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The map label for this gene is tsf

Identifier: 182682577

GI number: 182682577

Start: 2317226

End: 2318104

Strand: Reverse

Name: tsf

Synonym: XfasM23_2065

Alternate gene names: 182682577

Gene position: 2318104-2317226 (Counterclockwise)

Preceding gene: 182682578

Following gene: 182682574

Centisome position: 91.42

GC content: 49.83

Gene sequence:

>879_bases
ATGGAAATCACTGCATCCCTGGTCAAGGAACTGCGCGAGCGCACCGGCGTAGGCATGATGGAGTGCAAAAAAGCGCTGAG
TGAAAATGCAGGCAATATTGATGCTTCTGTCGAATGGCTGCGTAAATCCGGTTTGGTAAAGGCCGATAAAAAGGCTGGTC
GTATTGCTGCTGAGGGCCGCATTGTGGTAGTTCATGATGGCTGTAAGGCTGTCCTGGTCGAGATCAATTCGGAGACCGAT
TTTGTTGCCAAAGACAGTCATTTTCTGGCATTTGCCGAGGCTGTTGCGCAGGCTGCGTTAGTTGCTGGGGCGGCTGATGT
TGAAGCGCTAAAGCATGTCAAGCTTCCGTCTGGAGAGACTGTTGAGGAAACCCGCGCTGCGGTGATTGCCAAGATTGGCG
AGAATGTGCGGGTGCGTCGTCTAGCGCGGATTGATAGTGCAAATAATGTGGCCGCATACGTACATGGTGGCCGTATCGGT
GTATTGGTTGAAGTCAAGGGAGGCGATGTTGAGTTGGCTCGCGGCATTGCAATGCATGTAGCAGCGATGAACCCCCCATA
TAACAAGGTTGCTGATGTGTCGGCTGAATTTCTGGAAAAGGAAAAGGAGATCGAGCTGAGTAAGATGTCTGAGAAAGACA
AATCTAAGCCTGCCGATATTTTGGAGAAAATTATCAGTGGGAAGATCAACAAAATAGTTAAGGAAGTGACTTTGTATGGT
CAGCCGTATGTGCTTAATCCCGATCAAAGTGTCGAGCAAGTTGTGAAGGCCGCAGGTGCTGATGTGATTGGTTTCCAGCG
CATGGAGGTTGGCGAAGGGATTGAAAAGATCGTCGAAGACTATGCTTCTGAAGTGATGAAGCAGGCCGGCCTTTCTTGA

Upstream 100 bases:

>100_bases
CGATTACAGCATTTCATCTACATAGGGACGGGCGGATTCTCGCCTGTCCTCCCCGGCGATCATTGCTCGCCTCCGGCCTT
TGTATATACGAGGTAATTCT

Downstream 100 bases:

>100_bases
TTTCTCCCTAGGTCGTGTGTTTGTTTGAGGAACCGTGGTACACCCCTGCTGTGTGGTCGCGTGTTCGCCACGGTTGTTTT
GTCAGGCAGCGTCTTAGAGC

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEITASLVKELRERTGVGMMECKKALSENAGNIDASVEWLRKSGLVKADKKAGRIAAEGRIVVVHDGCKAVLVEINSETD
FVAKDSHFLAFAEAVAQAALVAGAADVEALKHVKLPSGETVEETRAAVIAKIGENVRVRRLARIDSANNVAAYVHGGRIG
VLVEVKGGDVELARGIAMHVAAMNPPYNKVADVSAEFLEKEKEIELSKMSEKDKSKPADILEKIISGKINKIVKEVTLYG
QPYVLNPDQSVEQVVKAAGADVIGFQRMEVGEGIEKIVEDYASEVMKQAGLS

Sequences:

>Translated_292_residues
MEITASLVKELRERTGVGMMECKKALSENAGNIDASVEWLRKSGLVKADKKAGRIAAEGRIVVVHDGCKAVLVEINSETD
FVAKDSHFLAFAEAVAQAALVAGAADVEALKHVKLPSGETVEETRAAVIAKIGENVRVRRLARIDSANNVAAYVHGGRIG
VLVEVKGGDVELARGIAMHVAAMNPPYNKVADVSAEFLEKEKEIELSKMSEKDKSKPADILEKIISGKINKIVKEVTLYG
QPYVLNPDQSVEQVVKAAGADVIGFQRMEVGEGIEKIVEDYASEVMKQAGLS
>Mature_292_residues
MEITASLVKELRERTGVGMMECKKALSENAGNIDASVEWLRKSGLVKADKKAGRIAAEGRIVVVHDGCKAVLVEINSETD
FVAKDSHFLAFAEAVAQAALVAGAADVEALKHVKLPSGETVEETRAAVIAKIGENVRVRRLARIDSANNVAAYVHGGRIG
VLVEVKGGDVELARGIAMHVAAMNPPYNKVADVSAEFLEKEKEIELSKMSEKDKSKPADILEKIISGKINKIVKEVTLYG
QPYVLNPDQSVEQVVKAAGADVIGFQRMEVGEGIEKIVEDYASEVMKQAGLS

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=304, Percent_Identity=27.3026315789474, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI291084500, Length=321, Percent_Identity=26.1682242990654, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI291084502, Length=99, Percent_Identity=37.3737373737374, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI291084498, Length=99, Percent_Identity=37.3737373737374, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786366, Length=284, Percent_Identity=50.7042253521127, Blast_Score=265, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17561440, Length=294, Percent_Identity=25.5102040816327, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19921466, Length=311, Percent_Identity=27.6527331189711, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 31347; Mature: 31347

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00092 N6_MTASE ; PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITASLVKELRERTGVGMMECKKALSENAGNIDASVEWLRKSGLVKADKKAGRIAAEGR
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCE
IVVVHDGCKAVLVEINSETDFVAKDSHFLAFAEAVAQAALVAGAADVEALKHVKLPSGET
EEEEECCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC
VEETRAAVIAKIGENVRVRRLARIDSANNVAAYVHGGRIGVLVEVKGGDVELARGIAMHV
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCCHHHHHHHHHH
AAMNPPYNKVADVSAEFLEKEKEIELSKMSEKDKSKPADILEKIISGKINKIVKEVTLYG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
QPYVLNPDQSVEQVVKAAGADVIGFQRMEVGEGIEKIVEDYASEVMKQAGLS
CCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEITASLVKELRERTGVGMMECKKALSENAGNIDASVEWLRKSGLVKADKKAGRIAAEGR
CCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCE
IVVVHDGCKAVLVEINSETDFVAKDSHFLAFAEAVAQAALVAGAADVEALKHVKLPSGET
EEEEECCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC
VEETRAAVIAKIGENVRVRRLARIDSANNVAAYVHGGRIGVLVEVKGGDVELARGIAMHV
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCEEEEEEEECCCCCHHHHHHHHHH
AAMNPPYNKVADVSAEFLEKEKEIELSKMSEKDKSKPADILEKIISGKINKIVKEVTLYG
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
QPYVLNPDQSVEQVVKAAGADVIGFQRMEVGEGIEKIVEDYASEVMKQAGLS
CCEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]