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The map label for this gene is ispG

Identifier: 182682574

GI number: 182682574

Start: 2313696

End: 2314961

Strand: Reverse

Name: ispG

Synonym: XfasM23_2062

Alternate gene names: 182682574

Gene position: 2314961-2313696 (Counterclockwise)

Preceding gene: 182682577

Following gene: 182682573

Centisome position: 91.3

GC content: 55.37

Gene sequence:

>1266_bases
ATGCACGACATCGTGACTCCTCAAGTTGCTTCTGATTCCGCCGTACCTTGGCCGCGGCGCCTTACGCAGGCAGTCAATAT
TGGTCACCTGACCGTTGGAGGGGGACACCCAGTAGTGGTGCAGTCGATGACCAACACGGATACAGCCGACGTTGTTGCTA
GTACCAAGCAGGTTGCCGAATTGTGGCGTGCCGGTTCTGAGATGGTGAGGTTGACCGTCAACAATGCCCAATCGGCTGCT
GCAATCCCGCGTATTGCCGAACGCTTGGCAATGATGGGCATTGAAGTCCCCTTGATCGGTGATTTTCATTACAACGGTCA
TCAATTATTGGCTGACGAACCGGCGTGTGCGGAAGTGCTGGCCAAGTACCGGATCAATCCTGGTAATGTCGGTTTTGGAA
AGAAGCGGGATTTGCAGTTCGGCCAATTGATTGAATGCGCGATCCGCTATGGCAAACCCATTAGGATTGGCGCCAATTGG
GGATCATTGGATCAGATGTTGGCGGCGCAATTGATGGATGAGAACAATCGGCGCAAGCAGCCATGGGATGCCGCTCAGGT
GTTGCGTGAGGTATTGATTTTTTCGGCGGTAGGCTCTGCCGAGCGCGCTGTTGAGTTGGGGTTGCCGCGTGATCGCATTG
TGCTCTCGGCCAAGGTGTCTGGTGTGCAGGAGCTGATTGCGGTATACCGTGATATGGCAGCGCGCTGTGATTTTGCCTTG
CATCTTGGCCTCACTGAGGCTGGTATTGGGAGTAAAGGGATCGTTGCGTCCTCAGCGGCGCTGGCAGTATTACTGCAAGA
AGGGATCGGAGATACGATCCGCATTTCGTTGACCCCTGAGCCGGGGCAATCGCGTACCCAAGAGGTGATTGTGGCCCAGG
AGTTGTTGCAGACAACTGGTCATCGTGCCTTTACTCCGATGGTCACTGCTTGCCCGGGGTGTGGGCGTACCACTTCGGAA
TTCTTCCAGGAACTCGCCAAGACGGTGCAGCAGCACGTGCGCGACAAGATGCAGGTGTGGAAAATCACTCACCCTGGTGC
GGAAACTATGACCTTGGCCGTCATGGGTTGTGTGGTGAACGGGCCGGGGGAATCGCGTCACGCCAACATTGGTATTTCGC
TGCCTGGCACGGGAGAGATGCCGGTTGCGCCGGTGTTTATTGATGGTGAGAAGTCGGTAACCTTGCGTGGCGGAAACATT
GCCCAGGACTTCATCACTTTAGTTGACGATTACGTAGAACGCAGGTATGCCCGTTTCCCCGACTGA

Upstream 100 bases:

>100_bases
CGATTGCGGTCAGATGTGTTGACGATATGTATGTGGGTGTCGATGTTGATCCAGCACAAAGATGTGAGTGATTCAGTGGC
TCAAGGTTTAAACTACGCAT

Downstream 100 bases:

>100_bases
TGCGTTTGTCGGCTTCTGTCGTTGGCGACGCGCTCATGTGCGGCATCACCAGTTGCTGTGCTTAGGGGTGTTGCTGCCTC
TTGGTCTGTGCGTTGTGTTG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MHDIVTPQVASDSAVPWPRRLTQAVNIGHLTVGGGHPVVVQSMTNTDTADVVASTKQVAELWRAGSEMVRLTVNNAQSAA
AIPRIAERLAMMGIEVPLIGDFHYNGHQLLADEPACAEVLAKYRINPGNVGFGKKRDLQFGQLIECAIRYGKPIRIGANW
GSLDQMLAAQLMDENNRRKQPWDAAQVLREVLIFSAVGSAERAVELGLPRDRIVLSAKVSGVQELIAVYRDMAARCDFAL
HLGLTEAGIGSKGIVASSAALAVLLQEGIGDTIRISLTPEPGQSRTQEVIVAQELLQTTGHRAFTPMVTACPGCGRTTSE
FFQELAKTVQQHVRDKMQVWKITHPGAETMTLAVMGCVVNGPGESRHANIGISLPGTGEMPVAPVFIDGEKSVTLRGGNI
AQDFITLVDDYVERRYARFPD

Sequences:

>Translated_421_residues
MHDIVTPQVASDSAVPWPRRLTQAVNIGHLTVGGGHPVVVQSMTNTDTADVVASTKQVAELWRAGSEMVRLTVNNAQSAA
AIPRIAERLAMMGIEVPLIGDFHYNGHQLLADEPACAEVLAKYRINPGNVGFGKKRDLQFGQLIECAIRYGKPIRIGANW
GSLDQMLAAQLMDENNRRKQPWDAAQVLREVLIFSAVGSAERAVELGLPRDRIVLSAKVSGVQELIAVYRDMAARCDFAL
HLGLTEAGIGSKGIVASSAALAVLLQEGIGDTIRISLTPEPGQSRTQEVIVAQELLQTTGHRAFTPMVTACPGCGRTTSE
FFQELAKTVQQHVRDKMQVWKITHPGAETMTLAVMGCVVNGPGESRHANIGISLPGTGEMPVAPVFIDGEKSVTLRGGNI
AQDFITLVDDYVERRYARFPD
>Mature_421_residues
MHDIVTPQVASDSAVPWPRRLTQAVNIGHLTVGGGHPVVVQSMTNTDTADVVASTKQVAELWRAGSEMVRLTVNNAQSAA
AIPRIAERLAMMGIEVPLIGDFHYNGHQLLADEPACAEVLAKYRINPGNVGFGKKRDLQFGQLIECAIRYGKPIRIGANW
GSLDQMLAAQLMDENNRRKQPWDAAQVLREVLIFSAVGSAERAVELGLPRDRIVLSAKVSGVQELIAVYRDMAARCDFAL
HLGLTEAGIGSKGIVASSAALAVLLQEGIGDTIRISLTPEPGQSRTQEVIVAQELLQTTGHRAFTPMVTACPGCGRTTSE
FFQELAKTVQQHVRDKMQVWKITHPGAETMTLAVMGCVVNGPGESRHANIGISLPGTGEMPVAPVFIDGEKSVTLRGGNI
AQDFITLVDDYVERRYARFPD

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=358, Percent_Identity=32.6815642458101, Blast_Score=168, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45487; Mature: 45487

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDIVTPQVASDSAVPWPRRLTQAVNIGHLTVGGGHPVVVQSMTNTDTADVVASTKQVAE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LWRAGSEMVRLTVNNAQSAAAIPRIAERLAMMGIEVPLIGDFHYNGHQLLADEPACAEVL
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHH
AKYRINPGNVGFGKKRDLQFGQLIECAIRYGKPIRIGANWGSLDQMLAAQLMDENNRRKQ
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
PWDAAQVLREVLIFSAVGSAERAVELGLPRDRIVLSAKVSGVQELIAVYRDMAARCDFAL
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCEEE
HLGLTEAGIGSKGIVASSAALAVLLQEGIGDTIRISLTPEPGQSRTQEVIVAQELLQTTG
EECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
HRAFTPMVTACPGCGRTTSEFFQELAKTVQQHVRDKMQVWKITHPGAETMTLAVMGCVVN
CCEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHEEEEEEEEC
GPGESRHANIGISLPGTGEMPVAPVFIDGEKSVTLRGGNIAQDFITLVDDYVERRYARFP
CCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
D
C
>Mature Secondary Structure
MHDIVTPQVASDSAVPWPRRLTQAVNIGHLTVGGGHPVVVQSMTNTDTADVVASTKQVAE
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHH
LWRAGSEMVRLTVNNAQSAAAIPRIAERLAMMGIEVPLIGDFHYNGHQLLADEPACAEVL
HHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHH
AKYRINPGNVGFGKKRDLQFGQLIECAIRYGKPIRIGANWGSLDQMLAAQLMDENNRRKQ
HHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
PWDAAQVLREVLIFSAVGSAERAVELGLPRDRIVLSAKVSGVQELIAVYRDMAARCDFAL
CCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCEEE
HLGLTEAGIGSKGIVASSAALAVLLQEGIGDTIRISLTPEPGQSRTQEVIVAQELLQTTG
EECCHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
HRAFTPMVTACPGCGRTTSEFFQELAKTVQQHVRDKMQVWKITHPGAETMTLAVMGCVVN
CCEECCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHEEEEEEEEC
GPGESRHANIGISLPGTGEMPVAPVFIDGEKSVTLRGGNIAQDFITLVDDYVERRYARFP
CCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]