Click here to switch to the map view.

The map label for this gene is mlaE [H]

Identifier: 182682267

GI number: 182682267

Start: 1938217

End: 1938966

Strand: Direct

Name: mlaE [H]

Synonym: XfasM23_1749

Alternate gene names: 182682267

Gene position: 1938217-1938966 (Clockwise)

Preceding gene: 182682266

Following gene: 182682268

Centisome position: 76.44

GC content: 54.27

Gene sequence:

>750_bases
ATGCCACTGGTATCTTCAATCAGCTCGCTAGGCCGCTCTGGTCTGTTTGCACTCACTGTATTACGCAGCTCGCTGCCCAC
ACGCGACTTCTTCGCCGAACTGATCCGTGAAATCTACAAGATTGGCGCGCGCTCGCTACCAATCATCGCCGTCGGAGGCG
CGTTCGTCGGCTTGGTACTGACCTTGCAAGGCTACCGCACACTCACTCTGTATGGCGCTTCCGACGCACTATCCACCCTC
CTAGGCCTATCACTGTATCGCGAACTTGCGCCCGTGCTGACTGCTCTGCTTTTCATCGGACGTGCGGGCAGCTCAGTGGC
GGCGGAACTGAGCCTGATGCGTGCGACCGATCAGATCAAGGCACTGGAGCTGATGGCCATCGACCCCATCGCTAAAGCAG
TGGCGCCACGCTTTTGGGCAGCGGTATTGACTGTACCTTTACTCACAGGTGTGTTTTGCTCACTGGCCATCTGCAGTGGT
TACTTCCAAGCAGTCCATGTGTTAGGCATAGACAACGGTTTATTCTGGTCAGGATTGAGCAACAGCGTGGACTTTTTGGA
GGATTTCGGCGTAGCAATGCTCAAATCAGCAATCTTTGGTGGCACCTCGGCTCTTGTGGCAGCTTACGTAGGCTTCCATG
CGCAACCAACCATTGAGGGCACTTCAATCGCAACCACGCGTGCAGTCGTCAACGCGTCATTGCTGGTATTGATGTTTAAC
TTCGTGCTATCAGCACTATTATTCCGATAA

Upstream 100 bases:

>100_bases
CCAGCAACGATGCGCTGGTATACCAGTTTATGCATGGGCAACCGGACGGGCCGATCCCATTCGATGCAGCACCACGCACA
TCTGAACGGAGTTCCGCCTA

Downstream 100 bases:

>100_bases
TCCGTGCTGCCTGCCCCGTCCTCCACATGAAAAAATCAGCGTTTTTCTACGCAAGATATTTAAAGAAATATGAAGTGAAA
ACCTGCTCTGATCCATCCAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPLVSSISSLGRSGLFALTVLRSSLPTRDFFAELIREIYKIGARSLPIIAVGGAFVGLVLTLQGYRTLTLYGASDALSTL
LGLSLYRELAPVLTALLFIGRAGSSVAAELSLMRATDQIKALELMAIDPIAKAVAPRFWAAVLTVPLLTGVFCSLAICSG
YFQAVHVLGIDNGLFWSGLSNSVDFLEDFGVAMLKSAIFGGTSALVAAYVGFHAQPTIEGTSIATTRAVVNASLLVLMFN
FVLSALLFR

Sequences:

>Translated_249_residues
MPLVSSISSLGRSGLFALTVLRSSLPTRDFFAELIREIYKIGARSLPIIAVGGAFVGLVLTLQGYRTLTLYGASDALSTL
LGLSLYRELAPVLTALLFIGRAGSSVAAELSLMRATDQIKALELMAIDPIAKAVAPRFWAAVLTVPLLTGVFCSLAICSG
YFQAVHVLGIDNGLFWSGLSNSVDFLEDFGVAMLKSAIFGGTSALVAAYVGFHAQPTIEGTSIATTRAVVNASLLVLMFN
FVLSALLFR
>Mature_248_residues
PLVSSISSLGRSGLFALTVLRSSLPTRDFFAELIREIYKIGARSLPIIAVGGAFVGLVLTLQGYRTLTLYGASDALSTLL
GLSLYRELAPVLTALLFIGRAGSSVAAELSLMRATDQIKALELMAIDPIAKAVAPRFWAAVLTVPLLTGVFCSLAICSGY
FQAVHVLGIDNGLFWSGLSNSVDFLEDFGVAMLKSAIFGGTSALVAAYVGFHAQPTIEGTSIATTRAVVNASLLVLMFNF
VLSALLFR

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=245, Percent_Identity=44.8979591836735, Blast_Score=215, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 26314; Mature: 26183

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLVSSISSLGRSGLFALTVLRSSLPTRDFFAELIREIYKIGARSLPIIAVGGAFVGLVL
CCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHH
TLQGYRTLTLYGASDALSTLLGLSLYRELAPVLTALLFIGRAGSSVAAELSLMRATDQIK
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALELMAIDPIAKAVAPRFWAAVLTVPLLTGVFCSLAICSGYFQAVHVLGIDNGLFWSGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCC
NSVDFLEDFGVAMLKSAIFGGTSALVAAYVGFHAQPTIEGTSIATTRAVVNASLLVLMFN
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FVLSALLFR
HHHHHHHCC
>Mature Secondary Structure 
PLVSSISSLGRSGLFALTVLRSSLPTRDFFAELIREIYKIGARSLPIIAVGGAFVGLVL
CHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHH
TLQGYRTLTLYGASDALSTLLGLSLYRELAPVLTALLFIGRAGSSVAAELSLMRATDQIK
HHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ALELMAIDPIAKAVAPRFWAAVLTVPLLTGVFCSLAICSGYFQAVHVLGIDNGLFWSGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCC
NSVDFLEDFGVAMLKSAIFGGTSALVAAYVGFHAQPTIEGTSIATTRAVVNASLLVLMFN
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
FVLSALLFR
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]