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The map label for this gene is hofD [H]

Identifier: 18311269

GI number: 18311269

Start: 2631883

End: 2632626

Strand: Reverse

Name: hofD [H]

Synonym: CPE2287

Alternate gene names: 18311269

Gene position: 2632626-2631883 (Counterclockwise)

Preceding gene: 18311270

Following gene: 18311268

Centisome position: 86.84

GC content: 26.61

Gene sequence:

>744_bases
GTGTCTATAATATTTTTTTTATTTGGATTAATAATTGGAAGTTTTTTAAATGTGTGTATTTTTAGAATTCCCGCAGGGGA
ATCTATAGCATTTCCGCCTTCACATTGTGGTACATGCAAAAAACAATTAAAGCCTATAGATTTAGTACCTGTTTTTAGTT
ATTTGTTTTTAGGTGGAAAGTGTAGATATTGTAAGAGCAAAATATCTATAAGATATCCACTAATAGAATTGCTTACAGGT
TTTTTATATTTTTATGTATATAAATATTATGGTATATCTTTTTTGACAGTTAAGTACATAGTCTTAATTACATTTCTCAT
AGTAATTTCATTTATAGATTACGATACCCAAGATGTTTATGCAGTTACTACTTACCCAGCTGTAATACTTGGGATTATTT
TTGCCCTAATAGAAAAGTTTTATTTTGGAGAAAACTTATTAAATTACTTTATGGGATTAGTAATATCAGCTTTAGTAATA
TTTTTAATTAGTAAATTAACTGGAGCTATGGGGAGTGGAGATATAGAGATACATGCCATAGCAGGAATTTTTTTAGGGTG
GAAATTAGCAATAATAAATATATTTTTATCATTTATTATTGGTGGAATAATAGCAGTATTAGCTATTTTGTTTAAACAAA
AGAAAAAAGGTGATTATATAGCCTTTGGACCAGCTATAGGAATATCTACTATTATTTTAATATTTTTTGGAAACATAATA
ATACCAATATATTTTGGTGTGTGA

Upstream 100 bases:

>100_bases
AAAAAGCTTAATCAAAAATGTTTACAAAAAATTTCAAGATGTTTATAATAATTATGAAATATTAATGAGTAATTCATTTT
TGGATGATTTGGGAGGCAAT

Downstream 100 bases:

>100_bases
AAAATTTAATATACTAGTTTAGATTAAGTTTTATAGTTAAATTTTTAACTTGAAAAGGAGGTATTTTCTTTGATTAGTTA
TCAGAAAAAGCGTTTAGGAG

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSIIFFLFGLIIGSFLNVCIFRIPAGESIAFPPSHCGTCKKQLKPIDLVPVFSYLFLGGKCRYCKSKISIRYPLIELLTG
FLYFYVYKYYGISFLTVKYIVLITFLIVISFIDYDTQDVYAVTTYPAVILGIIFALIEKFYFGENLLNYFMGLVISALVI
FLISKLTGAMGSGDIEIHAIAGIFLGWKLAIINIFLSFIIGGIIAVLAILFKQKKKGDYIAFGPAIGISTIILIFFGNII
IPIYFGV

Sequences:

>Translated_247_residues
MSIIFFLFGLIIGSFLNVCIFRIPAGESIAFPPSHCGTCKKQLKPIDLVPVFSYLFLGGKCRYCKSKISIRYPLIELLTG
FLYFYVYKYYGISFLTVKYIVLITFLIVISFIDYDTQDVYAVTTYPAVILGIIFALIEKFYFGENLLNYFMGLVISALVI
FLISKLTGAMGSGDIEIHAIAGIFLGWKLAIINIFLSFIIGGIIAVLAILFKQKKKGDYIAFGPAIGISTIILIFFGNII
IPIYFGV
>Mature_246_residues
SIIFFLFGLIIGSFLNVCIFRIPAGESIAFPPSHCGTCKKQLKPIDLVPVFSYLFLGGKCRYCKSKISIRYPLIELLTGF
LYFYVYKYYGISFLTVKYIVLITFLIVISFIDYDTQDVYAVTTYPAVILGIIFALIEKFYFGENLLNYFMGLVISALVIF
LISKLTGAMGSGDIEIHAIAGIFLGWKLAIINIFLSFIIGGIIAVLAILFKQKKKGDYIAFGPAIGISTIILIFFGNIII
PIYFGV

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=261, Percent_Identity=30.6513409961686, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 27580; Mature: 27449

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCC
>Mature Secondary Structure 
SIIFFLFGLIIGSFLNVCIFRIPAGESIAFPPSHCGTCKKQLKPIDLVPVFSYLFLGGK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCC
CRYCKSKISIRYPLIELLTGFLYFYVYKYYGISFLTVKYIVLITFLIVISFIDYDTQDVY
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
AVTTYPAVILGIIFALIEKFYFGENLLNYFMGLVISALVIFLISKLTGAMGSGDIEIHAI
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
AGIFLGWKLAIINIFLSFIIGGIIAVLAILFKQKKKGDYIAFGPAIGISTIILIFFGNII
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHH
IPIYFGV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]