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The map label for this gene is clpB [H]

Identifier: 182682296

GI number: 182682296

Start: 1971938

End: 1974523

Strand: Reverse

Name: clpB [H]

Synonym: XfasM23_1779

Alternate gene names: 182682296

Gene position: 1974523-1971938 (Counterclockwise)

Preceding gene: 182682297

Following gene: 182682293

Centisome position: 77.87

GC content: 51.39

Gene sequence:

>2586_bases
ATGCGGATGGATAAGCTTACTTCGCGTTTCCAGAATGCGTTGGCTGATGCGCAATCGCTGGCCGTCGGTCGTGACCATAC
TATTATTGAGCCGGTGCATGTTTTTTCGGTATTGCTTGATCAGCAGGGGGGGAGTACACGTCCGTTGCTCGTGCAGGCCG
GTGTCAATGTGCCATTGCTGCGTGAGCGGTTGACCGAGATACTTGAGGCATTGCCGAAAGTGAGTGGTCAGACGGTTAAT
GTCTCACCCAGCAATGAATTGAGTCGTTTGTTTCATCGGACTGATAAGTTAGCGCAGCAGCATGGTGATCAGTTTATGGC
CAGTGAGTGGTTCGTGTTGGCTGTGGTCGATGATAGTGGGGGGCTGGGCCAGGCGTTGCGTGCTGCTGGTGCGGAAAAAA
AGAAGATTGAGGCTGCGATTGATAAATTGCGTGGTGGTGAAACTGTCCAGACTGAGAATGCGGAGGAGCAGCGTCAAGCG
TTGGAAAAGTACACGATTGATCTGACTGCCAGGGCTGAGAGTGGCAAGCTTGATCCGGTGATTGGTCGTGATGAGGAAAT
TCGCCGCACGATACAGGTATTGCAGCGGCGTACAAAAAATAATCCTGTGCTGATTGGTGAGCCGGGTGTGGGTAAAACTG
CCATTGTAGAAGGGCTGGCTCAGCGCATTGTCAATGGTGAGGTTCCGGAAGGGTTGCGCAGTAAGCGTCTGCTCTCGCTG
GATTTGGGGGCGTTGATTGCTGGGGCCAAGTTTCGTGGTGAGTTCGAGGAGCGCTTGAAGGGGGTGCTTAACGATCTCGC
TAAGAATGAGGGGCGGGTCATTTTGTTCATTGACGAGCTGCATACCATGGTTGGTGCCGGTAAAGCCGAGGGTGCGATGG
ATGCTGGCAATATGCTCAAGCCGGCGTTAGCACGTGGTGAGTTGCATTGTATCGGTGCGACTACGTTGGATGAGTATCGC
AAGTACATTGAGAAGGATGCGGCGTTGGAACGCCGTTTCCAGAAGGTGTTTGTTGGGGAGCCGACGGTGGAGGATACCAT
CGCAATTCTTCGTGGACTTAAGGAAAAGTATGCGTTGCACCACGGTGTGGAAATCACTGATCCGGCTATTGTTGCTGCGG
CTACGCTGTCTAATCGCTACATCACTGATCGTCAGTTACCAGATAAAGCGATTGACTTGATGGATGAGGCTGCCAGTCGT
ATTCGTATGGAAATTGACTCCAAGCCGGAAGAGCTTGATCGTTTGGAGCGTCGGTTGATTCAGTTGAAAATTCAGCGTGA
GATGCTGAAGAAAGAAAAGGATGAGGCGAGCAAGCAGCGTTTAGTCGATCTTGAGCGTGATATTGAGGTTCTGGACCGTG
AATTTTCCGATCTGGAGGAGGTGTGGAGATCAGAAAAAGCCGCGCTACAGGGGGCGACTAAGATCAAGGAGTCAATCGAG
CAGGCCAAGCTTGATTTGGAAGCCGCGCAGCGCCGTCAGGACTACGCCAAGATGAGCGAGATTCAGTATGGTGTACTCCC
CGCGTTGGAGAAGCAGCTAGTGGCAGCAAGTCAGGCGGAACAGCACGATTTTACTTTGGTGCAGGAGAAAGTGACTGCTG
AGGAGATTGCCGAGGTAGTCAGTCGTTGGACTGGTATTCCAGTGAGCAAGATGCTTGAGGGCGAGCGCGATAAGTTGTTG
CGTATGGAAGCTGATCTAGGTCGACGTGTGGTTGGTCAAGATGAGGCGATCAAGGTGGTATCGGATGCGGTGCGCCGTTC
GCGTACTGGTTTGTCTGATCCAAATCGACCGAGCGGCTCGTTCCTTTTTCTGGGTCCGACGGGTGTTGGTAAGACTGAGT
TATGTAAGGCGCTGGCTGAATTTTTGTTCGACAGTCAAGATGCAATGGTCCGCATTGATATGAGTGAGTTCATGGAAAAA
CATTCTGTGGCGCGCCTGATTGGTGCGCCTCCGGGCTATGTGGGTTATGAGGAAGGAGGTTATCTTACTGAATTGGTGCG
ACGTCGGCCTTACTCTCTGATTCTTTTGGATGAAGTGGAGAAGGCGCATAGTGATGTGTTCAATATTCTGCTGCAAGTAC
TTGATGATGGACGCTTGACTGATGGTCAGGGCCGTACTGTTGATTTTCGCAATACCGTTATTGTGATGACATCAAACTTA
GGTTCACATCAGATCCAAGAACTCAGTGGAGACGATTCTCCGGAGGTGTATACGCAAATGAAGGCGGCAGTGATGGGGGT
GGTGCAGGCGCATTTTCGCCCAGAATTCATTAACAGGTTGGATGATATTGTTGTTTTCCACCCGTTGGACAAGGCGCAAA
TCAAGCAGATTGCGCGGATTCAACTGCGGGGGCTAGAGAAGCGTCTCGCTGAATCTGAGTTGAAACTAGATTTGGATGAT
CGTGCGCTGGAATTGCTTGGTAATGTCGGATTTGATCCTGTATATGGTGCCCGCCCGCTGAAGAGAGCAATCCAATCTCA
GCTGGAGAATGCCTTAGCGCAGCAGATCTTAGCTGGAGCGTTCGTCAGTGGAGATACTGTGCAGGTGGGTGTTGATGGAG
GGAAGCTTGTATTCTCTAAGGTGTGA

Upstream 100 bases:

>100_bases
TGGTCATGTCATCGGTCGTGTCCTTGAAAGTGACTTGTTAAACCGCATCTGTTGGATGTCGCCGGCAGGTGTCCGGTCTC
TTTCCTAACTGGGGCTTTTT

Downstream 100 bases:

>100_bases
AGTTGTTTACTGTATATAGTCAGTAGTGCTGTGTATTGTATGGGTGTACGAAGGGTGTGGTTATAAATAACCTTTCTTGG
TTGCCAGTAATGTTGGTTTA

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MRMDKLTSRFQNALADAQSLAVGRDHTIIEPVHVFSVLLDQQGGSTRPLLVQAGVNVPLLRERLTEILEALPKVSGQTVN
VSPSNELSRLFHRTDKLAQQHGDQFMASEWFVLAVVDDSGGLGQALRAAGAEKKKIEAAIDKLRGGETVQTENAEEQRQA
LEKYTIDLTARAESGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLRSKRLLSL
DLGALIAGAKFRGEFEERLKGVLNDLAKNEGRVILFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYR
KYIEKDAALERRFQKVFVGEPTVEDTIAILRGLKEKYALHHGVEITDPAIVAAATLSNRYITDRQLPDKAIDLMDEAASR
IRMEIDSKPEELDRLERRLIQLKIQREMLKKEKDEASKQRLVDLERDIEVLDREFSDLEEVWRSEKAALQGATKIKESIE
QAKLDLEAAQRRQDYAKMSEIQYGVLPALEKQLVAASQAEQHDFTLVQEKVTAEEIAEVVSRWTGIPVSKMLEGERDKLL
RMEADLGRRVVGQDEAIKVVSDAVRRSRTGLSDPNRPSGSFLFLGPTGVGKTELCKALAEFLFDSQDAMVRIDMSEFMEK
HSVARLIGAPPGYVGYEEGGYLTELVRRRPYSLILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNL
GSHQIQELSGDDSPEVYTQMKAAVMGVVQAHFRPEFINRLDDIVVFHPLDKAQIKQIARIQLRGLEKRLAESELKLDLDD
RALELLGNVGFDPVYGARPLKRAIQSQLENALAQQILAGAFVSGDTVQVGVDGGKLVFSKV

Sequences:

>Translated_861_residues
MRMDKLTSRFQNALADAQSLAVGRDHTIIEPVHVFSVLLDQQGGSTRPLLVQAGVNVPLLRERLTEILEALPKVSGQTVN
VSPSNELSRLFHRTDKLAQQHGDQFMASEWFVLAVVDDSGGLGQALRAAGAEKKKIEAAIDKLRGGETVQTENAEEQRQA
LEKYTIDLTARAESGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLRSKRLLSL
DLGALIAGAKFRGEFEERLKGVLNDLAKNEGRVILFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYR
KYIEKDAALERRFQKVFVGEPTVEDTIAILRGLKEKYALHHGVEITDPAIVAAATLSNRYITDRQLPDKAIDLMDEAASR
IRMEIDSKPEELDRLERRLIQLKIQREMLKKEKDEASKQRLVDLERDIEVLDREFSDLEEVWRSEKAALQGATKIKESIE
QAKLDLEAAQRRQDYAKMSEIQYGVLPALEKQLVAASQAEQHDFTLVQEKVTAEEIAEVVSRWTGIPVSKMLEGERDKLL
RMEADLGRRVVGQDEAIKVVSDAVRRSRTGLSDPNRPSGSFLFLGPTGVGKTELCKALAEFLFDSQDAMVRIDMSEFMEK
HSVARLIGAPPGYVGYEEGGYLTELVRRRPYSLILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNL
GSHQIQELSGDDSPEVYTQMKAAVMGVVQAHFRPEFINRLDDIVVFHPLDKAQIKQIARIQLRGLEKRLAESELKLDLDD
RALELLGNVGFDPVYGARPLKRAIQSQLENALAQQILAGAFVSGDTVQVGVDGGKLVFSKV
>Mature_861_residues
MRMDKLTSRFQNALADAQSLAVGRDHTIIEPVHVFSVLLDQQGGSTRPLLVQAGVNVPLLRERLTEILEALPKVSGQTVN
VSPSNELSRLFHRTDKLAQQHGDQFMASEWFVLAVVDDSGGLGQALRAAGAEKKKIEAAIDKLRGGETVQTENAEEQRQA
LEKYTIDLTARAESGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLRSKRLLSL
DLGALIAGAKFRGEFEERLKGVLNDLAKNEGRVILFIDELHTMVGAGKAEGAMDAGNMLKPALARGELHCIGATTLDEYR
KYIEKDAALERRFQKVFVGEPTVEDTIAILRGLKEKYALHHGVEITDPAIVAAATLSNRYITDRQLPDKAIDLMDEAASR
IRMEIDSKPEELDRLERRLIQLKIQREMLKKEKDEASKQRLVDLERDIEVLDREFSDLEEVWRSEKAALQGATKIKESIE
QAKLDLEAAQRRQDYAKMSEIQYGVLPALEKQLVAASQAEQHDFTLVQEKVTAEEIAEVVSRWTGIPVSKMLEGERDKLL
RMEADLGRRVVGQDEAIKVVSDAVRRSRTGLSDPNRPSGSFLFLGPTGVGKTELCKALAEFLFDSQDAMVRIDMSEFMEK
HSVARLIGAPPGYVGYEEGGYLTELVRRRPYSLILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNL
GSHQIQELSGDDSPEVYTQMKAAVMGVVQAHFRPEFINRLDDIVVFHPLDKAQIKQIARIQLRGLEKRLAESELKLDLDD
RALELLGNVGFDPVYGARPLKRAIQSQLENALAQQILAGAFVSGDTVQVGVDGGKLVFSKV

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=317, Percent_Identity=37.2239747634069, Blast_Score=204, Evalue=4e-52,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=69.5449241540257, Blast_Score=1214, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=260, Percent_Identity=51.5384615384615, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6320464, Length=708, Percent_Identity=54.2372881355932, Blast_Score=768, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=868, Percent_Identity=42.741935483871, Blast_Score=681, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95650; Mature: 95650

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMDKLTSRFQNALADAQSLAVGRDHTIIEPVHVFSVLLDQQGGSTRPLLVQAGVNVPLL
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHH
RERLTEILEALPKVSGQTVNVSPSNELSRLFHRTDKLAQQHGDQFMASEWFVLAVVDDSG
HHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEEECCC
GLGQALRAAGAEKKKIEAAIDKLRGGETVQTENAEEQRQALEKYTIDLTARAESGKLDPV
CHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLRSKRLLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCHHC
DLGALIAGAKFRGEFEERLKGVLNDLAKNEGRVILFIDELHTMVGAGKAEGAMDAGNMLK
CHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCIGATTLDEYRKYIEKDAALERRFQKVFVGEPTVEDTIAILRGLKEKYALH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVEITDPAIVAAATLSNRYITDRQLPDKAIDLMDEAASRIRMEIDSKPEELDRLERRLI
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLKIQREMLKKEKDEASKQRLVDLERDIEVLDREFSDLEEVWRSEKAALQGATKIKESIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKLDLEAAQRRQDYAKMSEIQYGVLPALEKQLVAASQAEQHDFTLVQEKVTAEEIAEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SRWTGIPVSKMLEGERDKLLRMEADLGRRVVGQDEAIKVVSDAVRRSRTGLSDPNRPSGS
HHHCCCCHHHHHCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALAEFLFDSQDAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTELVRRRPYSLILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNL
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSHQIQELSGDDSPEVYTQMKAAVMGVVQAHFRPEFINRLDDIVVFHPLDKAQIKQIARI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHH
QLRGLEKRLAESELKLDLDDRALELLGNVGFDPVYGARPLKRAIQSQLENALAQQILAGA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FVSGDTVQVGVDGGKLVFSKV
HCCCCEEEEECCCCEEEEECC
>Mature Secondary Structure
MRMDKLTSRFQNALADAQSLAVGRDHTIIEPVHVFSVLLDQQGGSTRPLLVQAGVNVPLL
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHH
RERLTEILEALPKVSGQTVNVSPSNELSRLFHRTDKLAQQHGDQFMASEWFVLAVVDDSG
HHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEEEEEECCC
GLGQALRAAGAEKKKIEAAIDKLRGGETVQTENAEEQRQALEKYTIDLTARAESGKLDPV
CHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCCC
IGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLRSKRLLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHCCCHHC
DLGALIAGAKFRGEFEERLKGVLNDLAKNEGRVILFIDELHTMVGAGKAEGAMDAGNMLK
CHHHHHHCHHHHCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCCCCHHH
PALARGELHCIGATTLDEYRKYIEKDAALERRFQKVFVGEPTVEDTIAILRGLKEKYALH
HHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HGVEITDPAIVAAATLSNRYITDRQLPDKAIDLMDEAASRIRMEIDSKPEELDRLERRLI
CCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QLKIQREMLKKEKDEASKQRLVDLERDIEVLDREFSDLEEVWRSEKAALQGATKIKESIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAKLDLEAAQRRQDYAKMSEIQYGVLPALEKQLVAASQAEQHDFTLVQEKVTAEEIAEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SRWTGIPVSKMLEGERDKLLRMEADLGRRVVGQDEAIKVVSDAVRRSRTGLSDPNRPSGS
HHHCCCCHHHHHCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKALAEFLFDSQDAMVRIDMSEFMEKHSVARLIGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTELVRRRPYSLILLDEVEKAHSDVFNILLQVLDDGRLTDGQGRTVDFRNTVIVMTSNL
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSHQIQELSGDDSPEVYTQMKAAVMGVVQAHFRPEFINRLDDIVVFHPLDKAQIKQIARI
CCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHH
QLRGLEKRLAESELKLDLDDRALELLGNVGFDPVYGARPLKRAIQSQLENALAQQILAGA
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FVSGDTVQVGVDGGKLVFSKV
HCCCCEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]