Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 182682463

GI number: 182682463

Start: 2182660

End: 2184063

Strand: Direct

Name: gltX

Synonym: XfasM23_1950

Alternate gene names: 182682463

Gene position: 2182660-2184063 (Clockwise)

Preceding gene: 182682458

Following gene: 182682464

Centisome position: 86.08

GC content: 57.26

Gene sequence:

>1404_bases
ATGACGTGCCGCACCCGCTTTGCCCCCAGCCCCACCGGTTACCTACACATTGGGGGCGCCCGCACCGCACTGTATTGCTG
GCTGGAAGCACGGCGCCGCAACGGGCAATTCCTACTGCGCATCGAAGACACCGACCGCGAACGCAGCACACAAGCCGCGA
TTGATGCCATCCTGCATGCAATGGACTGGCTGGGACTGGACTACGACGAACCCCCTGTCTACCAAACCCAACGCATCGAA
CGCTACAACCAGGTAGCTGCGCGTCTGCTTGCCGAAGGCAAGGCGTACTACGCTTACGACTCCAAGGACACATTGAACGC
CATGCGTGAAGCAGCTCTGCGCACTGGCGAAAAACCACGCTATAACGGCGCTGCACGTGAAGCCAACCTGCCATACCGGG
ATGATCCCAACCGGGTCATCCGCTTCAAAAATCCGCACACCGGCACCGTGGCGTTCGACGATTTGATCAAAGGTCGCATC
CAGATCTCCAACAGCGAACTGGACGACATGGTCATCCTGCGTCCCGATGGCTACCCAACCTACAACTTCGCCGTGGTTGT
GGACGACTGGGACATGAACATCACCGAAGTGATTCGCGGCGACGACCACATCAACAATACGCCACGGCAAATCAACCTCT
ATCACGCCCTGGGCGCACCACTACCCACATTTGCGCATCTACCGATGATCCTGGACGAACAAGGCGCCAAGTTGTCCAAG
CGTACCGGCGCGGCTGATGTGATGCAGTACAGAGATTCTGGTTACCTGCCCCATGCACTGATTAACTATCTGGTACGGCT
GGGCTGGTCACACGGCGACCAAGAGCTATTCAACCGGCAAGCGCTGATCGACCTGTTCCAAATCAACGATGTCAACTCCA
AAGCAGCGCGCCTTGACATGGCAAAACTTGGCTGGGTGAACCAGCACTATCTGAAGACCGATGACCCGGCGACGCTTGCC
CCGCCATTGGTCTGGCATCTGGAACAACGCGGTATTGATGTCAGCGCCGGTCCTGCTCCGACGGACGTGATCCTGGCCTT
ACGGGAACGCGTCCAGACACTGAAAGAAATGGCCGAGAAAGCAGAAATCTGGTACTGCCCATTACAGCGCTATGATGAAA
TAGCCGTTGCCAAGCATCTCAAACCAGGTGCCGAAACAGCCCTACTGCACGCACGTACGCTGTTAGCCGCACTTCCCGCG
TGGACTGTGGACAACGTGGATACCGCCTTGCGCACGACTGCGACAACACTAGAGATCGGCATGGGCAAAGTGGCACAACC
ATTGCGGGTAGCGATCACCGGGACCCAAGTCAGCCCGGACATTGCCTATACCGTTTACCTCACCGGCCGCAACGAAGCCT
TGAAACGCATTGATGCGGCACTGATAAAAATCTCTACGGCTTGA

Upstream 100 bases:

>100_bases
CTTATTCCACAATGTTGCAATGCTGAAAACGCAACACGCCAGGACGCAACACAAGCCGCTCCGTTAGAATTGCAACGATT
TGCAGTCCATTGAGCCTTCC

Downstream 100 bases:

>100_bases
ATCCCACGGAATCTCTATGAGTAACGATCACGGCTGCACCGCCCCCCATCACCACGTACACGACGCACCCGAGTTCATCC
ATGCAGTGGAGCACGCATGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTCRTRFAPSPTGYLHIGGARTALYCWLEARRRNGQFLLRIEDTDRERSTQAAIDAILHAMDWLGLDYDEPPVYQTQRIE
RYNQVAARLLAEGKAYYAYDSKDTLNAMREAALRTGEKPRYNGAAREANLPYRDDPNRVIRFKNPHTGTVAFDDLIKGRI
QISNSELDDMVILRPDGYPTYNFAVVVDDWDMNITEVIRGDDHINNTPRQINLYHALGAPLPTFAHLPMILDEQGAKLSK
RTGAADVMQYRDSGYLPHALINYLVRLGWSHGDQELFNRQALIDLFQINDVNSKAARLDMAKLGWVNQHYLKTDDPATLA
PPLVWHLEQRGIDVSAGPAPTDVILALRERVQTLKEMAEKAEIWYCPLQRYDEIAVAKHLKPGAETALLHARTLLAALPA
WTVDNVDTALRTTATTLEIGMGKVAQPLRVAITGTQVSPDIAYTVYLTGRNEALKRIDAALIKISTA

Sequences:

>Translated_467_residues
MTCRTRFAPSPTGYLHIGGARTALYCWLEARRRNGQFLLRIEDTDRERSTQAAIDAILHAMDWLGLDYDEPPVYQTQRIE
RYNQVAARLLAEGKAYYAYDSKDTLNAMREAALRTGEKPRYNGAAREANLPYRDDPNRVIRFKNPHTGTVAFDDLIKGRI
QISNSELDDMVILRPDGYPTYNFAVVVDDWDMNITEVIRGDDHINNTPRQINLYHALGAPLPTFAHLPMILDEQGAKLSK
RTGAADVMQYRDSGYLPHALINYLVRLGWSHGDQELFNRQALIDLFQINDVNSKAARLDMAKLGWVNQHYLKTDDPATLA
PPLVWHLEQRGIDVSAGPAPTDVILALRERVQTLKEMAEKAEIWYCPLQRYDEIAVAKHLKPGAETALLHARTLLAALPA
WTVDNVDTALRTTATTLEIGMGKVAQPLRVAITGTQVSPDIAYTVYLTGRNEALKRIDAALIKISTA
>Mature_466_residues
TCRTRFAPSPTGYLHIGGARTALYCWLEARRRNGQFLLRIEDTDRERSTQAAIDAILHAMDWLGLDYDEPPVYQTQRIER
YNQVAARLLAEGKAYYAYDSKDTLNAMREAALRTGEKPRYNGAAREANLPYRDDPNRVIRFKNPHTGTVAFDDLIKGRIQ
ISNSELDDMVILRPDGYPTYNFAVVVDDWDMNITEVIRGDDHINNTPRQINLYHALGAPLPTFAHLPMILDEQGAKLSKR
TGAADVMQYRDSGYLPHALINYLVRLGWSHGDQELFNRQALIDLFQINDVNSKAARLDMAKLGWVNQHYLKTDDPATLAP
PLVWHLEQRGIDVSAGPAPTDVILALRERVQTLKEMAEKAEIWYCPLQRYDEIAVAKHLKPGAETALLHARTLLAALPAW
TVDNVDTALRTTATTLEIGMGKVAQPLRVAITGTQVSPDIAYTVYLTGRNEALKRIDAALIKISTA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=31.8548387096774, Blast_Score=213, Evalue=4e-55,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=54.6637744034707, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI226510925, Length=240, Percent_Identity=37.0833333333333, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI71993394, Length=473, Percent_Identity=30.0211416490486, Blast_Score=195, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6324540, Length=484, Percent_Identity=30.9917355371901, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6321192, Length=209, Percent_Identity=25.3588516746411, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=33.3333333333333, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_XYLF2 (B2I994)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830623.1
- ProteinModelPortal:   B2I994
- SMR:   B2I994
- GeneID:   6202591
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1950
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52501; Mature: 52370

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCRTRFAPSPTGYLHIGGARTALYCWLEARRRNGQFLLRIEDTDRERSTQAAIDAILHA
CCCCCCCCCCCCCEEEECCCCEEHEEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHH
MDWLGLDYDEPPVYQTQRIERYNQVAARLLAEGKAYYAYDSKDTLNAMREAALRTGEKPR
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCC
YNGAAREANLPYRDDPNRVIRFKNPHTGTVAFDDLIKGRIQISNSELDDMVILRPDGYPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCCEEHHHHHCCEEEECCCCCCCEEEECCCCCCC
YNFAVVVDDWDMNITEVIRGDDHINNTPRQINLYHALGAPLPTFAHLPMILDEQGAKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHCCEEECCCCCCHHH
RTGAADVMQYRDSGYLPHALINYLVRLGWSHGDQELFNRQALIDLFQINDVNSKAARLDM
HCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHH
AKLGWVNQHYLKTDDPATLAPPLVWHLEQRGIDVSAGPAPTDVILALRERVQTLKEMAEK
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEIWYCPLQRYDEIAVAKHLKPGAETALLHARTLLAALPAWTVDNVDTALRTTATTLEIG
CCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECC
MGKVAQPLRVAITGTQVSPDIAYTVYLTGRNEALKRIDAALIKISTA
CCCCCCCEEEEEECCEECCCEEEEEEEECCHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TCRTRFAPSPTGYLHIGGARTALYCWLEARRRNGQFLLRIEDTDRERSTQAAIDAILHA
CCCCCCCCCCCCEEEECCCCEEHEEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHH
MDWLGLDYDEPPVYQTQRIERYNQVAARLLAEGKAYYAYDSKDTLNAMREAALRTGEKPR
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCC
YNGAAREANLPYRDDPNRVIRFKNPHTGTVAFDDLIKGRIQISNSELDDMVILRPDGYPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCCEEHHHHHCCEEEECCCCCCCEEEECCCCCCC
YNFAVVVDDWDMNITEVIRGDDHINNTPRQINLYHALGAPLPTFAHLPMILDEQGAKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHCCEEECCCCCCHHH
RTGAADVMQYRDSGYLPHALINYLVRLGWSHGDQELFNRQALIDLFQINDVNSKAARLDM
HCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHH
AKLGWVNQHYLKTDDPATLAPPLVWHLEQRGIDVSAGPAPTDVILALRERVQTLKEMAEK
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AEIWYCPLQRYDEIAVAKHLKPGAETALLHARTLLAALPAWTVDNVDTALRTTATTLEIG
CCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECC
MGKVAQPLRVAITGTQVSPDIAYTVYLTGRNEALKRIDAALIKISTA
CCCCCCCEEEEEECCEECCCEEEEEEEECCHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA