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The map label for this gene is thiE

Identifier: 182682298

GI number: 182682298

Start: 1975298

End: 1975912

Strand: Direct

Name: thiE

Synonym: XfasM23_1781

Alternate gene names: 182682298

Gene position: 1975298-1975912 (Clockwise)

Preceding gene: 182682295

Following gene: 182682299

Centisome position: 77.9

GC content: 52.36

Gene sequence:

>615_bases
ATGCCCCAGCCTCGCGGCATCTACCTAATTACTCCTGATGAAACTGACACTGCACGACTTATCGCACACACCGCACCATT
ACTGAATGGCATCGTTTGGCTGCAATACCGCAACAAACTAGCCAATACCGCATTACGCACAGAACAAGCACAGGCACTAC
TAGCCCTATGCCGTCCAACCGGCATCCCCTTACTCATCAACGACGACTTGGAATTAGCACAAACCATTGGCGCAGATGGT
GTCCACCTGGGCATGCATGACAGCAACGCCAGCATTGCGCGAGCACAATTAGGACCGCACGCCATCATAGGCGTCTCCTG
CTACAACCAAATTGAACGTGCGAAACAAGCAATCAAAGCCGGTGCCAGCTACGTAGGCTTCGGTGCGTTCTATCCGAGTC
ATACCAAAACCACCCCATACCGCGCCACACCAGAACTGCTACGCCAAACTACCCATTTGGGTGTACCACGCGTGGCCATC
GGCGGACTGACGCCAAAGAATATTGCTCCCATCATCGAAGCCGGTGCAGAGCTACTCGCAGTGATCAGCGGAATCTACTC
TGCAAAAAACCCAATCACTGCACTGAAAGCATATCAATCCCAATTCAACATCTGA

Upstream 100 bases:

>100_bases
CCACATCATTGTGGAAGTTTCAAGCATAGGATCATTCCAAGAACATATTGATAAGACCACTGACATTGTCACACTGCATC
CTCTACTTCGGAACCCCATC

Downstream 100 bases:

>100_bases
ACAAACCATCCAGCCACACACATTCACCGTCAACGTAACCAACCACTCCATCACATCAATTTTTGTTGATGTACCAACAC
ACCCTCGCCTCACTGACCAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPQPRGIYLITPDETDTARLIAHTAPLLNGIVWLQYRNKLANTALRTEQAQALLALCRPTGIPLLINDDLELAQTIGADG
VHLGMHDSNASIARAQLGPHAIIGVSCYNQIERAKQAIKAGASYVGFGAFYPSHTKTTPYRATPELLRQTTHLGVPRVAI
GGLTPKNIAPIIEAGAELLAVISGIYSAKNPITALKAYQSQFNI

Sequences:

>Translated_204_residues
MPQPRGIYLITPDETDTARLIAHTAPLLNGIVWLQYRNKLANTALRTEQAQALLALCRPTGIPLLINDDLELAQTIGADG
VHLGMHDSNASIARAQLGPHAIIGVSCYNQIERAKQAIKAGASYVGFGAFYPSHTKTTPYRATPELLRQTTHLGVPRVAI
GGLTPKNIAPIIEAGAELLAVISGIYSAKNPITALKAYQSQFNI
>Mature_203_residues
PQPRGIYLITPDETDTARLIAHTAPLLNGIVWLQYRNKLANTALRTEQAQALLALCRPTGIPLLINDDLELAQTIGADGV
HLGMHDSNASIARAQLGPHAIIGVSCYNQIERAKQAIKAGASYVGFGAFYPSHTKTTPYRATPELLRQTTHLGVPRVAIG
GLTPKNIAPIIEAGAELLAVISGIYSAKNPITALKAYQSQFNI

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=182, Percent_Identity=29.6703296703297, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=159, Percent_Identity=30.8176100628931, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 21870; Mature: 21738

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPRGIYLITPDETDTARLIAHTAPLLNGIVWLQYRNKLANTALRTEQAQALLALCRPT
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIPLLINDDLELAQTIGADGVHLGMHDSNASIARAQLGPHAIIGVSCYNQIERAKQAIKA
CCEEEECCCHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHC
GASYVGFGAFYPSHTKTTPYRATPELLRQTTHLGVPRVAIGGLTPKNIAPIIEAGAELLA
CCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHH
VISGIYSAKNPITALKAYQSQFNI
HHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
PQPRGIYLITPDETDTARLIAHTAPLLNGIVWLQYRNKLANTALRTEQAQALLALCRPT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIPLLINDDLELAQTIGADGVHLGMHDSNASIARAQLGPHAIIGVSCYNQIERAKQAIKA
CCEEEECCCHHHHHHCCCCCEEEEECCCCCCCHHHCCCCCEEEHHHHHHHHHHHHHHHHC
GASYVGFGAFYPSHTKTTPYRATPELLRQTTHLGVPRVAIGGLTPKNIAPIIEAGAELLA
CCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHH
VISGIYSAKNPITALKAYQSQFNI
HHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]