Click here to switch to the map view.

The map label for this gene is gmuD [H]

Identifier: 18311291

GI number: 18311291

Start: 2654377

End: 2655756

Strand: Reverse

Name: gmuD [H]

Synonym: CPE2309

Alternate gene names: 18311291

Gene position: 2655756-2654377 (Counterclockwise)

Preceding gene: 18311292

Following gene: 18311290

Centisome position: 87.61

GC content: 31.67

Gene sequence:

>1380_bases
ATGAAATATACTTTCCCAGAAAACTTTTGGTGGGGAGCTGCAACTTCAGGACCTCAGTCAGAAGGTAGGTTTAATAAAAA
ACATGATAATGTATTTGATCACTGGTTCGATATAAATCCAGAGTTATTTCACAATGGTATAGGACCAAATATAGCATCAA
ATTTTTATAATAGTTACAAAGAAGATTTAGCAATGCTAAAAGAAATTGGATTAAATTCATTTAGAACTTCAATTCAATGG
ACAAGGTTAATTAAAGATTTTGAAACTGGTGAGCCAGATGAAGATGGAGTAAGATTTTATAATGGTGTAATAGATGAATG
TTTAGCAAATGGAATAGATATAATCATGAATTTACATCACTTTGATCTTCCTGTTGAATTATATGATAAATATGGTGGAT
GGGAATCAAAGCATGTCGTAGAATTATTTGCGAAATTTGCTAAGACTGCCTTTAGTTTATTTGGTGATAGGGTTAAAAAA
TGGGCTACATTTAATGAACCTATAGTTATTATTGAAGGACAGTTTTTATATAAATGGCATTATCCTTGTATAGTTGATGG
AAAAAGAGGGCTTCAAGCTGCCTATAATATAGCATTAGCTTCTGCTAGAGCCATAGAAGAGTATAGAAAATTAGGGCAAG
ATGGAGAAATAGGAATAATAGTTAACTTAACGCCAGCATATCCAAGAAGTGAGTCAAAAGAAGATTTAAGAGCGGCTGAA
ATTGCCAACGCTTTCTTCAATGAGTTATTCTTAGATCCAGCAACTAAGGGAGAATTTCCTAAGAAATTAGTTGAGGTTTT
AGAAAAAGATGGAGTAATGTGGAATTCTACTAAGGAAGAATTAGAGGTTATAAAAAATAATACTGTGGATTTCTTAGGGG
TAAACTACTATCAACCAAGAAGAGTTAAAGATAGAGAAGAGGAATATGGTGGAGAAACATGGGCTCCAGAAAAATATTTT
GATAATTATGATATGCCAGGAAAGAGAATGAATCCCCATAGAGGATGGGAAATATACCCTAAGGCAATTTATGATATAGC
TAAAAATGTTCAAGAGAACTATGGCAACATAAAATGGTTCATTTCAGAAAATGGAATGGGTGTTGAAGGAGAAGAAAAAT
TCAAAAATGCTGAAGGTATAATTGAAGATGATTATAGGATTGAATTCATAATAGAGCATTTAGAATGGCTTCATAAGGCT
ATTGAAGAGGGTTCAAATTGTGTGGGATATCATTTATGGACTCCAATAGATTGTTGGTCATGGTTAAATTCATATAAGAA
TAGATATGGATTTATATCATTAGATTTAGAAACTCAAAAGAAAACTATTAAAAAATCAGGAAGATGGATAAAAGAAGTTT
CTAAGAATAATGGTTTTTAA

Upstream 100 bases:

>100_bases
TTTTTTTGGAATTAAGCCACATAAGAAACCTAGAAAAAAGTTTTTTTAAAATATACAATTAAGTAGAGAAAAAGTTTACA
AAGGTATAGGGGGTTTTCAC

Downstream 100 bases:

>100_bases
AACTTTGAAGTTACTTAGCTTAGATTAATTCTAAAATAAATTTTCTAAGTTAATATGACTACGCAAAAAGTTTTTTATTA
TAAAATTATAAATAAAATAA

Product: beta-glucosidase

Products: NA

Alternate protein names: Aryl-phospho-beta-D-glucosidase BglD; Glucomannan utilization protein D [H]

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKYTFPENFWWGAATSGPQSEGRFNKKHDNVFDHWFDINPELFHNGIGPNIASNFYNSYKEDLAMLKEIGLNSFRTSIQW
TRLIKDFETGEPDEDGVRFYNGVIDECLANGIDIIMNLHHFDLPVELYDKYGGWESKHVVELFAKFAKTAFSLFGDRVKK
WATFNEPIVIIEGQFLYKWHYPCIVDGKRGLQAAYNIALASARAIEEYRKLGQDGEIGIIVNLTPAYPRSESKEDLRAAE
IANAFFNELFLDPATKGEFPKKLVEVLEKDGVMWNSTKEELEVIKNNTVDFLGVNYYQPRRVKDREEEYGGETWAPEKYF
DNYDMPGKRMNPHRGWEIYPKAIYDIAKNVQENYGNIKWFISENGMGVEGEEKFKNAEGIIEDDYRIEFIIEHLEWLHKA
IEEGSNCVGYHLWTPIDCWSWLNSYKNRYGFISLDLETQKKTIKKSGRWIKEVSKNNGF

Sequences:

>Translated_459_residues
MKYTFPENFWWGAATSGPQSEGRFNKKHDNVFDHWFDINPELFHNGIGPNIASNFYNSYKEDLAMLKEIGLNSFRTSIQW
TRLIKDFETGEPDEDGVRFYNGVIDECLANGIDIIMNLHHFDLPVELYDKYGGWESKHVVELFAKFAKTAFSLFGDRVKK
WATFNEPIVIIEGQFLYKWHYPCIVDGKRGLQAAYNIALASARAIEEYRKLGQDGEIGIIVNLTPAYPRSESKEDLRAAE
IANAFFNELFLDPATKGEFPKKLVEVLEKDGVMWNSTKEELEVIKNNTVDFLGVNYYQPRRVKDREEEYGGETWAPEKYF
DNYDMPGKRMNPHRGWEIYPKAIYDIAKNVQENYGNIKWFISENGMGVEGEEKFKNAEGIIEDDYRIEFIIEHLEWLHKA
IEEGSNCVGYHLWTPIDCWSWLNSYKNRYGFISLDLETQKKTIKKSGRWIKEVSKNNGF
>Mature_459_residues
MKYTFPENFWWGAATSGPQSEGRFNKKHDNVFDHWFDINPELFHNGIGPNIASNFYNSYKEDLAMLKEIGLNSFRTSIQW
TRLIKDFETGEPDEDGVRFYNGVIDECLANGIDIIMNLHHFDLPVELYDKYGGWESKHVVELFAKFAKTAFSLFGDRVKK
WATFNEPIVIIEGQFLYKWHYPCIVDGKRGLQAAYNIALASARAIEEYRKLGQDGEIGIIVNLTPAYPRSESKEDLRAAE
IANAFFNELFLDPATKGEFPKKLVEVLEKDGVMWNSTKEELEVIKNNTVDFLGVNYYQPRRVKDREEEYGGETWAPEKYF
DNYDMPGKRMNPHRGWEIYPKAIYDIAKNVQENYGNIKWFISENGMGVEGEEKFKNAEGIIEDDYRIEFIIEHLEWLHKA
IEEGSNCVGYHLWTPIDCWSWLNSYKNRYGFISLDLETQKKTIKKSGRWIKEVSKNNGF

Specific function: Phospho-beta-D-glucosidase that seems to be involved in the degradation of glucomannan. Is also capable of hydrolyzing aryl-phospho-beta-D-glucosides, although very weakly, and plays only a minor role, if any, in the degradation of these substrates in viv

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI13273313, Length=479, Percent_Identity=31.3152400835073, Blast_Score=192, Evalue=7e-49,
Organism=Homo sapiens, GI110681710, Length=486, Percent_Identity=28.1893004115226, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI32481206, Length=485, Percent_Identity=28.2474226804124, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI24497614, Length=482, Percent_Identity=24.896265560166, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28376633, Length=475, Percent_Identity=25.4736842105263, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI2367174, Length=492, Percent_Identity=30.4878048780488, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1789070, Length=480, Percent_Identity=31.0416666666667, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI2367270, Length=487, Percent_Identity=29.1581108829569, Blast_Score=191, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17539390, Length=480, Percent_Identity=33.75, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17552856, Length=472, Percent_Identity=31.3559322033898, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI21356577, Length=481, Percent_Identity=26.6112266112266, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53503; Mature: 53503

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYTFPENFWWGAATSGPQSEGRFNKKHDNVFDHWFDINPELFHNGIGPNIASNFYNSYK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
EDLAMLKEIGLNSFRTSIQWTRLIKDFETGEPDEDGVRFYNGVIDECLANGIDIIMNLHH
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHEEHHH
FDLPVELYDKYGGWESKHVVELFAKFAKTAFSLFGDRVKKWATFNEPIVIIEGQFLYKWH
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEC
YPCIVDGKRGLQAAYNIALASARAIEEYRKLGQDGEIGIIVNLTPAYPRSESKEDLRAAE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
IANAFFNELFLDPATKGEFPKKLVEVLEKDGVMWNSTKEELEVIKNNTVDFLGVNYYQPR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCCCCC
RVKDREEEYGGETWAPEKYFDNYDMPGKRMNPHRGWEIYPKAIYDIAKNVQENYGNIKWF
CCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCEEEE
ISENGMGVEGEEKFKNAEGIIEDDYRIEFIIEHLEWLHKAIEEGSNCVGYHLWTPIDCWS
EECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
WLNSYKNRYGFISLDLETQKKTIKKSGRWIKEVSKNNGF
HHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYTFPENFWWGAATSGPQSEGRFNKKHDNVFDHWFDINPELFHNGIGPNIASNFYNSYK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHH
EDLAMLKEIGLNSFRTSIQWTRLIKDFETGEPDEDGVRFYNGVIDECLANGIDIIMNLHH
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHEEHHH
FDLPVELYDKYGGWESKHVVELFAKFAKTAFSLFGDRVKKWATFNEPIVIIEGQFLYKWH
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEC
YPCIVDGKRGLQAAYNIALASARAIEEYRKLGQDGEIGIIVNLTPAYPRSESKEDLRAAE
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHH
IANAFFNELFLDPATKGEFPKKLVEVLEKDGVMWNSTKEELEVIKNNTVDFLGVNYYQPR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEECCCHHHHHHHHCCCEEEEECCCCCCC
RVKDREEEYGGETWAPEKYFDNYDMPGKRMNPHRGWEIYPKAIYDIAKNVQENYGNIKWF
CCCCHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCCEECHHHHHHHHHHHHHCCCCEEEE
ISENGMGVEGEEKFKNAEGIIEDDYRIEFIIEHLEWLHKAIEEGSNCVGYHLWTPIDCWS
EECCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHH
WLNSYKNRYGFISLDLETQKKTIKKSGRWIKEVSKNNGF
HHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]