKNKAHSAIIISHAS
IIFPFFLGMTLAYYFYTDYAPENVGFLSFSLFMGIAMSITAFPVLARILQERNLTRTPLGAMVLTCAAADDITAWILLAI
IVTISKAGNLNTALFTIGLSFAYILTMIYLVAPFLKRLGSIYISRENLTRTAVALILMILFLSSLTTEVIGIHALFGAFL
AGVIMPSEGNLKKLIAEKIEDIAVILFLPIFFVITGLRTEVTLLNGSHLWLVFGLVLFVAVVGKFLGSALAARVSGSNWE
DSLSIGALMNTRGLMELVVLNIGYDLGILSPEIFAVFVLMALVTTLSTGPLLDGIQKFFARTANATYPEKPSDSKLRVLV
AFAQEKMGKSLVRFAFSLSGNQKKNLELIALHISPNDSLSNEEIRKYRDASFEAIRQTGSSLGIQVQTEYRITDNVTYEI
VNFAKIKHTDILLIGAAKPLFSRSYTGGKIKGILNYCPATVGVLIDNGLESIERVAILYKGEKDPILGFAQKLTSLKGMK
FNKIKVENLIQPETDLNPFPIALSQITGYSLILIDLNVWEELGFEKMDLLPTSFLLVRFLTT
>Mature_702_residues
MKTRSTVFYGFTLLLFGSLGYYLLQAGGVLESTKNLTIATNGHLDTENFFNRFHHPLALLFLQIIVVCGSARFVGYLFTR
KLKQPSVMGEIVAGILLGPSLLGYYFPETMSFLFPPSSLPTLGTLSQIGLVLFMFIIGMELDLSVLKNKAHSAIIISHAS
IIFPFFLGMTLAYYFYTDYAPENVGFLSFSLFMGIAMSITAFPVLARILQERNLTRTPLGAMVLTCAAADDITAWILLAI
IVTISKAGNLNTALFTIGLSFAYILTMIYLVAPFLKRLGSIYISRENLTRTAVALILMILFLSSLTTEVIGIHALFGAFL
AGVIMPSEGNLKKLIAEKIEDIAVILFLPIFFVITGLRTEVTLLNGSHLWLVFGLVLFVAVVGKFLGSALAARVSGSNWE
DSLSIGALMNTRGLMELVVLNIGYDLGILSPEIFAVFVLMALVTTLSTGPLLDGIQKFFARTANATYPEKPSDSKLRVLV
AFAQEKMGKSLVRFAFSLSGNQKKNLELIALHISPNDSLSNEEIRKYRDASFEAIRQTGSSLGIQVQTEYRITDNVTYEI
VNFAKIKHTDILLIGAAKPLFSRSYTGGKIKGILNYCPATVGVLIDNGLESIERVAILYKGEKDPILGFAQKLTSLKGMK
FNKIKVENLIQPETDLNPFPIALSQITGYSLILIDLNVWEELGFEKMDLLPTSFLLVRFLTT

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=301, Percent_Identity=28.2392026578073, Blast_Score=63, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322367, Length=437, Percent_Identity=33.6384439359268, Blast_Score=251, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 77222; Mature: 77222

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRSTVFYGFTLLLFGSLGYYLLQAGGVLESTKNLTIATNGHLDTENFFNRFHHPLALL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
FLQIIVVCGSARFVGYLFTRKLKQPSVMGEIVAGILLGPSLLGYYFPETMSFLFPPSSLP
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TLGTLSQIGLVLFMFIIGMELDLSVLKNKAHSAIIISHASIIFPFFLGMTLAYYFYTDYA
CHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
PENVGFLSFSLFMGIAMSITAFPVLARILQERNLTRTPLGAMVLTCAAADDITAWILLAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
IVTISKAGNLNTALFTIGLSFAYILTMIYLVAPFLKRLGSIYISRENLTRTAVALILMIL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHH
FLSSLTTEVIGIHALFGAFLAGVIMPSEGNLKKLIAEKIEDIAVILFLPIFFVITGLRTE
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VTLLNGSHLWLVFGLVLFVAVVGKFLGSALAARVSGSNWEDSLSIGALMNTRGLMELVVL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
NIGYDLGILSPEIFAVFVLMALVTTLSTGPLLDGIQKFFARTANATYPEKPSDSKLRVLV
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
AFAQEKMGKSLVRFAFSLSGNQKKNLELIALHISPNDSLSNEEIRKYRDASFEAIRQTGS
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SLGIQVQTEYRITDNVTYEIVNFAKIKHTDILLIGAAKPLFSRSYTGGKIKGILNYCPAT
CCCEEEEEEEEEECCCCEEEHHHHEEECCCEEEEECCCHHHHCCCCCCHHHHHHHHCCHH
VGVLIDNGLESIERVAILYKGEKDPILGFAQKLTSLKGMKFNKIKVENLIQPETDLNPFP
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCCC
IALSQITGYSLILIDLNVWEELGFEKMDLLPTSFLLVRFLTT
HHHHHCCCCEEEEEEEEHHHHCCCCHHHHCHHHHHHHHHHCC
>Mature Secondary Structure
MKTRSTVFYGFTLLLFGSLGYYLLQAGGVLESTKNLTIATNGHLDTENFFNRFHHPLALL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHH
FLQIIVVCGSARFVGYLFTRKLKQPSVMGEIVAGILLGPSLLGYYFPETMSFLFPPSSLP
HHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TLGTLSQIGLVLFMFIIGMELDLSVLKNKAHSAIIISHASIIFPFFLGMTLAYYFYTDYA
CHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCC
PENVGFLSFSLFMGIAMSITAFPVLARILQERNLTRTPLGAMVLTCAAADDITAWILLAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
IVTISKAGNLNTALFTIGLSFAYILTMIYLVAPFLKRLGSIYISRENLTRTAVALILMIL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHH
FLSSLTTEVIGIHALFGAFLAGVIMPSEGNLKKLIAEKIEDIAVILFLPIFFVITGLRTE
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VTLLNGSHLWLVFGLVLFVAVVGKFLGSALAARVSGSNWEDSLSIGALMNTRGLMELVVL
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
NIGYDLGILSPEIFAVFVLMALVTTLSTGPLLDGIQKFFARTANATYPEKPSDSKLRVLV
HCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
AFAQEKMGKSLVRFAFSLSGNQKKNLELIALHISPNDSLSNEEIRKYRDASFEAIRQTGS
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCC
SLGIQVQTEYRITDNVTYEIVNFAKIKHTDILLIGAAKPLFSRSYTGGKIKGILNYCPAT
CCCEEEEEEEEEECCCCEEEHHHHEEECCCEEEEECCCHHHHCCCCCCHHHHHHHHCCHH
VGVLIDNGLESIERVAILYKGEKDPILGFAQKLTSLKGMKFNKIKVENLIQPETDLNPFP
HHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCCC
IALSQITGYSLILIDLNVWEELGFEKMDLLPTSFLLVRFLTT
HHHHHCCCCEEEEEEEEHHHHCCCCHHHHCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]