Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 182682424

GI number: 182682424

Start: 2126778

End: 2128433

Strand: Direct

Name: pdhB [H]

Synonym: XfasM23_1910

Alternate gene names: 182682424

Gene position: 2126778-2128433 (Clockwise)

Preceding gene: 182682422

Following gene: 182682425

Centisome position: 83.87

GC content: 52.6

Gene sequence:

>1656_bases
ATGACCGAAATCAAGGAAGCTTTTGTCCCCGATATCGGTGACTACAGCAACGTCCCAGTGATTGAAGTGCTAGTCGCTGT
TGGCGATACCGTCTCCAAGGGCCAAAGTTTGATCACCTTGGAATCTGACAAAGCAACCATGGAAGTACCTTCGTCAACAG
CCGGTATCATCAAAGAAATAAAAGTGAAGGTTGGCGATACCTTATCCCAAGGCCATGTCGTCGCATTAATCGAAGTCTCC
GAAGAGACTGCCGAAGTCACCACACCCATGACCGTTAACACGCCAACCACGCGTGCTCACAACACTGCACATCCAGAAGA
CGAAGGCAGCTTAATCGAAGTGCGTGTCCCTGATATTGGCGATTACACCAACGTCCCAGTGATTGAAGTCCTGGTCGCTG
TTGGCGATACCGTCTCCAAGGACCAAAGTCTAATCACCTTAGAATCTGACAAAGCAACCTTGGAAGTGCCCTCCTCCGCC
ACCGGTGTCATCAAACAACTCAAGGTCAACATTGGAGACACCTTGTCGCAAGGCGATATCGTCGTTGTGCTTCAAAGCGC
AGAGAGCACCCCACTCACTCCAACCACAAACAAACCAGCATTGCCACCCAATTCCAGCCCTGCAGGGGATGCCATCTCCG
CCCCAGCATCCCCTGCAGGGACACAGAGCACCCCACCGATCACCTTCGACGCCAACACCGTGCTACCGTCCAAGGTCCCC
TATGCCAGCCCCGCCGTACGCCTGCTTGCACGCCAGTTAGACGTGGACCTGAGCCGCATCAAAGGCAGCGAGAAAAATGG
ACGCATTACCCGCGAAGACGTACAGAAATTCGTCAACACCACCTTAGGTACTGGTATGGCTGAAGGCAGAACACCCTCCG
CTTCAATCACCACAAATGGTGGCCTCAACTTACTCCCTTGGCCGAATGTGGATTTCAGCAAATTCGGCGAAATTGAAACC
CAGCCTTTATCTCGGATCAAAAAGATTTCCGGTGCCAATCTTGCCCGCAATTGGGCGATGATCCCGCACGTCACCCAATT
TGATCAGGCCGACATCACCCAACTGGAAGCCCTGCGCGTACAAATCAATAAAGAGAATGCCGCATCCAACAAGAACGGAA
CCGGCATTAAACTGACTGTTCTGGCTTTCCTTATCAAAGCAAGCGCCTCAGCACTGAAAAAATTTCCAACATTCAACGCC
TCCCTTGATGCCACTGGCGAAACGCTCACACTCAAAAAATACTTCCATATCGGTTTCGCCGCCGACACACCAAACGGACT
CGTCGTCCCGGTGATCCGCAACGTAGACCAAAAAGGCATCGTAGAGATCGCACAAGAAACCAGCGATCTCGCCAAAAAAG
CACGCGATGGCAAACTCACCCCAGCAGACATGAGCGGTGGTTGCTTCTCCATCAGCTCACTCGGTGGCATCGGAGGTACC
GCATTCACACCCATTATCAACGCACCTGAAGTAGCGATCCTGGGTGTCTCCAAATCCCTCATCCAACCCATCTGGGATGG
CACACAATTCGCACCTAAAATGCTGTTGCCGCTGTCACTAAGCTACGATCACCGCGTCATTGATGGTGCCTTGGCCGCAC
ACTTCACCACTTATCTCAGCCAGATACTCGCCGACATGCGGCGCGTACTGCTGTGA

Upstream 100 bases:

>100_bases
GACACCGAAGCTCATTTTTGACATCATTACCCACTGCTTGGCGCTGCACCCTGAGCCAGCCACAATAGACACCCCTATTG
TTTATAGCCGGAGTACTGTA

Downstream 100 bases:

>100_bases
CAAAGACGCTGACCAACACAAACCACCGAGCGACAGGAGACACCTATGACGGTGATTGAAATCAAAGTTCCCGATATCGG
CGATTACAGCAAGGTCCCAG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTEIKEAFVPDIGDYSNVPVIEVLVAVGDTVSKGQSLITLESDKATMEVPSSTAGIIKEIKVKVGDTLSQGHVVALIEVS
EETAEVTTPMTVNTPTTRAHNTAHPEDEGSLIEVRVPDIGDYTNVPVIEVLVAVGDTVSKDQSLITLESDKATLEVPSSA
TGVIKQLKVNIGDTLSQGDIVVVLQSAESTPLTPTTNKPALPPNSSPAGDAISAPASPAGTQSTPPITFDANTVLPSKVP
YASPAVRLLARQLDVDLSRIKGSEKNGRITREDVQKFVNTTLGTGMAEGRTPSASITTNGGLNLLPWPNVDFSKFGEIET
QPLSRIKKISGANLARNWAMIPHVTQFDQADITQLEALRVQINKENAASNKNGTGIKLTVLAFLIKASASALKKFPTFNA
SLDATGETLTLKKYFHIGFAADTPNGLVVPVIRNVDQKGIVEIAQETSDLAKKARDGKLTPADMSGGCFSISSLGGIGGT
AFTPIINAPEVAILGVSKSLIQPIWDGTQFAPKMLLPLSLSYDHRVIDGALAAHFTTYLSQILADMRRVLL

Sequences:

>Translated_551_residues
MTEIKEAFVPDIGDYSNVPVIEVLVAVGDTVSKGQSLITLESDKATMEVPSSTAGIIKEIKVKVGDTLSQGHVVALIEVS
EETAEVTTPMTVNTPTTRAHNTAHPEDEGSLIEVRVPDIGDYTNVPVIEVLVAVGDTVSKDQSLITLESDKATLEVPSSA
TGVIKQLKVNIGDTLSQGDIVVVLQSAESTPLTPTTNKPALPPNSSPAGDAISAPASPAGTQSTPPITFDANTVLPSKVP
YASPAVRLLARQLDVDLSRIKGSEKNGRITREDVQKFVNTTLGTGMAEGRTPSASITTNGGLNLLPWPNVDFSKFGEIET
QPLSRIKKISGANLARNWAMIPHVTQFDQADITQLEALRVQINKENAASNKNGTGIKLTVLAFLIKASASALKKFPTFNA
SLDATGETLTLKKYFHIGFAADTPNGLVVPVIRNVDQKGIVEIAQETSDLAKKARDGKLTPADMSGGCFSISSLGGIGGT
AFTPIINAPEVAILGVSKSLIQPIWDGTQFAPKMLLPLSLSYDHRVIDGALAAHFTTYLSQILADMRRVLL
>Mature_550_residues
TEIKEAFVPDIGDYSNVPVIEVLVAVGDTVSKGQSLITLESDKATMEVPSSTAGIIKEIKVKVGDTLSQGHVVALIEVSE
ETAEVTTPMTVNTPTTRAHNTAHPEDEGSLIEVRVPDIGDYTNVPVIEVLVAVGDTVSKDQSLITLESDKATLEVPSSAT
GVIKQLKVNIGDTLSQGDIVVVLQSAESTPLTPTTNKPALPPNSSPAGDAISAPASPAGTQSTPPITFDANTVLPSKVPY
ASPAVRLLARQLDVDLSRIKGSEKNGRITREDVQKFVNTTLGTGMAEGRTPSASITTNGGLNLLPWPNVDFSKFGEIETQ
PLSRIKKISGANLARNWAMIPHVTQFDQADITQLEALRVQINKENAASNKNGTGIKLTVLAFLIKASASALKKFPTFNAS
LDATGETLTLKKYFHIGFAADTPNGLVVPVIRNVDQKGIVEIAQETSDLAKKARDGKLTPADMSGGCFSISSLGGIGGTA
FTPIINAPEVAILGVSKSLIQPIWDGTQFAPKMLLPLSLSYDHRVIDGALAAHFTTYLSQILADMRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=456, Percent_Identity=31.359649122807, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI31711992, Length=503, Percent_Identity=26.8389662027833, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=34.4827586206897, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI203098816, Length=434, Percent_Identity=26.9585253456221, Blast_Score=123, Evalue=6e-28,
Organism=Homo sapiens, GI203098753, Length=434, Percent_Identity=26.9585253456221, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI260898739, Length=149, Percent_Identity=38.255033557047, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1786305, Length=544, Percent_Identity=47.9779411764706, Blast_Score=480, Evalue=1e-136,
Organism=Escherichia coli, GI1786946, Length=418, Percent_Identity=31.3397129186603, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=449, Percent_Identity=30.0668151447661, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=27.7136258660508, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=436, Percent_Identity=30.2752293577982, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=30.5466237942122, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=27.2076372315036, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=437, Percent_Identity=25.1716247139588, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI18859875, Length=452, Percent_Identity=30.9734513274336, Blast_Score=181, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24645909, Length=213, Percent_Identity=35.6807511737089, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=31.4655172413793, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=31.4655172413793, Blast_Score=119, Evalue=6e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58297; Mature: 58166

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIKEAFVPDIGDYSNVPVIEVLVAVGDTVSKGQSLITLESDKATMEVPSSTAGIIKEI
CCCCHHHHCCCCCCCCCCHHHHHHHHHCCHHHCCCEEEEEECCCCEEECCCCHHHHHHHH
KVKVGDTLSQGHVVALIEVSEETAEVTTPMTVNTPTTRAHNTAHPEDEGSLIEVRVPDIG
HHHHCCCCCCCCEEEEEEECCCCHHCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCC
DYTNVPVIEVLVAVGDTVSKDQSLITLESDKATLEVPSSATGVIKQLKVNIGDTLSQGDI
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCCCCCCCE
VVVLQSAESTPLTPTTNKPALPPNSSPAGDAISAPASPAGTQSTPPITFDANTVLPSKVP
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
YASPAVRLLARQLDVDLSRIKGSEKNGRITREDVQKFVNTTLGTGMAEGRTPSASITTNG
CCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
GLNLLPWPNVDFSKFGEIETQPLSRIKKISGANLARNWAMIPHVTQFDQADITQLEALRV
CEEECCCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHCCEECCCCCCCCCCHHHHEEEEEE
QINKENAASNKNGTGIKLTVLAFLIKASASALKKFPTFNASLDATGETLTLKKYFHIGFA
EEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEE
ADTPNGLVVPVIRNVDQKGIVEIAQETSDLAKKARDGKLTPADMSGGCFSISSLGGIGGT
CCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCC
AFTPIINAPEVAILGVSKSLIQPIWDGTQFAPKMLLPLSLSYDHRVIDGALAAHFTTYLS
HHCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
QILADMRRVLL
HHHHHHHHHHC
>Mature Secondary Structure 
TEIKEAFVPDIGDYSNVPVIEVLVAVGDTVSKGQSLITLESDKATMEVPSSTAGIIKEI
CCCHHHHCCCCCCCCCCHHHHHHHHHCCHHHCCCEEEEEECCCCEEECCCCHHHHHHHH
KVKVGDTLSQGHVVALIEVSEETAEVTTPMTVNTPTTRAHNTAHPEDEGSLIEVRVPDIG
HHHHCCCCCCCCEEEEEEECCCCHHCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCC
DYTNVPVIEVLVAVGDTVSKDQSLITLESDKATLEVPSSATGVIKQLKVNIGDTLSQGDI
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEECCCCHHHHHHHHHHCCCCCCCCCCE
VVVLQSAESTPLTPTTNKPALPPNSSPAGDAISAPASPAGTQSTPPITFDANTVLPSKVP
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
YASPAVRLLARQLDVDLSRIKGSEKNGRITREDVQKFVNTTLGTGMAEGRTPSASITTNG
CCCHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
GLNLLPWPNVDFSKFGEIETQPLSRIKKISGANLARNWAMIPHVTQFDQADITQLEALRV
CEEECCCCCCCHHHCCCCCHHHHHHHHHHCCCCHHHCCEECCCCCCCCCCHHHHEEEEEE
QINKENAASNKNGTGIKLTVLAFLIKASASALKKFPTFNASLDATGETLTLKKYFHIGFA
EEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEE
ADTPNGLVVPVIRNVDQKGIVEIAQETSDLAKKARDGKLTPADMSGGCFSISSLGGIGGT
CCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCC
AFTPIINAPEVAILGVSKSLIQPIWDGTQFAPKMLLPLSLSYDHRVIDGALAAHFTTYLS
HHCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
QILADMRRVLL
HHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]