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The map label for this gene is ddl

Identifier: 182682480

GI number: 182682480

Start: 2206522

End: 2207484

Strand: Reverse

Name: ddl

Synonym: XfasM23_1967

Alternate gene names: 182682480

Gene position: 2207484-2206522 (Counterclockwise)

Preceding gene: 182682481

Following gene: 182682479

Centisome position: 87.06

GC content: 52.44

Gene sequence:

>963_bases
ATGGGTATGAGCATATTTTCACTGTCTCCACGGTATACGGATCCGGCGGTGTTTGGTCGTGTTGCAGTGTTATTAGGGGG
GACGTCTGCTGAGCGTGAGGTGTCTTTGCTTTCAGGTCGTAATGTGTTGGAAGTATTGCGCATGCGCGGTGTTGATGCTC
AGTCGGTAGATGGGGTTCCGGCACTCGCTCAGGCGCTGGTGGAACGGCGTTTTGATCGTGTGTTCAATGTGTTGCACGGT
CATAATGGCGGTGGTGAGGATGGTGTGGTGCAGGGGTTGATGCAGGCTTTTGGTGTGCCGTATACAGGTTCGGATGTGCT
TGGTTCAGCGTTGAGTATGGATAAGGTGCGCACTAAACAGGTTTGGCTTGCGCTGGGTTTGCCGACGCCGCGTTATGCGA
GTTTGAGTGTGTGTGCCACTGCTGTTGAGGTGCGTAAGGCGGTTGAGATGCTAGGTTTTCCGGTGATTATTAAGCCGGCT
AAAGAAGGGTCTAGTGTTGGTGTCAGTCGTGTGTTTGCACTGGAGCATTTGGAGGAGGCGGTTGCTTTAGCGGCGCGTTA
CGAGGGTGAGTTGCTGATGGAGCAGTTGATCGAAGGGGATGAATTGACGGTGTCGATTCTTGATGAAATGGCGTTGCCCT
CAATTCGGATCGTTCCTCAGGGGCAGTGGTATGACTATAACGCGAAGTATCTTGCTGAAGACACGCAGTATGTGTGCCCG
GGTTTGGATGATGTTGCTGAGGCTGAAATCGCTCAACTTGCGTTGGCTGCATTCCATAGTGTTGGTTGTCGTGGTTGGGG
TCGTGTGGATGTGATGCGCGAGCGCGGCAGTGGGCGCTTTTTCTTATTGGAAGTCAATACTGCTCCTGGAATGACGACTC
ACTCGTTAGTGCCAAAGGCAGCGAGTCAGTTGGGTATGGGTTTTGATGATTTGGTGTGGCGGATTCTGGAGCAGACTTTA
TGA

Upstream 100 bases:

>100_bases
TACAGGAGGGGGATTTGCTGTTGATGATGGGTGCTGGCGATATTGGTTCCATCGCGCAGCGGATTGTTCATGATGGTTTT
GTCTTTGGAGAGGTTTAAAT

Downstream 100 bases:

>100_bases
GTTGCTTTGGTATCCGTATGGATGGGAGGCAGGACGGCTGTGCATTTGTTGCTGGAAGTACGGTAATGGTGGTGTCTTGT
GTATCGTCCGCTTGGAGTGT

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MGMSIFSLSPRYTDPAVFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFNVLHG
HNGGGEDGVVQGLMQAFGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPA
KEGSSVGVSRVFALEHLEEAVALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCP
GLDDVAEAEIAQLALAAFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRILEQTL

Sequences:

>Translated_320_residues
MGMSIFSLSPRYTDPAVFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFNVLHG
HNGGGEDGVVQGLMQAFGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPA
KEGSSVGVSRVFALEHLEEAVALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCP
GLDDVAEAEIAQLALAAFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRILEQTL
>Mature_319_residues
GMSIFSLSPRYTDPAVFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVPALAQALVERRFDRVFNVLHGH
NGGGEDGVVQGLMQAFGVPYTGSDVLGSALSMDKVRTKQVWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPAK
EGSSVGVSRVFALEHLEEAVALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCPG
LDDVAEAEIAQLALAAFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKAASQLGMGFDDLVWRILEQTL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=307, Percent_Identity=51.4657980456026, Blast_Score=289, Evalue=2e-79,
Organism=Escherichia coli, GI1786579, Length=354, Percent_Identity=30.7909604519774, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 34613; Mature: 34482

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMSIFSLSPRYTDPAVFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVP
CCCCEEECCCCCCCHHHHHHHHHEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
ALAQALVERRFDRVFNVLHGHNGGGEDGVVQGLMQAFGVPYTGSDVLGSALSMDKVRTKQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPAKEGSSVGVSRVFALEHLEEA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHH
VALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCP
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCC
GLDDVAEAEIAQLALAAFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCEEEEEECCCCCCCHHHHHHHH
ASQLGMGFDDLVWRILEQTL
HHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
GMSIFSLSPRYTDPAVFGRVAVLLGGTSAEREVSLLSGRNVLEVLRMRGVDAQSVDGVP
CCCEEECCCCCCCHHHHHHHHHEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCHH
ALAQALVERRFDRVFNVLHGHNGGGEDGVVQGLMQAFGVPYTGSDVLGSALSMDKVRTKQ
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
VWLALGLPTPRYASLSVCATAVEVRKAVEMLGFPVIIKPAKEGSSVGVSRVFALEHLEEA
EEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHH
VALAARYEGELLMEQLIEGDELTVSILDEMALPSIRIVPQGQWYDYNAKYLAEDTQYVCP
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCC
GLDDVAEAEIAQLALAAFHSVGCRGWGRVDVMRERGSGRFFLLEVNTAPGMTTHSLVPKA
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCEEEEEECCCCCCCHHHHHHHH
ASQLGMGFDDLVWRILEQTL
HHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]