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The map label for this gene is topA [H]

Identifier: 182682383

GI number: 182682383

Start: 2074117

End: 2076564

Strand: Direct

Name: topA [H]

Synonym: XfasM23_1869

Alternate gene names: 182682383

Gene position: 2074117-2076564 (Clockwise)

Preceding gene: 182682382

Following gene: 182682384

Centisome position: 81.8

GC content: 51.63

Gene sequence:

>2448_bases
ATGCCCAAACACCTTCTTATCGTCGAATCGCCCGCCAAGGCCAAGACGATCAATAAATATCTCGGCAAAGATTTCACAGT
CTTAGCCTCTTACGGGCATGTACGCGATTTGGTACCGAAGGAAGGCGCAGTCGATCCAGAAAACGGCTTTGCGATGCGCT
ACGACCTCATCGACAAGAACGAAAAGCACGTCGAAGCAATCACAAAAGCGGCCAAAACAGCCGACAGCATCTATCTAGCA
ACCGACCCTGATCGCGAAGGCGAAGCGATCAGCTGGCACATCTCCGAGATCCTTAAAGAACGTGGATTATTGAAGGACAA
ACCGATGCAACGCATCGTGTTCACCGAGATCACACCACGCGCAATTAAAGAAGCAATTCAAAAGCCGCGGATGATTGCCA
GCGACCTGGTAGACGCCCAACAAGCACGCCGTGCGCTGGATTACTTGGTTGGCTTCAACCTCTCACCAGTGCTGTGGCGC
AAAGTACAACGCGGTTTATCGGCAGGGCGTGTGCAATCGCCAGCACTGCGCATGATTGTCGAGCGTGAAGAGGAAATTGA
GGCATTCATCACGCGTGAATACTGGAGCATCCATGCCGAGTGTACCCATCCCGCCCAACACTTCAGCGCCAAACTCATCA
AACTGGATGGGAAAAAATTCGAGCAATTCACTATTACCGATAGCGACACTGCCGCAGCCGCACAACGCAGGATCCAGCAG
GCCGCACAGGGACGCCTGCACATCACTGACGTTACCAACAAAGAACGGAAACGACGCCCTGCACCGCCCTTCATCACATC
CACATTACAGCAAGAAGCCTCACGCAAACTGGGTTTCACTACACGTAAAACGATGCAGATTGCGCAAAAACTTTACGAGG
GGATCGCACTGGGTGAGGAAGGCAGCGTCGGCCTGATTACGTATATGCGTACCGACTCAGTCAACCTGTCGCTGGATGCT
CTATCGGAGATCCGCGACATCATCGCACGCGACTACGGTACCAATGCACTTCCAGACAAACCTAACGTCTACACCACCAA
ATCCAAAAACGCACAAGAAGCACACGAAGCAGTACGCCCGACTTCAGCACTACGCACCCCGACACAGGTCGCACCGTATC
TTTCCAACGAAGAACACCGGCTATACGAACTGGTGTGGAAACGTACAGTAGCCTCCCAAATGATCCCAGCCATACTCAAT
ACCACCAGTGTCGATCTAGCCGCCGGCAACGAACACGTATTCCGTGCCACCGGAACCACCGTCGTAGTACAAGGTTTCCT
AGCTGTGTACGAAGAAGGCAAGGACAACAAGAACGCCGAAGACGATGACGAAGGCCGCAAACTCCCCGTAATGAAAACAG
GGGAAAATGTTCCGCTAGAACGTATCCTGACCGAACAACACTTTACACAGCCACCACCACGCTACACCGAAGCAGCGTTG
GTCAAAGCTCTGGAAGAATACGGCATCGGTCGCCCATCGACTTACGCCTCCATCATCCAAACCCTACTATTTCGCAAATA
CGTAGATATGGAAGGTCGCAGCTTCCGCCCAACGGACATTGGCCGCGCCGTATCCAAATTTCTCTCCAGCCACTTCACCC
AGTACGTGGACTACGACTTCACCGCACACCTGGAAGATGAATTGGATGCGATCTCACGTGGGGAGGAAGAATGGATTCCA
CTGATGAAAAAGTTTTGGGTCCCCTTCAAAGAATTGGTTGAAGATAAGAAAGATTCCCTAGATAAAACCGACGCTGGCAG
CGTGCGCCTGCTCGGCATTGACCCCACCAGCGGCAAAGAAGTCAGCGCACGCATTGGCCGCTTCGGACCGATGGTGCAAA
TCGGCACCGTTGATGATGAAGAAAAACCAAGATTTGCCTCATTACGCCCCAACCAAAGCATCTACTCAATCAGCCTCGAA
GAAGCCATCGAACTGTTCAAAATGCCACGCGTGCTTGGAGAAGACCAAAGCCAACAGGTCAGCGTCGGCATCGGCCGCTT
TGGACCGTTTGCCAAACGTGGCAGCACTTATGTTTCACTGAAAAGCGAAGACGATCCCTACACGATTGACTTGGCGCGCG
CGACCCTTCTCATCAACGAGAAAGAAGAAATCGCACGTAATCGAATCATCAAAGACTTCGAAAACAGCCAGATCCAAGTA
CTGAACGGACGCTTCGGTCCCTACATCAGCGATGGCAAACTTAACGGGAAGATCCCCAAGGATCGCGAACCAGCCTCGCT
GACTCTCGAAGAAGCACAGCAATTACTGATAAACACTGGGAAACCGGCACGCAAAAACTTCAGTACGAAAAAAACTGCAA
CCAAGAACGAGACGCGCAAACAAACCACAAAAAAACGCACTACGGACGCAAAGGCAACCAAAAAAGTGTCAGATAAGCCC
GTAAAGAAACAAATCAAAAAACGCATCGCCCCCAACATTACCCAATAA

Upstream 100 bases:

>100_bases
TCACTTCCACGTTTGCAACACACGGGTTCTGCTTCCCTCTAAAGGCTAATTAAGCCACGCTACCCGCCCCTAGCTCAGTC
ACTCCAAGACGTTCAGCACC

Downstream 100 bases:

>100_bases
ACGCCATTCCAACCCAGCATTCACCCCCCTGACAGAACCACCTATGCTATGCCTCTCACTATTCACACAGACAGCAATCA
AAGATCATGGCAACTCAGTG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 815; Mature: 814

Protein sequence:

>815_residues
MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPENGFAMRYDLIDKNEKHVEAITKAAKTADSIYLA
TDPDREGEAISWHISEILKERGLLKDKPMQRIVFTEITPRAIKEAIQKPRMIASDLVDAQQARRALDYLVGFNLSPVLWR
KVQRGLSAGRVQSPALRMIVEREEEIEAFITREYWSIHAECTHPAQHFSAKLIKLDGKKFEQFTITDSDTAAAAQRRIQQ
AAQGRLHITDVTNKERKRRPAPPFITSTLQQEASRKLGFTTRKTMQIAQKLYEGIALGEEGSVGLITYMRTDSVNLSLDA
LSEIRDIIARDYGTNALPDKPNVYTTKSKNAQEAHEAVRPTSALRTPTQVAPYLSNEEHRLYELVWKRTVASQMIPAILN
TTSVDLAAGNEHVFRATGTTVVVQGFLAVYEEGKDNKNAEDDDEGRKLPVMKTGENVPLERILTEQHFTQPPPRYTEAAL
VKALEEYGIGRPSTYASIIQTLLFRKYVDMEGRSFRPTDIGRAVSKFLSSHFTQYVDYDFTAHLEDELDAISRGEEEWIP
LMKKFWVPFKELVEDKKDSLDKTDAGSVRLLGIDPTSGKEVSARIGRFGPMVQIGTVDDEEKPRFASLRPNQSIYSISLE
EAIELFKMPRVLGEDQSQQVSVGIGRFGPFAKRGSTYVSLKSEDDPYTIDLARATLLINEKEEIARNRIIKDFENSQIQV
LNGRFGPYISDGKLNGKIPKDREPASLTLEEAQQLLINTGKPARKNFSTKKTATKNETRKQTTKKRTTDAKATKKVSDKP
VKKQIKKRIAPNITQ

Sequences:

>Translated_815_residues
MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPENGFAMRYDLIDKNEKHVEAITKAAKTADSIYLA
TDPDREGEAISWHISEILKERGLLKDKPMQRIVFTEITPRAIKEAIQKPRMIASDLVDAQQARRALDYLVGFNLSPVLWR
KVQRGLSAGRVQSPALRMIVEREEEIEAFITREYWSIHAECTHPAQHFSAKLIKLDGKKFEQFTITDSDTAAAAQRRIQQ
AAQGRLHITDVTNKERKRRPAPPFITSTLQQEASRKLGFTTRKTMQIAQKLYEGIALGEEGSVGLITYMRTDSVNLSLDA
LSEIRDIIARDYGTNALPDKPNVYTTKSKNAQEAHEAVRPTSALRTPTQVAPYLSNEEHRLYELVWKRTVASQMIPAILN
TTSVDLAAGNEHVFRATGTTVVVQGFLAVYEEGKDNKNAEDDDEGRKLPVMKTGENVPLERILTEQHFTQPPPRYTEAAL
VKALEEYGIGRPSTYASIIQTLLFRKYVDMEGRSFRPTDIGRAVSKFLSSHFTQYVDYDFTAHLEDELDAISRGEEEWIP
LMKKFWVPFKELVEDKKDSLDKTDAGSVRLLGIDPTSGKEVSARIGRFGPMVQIGTVDDEEKPRFASLRPNQSIYSISLE
EAIELFKMPRVLGEDQSQQVSVGIGRFGPFAKRGSTYVSLKSEDDPYTIDLARATLLINEKEEIARNRIIKDFENSQIQV
LNGRFGPYISDGKLNGKIPKDREPASLTLEEAQQLLINTGKPARKNFSTKKTATKNETRKQTTKKRTTDAKATKKVSDKP
VKKQIKKRIAPNITQ
>Mature_814_residues
PKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPENGFAMRYDLIDKNEKHVEAITKAAKTADSIYLAT
DPDREGEAISWHISEILKERGLLKDKPMQRIVFTEITPRAIKEAIQKPRMIASDLVDAQQARRALDYLVGFNLSPVLWRK
VQRGLSAGRVQSPALRMIVEREEEIEAFITREYWSIHAECTHPAQHFSAKLIKLDGKKFEQFTITDSDTAAAAQRRIQQA
AQGRLHITDVTNKERKRRPAPPFITSTLQQEASRKLGFTTRKTMQIAQKLYEGIALGEEGSVGLITYMRTDSVNLSLDAL
SEIRDIIARDYGTNALPDKPNVYTTKSKNAQEAHEAVRPTSALRTPTQVAPYLSNEEHRLYELVWKRTVASQMIPAILNT
TSVDLAAGNEHVFRATGTTVVVQGFLAVYEEGKDNKNAEDDDEGRKLPVMKTGENVPLERILTEQHFTQPPPRYTEAALV
KALEEYGIGRPSTYASIIQTLLFRKYVDMEGRSFRPTDIGRAVSKFLSSHFTQYVDYDFTAHLEDELDAISRGEEEWIPL
MKKFWVPFKELVEDKKDSLDKTDAGSVRLLGIDPTSGKEVSARIGRFGPMVQIGTVDDEEKPRFASLRPNQSIYSISLEE
AIELFKMPRVLGEDQSQQVSVGIGRFGPFAKRGSTYVSLKSEDDPYTIDLARATLLINEKEEIARNRIIKDFENSQIQVL
NGRFGPYISDGKLNGKIPKDREPASLTLEEAQQLLINTGKPARKNFSTKKTATKNETRKQTTKKRTTDAKATKKVSDKPV
KKQIKKRIAPNITQ

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=546, Percent_Identity=24.5421245421245, Blast_Score=118, Evalue=3e-26,
Organism=Homo sapiens, GI4507635, Length=523, Percent_Identity=22.7533460803059, Blast_Score=92, Evalue=3e-18,
Organism=Escherichia coli, GI1787529, Length=646, Percent_Identity=39.938080495356, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1788061, Length=557, Percent_Identity=26.5709156193896, Blast_Score=140, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32563869, Length=597, Percent_Identity=26.8006700167504, Blast_Score=137, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=520, Percent_Identity=25.3846153846154, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=519, Percent_Identity=23.5067437379576, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585251, Length=535, Percent_Identity=24.4859813084112, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640096, Length=519, Percent_Identity=23.3140655105973, Blast_Score=96, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 91803; Mature: 91671

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPENGFAMRYDLIDKN
CCCCEEEEECCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCEEEEECCCCC
EKHVEAITKAAKTADSIYLATDPDREGEAISWHISEILKERGLLKDKPMQRIVFTEITPR
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCHH
AIKEAIQKPRMIASDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
EREEEIEAFITREYWSIHAECTHPAQHFSAKLIKLDGKKFEQFTITDSDTAAAAQRRIQQ
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHHHHH
AAQGRLHITDVTNKERKRRPAPPFITSTLQQEASRKLGFTTRKTMQIAQKLYEGIALGEE
HHCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
GSVGLITYMRTDSVNLSLDALSEIRDIIARDYGTNALPDKPNVYTTKSKNAQEAHEAVRP
CCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHCC
TSALRTPTQVAPYLSNEEHRLYELVWKRTVASQMIPAILNTTSVDLAAGNEHVFRATGTT
HHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCH
VVVQGFLAVYEEGKDNKNAEDDDEGRKLPVMKTGENVPLERILTEQHFTQPPPRYTEAAL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHH
VKALEEYGIGRPSTYASIIQTLLFRKYVDMEGRSFRPTDIGRAVSKFLSSHFTQYVDYDF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TAHLEDELDAISRGEEEWIPLMKKFWVPFKELVEDKKDSLDKTDAGSVRLLGIDPTSGKE
EECCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCH
VSARIGRFGPMVQIGTVDDEEKPRFASLRPNQSIYSISLEEAIELFKMPRVLGEDQSQQV
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEE
SVGIGRFGPFAKRGSTYVSLKSEDDPYTIDLARATLLINEKEEIARNRIIKDFENSQIQV
EEECCCCCCHHHCCCEEEEEECCCCCEEEEEEHEEEEECCHHHHHHHHHHHHCCCCEEEE
LNGRFGPYISDGKLNGKIPKDREPASLTLEEAQQLLINTGKPARKNFSTKKTATKNETRK
EECCCCCEECCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHH
QTTKKRTTDAKATKKVSDKPVKKQIKKRIAPNITQ
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PKHLLIVESPAKAKTINKYLGKDFTVLASYGHVRDLVPKEGAVDPENGFAMRYDLIDKN
CCCEEEEECCCHHHHHHHHHCCCEEEEECCCHHHHCCCCCCCCCCCCCCEEEEECCCCC
EKHVEAITKAAKTADSIYLATDPDREGEAISWHISEILKERGLLKDKPMQRIVFTEITPR
HHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHCCCHH
AIKEAIQKPRMIASDLVDAQQARRALDYLVGFNLSPVLWRKVQRGLSAGRVQSPALRMIV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHH
EREEEIEAFITREYWSIHAECTHPAQHFSAKLIKLDGKKFEQFTITDSDTAAAAQRRIQQ
HHHHHHHHHHHHHHHEEECCCCCCHHHHHHEEEEECCCCEEEEEECCCHHHHHHHHHHHH
AAQGRLHITDVTNKERKRRPAPPFITSTLQQEASRKLGFTTRKTMQIAQKLYEGIALGEE
HHCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
GSVGLITYMRTDSVNLSLDALSEIRDIIARDYGTNALPDKPNVYTTKSKNAQEAHEAVRP
CCEEEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHCC
TSALRTPTQVAPYLSNEEHRLYELVWKRTVASQMIPAILNTTSVDLAAGNEHVFRATGTT
HHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCH
VVVQGFLAVYEEGKDNKNAEDDDEGRKLPVMKTGENVPLERILTEQHFTQPPPRYTEAAL
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCCCHHHHHH
VKALEEYGIGRPSTYASIIQTLLFRKYVDMEGRSFRPTDIGRAVSKFLSSHFTQYVDYDF
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
TAHLEDELDAISRGEEEWIPLMKKFWVPFKELVEDKKDSLDKTDAGSVRLLGIDPTSGKE
EECCHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCH
VSARIGRFGPMVQIGTVDDEEKPRFASLRPNQSIYSISLEEAIELFKMPRVLGEDQSQQV
HHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCEE
SVGIGRFGPFAKRGSTYVSLKSEDDPYTIDLARATLLINEKEEIARNRIIKDFENSQIQV
EEECCCCCCHHHCCCEEEEEECCCCCEEEEEEHEEEEECCHHHHHHHHHHHHCCCCEEEE
LNGRFGPYISDGKLNGKIPKDREPASLTLEEAQQLLINTGKPARKNFSTKKTATKNETRK
EECCCCCEECCCCCCCCCCCCCCCCCEEHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHH
QTTKKRTTDAKATKKVSDKPVKKQIKKRIAPNITQ
HHHHHHCCHHHHHHHCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]