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The map label for this gene is ogt [H]

Identifier: 182682591

GI number: 182682591

Start: 2338979

End: 2339497

Strand: Direct

Name: ogt [H]

Synonym: XfasM23_2079

Alternate gene names: 182682591

Gene position: 2338979-2339497 (Clockwise)

Preceding gene: 182682589

Following gene: 182682592

Centisome position: 92.24

GC content: 51.83

Gene sequence:

>519_bases
ATGTCGCAACTGTGCTATGACATCTTTGATAGTCCGATTGGAATACTGACAATTGTCGCCGATACAGATGCACTATGTCG
CGTGATGCTCCCAAGCAACGATCACACAACAAAAGAGCACGAATGCTGGCACTATGCTCCGAGTACAACACTATTAAAGC
AAGCACGCACCCAGTTACTCGACTATTTGCACGGCGAGCTCCACACATTCTCGCTACCGTTACGACCACAGGGCACACCA
TTCCAATGTGCGGTATGGAAGGCACTAGCCCACATCTCATTCGGCCAGACTTGGAGTTATGCGAAATTAGCTGCTCACAT
TGGGCGACCAAGTGCAAGCCGTGCCGTAGGAGCCGCAAACGCTCGCAACCCACTGCCCATCGTATTACCTTGCCACCGTG
TGATTGGCAGCAATGGCGCGCTCACCGGCTTCAACGGCGGACTACATATCAAACGCTCCCTGCTGTACCTGGAAGGTACC
AAACCAACTCATACGTGCAAGACAATCATGACATCGTGA

Upstream 100 bases:

>100_bases
ACTGCACGTACTATCACGCGTCCTGGAACTTGATGGCCGTAGAAGATTTTTATTTTGCTAAATCGATACTACCTAGCCGT
GACCCTAAAGAGGCCTCATG

Downstream 100 bases:

>100_bases
CATCACTACTACTGCTGATATTGCTGCAATGATGATCGCACGCACGTTATCTATATTCATCGGCACCCTGCTATTGGCGA
CCTGCTCGATTCCTACTCGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MSQLCYDIFDSPIGILTIVADTDALCRVMLPSNDHTTKEHECWHYAPSTTLLKQARTQLLDYLHGELHTFSLPLRPQGTP
FQCAVWKALAHISFGQTWSYAKLAAHIGRPSASRAVGAANARNPLPIVLPCHRVIGSNGALTGFNGGLHIKRSLLYLEGT
KPTHTCKTIMTS

Sequences:

>Translated_172_residues
MSQLCYDIFDSPIGILTIVADTDALCRVMLPSNDHTTKEHECWHYAPSTTLLKQARTQLLDYLHGELHTFSLPLRPQGTP
FQCAVWKALAHISFGQTWSYAKLAAHIGRPSASRAVGAANARNPLPIVLPCHRVIGSNGALTGFNGGLHIKRSLLYLEGT
KPTHTCKTIMTS
>Mature_171_residues
SQLCYDIFDSPIGILTIVADTDALCRVMLPSNDHTTKEHECWHYAPSTTLLKQARTQLLDYLHGELHTFSLPLRPQGTPF
QCAVWKALAHISFGQTWSYAKLAAHIGRPSASRAVGAANARNPLPIVLPCHRVIGSNGALTGFNGGLHIKRSLLYLEGTK
PTHTCKTIMTS

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=163, Percent_Identity=34.9693251533742, Blast_Score=81, Evalue=6e-16,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=52.4271844660194, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788542, Length=159, Percent_Identity=33.9622641509434, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=49.3975903614458, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=83, Percent_Identity=49.3975903614458, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=78, Percent_Identity=42.3076923076923, Blast_Score=76, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137554, Length=91, Percent_Identity=45.0549450549451, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18861; Mature: 18730

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLCYDIFDSPIGILTIVADTDALCRVMLPSNDHTTKEHECWHYAPSTTLLKQARTQLL
CCCHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHH
DYLHGELHTFSLPLRPQGTPFQCAVWKALAHISFGQTWSYAKLAAHIGRPSASRAVGAAN
HHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHCCCCCC
ARNPLPIVLPCHRVIGSNGALTGFNGGLHIKRSLLYLEGTKPTHTCKTIMTS
CCCCCEEEEECHHHHCCCCCEECCCCCEEEEEEEEEEECCCCCHHHHHEECC
>Mature Secondary Structure 
SQLCYDIFDSPIGILTIVADTDALCRVMLPSNDHTTKEHECWHYAPSTTLLKQARTQLL
CCHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHH
DYLHGELHTFSLPLRPQGTPFQCAVWKALAHISFGQTWSYAKLAAHIGRPSASRAVGAAN
HHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHCCCCCC
ARNPLPIVLPCHRVIGSNGALTGFNGGLHIKRSLLYLEGTKPTHTCKTIMTS
CCCCCEEEEECHHHHCCCCCEECCCCCEEEEEEEEEEECCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]