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The map label for this gene is pcrA [H]

Identifier: 18311242

GI number: 18311242

Start: 2596086

End: 2598341

Strand: Reverse

Name: pcrA [H]

Synonym: CPE2260

Alternate gene names: 18311242

Gene position: 2598341-2596086 (Counterclockwise)

Preceding gene: 18311243

Following gene: 18311241

Centisome position: 85.71

GC content: 29.61

Gene sequence:

>2256_bases
ATGGATTTAAAAAGCTTGCTTAATAAAGAACAATATGAAGCAGCAACCACAATTGATGGTCAAGTACTTATATTAGCAGG
AGCAGGTTCTGGTAAAACTAGAGTACTTACACACAGAATAGCATACATGATAGAAAATGATATAAAACCTTATAATATAT
TAGCGATAACATTTACAAATAAAGCAGCAGGGGAAATGAGAGAGAGAGTAAAATCTTTAGTTGGTGAAGTGGCAAACAAC
ATGTGGATATCAACATTTCATTCATCTTGCGTGAGAATTTTAAGAAGAGAAATAGATAAATTAGGTTATAGTAAAGACTT
TACAATATATGATTCATCAGATCAGAAAACATTAATAAAACTTGTAATGAAAGAGCTTAATATTAATGAAAAAGAGATAA
CTGATTTAGAGATTTTAGGTACTATAGGAAAAGCAAAGGATAATATCCAAAGTGCACAAAGTTTTAAGAAAGAAAATGAA
GATAACTTTAGAAAAAATAAAATAGCAGATGCATATCTTTTATACCAAAAGAAATTAAAAGAAAATAATGCCCTAGATTT
TGATGATTTAATAGTAAAAACTGTTGAGTTATTTAGAAAGCATAATGATGTATTAGAGTTTTATCAAAATAAGTTTAAAT
ACATAATGATAGATGAATATCAAGATACAAATAAAGCTCAATATGAATTTGCTAAGCTTTTAGCTGCAAAACACAAAAAT
ATATGCGTAGTTGGTGATGATGACCAATGTATTTATGCTTGGAGAGGGGCAGATATAAGAAATATATTAGATTTTGAAAA
GGATTATCCAGAGGCTAAAGTTGTTAAACTTGAGCAAAACTATAGATCAAAAGGAAATATTTTAGAAGCAGCTAATAAAG
TTATAAAAAATAATGCAGAGAGAAAATTTAAGATTTTAAGAACTGAGCAAGTAGAGGGAGAAAAAATAAGTATTCATAGA
GCTTACTCAGATAAAATGGAGGCAGCTTTTGTTGTTTCAGAGATAGAAAAATTAAAAAATGATAATGGATATAAGTATAA
GGACTTTGCTGTTCTTTACAGAACAAATGCACAGTCTCGTAATTTTGAGGAAGCTTTAAGAAGAGCAGGAATTCCATATA
GAATTTTTGGAGGACTAAAATTCTATGATAGAAAAGAGATAAAAGATATTCTTGCATACTTAAAAATAATAGTAAATCCT
AAAGATTCTATAAGTTTAAGAAGAATAATAAATGTTCCTAAAAGAAGCATTGGAGATACTACTGTAGAAAAACTTCAAAA
TCATGCAACTGAAATAGATGATACATTATACAATGTTTTATTAGATGTAGACTATGTGCCAGGATTAACAGCTAGAAGCA
TAAATCCTATTAAGAAGTTTACTGATATGATGGAAGAAATAATGGTTATGTCAGAGCAATTAAGTGTATCTCAATTAATT
GAATATGTTTTGGAAAAAACAGGTTATTTAAAATCACTTAAAGATTCAAAGCTTATAGAGGATCAAAGTAGAATAGAAAA
CTTAGAGGAATTAGTTTCTGATGCTGTTGAATTTGAAAAGAGTAATGAAGAAGATAAATCATTATCAGCTTACTTAGAGA
AGGTTGCCCTTGTTCAAGATATGGATGATTTAGAAGCAGAAGATAATTATGTAATGCTAATGACAGTTCACAGCTCAAAG
GGACTAGAATTCCCAACTGTTTTCTTAGTTGGTATGGAAAATGGAATATTCCCAAATGCAGCTTGCTTTGAACATGATAA
TCAAATGGAAGAGGCTAGAAGACTTTGCTATGTTGGAATAACTAGAGCTGAGGAAAAGTTATATATGACATCAGCTGAAA
CAAGAATGATATTTGGTAAAACAGTAGCTTATGGACAATCAGATTTTATATCAGAAATTCCAGCTAACATAAAAGACTAT
GTAAATATCAATGGTAAAAGTTCAAGTAACTCTTTCAAAAATACTTCACAAACTTATGGAGAGACTAGAAAAACTTATAA
TCCTCACAGTATAAGAGGAGGAATGAGTGTTTCTAAAGCAAATGAATCCCTAAATAAGACTTTATCAGGAAAATCTTCAG
GTAATGCAGGAATAGATGAATTAACCATGGGAAGAAAAGTTAAGCATCCTAAGTTTGGTGTGGGAACATTAATAACAAAG
AGTAAAGTTGGAGATGACTATAAGCTTACAATTGCCTTTGATAGTCAAGGAGTTAAACATTTAATGTTTAGCTTTGCACC
ACTAGAGCTTCTTTAA

Upstream 100 bases:

>100_bases
TATTAATCATGTAAAAGAATAATATGTTTGATAGAATATAATATATAGATTTATATGGTATAATAAAGTTTAGTTTTATT
GTAGGAAGAGGAGTGATCTA

Downstream 100 bases:

>100_bases
TATAGAAATTTACTTTAATAAGGAGTAGATGAGTAAGATGGATAAGAAAAAATTAATAGAAGAGTTAGTTGAAGAGTTAA
ATAAATATGCTTATGAATAT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MDLKSLLNKEQYEAATTIDGQVLILAGAGSGKTRVLTHRIAYMIENDIKPYNILAITFTNKAAGEMRERVKSLVGEVANN
MWISTFHSSCVRILRREIDKLGYSKDFTIYDSSDQKTLIKLVMKELNINEKEITDLEILGTIGKAKDNIQSAQSFKKENE
DNFRKNKIADAYLLYQKKLKENNALDFDDLIVKTVELFRKHNDVLEFYQNKFKYIMIDEYQDTNKAQYEFAKLLAAKHKN
ICVVGDDDQCIYAWRGADIRNILDFEKDYPEAKVVKLEQNYRSKGNILEAANKVIKNNAERKFKILRTEQVEGEKISIHR
AYSDKMEAAFVVSEIEKLKNDNGYKYKDFAVLYRTNAQSRNFEEALRRAGIPYRIFGGLKFYDRKEIKDILAYLKIIVNP
KDSISLRRIINVPKRSIGDTTVEKLQNHATEIDDTLYNVLLDVDYVPGLTARSINPIKKFTDMMEEIMVMSEQLSVSQLI
EYVLEKTGYLKSLKDSKLIEDQSRIENLEELVSDAVEFEKSNEEDKSLSAYLEKVALVQDMDDLEAEDNYVMLMTVHSSK
GLEFPTVFLVGMENGIFPNAACFEHDNQMEEARRLCYVGITRAEEKLYMTSAETRMIFGKTVAYGQSDFISEIPANIKDY
VNINGKSSSNSFKNTSQTYGETRKTYNPHSIRGGMSVSKANESLNKTLSGKSSGNAGIDELTMGRKVKHPKFGVGTLITK
SKVGDDYKLTIAFDSQGVKHLMFSFAPLELL

Sequences:

>Translated_751_residues
MDLKSLLNKEQYEAATTIDGQVLILAGAGSGKTRVLTHRIAYMIENDIKPYNILAITFTNKAAGEMRERVKSLVGEVANN
MWISTFHSSCVRILRREIDKLGYSKDFTIYDSSDQKTLIKLVMKELNINEKEITDLEILGTIGKAKDNIQSAQSFKKENE
DNFRKNKIADAYLLYQKKLKENNALDFDDLIVKTVELFRKHNDVLEFYQNKFKYIMIDEYQDTNKAQYEFAKLLAAKHKN
ICVVGDDDQCIYAWRGADIRNILDFEKDYPEAKVVKLEQNYRSKGNILEAANKVIKNNAERKFKILRTEQVEGEKISIHR
AYSDKMEAAFVVSEIEKLKNDNGYKYKDFAVLYRTNAQSRNFEEALRRAGIPYRIFGGLKFYDRKEIKDILAYLKIIVNP
KDSISLRRIINVPKRSIGDTTVEKLQNHATEIDDTLYNVLLDVDYVPGLTARSINPIKKFTDMMEEIMVMSEQLSVSQLI
EYVLEKTGYLKSLKDSKLIEDQSRIENLEELVSDAVEFEKSNEEDKSLSAYLEKVALVQDMDDLEAEDNYVMLMTVHSSK
GLEFPTVFLVGMENGIFPNAACFEHDNQMEEARRLCYVGITRAEEKLYMTSAETRMIFGKTVAYGQSDFISEIPANIKDY
VNINGKSSSNSFKNTSQTYGETRKTYNPHSIRGGMSVSKANESLNKTLSGKSSGNAGIDELTMGRKVKHPKFGVGTLITK
SKVGDDYKLTIAFDSQGVKHLMFSFAPLELL
>Mature_751_residues
MDLKSLLNKEQYEAATTIDGQVLILAGAGSGKTRVLTHRIAYMIENDIKPYNILAITFTNKAAGEMRERVKSLVGEVANN
MWISTFHSSCVRILRREIDKLGYSKDFTIYDSSDQKTLIKLVMKELNINEKEITDLEILGTIGKAKDNIQSAQSFKKENE
DNFRKNKIADAYLLYQKKLKENNALDFDDLIVKTVELFRKHNDVLEFYQNKFKYIMIDEYQDTNKAQYEFAKLLAAKHKN
ICVVGDDDQCIYAWRGADIRNILDFEKDYPEAKVVKLEQNYRSKGNILEAANKVIKNNAERKFKILRTEQVEGEKISIHR
AYSDKMEAAFVVSEIEKLKNDNGYKYKDFAVLYRTNAQSRNFEEALRRAGIPYRIFGGLKFYDRKEIKDILAYLKIIVNP
KDSISLRRIINVPKRSIGDTTVEKLQNHATEIDDTLYNVLLDVDYVPGLTARSINPIKKFTDMMEEIMVMSEQLSVSQLI
EYVLEKTGYLKSLKDSKLIEDQSRIENLEELVSDAVEFEKSNEEDKSLSAYLEKVALVQDMDDLEAEDNYVMLMTVHSSK
GLEFPTVFLVGMENGIFPNAACFEHDNQMEEARRLCYVGITRAEEKLYMTSAETRMIFGKTVAYGQSDFISEIPANIKDY
VNINGKSSSNSFKNTSQTYGETRKTYNPHSIRGGMSVSKANESLNKTLSGKSSGNAGIDELTMGRKVKHPKFGVGTLITK
SKVGDDYKLTIAFDSQGVKHLMFSFAPLELL

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=759, Percent_Identity=37.5494071146245, Blast_Score=520, Evalue=1e-148,
Organism=Escherichia coli, GI48994965, Length=643, Percent_Identity=39.1912908242613, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=27.0588235294118, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=740, Percent_Identity=28.2432432432432, Blast_Score=210, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6324477, Length=748, Percent_Identity=25.4010695187166, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85729; Mature: 85729

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKSLLNKEQYEAATTIDGQVLILAGAGSGKTRVLTHRIAYMIENDIKPYNILAITFTN
CCHHHHHCHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECC
KAAGEMRERVKSLVGEVANNMWISTFHSSCVRILRREIDKLGYSKDFTIYDSSDQKTLIK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
LVMKELNINEKEITDLEILGTIGKAKDNIQSAQSFKKENEDNFRKNKIADAYLLYQKKLK
HHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
ENNALDFDDLIVKTVELFRKHNDVLEFYQNKFKYIMIDEYQDTNKAQYEFAKLLAAKHKN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
ICVVGDDDQCIYAWRGADIRNILDFEKDYPEAKVVKLEQNYRSKGNILEAANKVIKNNAE
EEEECCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHCCCH
RKFKILRTEQVEGEKISIHRAYSDKMEAAFVVSEIEKLKNDNGYKYKDFAVLYRTNAQSR
HHEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCC
NFEEALRRAGIPYRIFGGLKFYDRKEIKDILAYLKIIVNPKDSISLRRIINVPKRSIGDT
CHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHH
TVEKLQNHATEIDDTLYNVLLDVDYVPGLTARSINPIKKFTDMMEEIMVMSEQLSVSQLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
EYVLEKTGYLKSLKDSKLIEDQSRIENLEELVSDAVEFEKSNEEDKSLSAYLEKVALVQD
HHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
MDDLEAEDNYVMLMTVHSSKGLEFPTVFLVGMENGIFPNAACFEHDNQMEEARRLCYVGI
HHHCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHEE
TRAEEKLYMTSAETRMIFGKTVAYGQSDFISEIPANIKDYVNINGKSSSNSFKNTSQTYG
ECCCHHEEEECCCCEEEEECHHHCCCHHHHHHCCCCCHHHEEECCCCCCCCCCCCHHHHH
ETRKTYNPHSIRGGMSVSKANESLNKTLSGKSSGNAGIDELTMGRKVKHPKFGVGTLITK
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEC
SKVGDDYKLTIAFDSQGVKHLMFSFAPLELL
CCCCCCEEEEEEECCCCHHHHHHHCCCHHCC
>Mature Secondary Structure
MDLKSLLNKEQYEAATTIDGQVLILAGAGSGKTRVLTHRIAYMIENDIKPYNILAITFTN
CCHHHHHCHHHHHHHEECCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECC
KAAGEMRERVKSLVGEVANNMWISTFHSSCVRILRREIDKLGYSKDFTIYDSSDQKTLIK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
LVMKELNINEKEITDLEILGTIGKAKDNIQSAQSFKKENEDNFRKNKIADAYLLYQKKLK
HHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
ENNALDFDDLIVKTVELFRKHNDVLEFYQNKFKYIMIDEYQDTNKAQYEFAKLLAAKHKN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
ICVVGDDDQCIYAWRGADIRNILDFEKDYPEAKVVKLEQNYRSKGNILEAANKVIKNNAE
EEEECCCCCEEEEECCCCHHHHHHHHHCCCCCEEEEEHHHCCCCCCHHHHHHHHHHCCCH
RKFKILRTEQVEGEKISIHRAYSDKMEAAFVVSEIEKLKNDNGYKYKDFAVLYRTNAQSR
HHEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCC
NFEEALRRAGIPYRIFGGLKFYDRKEIKDILAYLKIIVNPKDSISLRRIINVPKRSIGDT
CHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCHH
TVEKLQNHATEIDDTLYNVLLDVDYVPGLTARSINPIKKFTDMMEEIMVMSEQLSVSQLI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
EYVLEKTGYLKSLKDSKLIEDQSRIENLEELVSDAVEFEKSNEEDKSLSAYLEKVALVQD
HHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
MDDLEAEDNYVMLMTVHSSKGLEFPTVFLVGMENGIFPNAACFEHDNQMEEARRLCYVGI
HHHCCCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHEE
TRAEEKLYMTSAETRMIFGKTVAYGQSDFISEIPANIKDYVNINGKSSSNSFKNTSQTYG
ECCCHHEEEECCCCEEEEECHHHCCCHHHHHHCCCCCHHHEEECCCCCCCCCCCCHHHHH
ETRKTYNPHSIRGGMSVSKANESLNKTLSGKSSGNAGIDELTMGRKVKHPKFGVGTLITK
HHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEC
SKVGDDYKLTIAFDSQGVKHLMFSFAPLELL
CCCCCCEEEEEEECCCCHHHHHHHCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]