BacMap

Definition	Clostridium perfringens str. 13, complete genome.
Accession	NC_003366
Length	3,031,430

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The map label for this gene is crt [H]

Identifier: 18311283

GI number: 18311283

Start: 2645717

End: 2646499

Strand: Reverse

Name: crt [H]

Synonym: CPE2301

Alternate gene names: 18311283

Gene position: 2646499-2645717 (Counterclockwise)

Preceding gene: 18311284

Following gene: 18311282

Centisome position: 87.3

GC content: 32.69

Gene sequence:

>783_bases
ATGGAATTAAATAATGTAATCTTTGAAAAAGAGGGAAACATTGGGGTTTTAACAATTAACAGACCTAAGGCATTAAATGC
ACTAAACTCAGAAACTTTAAAAGACTTAGATACTGCTATAGATCATATAGAAAAACAAGATGATATATATGTTGTTATTT
TAACAGGTGCTGGAGATAAAGCATTTGTTGCAGGTGCTGATATAGCAGAAATGAAAGATCTAAATGAAGAAGAGGGTAAA
GAATTTGGACTTTTAGGTAATAAAGTGTTTAGAAGATTAGAAAATTTAGACAAGCCTGTAATCGCTGCTATAAACGGTTT
CGCTTTAGGTGGTGGATGTGAAATTTCTATGGCTTGTGATATAAGAATAGCAACTACAAAGGCTAAGTTTGCTCAACCTG
AGGTTGGATTAGGAATAACTCCAGGATTTGGTGGAACTCAAAGATTACCAAGAATAGTTGGACCTGGTAAGGCTAAAGAA
CTTATTTACACAGGAGACATGATAAAAGCTGATGAAGCTTTAAGAATTGGTTTAGTAAATAAGGTTGTTGAACCAGAGAA
TTTAATGGAAGAAGCAATGTCATTAGCTAAGAAAATATCTAATAATGCACCAATAGCTGTTAAGTTATGTAAAGATGCAA
TCAATAGAGGAATTCAAGTTGATATAGATTCAGCTGTAGTTATAGAAGCTGAAGATTTTGGAAAATGTTTTGCTACAGAA
GATCAAACAGAAGGTATGAGTGCTTTTGTAGAGAGAAGAGAAAAGAACTTTAAAAACAAGTAA

Upstream 100 bases:

>100_bases
CTGGAAAAAGCATAGGATAAAAAATATTAAGTTGTTAGTTATTAGTTGTTAAGTTATTTAAAACCATATATTCGCTATGG
TATATGAGGGAGGAAATACA

Downstream 100 bases:

>100_bases
TTTTTTAAACAAAATGGTTGTGTGGATTTAAGGAGGGTAATTAAAAATGAACTTTCAATTAACAAGAGAACAAGAATTAG
TTAAGCAAATGGTTAGAGAA

Product: 3-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MELNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAFVAGADIAEMKDLNEEEGK
EFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACDIRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKE
LIYTGDMIKADEALRIGLVNKVVEPENLMEEAMSLAKKISNNAPIAVKLCKDAINRGIQVDIDSAVVIEAEDFGKCFATE
DQTEGMSAFVERREKNFKNK

Sequences:

>Translated_260_residues
MELNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAFVAGADIAEMKDLNEEEGK
EFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACDIRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKE
LIYTGDMIKADEALRIGLVNKVVEPENLMEEAMSLAKKISNNAPIAVKLCKDAINRGIQVDIDSAVVIEAEDFGKCFATE
DQTEGMSAFVERREKNFKNK
>Mature_260_residues
MELNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDKAFVAGADIAEMKDLNEEEGK
EFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACDIRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKE
LIYTGDMIKADEALRIGLVNKVVEPENLMEEAMSLAKKISNNAPIAVKLCKDAINRGIQVDIDSAVVIEAEDFGKCFATE
DQTEGMSAFVERREKNFKNK

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=44.9612403100775, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=36.6141732283465, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=32.6693227091633, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI20127408, Length=218, Percent_Identity=35.3211009174312, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI68989263, Length=233, Percent_Identity=33.9055793991416, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI150378541, Length=249, Percent_Identity=34.136546184739, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI37594469, Length=243, Percent_Identity=32.5102880658436, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI37594471, Length=243, Percent_Identity=32.5102880658436, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI70995211, Length=208, Percent_Identity=33.6538461538462, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=28.6919831223629, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=28.6919831223629, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI221136756, Length=257, Percent_Identity=26.0700389105058, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI221136753, Length=257, Percent_Identity=26.0700389105058, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI221307494, Length=257, Percent_Identity=26.0700389105058, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260274832, Length=254, Percent_Identity=26.3779527559055, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI45643119, Length=254, Percent_Identity=26.3779527559055, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI260275230, Length=254, Percent_Identity=26.3779527559055, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI25453481, Length=256, Percent_Identity=25.390625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI51538573, Length=256, Percent_Identity=25.390625, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI261878539, Length=147, Percent_Identity=36.0544217687075, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI4757968, Length=257, Percent_Identity=26.0700389105058, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI49355787, Length=257, Percent_Identity=26.0700389105058, Blast_Score=88, Evalue=8e-18,
Organism=Homo sapiens, GI4757966, Length=264, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI51538581, Length=264, Percent_Identity=25, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI116235487, Length=206, Percent_Identity=28.1553398058252, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI157694520, Length=192, Percent_Identity=28.6458333333333, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI62530384, Length=254, Percent_Identity=23.6220472440945, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787659, Length=260, Percent_Identity=36.9230769230769, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI221142681, Length=255, Percent_Identity=36.4705882352941, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1790281, Length=194, Percent_Identity=42.2680412371134, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=32.4528301886792, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1788682, Length=201, Percent_Identity=37.3134328358209, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=32.8185328185328, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=29.4820717131474, Blast_Score=117, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=42.741935483871, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=39.2, Blast_Score=190, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17540714, Length=228, Percent_Identity=43.421052631579, Blast_Score=177, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=37.3626373626374, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17534483, Length=211, Percent_Identity=33.175355450237, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=256, Percent_Identity=30.078125, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17536985, Length=246, Percent_Identity=28.8617886178862, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=228, Percent_Identity=34.2105263157895, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=228, Percent_Identity=34.2105263157895, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=228, Percent_Identity=34.2105263157895, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25144157, Length=212, Percent_Identity=33.9622641509434, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI25144160, Length=212, Percent_Identity=33.9622641509434, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17549921, Length=217, Percent_Identity=30.8755760368664, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=257, Percent_Identity=28.7937743190661, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141363, Length=197, Percent_Identity=29.4416243654822, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=253, Percent_Identity=25.6916996047431, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=31.6062176165803, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=45.5645161290323, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=45.5645161290323, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=39.0438247011952, Blast_Score=177, Evalue=8e-45,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=27.2030651340996, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=34.1836734693878, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21357171, Length=198, Percent_Identity=33.8383838383838, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19920382, Length=215, Percent_Identity=29.7674418604651, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921000, Length=174, Percent_Identity=38.5057471264368, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24583077, Length=174, Percent_Identity=38.5057471264368, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24583079, Length=174, Percent_Identity=38.5057471264368, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI28571729, Length=160, Percent_Identity=35, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28571730, Length=160, Percent_Identity=35, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24654903, Length=187, Percent_Identity=26.7379679144385, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583165, Length=212, Percent_Identity=28.3018867924528, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921018, Length=215, Percent_Identity=30.6976744186047, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28184; Mature: 28184

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDK
CCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
AFVAGADIAEMKDLNEEEGKEFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACD
EEEECCCHHHHHCCCHHCCCHHCHHHHHHHHHHHHCCCCEEEEECCEEECCCCEEEEEEE
IRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKELIYTGDMIKADEALRIGLVN
EEEEECHHHHCCCCCCCEECCCCCCHHHCCHHCCCCCCCEEEEECCEEECCCHHHHHHHH
KVVEPENLMEEAMSLAKKISNNAPIAVKLCKDAINRGIQVDIDSAVVIEAEDFGKCFATE
HHCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCEEEEECCEEEEEEHHCCCEECCC
DQTEGMSAFVERREKNFKNK
CCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELNNVIFEKEGNIGVLTINRPKALNALNSETLKDLDTAIDHIEKQDDIYVVILTGAGDK
CCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
AFVAGADIAEMKDLNEEEGKEFGLLGNKVFRRLENLDKPVIAAINGFALGGGCEISMACD
EEEECCCHHHHHCCCHHCCCHHCHHHHHHHHHHHHCCCCEEEEECCEEECCCCEEEEEEE
IRIATTKAKFAQPEVGLGITPGFGGTQRLPRIVGPGKAKELIYTGDMIKADEALRIGLVN
EEEEECHHHHCCCCCCCEECCCCCCHHHCCHHCCCCCCCEEEEECCEEECCCHHHHHHHH
KVVEPENLMEEAMSLAKKISNNAPIAVKLCKDAINRGIQVDIDSAVVIEAEDFGKCFATE
HHCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCCEEEEECCEEEEEEHHCCCEECCC
DQTEGMSAFVERREKNFKNK
CCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]