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The map label for this gene is pilQ [H]

Identifier: 182682302

GI number: 182682302

Start: 1982380

End: 1984290

Strand: Reverse

Name: pilQ [H]

Synonym: XfasM23_1785

Alternate gene names: 182682302

Gene position: 1984290-1982380 (Counterclockwise)

Preceding gene: 182682303

Following gene: 182682301

Centisome position: 78.25

GC content: 48.46

Gene sequence:

>1911_bases
ATGATTGATTTAAGGGATAGAATGATGACTTTATTTGAAGTCTTAGGCTTGCGTTTTGTTAGGCGTATAGCCTCGCTTCG
CGTATGTGAAATTTGGGTATGCATAGCTTTCGCTTGGTGTTGCATCCCAGGAACCGTATCGGCTGAAAACAAGCAAGCGA
TTCCAGTGGCTAAAGCTACCAATGCGCCACTAAGCGTTTCCAAAATCGATTTCAAACGTGGTGATGATGGCTCTGGCCGT
CTGATTTTGAAGTTTGATGGGCAGGGAGCCACTCCTGACCTGCGTACGCAGGGTGGCACCGTGCTGGTTGACCTGGGGAC
AGCTGTGTTGCCGACTGTCTTGCAACGCCAGATCAACGTTGTTGACTTTGCTACCCCAGTGCAGCGTATCGACGCTAAGC
CTATGGGTAAGGGTGCACAACTTGTGTTGAGCACGAAGGGAGCGTTTGAGTCGCTTGCTTACCAGACAGGGGATGAATAC
GTGGTTGAAATTGTGCCACGCAAGGGAGAGGCTGCCATGGGTGGGGCGATCACCCCAGAATCGGTCAGTAAGTCCGCAGC
GAAGATTTCCGCACAAGGGTACAGTGGTCGTCCAGTAACGTTTAACTTCCAAGACGTTCCAGTACGAACGGTTTTGCAGT
TGATCGCTGATGAGTCCAATCTCAATATTGTTGCTTCCGACACTGTGCAAGGTAATGTCACTCTACGTTTGATTAATGTG
CCATGGGATCAAGCGCTCGACATCGTTTTGCGTGCTAAAGGATTGGATAAGCGCCGTGATGAGAAAGTAATTTGGGTTGC
GCCGCAACAGGAGCTTGCTAAATACGAGCAAGAAAAAGAGGACGCGCGGATCGCGATCGAAAATCGTGAGGGGTTAATTA
CCGATTATGTCCAAATAAATTATCATTCGGCCACGGTCATTTTTAAGGCACTGACAGAAGCCAAAGGTATGGGTGGGGGA
GGTAACGGTACACCTAATAATAATAGTGATGATGCTTTCTTATCGCCACGTGGTCGGCTTGTGGCTGATGAGCGCACTAA
TACGTTGATGATTAGCGATATTCCGAAGAAAGTTGCGCAAATGCGTACTCTTATCCAGCATATTGATCGTCCTGTGGACC
AAGTGTTGATTGAGGGCCGCATCGTGATCGCCACCGATAGCTTCGCGCGTGATTTGGGGGCCAAGTTCGGTGTTGGTGCT
GCTAGTCGTTTCAGCGATAACACTGCAACGATCGGTAGTAATGTGACTGCTGGCAGCTCCACATCGGCTACGCGGGGGCT
TAATGTTGATTTGGGCGGTCCGACCAACACCACTGCTTCAGTTCTTCCGAGTTTAGCCTACACTTTGCTTGGACCTAAAT
TTAATCTAGATCTTGAGCTTTCGGCATTGCAGCAGGAATCCCGTGGCGAGGTGATTTCAAACCCTCGTATTGTCACTGCA
AACCAGCGTGAGGGATATATCAAACAAGGTAAGGAAATTGGGTATGTCACGATCACTGGAGGAGTCGCTGGTGGTCAAGC
CACTCCGAATGTCCAATTTAAAGAGGCTGTTTTGGAGTTGCGTGTGACCCCAACGATCACCAATGACAACCGTGTGTTTC
TCAACATGACTGTCAAGAAGGACGAGATTGATCAAATGATTACGATCGCCAATTTCGGTACCGTGCCGACCTTGAACAAG
CGTGAGATCAACACTGCCGTATTGGTTGATGATGGTCAAACTGTTGTTATCGGTGGTGTATATGAGTTTTCCGACCGTAA
TAGTGTGAGTAAGGTTCCGTTCTTAGGGGATGTGCCTTTCCTTGGTAATTTGTTTAAGAAACGTGGTAAAGATAAACAAA
AGGCCGAGCTTTTGATTTTCGTGACTCCTAAGGTGTTGCGTGTGGCTAATGCTACTGCTGCGGTGCATTGA

Upstream 100 bases:

>100_bases
TTACGAAGGTGAATGAAGATCGCGTCGAACTTGTGGAACTTGTTTCAAATGGCGAGGGAGGATGGTTGGAACGGCCAGCC
GCGCTTTCGTTCGACGATTA

Downstream 100 bases:

>100_bases
ATTTCGTCAATGCACTGCTTTATATCTCTGAAATAAACTCTTGTTCCAGTTCGTGTCTGCACTCGTGTTCTCATTGTGCA
ATTCGAGTCGAGTGTTGCCA

Product: type IV pilus secretin PilQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MIDLRDRMMTLFEVLGLRFVRRIASLRVCEIWVCIAFAWCCIPGTVSAENKQAIPVAKATNAPLSVSKIDFKRGDDGSGR
LILKFDGQGATPDLRTQGGTVLVDLGTAVLPTVLQRQINVVDFATPVQRIDAKPMGKGAQLVLSTKGAFESLAYQTGDEY
VVEIVPRKGEAAMGGAITPESVSKSAAKISAQGYSGRPVTFNFQDVPVRTVLQLIADESNLNIVASDTVQGNVTLRLINV
PWDQALDIVLRAKGLDKRRDEKVIWVAPQQELAKYEQEKEDARIAIENREGLITDYVQINYHSATVIFKALTEAKGMGGG
GNGTPNNNSDDAFLSPRGRLVADERTNTLMISDIPKKVAQMRTLIQHIDRPVDQVLIEGRIVIATDSFARDLGAKFGVGA
ASRFSDNTATIGSNVTAGSSTSATRGLNVDLGGPTNTTASVLPSLAYTLLGPKFNLDLELSALQQESRGEVISNPRIVTA
NQREGYIKQGKEIGYVTITGGVAGGQATPNVQFKEAVLELRVTPTITNDNRVFLNMTVKKDEIDQMITIANFGTVPTLNK
REINTAVLVDDGQTVVIGGVYEFSDRNSVSKVPFLGDVPFLGNLFKKRGKDKQKAELLIFVTPKVLRVANATAAVH

Sequences:

>Translated_636_residues
MIDLRDRMMTLFEVLGLRFVRRIASLRVCEIWVCIAFAWCCIPGTVSAENKQAIPVAKATNAPLSVSKIDFKRGDDGSGR
LILKFDGQGATPDLRTQGGTVLVDLGTAVLPTVLQRQINVVDFATPVQRIDAKPMGKGAQLVLSTKGAFESLAYQTGDEY
VVEIVPRKGEAAMGGAITPESVSKSAAKISAQGYSGRPVTFNFQDVPVRTVLQLIADESNLNIVASDTVQGNVTLRLINV
PWDQALDIVLRAKGLDKRRDEKVIWVAPQQELAKYEQEKEDARIAIENREGLITDYVQINYHSATVIFKALTEAKGMGGG
GNGTPNNNSDDAFLSPRGRLVADERTNTLMISDIPKKVAQMRTLIQHIDRPVDQVLIEGRIVIATDSFARDLGAKFGVGA
ASRFSDNTATIGSNVTAGSSTSATRGLNVDLGGPTNTTASVLPSLAYTLLGPKFNLDLELSALQQESRGEVISNPRIVTA
NQREGYIKQGKEIGYVTITGGVAGGQATPNVQFKEAVLELRVTPTITNDNRVFLNMTVKKDEIDQMITIANFGTVPTLNK
REINTAVLVDDGQTVVIGGVYEFSDRNSVSKVPFLGDVPFLGNLFKKRGKDKQKAELLIFVTPKVLRVANATAAVH
>Mature_636_residues
MIDLRDRMMTLFEVLGLRFVRRIASLRVCEIWVCIAFAWCCIPGTVSAENKQAIPVAKATNAPLSVSKIDFKRGDDGSGR
LILKFDGQGATPDLRTQGGTVLVDLGTAVLPTVLQRQINVVDFATPVQRIDAKPMGKGAQLVLSTKGAFESLAYQTGDEY
VVEIVPRKGEAAMGGAITPESVSKSAAKISAQGYSGRPVTFNFQDVPVRTVLQLIADESNLNIVASDTVQGNVTLRLINV
PWDQALDIVLRAKGLDKRRDEKVIWVAPQQELAKYEQEKEDARIAIENREGLITDYVQINYHSATVIFKALTEAKGMGGG
GNGTPNNNSDDAFLSPRGRLVADERTNTLMISDIPKKVAQMRTLIQHIDRPVDQVLIEGRIVIATDSFARDLGAKFGVGA
ASRFSDNTATIGSNVTAGSSTSATRGLNVDLGGPTNTTASVLPSLAYTLLGPKFNLDLELSALQQESRGEVISNPRIVTA
NQREGYIKQGKEIGYVTITGGVAGGQATPNVQFKEAVLELRVTPTITNDNRVFLNMTVKKDEIDQMITIANFGTVPTLNK
REINTAVLVDDGQTVVIGGVYEFSDRNSVSKVPFLGDVPFLGNLFKKRGKDKQKAELLIFVTPKVLRVANATAAVH

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=443, Percent_Identity=30.2483069977427, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI87082242, Length=293, Percent_Identity=29.0102389078498, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 68770; Mature: 68770

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLRDRMMTLFEVLGLRFVRRIASLRVCEIWVCIAFAWCCIPGTVSAENKQAIPVAKAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
NAPLSVSKIDFKRGDDGSGRLILKFDGQGATPDLRTQGGTVLVDLGTAVLPTVLQRQINV
CCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCE
VDFATPVQRIDAKPMGKGAQLVLSTKGAFESLAYQTGDEYVVEIVPRKGEAAMGGAITPE
EECCCHHHHCCCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHH
SVSKSAAKISAQGYSGRPVTFNFQDVPVRTVLQLIADESNLNIVASDTVQGNVTLRLINV
HHCCHHHEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCCCEEEEECCEECCEEEEEEEC
PWDQALDIVLRAKGLDKRRDEKVIWVAPQQELAKYEQEKEDARIAIENREGLITDYVQIN
CHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEE
YHSATVIFKALTEAKGMGGGGNGTPNNNSDDAFLSPRGRLVADERTNTLMISDIPKKVAQ
ECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCEEEEEHHHHHHHH
MRTLIQHIDRPVDQVLIEGRIVIATDSFARDLGAKFGVGAASRFSDNTATIGSNVTAGSS
HHHHHHHHCCCHHHHHHCCEEEEEECHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCC
TSATRGLNVDLGGPTNTTASVLPSLAYTLLGPKFNLDLELSALQQESRGEVISNPRIVTA
CCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEECCCEEEEE
NQREGYIKQGKEIGYVTITGGVAGGQATPNVQFKEAVLELRVTPTITNDNRVFLNMTVKK
CCCCCHHHCCCEEEEEEEECCCCCCCCCCCCHHHEEEEEEEEEEEEECCCEEEEEEEECH
DEIDQMITIANFGTVPTLNKREINTAVLVDDGQTVVIGGVYEFSDRNSVSKVPFLGDVPF
HHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCH
LGNLFKKRGKDKQKAELLIFVTPKVLRVANATAAVH
HHHHHHHCCCCCCCCCEEEEECCHHHHHHCCHHCCC
>Mature Secondary Structure
MIDLRDRMMTLFEVLGLRFVRRIASLRVCEIWVCIAFAWCCIPGTVSAENKQAIPVAKAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
NAPLSVSKIDFKRGDDGSGRLILKFDGQGATPDLRTQGGTVLVDLGTAVLPTVLQRQINV
CCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCE
VDFATPVQRIDAKPMGKGAQLVLSTKGAFESLAYQTGDEYVVEIVPRKGEAAMGGAITPE
EECCCHHHHCCCCCCCCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHH
SVSKSAAKISAQGYSGRPVTFNFQDVPVRTVLQLIADESNLNIVASDTVQGNVTLRLINV
HHCCHHHEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCCCEEEEECCEECCEEEEEEEC
PWDQALDIVLRAKGLDKRRDEKVIWVAPQQELAKYEQEKEDARIAIENREGLITDYVQIN
CHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEE
YHSATVIFKALTEAKGMGGGGNGTPNNNSDDAFLSPRGRLVADERTNTLMISDIPKKVAQ
ECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCEEEEEHHHHHHHH
MRTLIQHIDRPVDQVLIEGRIVIATDSFARDLGAKFGVGAASRFSDNTATIGSNVTAGSS
HHHHHHHHCCCHHHHHHCCEEEEEECHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCCC
TSATRGLNVDLGGPTNTTASVLPSLAYTLLGPKFNLDLELSALQQESRGEVISNPRIVTA
CCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEECCCEEEEE
NQREGYIKQGKEIGYVTITGGVAGGQATPNVQFKEAVLELRVTPTITNDNRVFLNMTVKK
CCCCCHHHCCCEEEEEEEECCCCCCCCCCCCHHHEEEEEEEEEEEEECCCEEEEEEEECH
DEIDQMITIANFGTVPTLNKREINTAVLVDDGQTVVIGGVYEFSDRNSVSKVPFLGDVPF
HHHHHEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCH
LGNLFKKRGKDKQKAELLIFVTPKVLRVANATAAVH
HHHHHHHCCCCCCCCCEEEEECCHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]