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The map label for this gene is phoB [H]

Identifier: 182682586

GI number: 182682586

Start: 2332875

End: 2333570

Strand: Reverse

Name: phoB [H]

Synonym: XfasM23_2074

Alternate gene names: 182682586

Gene position: 2333570-2332875 (Counterclockwise)

Preceding gene: 182682587

Following gene: 182682585

Centisome position: 92.03

GC content: 53.02

Gene sequence:

>696_bases
GTGCAAAAACGCGTCTTGATTGTCGATGATGAGGTGGCGATTCGTGACATGGTTGCTTTCGCTTTGCGTAAGGGTGAATT
TGAATCCTTGCAAGCTAGTGATGCGCGCGAGGCTCAGGCCGTTATCGCAGATTGCGTTCCGGATTTGATTTTGTTGGATT
GGATGCTGCCTGGTACCAGTGGCCTGGAGTTGACACGTCGTTGGCGTAAAGCGTCGCTGACCCGTGAAATACCTATCATC
ATGCTGACCGCGCGTAGTGAGGAGAACGATCGGGTTGGTGGTTTGGAAGCGGGGGTGGATGATTATATGGTCAAGCCGTT
CTCGGCGCGCGAACTGTTGGCGCGCATCCGCGCGGTGATGCGCCGTACTCGTGATGATGAGGAGGATGGTAGTGTTGCCA
TTGGAAAATTACGCATTGATGGTGCTGCGCACCGTGTGTATGCCGGTCATGTGCAAGTGCCAATTGGTCCGACTGAATAC
CGCTTACTGCATTTTTTCATGACACATTCGGAGCGCGTGTACTCGCGTACTCAACTTTTAGATCATGTTTGGGGTAGCGG
CGTATATATAGAGGAGCGCACGATTGATGTGCATATCCGCCGTTTGCGTAGAACGTTGGAGCCATTCGGCCTTGACGATA
TGGTGCAGACGGTACGTAGTGCCGGTTATCGCTTCTCCGGGGCGATCCAGATCTGA

Upstream 100 bases:

>100_bases
CCGGATTTGGATCGATGATCTCTGTGTGTCATATCAGCCATATTTTTGTCATGAATTTGTAGTTTACTGGCGAACATCCT
AAGTAAGGCGGTGTCTGAGC

Downstream 100 bases:

>100_bases
ATATTCCATCGAATGCCGTGAGTATCGTCTCCACACTCCACCGTATTCGGTTCGCTTGGTTCAAGACCCTGGGAGTGTTG
GCGCTGATTTTGTTCGCTGC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQKRVLIVDDEVAIRDMVAFALRKGEFESLQASDAREAQAVIADCVPDLILLDWMLPGTSGLELTRRWRKASLTREIPII
MLTARSEENDRVGGLEAGVDDYMVKPFSARELLARIRAVMRRTRDDEEDGSVAIGKLRIDGAAHRVYAGHVQVPIGPTEY
RLLHFFMTHSERVYSRTQLLDHVWGSGVYIEERTIDVHIRRLRRTLEPFGLDDMVQTVRSAGYRFSGAIQI

Sequences:

>Translated_231_residues
MQKRVLIVDDEVAIRDMVAFALRKGEFESLQASDAREAQAVIADCVPDLILLDWMLPGTSGLELTRRWRKASLTREIPII
MLTARSEENDRVGGLEAGVDDYMVKPFSARELLARIRAVMRRTRDDEEDGSVAIGKLRIDGAAHRVYAGHVQVPIGPTEY
RLLHFFMTHSERVYSRTQLLDHVWGSGVYIEERTIDVHIRRLRRTLEPFGLDDMVQTVRSAGYRFSGAIQI
>Mature_231_residues
MQKRVLIVDDEVAIRDMVAFALRKGEFESLQASDAREAQAVIADCVPDLILLDWMLPGTSGLELTRRWRKASLTREIPII
MLTARSEENDRVGGLEAGVDDYMVKPFSARELLARIRAVMRRTRDDEEDGSVAIGKLRIDGAAHRVYAGHVQVPIGPTEY
RLLHFFMTHSERVYSRTQLLDHVWGSGVYIEERTIDVHIRRLRRTLEPFGLDDMVQTVRSAGYRFSGAIQI

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=56.1946902654867, Blast_Score=270, Evalue=6e-74,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=37.6106194690265, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=35.8078602620087, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1790860, Length=234, Percent_Identity=35.8974358974359, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=32.7354260089686, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786911, Length=204, Percent_Identity=36.2745098039216, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=32.2727272727273, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787375, Length=222, Percent_Identity=30.1801801801802, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=29.957805907173, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=30.4545454545455, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=30, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI2367329, Length=211, Percent_Identity=35.0710900473934, Blast_Score=100, Evalue=6e-23,
Organism=Escherichia coli, GI1787229, Length=237, Percent_Identity=30.8016877637131, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788550, Length=110, Percent_Identity=36.3636363636364, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26308; Mature: 26308

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKRVLIVDDEVAIRDMVAFALRKGEFESLQASDAREAQAVIADCVPDLILLDWMLPGTS
CCCCEEEECCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GLELTRRWRKASLTREIPIIMLTARSEENDRVGGLEAGVDDYMVKPFSARELLARIRAVM
CHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
RRTRDDEEDGSVAIGKLRIDGAAHRVYAGHVQVPIGPTEYRLLHFFMTHSERVYSRTQLL
HHCCCCCCCCCEEEEEEEECCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DHVWGSGVYIEERTIDVHIRRLRRTLEPFGLDDMVQTVRSAGYRFSGAIQI
HHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEC
>Mature Secondary Structure
MQKRVLIVDDEVAIRDMVAFALRKGEFESLQASDAREAQAVIADCVPDLILLDWMLPGTS
CCCCEEEECCCHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GLELTRRWRKASLTREIPIIMLTARSEENDRVGGLEAGVDDYMVKPFSARELLARIRAVM
CHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHH
RRTRDDEEDGSVAIGKLRIDGAAHRVYAGHVQVPIGPTEYRLLHFFMTHSERVYSRTQLL
HHCCCCCCCCCEEEEEEEECCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DHVWGSGVYIEERTIDVHIRRLRRTLEPFGLDDMVQTVRSAGYRFSGAIQI
HHHCCCCEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]