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The map label for this gene is leuB [H]

Identifier: 182682589

GI number: 182682589

Start: 2336416

End: 2337423

Strand: Direct

Name: leuB [H]

Synonym: XfasM23_2077

Alternate gene names: 182682589

Gene position: 2336416-2337423 (Clockwise)

Preceding gene: 182682588

Following gene: 182682591

Centisome position: 92.14

GC content: 51.39

Gene sequence:

>1008_bases
ATGACGCAAACAATTACGGTGATCCGCGGTGATGGTATCGGCCCGGAAATCATGGATGCCACCCTGTCGGTATTAAACGC
GTTACAAGCGAATCTGAATTACGAATTCGCAGATGCAGGCTTGAGCGCACTTGAAAAACACGGAGACTTGCTACCGGAAT
CCACCCTGAATTCAATCAGCAAGAACAAAATCGCGCTAAAAAGCCCGCTCACAACCCCGGTAGGTGAAGGTTTCAGCTCG
ATCAATGTCGCCCTGCGACGTAAATTCGACTTATACGCCAACGTTCGCCCCGCAAAATCTTTCCCAAATACCAAGTCACG
CTTTAGCGATAACGTAGACCTGATCACCGTACGGGAAAATACCGAGGGTGCCTACCTGAGCGAGGGTCAAACCATCTCTC
CTGACGGAGAAGTTGCCACTTCAAGCGCACGAGTGACCCGTAAAGGGTCGGAACGTATCGTCCGTTACGCATTCGACTTA
GCCCGAACAACGGGCCGCAAAAAGGTCACCGCAGTACACAAAGCCAACATCATTAAATCAACCTCTGGACTATTTCTGAA
AACCGCCCGAGACGTCGCAAGCCAGTACCCAGAAATCGATTTCCAAGAAATGATCGTCGACAACACTTGCATGCAACTTG
TAATGCGCCCAGAACAATTCGACATCATTGTCACCACAAACTTGTTCGGTGACATCCTCTCAGATTTGTGCGCAGGCTTG
GTCGGCGGTTTAGGCTTGGCTCCGGGCGCCAACATTGGTAATGAACAAGCCATCTTCGAAGCCGTACATGGCTCGGCTCC
AGACATCGCTGGACAAGGTAAAGCCAATCCATGCGCGCTGCTGCTAGGTGCTGTACAAATGCTGGATCATATCGGTCAAC
CACAAAACGCCGAACGCCTACGCAACGCAATCGTGGCCACACTGAATGCCAAAGATTCGCTGACTCCCGATCTGGGGGGT
AACGGCAACACCATGGGATTCGCCAAGGCAATTGCAAGCAGACTCTAA

Upstream 100 bases:

>100_bases
CATCTATAGAATTCAGGCATAATACTTCGGAAAAACCCTAGCATCCGCACTTTGGACAGCTAGCTTGCGCTCCCCCTTTT
CAGTTCGGGATTCAACTCTC

Downstream 100 bases:

>100_bases
AACAACGCGTTAGTAACCTCAGATCCAGTGAAGGAGAAAAACTGATCCAATGGTTAGCAATACCTCAACGGTTATTTACT
GTCACACAAACGGACCAATG

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MTQTITVIRGDGIGPEIMDATLSVLNALQANLNYEFADAGLSALEKHGDLLPESTLNSISKNKIALKSPLTTPVGEGFSS
INVALRRKFDLYANVRPAKSFPNTKSRFSDNVDLITVRENTEGAYLSEGQTISPDGEVATSSARVTRKGSERIVRYAFDL
ARTTGRKKVTAVHKANIIKSTSGLFLKTARDVASQYPEIDFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
VGGLGLAPGANIGNEQAIFEAVHGSAPDIAGQGKANPCALLLGAVQMLDHIGQPQNAERLRNAIVATLNAKDSLTPDLGG
NGNTMGFAKAIASRL

Sequences:

>Translated_335_residues
MTQTITVIRGDGIGPEIMDATLSVLNALQANLNYEFADAGLSALEKHGDLLPESTLNSISKNKIALKSPLTTPVGEGFSS
INVALRRKFDLYANVRPAKSFPNTKSRFSDNVDLITVRENTEGAYLSEGQTISPDGEVATSSARVTRKGSERIVRYAFDL
ARTTGRKKVTAVHKANIIKSTSGLFLKTARDVASQYPEIDFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
VGGLGLAPGANIGNEQAIFEAVHGSAPDIAGQGKANPCALLLGAVQMLDHIGQPQNAERLRNAIVATLNAKDSLTPDLGG
NGNTMGFAKAIASRL
>Mature_334_residues
TQTITVIRGDGIGPEIMDATLSVLNALQANLNYEFADAGLSALEKHGDLLPESTLNSISKNKIALKSPLTTPVGEGFSSI
NVALRRKFDLYANVRPAKSFPNTKSRFSDNVDLITVRENTEGAYLSEGQTISPDGEVATSSARVTRKGSERIVRYAFDLA
RTTGRKKVTAVHKANIIKSTSGLFLKTARDVASQYPEIDFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGLV
GGLGLAPGANIGNEQAIFEAVHGSAPDIAGQGKANPCALLLGAVQMLDHIGQPQNAERLRNAIVATLNAKDSLTPDLGGN
GNTMGFAKAIASRL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=337, Percent_Identity=47.7744807121662, Blast_Score=305, Evalue=3e-83,
Organism=Homo sapiens, GI4758582, Length=326, Percent_Identity=44.478527607362, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI28178838, Length=318, Percent_Identity=43.7106918238994, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI28178821, Length=337, Percent_Identity=41.839762611276, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI28178816, Length=337, Percent_Identity=41.5430267062315, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI28178819, Length=193, Percent_Identity=45.5958549222798, Blast_Score=167, Evalue=1e-41,
Organism=Escherichia coli, GI1788101, Length=353, Percent_Identity=34.8441926345609, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1787381, Length=359, Percent_Identity=34.2618384401114, Blast_Score=197, Evalue=6e-52,
Organism=Escherichia coli, GI87081683, Length=308, Percent_Identity=36.038961038961, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI71986051, Length=334, Percent_Identity=44.9101796407186, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI25144293, Length=334, Percent_Identity=41.3173652694611, Blast_Score=243, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI17550882, Length=338, Percent_Identity=41.1242603550296, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17505779, Length=321, Percent_Identity=40.1869158878505, Blast_Score=228, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6324709, Length=337, Percent_Identity=44.213649851632, Blast_Score=286, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6324291, Length=335, Percent_Identity=40, Blast_Score=231, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=38.9204545454545, Blast_Score=216, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6319830, Length=360, Percent_Identity=29.7222222222222, Blast_Score=132, Evalue=8e-32,
Organism=Drosophila melanogaster, GI24643268, Length=334, Percent_Identity=45.8083832335329, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=45.8083832335329, Blast_Score=302, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24661184, Length=340, Percent_Identity=41.4705882352941, Blast_Score=271, Evalue=4e-73,
Organism=Drosophila melanogaster, GI161078637, Length=333, Percent_Identity=37.8378378378378, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI161078633, Length=333, Percent_Identity=37.8378378378378, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24650122, Length=333, Percent_Identity=37.8378378378378, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI161078635, Length=333, Percent_Identity=37.8378378378378, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI161078639, Length=315, Percent_Identity=39.3650793650794, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI281362242, Length=334, Percent_Identity=42.2155688622754, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI24648872, Length=334, Percent_Identity=42.2155688622754, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI20130355, Length=335, Percent_Identity=28.6567164179104, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI45550436, Length=234, Percent_Identity=25.2136752136752, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 35704; Mature: 35573

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTITVIRGDGIGPEIMDATLSVLNALQANLNYEFADAGLSALEKHGDLLPESTLNSIS
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
KNKIALKSPLTTPVGEGFSSINVALRRKFDLYANVRPAKSFPNTKSRFSDNVDLITVREN
CCCEEECCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEEEC
TEGAYLSEGQTISPDGEVATSSARVTRKGSERIVRYAFDLARTTGRKKVTAVHKANIIKS
CCCCEECCCCEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TSGLFLKTARDVASQYPEIDFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
VGGLGLAPGANIGNEQAIFEAVHGSAPDIAGQGKANPCALLLGAVQMLDHIGQPQNAERL
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RNAIVATLNAKDSLTPDLGGNGNTMGFAKAIASRL
HHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
TQTITVIRGDGIGPEIMDATLSVLNALQANLNYEFADAGLSALEKHGDLLPESTLNSIS
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
KNKIALKSPLTTPVGEGFSSINVALRRKFDLYANVRPAKSFPNTKSRFSDNVDLITVREN
CCCEEECCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEEEC
TEGAYLSEGQTISPDGEVATSSARVTRKGSERIVRYAFDLARTTGRKKVTAVHKANIIKS
CCCCEECCCCEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
TSGLFLKTARDVASQYPEIDFQEMIVDNTCMQLVMRPEQFDIIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHH
VGGLGLAPGANIGNEQAIFEAVHGSAPDIAGQGKANPCALLLGAVQMLDHIGQPQNAERL
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHH
RNAIVATLNAKDSLTPDLGGNGNTMGFAKAIASRL
HHHHEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]