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The map label for this gene is ligA [H]

Identifier: 18311241

GI number: 18311241

Start: 2594060

End: 2596057

Strand: Reverse

Name: ligA [H]

Synonym: CPE2259

Alternate gene names: 18311241

Gene position: 2596057-2594060 (Counterclockwise)

Preceding gene: 18311242

Following gene: 18311239

Centisome position: 85.64

GC content: 30.53

Gene sequence:

>1998_bases
ATGAGTAAGATGGATAAGAAAAAATTAATAGAAGAGTTAGTTGAAGAGTTAAATAAATATGCTTATGAATATTATGTTTT
AGGAAATTCTTCAGTAACAGATAAAGATTATGATAAAAAATACTATGAATTAGTAGATTTAGAAAAGGAAACAGGATATA
AATTACCCTATTCACCTACTCAAAGAGTTGGTGATGTAATTCTTCCTGAGTTTAAAAAATACACTCATAAAGCTAGACTT
TGGAGTTTAGACAAAGCTCAAACTTTAGAGGAAATTAGAGAGTGGCATAATAGAAATGTTAAATTCCTTGAAGAATATAA
TAGAACTTCAGATGAAGAATTACCACCATTAAAATATATTTTAACTAAAAAGTTTGATGGATTAACAATTAATCTTTCCT
ATGATGAAAATGGAGTATTAGTTACTGGAGCAACTAGAGGAACTGGGGCTATTGGTGAAGATGTGACAGCTCAGGTTAAA
ACTATAAAATCAATTCCATTAAAAATTGATTGTCATGATTTTCTAGAAATTCATGGAGAGGCTATTATGACTACAGAAGC
CTTTGAAAAATACAATAGTGAAGCAGAGACTCCTCTTAAGAATTTAAGAAATGGAGCAGCGGGAGCTTTAAGAAATTTAA
ATGTAGCAGAGACAGCTAAAAGAAATTTATCAGCTTTCTTCTATGATGTTGGATATAAGGAAGGAGCTCCTTTTAAAACT
TATATGGAAATGTTAAATTTCATAAAAACTAAGGGATTTCCAATGGATGATTATATTAGGGAATGTACAACTTTAGATGA
AATTCAAAAAGAAATTGATTATATAAGAGATATAAGATTTGATTTAAATTATGATATAGATGGATTAGTTATAGCTATAG
ATGATATAAGAACTAGAGATCTTTTAGGATACACAGTTAAATTTCCTAAGTGGGCTATTGCATATAAATTTGAAGCACAG
GAGGCTACAACTAAGCTTTTAGATGTTGAATGGAATGTAGGTAGAAGCGGAAGAGTATCTCCCACAGCTATCTTAGAGCC
AGTAGAGTTAGCTGGAGTTACTGTTAAGAGGGCTACATTAAACAATATGGATGATATAGCTAGAAAAGGAGTAAGATTAG
GGGCTGAAGTATTTGTAAGAAGAAGCAATGATGTTATTCCAGAAATAATGGGAGTAGTTCCTGAAAGCTTAGAAGGTACA
AAGGAAATTGAAGAACCAAAGGTATGTCCAGCTTGTGGAGCACATTTAGTTCATGAAGGAGTTCATATCTATTGTGAAAA
CACTTTAGGATGTAAGCCTCAAATGGTTAAAACTATAGTTCACTTTGCTGGAAGAGAAGCTATGAATATAGCTGGTTTTT
CAGAAAAAACAGCGGAGCAATTATTTGAAAAGCTTGATATAAGAGATATATCAGATCTTTATAAATTAGAATATGAAAAA
TTATTAGATTTAGATAAGTTTGGGCCAAAGAAAGCACAAAATCTTTTAGATGCCATAGAAAAAAGTAAAGATTGTACTCT
AGAAGCATTTCTATATTCCTTAGGAATTCCTAACGTAGGAGTTAAAACTGCTAAGGATTTAGTAAAGAGATTTGAGTCTT
TAGAAAACCTAGAGAAGGCTACTTTTGAAGAATTAGTATCTGTCCAAGATGTTGGAGATATAGTTGCTAGAAGTATAATA
GAATTCTTTAAAGAGGAAAGAACTTTAAAAGTAATAAATGAATTATTATCCCTTGGAGTAAATCCTCATTACGAGAAAAA
AGAAGTTTTAGAAAGTCCATTTATGGGAAAAACAGTAGTAGTAACTGGTACTTTAGAAAACTATTCTAGAACATCAATAA
AAGAAAAATTAGAATCTTTAGGAGCAAAGGTTTCAGGAAGTGTAAGTAAAAAGACTGATTTTGTAATAGCAGGAGAAGCA
GCTGGTTCAAAATATGATAAAGCTAAATCTTTAGGTGTTACTATATTAAGTGAAGAAGAATTTGAAAATATGATATAA

Upstream 100 bases:

>100_bases
CTTACAATTGCCTTTGATAGTCAAGGAGTTAAACATTTAATGTTTAGCTTTGCACCACTAGAGCTTCTTTAATATAGAAA
TTTACTTTAATAAGGAGTAG

Downstream 100 bases:

>100_bases
AAAAAATAAAAGCTATGTGAGTAATTTCACATGGCTTTTATTTTTCCTCTATATTTGAGTTTTCTTTTATTTCCTCTGAT
ATATTTGTATCCTCTTCTGA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 665; Mature: 664

Protein sequence:

>665_residues
MSKMDKKKLIEELVEELNKYAYEYYVLGNSSVTDKDYDKKYYELVDLEKETGYKLPYSPTQRVGDVILPEFKKYTHKARL
WSLDKAQTLEEIREWHNRNVKFLEEYNRTSDEELPPLKYILTKKFDGLTINLSYDENGVLVTGATRGTGAIGEDVTAQVK
TIKSIPLKIDCHDFLEIHGEAIMTTEAFEKYNSEAETPLKNLRNGAAGALRNLNVAETAKRNLSAFFYDVGYKEGAPFKT
YMEMLNFIKTKGFPMDDYIRECTTLDEIQKEIDYIRDIRFDLNYDIDGLVIAIDDIRTRDLLGYTVKFPKWAIAYKFEAQ
EATTKLLDVEWNVGRSGRVSPTAILEPVELAGVTVKRATLNNMDDIARKGVRLGAEVFVRRSNDVIPEIMGVVPESLEGT
KEIEEPKVCPACGAHLVHEGVHIYCENTLGCKPQMVKTIVHFAGREAMNIAGFSEKTAEQLFEKLDIRDISDLYKLEYEK
LLDLDKFGPKKAQNLLDAIEKSKDCTLEAFLYSLGIPNVGVKTAKDLVKRFESLENLEKATFEELVSVQDVGDIVARSII
EFFKEERTLKVINELLSLGVNPHYEKKEVLESPFMGKTVVVTGTLENYSRTSIKEKLESLGAKVSGSVSKKTDFVIAGEA
AGSKYDKAKSLGVTILSEEEFENMI

Sequences:

>Translated_665_residues
MSKMDKKKLIEELVEELNKYAYEYYVLGNSSVTDKDYDKKYYELVDLEKETGYKLPYSPTQRVGDVILPEFKKYTHKARL
WSLDKAQTLEEIREWHNRNVKFLEEYNRTSDEELPPLKYILTKKFDGLTINLSYDENGVLVTGATRGTGAIGEDVTAQVK
TIKSIPLKIDCHDFLEIHGEAIMTTEAFEKYNSEAETPLKNLRNGAAGALRNLNVAETAKRNLSAFFYDVGYKEGAPFKT
YMEMLNFIKTKGFPMDDYIRECTTLDEIQKEIDYIRDIRFDLNYDIDGLVIAIDDIRTRDLLGYTVKFPKWAIAYKFEAQ
EATTKLLDVEWNVGRSGRVSPTAILEPVELAGVTVKRATLNNMDDIARKGVRLGAEVFVRRSNDVIPEIMGVVPESLEGT
KEIEEPKVCPACGAHLVHEGVHIYCENTLGCKPQMVKTIVHFAGREAMNIAGFSEKTAEQLFEKLDIRDISDLYKLEYEK
LLDLDKFGPKKAQNLLDAIEKSKDCTLEAFLYSLGIPNVGVKTAKDLVKRFESLENLEKATFEELVSVQDVGDIVARSII
EFFKEERTLKVINELLSLGVNPHYEKKEVLESPFMGKTVVVTGTLENYSRTSIKEKLESLGAKVSGSVSKKTDFVIAGEA
AGSKYDKAKSLGVTILSEEEFENMI
>Mature_664_residues
SKMDKKKLIEELVEELNKYAYEYYVLGNSSVTDKDYDKKYYELVDLEKETGYKLPYSPTQRVGDVILPEFKKYTHKARLW
SLDKAQTLEEIREWHNRNVKFLEEYNRTSDEELPPLKYILTKKFDGLTINLSYDENGVLVTGATRGTGAIGEDVTAQVKT
IKSIPLKIDCHDFLEIHGEAIMTTEAFEKYNSEAETPLKNLRNGAAGALRNLNVAETAKRNLSAFFYDVGYKEGAPFKTY
MEMLNFIKTKGFPMDDYIRECTTLDEIQKEIDYIRDIRFDLNYDIDGLVIAIDDIRTRDLLGYTVKFPKWAIAYKFEAQE
ATTKLLDVEWNVGRSGRVSPTAILEPVELAGVTVKRATLNNMDDIARKGVRLGAEVFVRRSNDVIPEIMGVVPESLEGTK
EIEEPKVCPACGAHLVHEGVHIYCENTLGCKPQMVKTIVHFAGREAMNIAGFSEKTAEQLFEKLDIRDISDLYKLEYEKL
LDLDKFGPKKAQNLLDAIEKSKDCTLEAFLYSLGIPNVGVKTAKDLVKRFESLENLEKATFEELVSVQDVGDIVARSIIE
FFKEERTLKVINELLSLGVNPHYEKKEVLESPFMGKTVVVTGTLENYSRTSIKEKLESLGAKVSGSVSKKTDFVIAGEAA
GSKYDKAKSLGVTILSEEEFENMI

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=41.4307004470939, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI87082305, Length=574, Percent_Identity=21.4285714285714, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75311; Mature: 75180

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50172 BRCT ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKMDKKKLIEELVEELNKYAYEYYVLGNSSVTDKDYDKKYYELVDLEKETGYKLPYSPT
CCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCHH
QRVGDVILPEFKKYTHKARLWSLDKAQTLEEIREWHNRNVKFLEEYNRTSDEELPPLKYI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHH
LTKKFDGLTINLSYDENGVLVTGATRGTGAIGEDVTAQVKTIKSIPLKIDCHDFLEIHGE
HHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCEEECHHHHHHHCCC
AIMTTEAFEKYNSEAETPLKNLRNGAAGALRNLNVAETAKRNLSAFFYDVGYKEGAPFKT
CEEEHHHHHHHCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCCHHH
YMEMLNFIKTKGFPMDDYIRECTTLDEIQKEIDYIRDIRFDLNYDIDGLVIAIDDIRTRD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
LLGYTVKFPKWAIAYKFEAQEATTKLLDVEWNVGRSGRVSPTAILEPVELAGVTVKRATL
HHCEEEECCCEEEEEEEEHHHHHHHHEEEEECCCCCCCCCHHHHCCCHHHHCCEEEHHHH
NNMDDIARKGVRLGAEVFVRRSNDVIPEIMGVVPESLEGTKEIEEPKVCPACGAHLVHEG
CCHHHHHHHHHHHCHHHEEECCCCHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHCC
VHIYCENTLGCKPQMVKTIVHFAGREAMNIAGFSEKTAEQLFEKLDIRDISDLYKLEYEK
EEEEECCCCCCCHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LLDLDKFGPKKAQNLLDAIEKSKDCTLEAFLYSLGIPNVGVKTAKDLVKRFESLENLEKA
HHCHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TFEELVSVQDVGDIVARSIIEFFKEERTLKVINELLSLGVNPHYEKKEVLESPFMGKTVV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEE
VTGTLENYSRTSIKEKLESLGAKVSGSVSKKTDFVIAGEAAGSKYDKAKSLGVTILSEEE
EEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCEEEEEHHH
FENMI
HHCCC
>Mature Secondary Structure 
SKMDKKKLIEELVEELNKYAYEYYVLGNSSVTDKDYDKKYYELVDLEKETGYKLPYSPT
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHCHHHCCCCCCCCHH
QRVGDVILPEFKKYTHKARLWSLDKAQTLEEIREWHNRNVKFLEEYNRTSDEELPPLKYI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHH
LTKKFDGLTINLSYDENGVLVTGATRGTGAIGEDVTAQVKTIKSIPLKIDCHDFLEIHGE
HHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCEEECHHHHHHHCCC
AIMTTEAFEKYNSEAETPLKNLRNGAAGALRNLNVAETAKRNLSAFFYDVGYKEGAPFKT
CEEEHHHHHHHCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHHHHHEEECCCCCCCCHHH
YMEMLNFIKTKGFPMDDYIRECTTLDEIQKEIDYIRDIRFDLNYDIDGLVIAIDDIRTRD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
LLGYTVKFPKWAIAYKFEAQEATTKLLDVEWNVGRSGRVSPTAILEPVELAGVTVKRATL
HHCEEEECCCEEEEEEEEHHHHHHHHEEEEECCCCCCCCCHHHHCCCHHHHCCEEEHHHH
NNMDDIARKGVRLGAEVFVRRSNDVIPEIMGVVPESLEGTKEIEEPKVCPACGAHLVHEG
CCHHHHHHHHHHHCHHHEEECCCCHHHHHHHHCCCCCCCCHHCCCCCCCHHHHHHHHHCC
VHIYCENTLGCKPQMVKTIVHFAGREAMNIAGFSEKTAEQLFEKLDIRDISDLYKLEYEK
EEEEECCCCCCCHHHHHHHHHHHCCCHHEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
LLDLDKFGPKKAQNLLDAIEKSKDCTLEAFLYSLGIPNVGVKTAKDLVKRFESLENLEKA
HHCHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
TFEELVSVQDVGDIVARSIIEFFKEERTLKVINELLSLGVNPHYEKKEVLESPFMGKTVV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCEEE
VTGTLENYSRTSIKEKLESLGAKVSGSVSKKTDFVIAGEAAGSKYDKAKSLGVTILSEEE
EEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHCCEEEEEHHH
FENMI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA