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The map label for this gene is thrS

Identifier: 182682533

GI number: 182682533

Start: 2261362

End: 2263269

Strand: Reverse

Name: thrS

Synonym: XfasM23_2020

Alternate gene names: 182682533

Gene position: 2263269-2261362 (Counterclockwise)

Preceding gene: 182682534

Following gene: 182682532

Centisome position: 89.26

GC content: 50.31

Gene sequence:

>1908_bases
ATGATTACGATTACGCTCCCAGACGGCAGTCGCCGTGAATTTGAAGGTCCCGTTAGCGTTATGCAGGTTGCTCATGCAAT
CGGTCCGGGCTTGGCCAAGGCCACCATTGCCGGGCAGATTGATGGGGGACACTTAGTGGATGCGAGTGATCTCATTGAGC
ATGATGCTATCCTGCGAATCATCACTCCTGAGGATCAAGAAGCCCTGGAGATTATCCGTCACTCTTGCGCTCACTTGGTT
GGCCATGCGGTCAAACAGTTGTATCCCGAAGCCAAAATGGTTATTGGTCCAGTGATTGCTGATGGTTTTTACTACGATAT
TTACTCCAAGCGTCCGTTCACCCCTGAAGATTTGGCTGCGATTGAGCAGCGTATGGTCGAGTTGATTGCCCAGGACTACG
ATGTGGTCAAACACATCACTGCACGTCACGATGTCGTGCGCTTGTTCAAAGAGCGTGGAGAGGATTACAAACTGCGTCTC
ATCGAAGATATGGGGCCTGAAGTTACTGCGATGGGGATTTATCATCATCAAGAATATGTAGATATGTGCCGTGGCCCGCA
CGTGCCTAATACGCGTTTCTTGAAGGCTTTCAGATTGACACGCATTTCTGGTGCTTACTGGCGTGGTAACACAAAGAATG
AGCAGTTGCAGCGCATCTATGGCACTGCTTGGGCCGACAAGAAGCAACTTGAAGCCTATATGCAACGGTTACAGGATGCT
GAAAAGCGAGATCACCGCAAGATAGGTAAGGCGCAGGATTTATTCCACCTCCAGGAAGAAGGGCCAGGTTTGGTGTTCTG
GCATCCGAAAGGCTGGGTGATCTGGCAGACAATCGAGAATTACATCCGTCGTGTCTATCGCAACAGCGGCTATGGTGAAT
TGCGTTGCCCGCAAATTTTGGATGTGTCGTTGTGGCAAAAGTCTGGTCACTGGGATAATTACAAAGAGAACATGTTTTTT
ACTGATTCGGAGAAGCGTACCTACGCCGTTAAACCTATGAATTGTCCTGGCCATGTTCAAGTGTTCAATCAGGGCTTACA
TAGCTACCGTGATTTGCCGATCCGCTATGGCGAATTCGGTGCTTGCCATCGTAATGAACCATCCGGTGCGTTACATGGTC
TGTTGCGTGTGCGTGGCTTCACGCAGGATGACGGCCACATATTCTGTACCGAGGCACAGATTGAAGCCGAGGTCACGGCA
TTCCACCGTCAAGCGTTGCAGGTCTATGCAGACTTTGGTTTCGAGAATATTCAGATCAAAATTGCCTTGCGTCCAGACAA
GCGCCTAGGGGATTCCCTAAGTTGGGACAAAGCGGAGGCTGCGTTGCGTGCTGCTCTGAGTGCCTGTGAGGTGGAGTGGC
AAGAGCTGCCTGGCGAAGGGGCGTTCTACGGTCCGAAGATCGAATATCATCTGAAAGACGCGATTGGCCGCACTTGGCAG
TTGGGCACGATTCAGGTTGATTTCATGATGCCAGCTCGTCTCGGCGCAGAGTATGTAGACGAGCGCAGCCAGCGCCGTCA
CCCGGTCATGTTGCACCGGGCAATCGTGGGTTCGATGGAACGTTTCATCGGTATTTTGATTGAACACTACGCTGGAATTT
GGCCTACTTGGCTTGCGCCTGTCCAGGTAGTGATCGCTAATATTACTGATGCTCAGTATGAATACGCAGAGCAGGTCCAT
AAAGCCCTTTTAAATCAAGGTTTCCGGGTCAACATTGATTTGCGGAATGAGAAAATCGGTTATAAGATTCGCGAACATAC
ACTGCAACGCGTCCCTTACCTTTTGGTGGTTGGTGATCGTGAAAAGGAGAATGGCACTGTTGCTGTACGCACGTGTTCCA
GAGAGGATTTGGGTGCTATGTCGATTTCCACTTTCGTTGAGCGTTTGCAGACAGAGCAAGTTGTGTGA

Upstream 100 bases:

>100_bases
CATTAGCATCATTGCCTGATCCTGCTTGGCAGAATTCAACAACACTGATGCCTCATCTCAATACTTACATATATCAATCG
CTGACACGCCTTTTATACTC

Downstream 100 bases:

>100_bases
GCCAACCCTGGTTAGTCCAGAGATATGATCTGGACGGCGCCGATTTGATTTCCTTGGAGATTGCAATATCAGTACCTCTG
ACAACAAACAGAACCGTAAG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 635; Mature: 635

Protein sequence:

>635_residues
MITITLPDGSRREFEGPVSVMQVAHAIGPGLAKATIAGQIDGGHLVDASDLIEHDAILRIITPEDQEALEIIRHSCAHLV
GHAVKQLYPEAKMVIGPVIADGFYYDIYSKRPFTPEDLAAIEQRMVELIAQDYDVVKHITARHDVVRLFKERGEDYKLRL
IEDMGPEVTAMGIYHHQEYVDMCRGPHVPNTRFLKAFRLTRISGAYWRGNTKNEQLQRIYGTAWADKKQLEAYMQRLQDA
EKRDHRKIGKAQDLFHLQEEGPGLVFWHPKGWVIWQTIENYIRRVYRNSGYGELRCPQILDVSLWQKSGHWDNYKENMFF
TDSEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRYGEFGACHRNEPSGALHGLLRVRGFTQDDGHIFCTEAQIEAEVTA
FHRQALQVYADFGFENIQIKIALRPDKRLGDSLSWDKAEAALRAALSACEVEWQELPGEGAFYGPKIEYHLKDAIGRTWQ
LGTIQVDFMMPARLGAEYVDERSQRRHPVMLHRAIVGSMERFIGILIEHYAGIWPTWLAPVQVVIANITDAQYEYAEQVH
KALLNQGFRVNIDLRNEKIGYKIREHTLQRVPYLLVVGDREKENGTVAVRTCSREDLGAMSISTFVERLQTEQVV

Sequences:

>Translated_635_residues
MITITLPDGSRREFEGPVSVMQVAHAIGPGLAKATIAGQIDGGHLVDASDLIEHDAILRIITPEDQEALEIIRHSCAHLV
GHAVKQLYPEAKMVIGPVIADGFYYDIYSKRPFTPEDLAAIEQRMVELIAQDYDVVKHITARHDVVRLFKERGEDYKLRL
IEDMGPEVTAMGIYHHQEYVDMCRGPHVPNTRFLKAFRLTRISGAYWRGNTKNEQLQRIYGTAWADKKQLEAYMQRLQDA
EKRDHRKIGKAQDLFHLQEEGPGLVFWHPKGWVIWQTIENYIRRVYRNSGYGELRCPQILDVSLWQKSGHWDNYKENMFF
TDSEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRYGEFGACHRNEPSGALHGLLRVRGFTQDDGHIFCTEAQIEAEVTA
FHRQALQVYADFGFENIQIKIALRPDKRLGDSLSWDKAEAALRAALSACEVEWQELPGEGAFYGPKIEYHLKDAIGRTWQ
LGTIQVDFMMPARLGAEYVDERSQRRHPVMLHRAIVGSMERFIGILIEHYAGIWPTWLAPVQVVIANITDAQYEYAEQVH
KALLNQGFRVNIDLRNEKIGYKIREHTLQRVPYLLVVGDREKENGTVAVRTCSREDLGAMSISTFVERLQTEQVV
>Mature_635_residues
MITITLPDGSRREFEGPVSVMQVAHAIGPGLAKATIAGQIDGGHLVDASDLIEHDAILRIITPEDQEALEIIRHSCAHLV
GHAVKQLYPEAKMVIGPVIADGFYYDIYSKRPFTPEDLAAIEQRMVELIAQDYDVVKHITARHDVVRLFKERGEDYKLRL
IEDMGPEVTAMGIYHHQEYVDMCRGPHVPNTRFLKAFRLTRISGAYWRGNTKNEQLQRIYGTAWADKKQLEAYMQRLQDA
EKRDHRKIGKAQDLFHLQEEGPGLVFWHPKGWVIWQTIENYIRRVYRNSGYGELRCPQILDVSLWQKSGHWDNYKENMFF
TDSEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRYGEFGACHRNEPSGALHGLLRVRGFTQDDGHIFCTEAQIEAEVTA
FHRQALQVYADFGFENIQIKIALRPDKRLGDSLSWDKAEAALRAALSACEVEWQELPGEGAFYGPKIEYHLKDAIGRTWQ
LGTIQVDFMMPARLGAEYVDERSQRRHPVMLHRAIVGSMERFIGILIEHYAGIWPTWLAPVQVVIANITDAQYEYAEQVH
KALLNQGFRVNIDLRNEKIGYKIREHTLQRVPYLLVVGDREKENGTVAVRTCSREDLGAMSISTFVERLQTEQVV

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=633, Percent_Identity=39.1785150078989, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI38570111, Length=635, Percent_Identity=37.6377952755906, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI20070344, Length=651, Percent_Identity=35.7910906298003, Blast_Score=389, Evalue=1e-108,
Organism=Escherichia coli, GI1788013, Length=632, Percent_Identity=59.3354430379747, Blast_Score=803, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=179, Percent_Identity=24.5810055865922, Blast_Score=70, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71984184, Length=639, Percent_Identity=37.5586854460094, Blast_Score=468, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71984192, Length=639, Percent_Identity=37.5586854460094, Blast_Score=468, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322112, Length=630, Percent_Identity=37.4603174603175, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322654, Length=413, Percent_Identity=36.5617433414044, Blast_Score=287, Evalue=3e-78,
Organism=Drosophila melanogaster, GI24583842, Length=634, Percent_Identity=38.3280757097792, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574141, Length=634, Percent_Identity=38.3280757097792, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24583839, Length=634, Percent_Identity=38.3280757097792, Blast_Score=472, Evalue=1e-133,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_XYLF2 (B2I9P8)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830693.1
- ProteinModelPortal:   B2I9P8
- SMR:   B2I9P8
- GeneID:   6202154
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_2020
- HOGENOM:   HBG352811
- OMA:   GFEYEVE
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 72814; Mature: 72814

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITITLPDGSRREFEGPVSVMQVAHAIGPGLAKATIAGQIDGGHLVDASDLIEHDAILRI
CEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHEEEEECCCCCEECHHHHHCCCCEEEE
ITPEDQEALEIIRHSCAHLVGHAVKQLYPEAKMVIGPVIADGFYYDIYSKRPFTPEDLAA
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHH
IEQRMVELIAQDYDVVKHITARHDVVRLFKERGEDYKLRLIEDMGPEVTAMGIYHHQEYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEHHHHHHH
DMCRGPHVPNTRFLKAFRLTRISGAYWRGNTKNEQLQRIYGTAWADKKQLEAYMQRLQDA
HHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EKRDHRKIGKAQDLFHLQEEGPGLVFWHPKGWVIWQTIENYIRRVYRNSGYGELRCPQIL
HHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHH
DVSLWQKSGHWDNYKENMFFTDSEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRYGEFG
HHHHHHCCCCCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCC
ACHRNEPSGALHGLLRVRGFTQDDGHIFCTEAQIEAEVTAFHRQALQVYADFGFENIQIK
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IALRPDKRLGDSLSWDKAEAALRAALSACEVEWQELPGEGAFYGPKIEYHLKDAIGRTWQ
EEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEHHHCCCEEE
LGTIQVDFMMPARLGAEYVDERSQRRHPVMLHRAIVGSMERFIGILIEHYAGIWPTWLAP
EEEEEEEEECCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VQVVIANITDAQYEYAEQVHKALLNQGFRVNIDLRNEKIGYKIREHTLQRVPYLLVVGDR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCC
EKENGTVAVRTCSREDLGAMSISTFVERLQTEQVV
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITITLPDGSRREFEGPVSVMQVAHAIGPGLAKATIAGQIDGGHLVDASDLIEHDAILRI
CEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHEEEEECCCCCEECHHHHHCCCCEEEE
ITPEDQEALEIIRHSCAHLVGHAVKQLYPEAKMVIGPVIADGFYYDIYSKRPFTPEDLAA
ECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCCCCCCHHHHHH
IEQRMVELIAQDYDVVKHITARHDVVRLFKERGEDYKLRLIEDMGPEVTAMGIYHHQEYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEHHHHHHH
DMCRGPHVPNTRFLKAFRLTRISGAYWRGNTKNEQLQRIYGTAWADKKQLEAYMQRLQDA
HHHCCCCCCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EKRDHRKIGKAQDLFHLQEEGPGLVFWHPKGWVIWQTIENYIRRVYRNSGYGELRCPQIL
HHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHH
DVSLWQKSGHWDNYKENMFFTDSEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRYGEFG
HHHHHHCCCCCCCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCC
ACHRNEPSGALHGLLRVRGFTQDDGHIFCTEAQIEAEVTAFHRQALQVYADFGFENIQIK
CCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
IALRPDKRLGDSLSWDKAEAALRAALSACEVEWQELPGEGAFYGPKIEYHLKDAIGRTWQ
EEECCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEHHHCCCEEE
LGTIQVDFMMPARLGAEYVDERSQRRHPVMLHRAIVGSMERFIGILIEHYAGIWPTWLAP
EEEEEEEEECCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VQVVIANITDAQYEYAEQVHKALLNQGFRVNIDLRNEKIGYKIREHTLQRVPYLLVVGDR
HHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCEEEEECCC
EKENGTVAVRTCSREDLGAMSISTFVERLQTEQVV
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA