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The map label for this gene is mscM [H]

Identifier: 182682251

GI number: 182682251

Start: 1915924

End: 1917258

Strand: Reverse

Name: mscM [H]

Synonym: XfasM23_1733

Alternate gene names: 182682251

Gene position: 1917258-1915924 (Counterclockwise)

Preceding gene: 182682252

Following gene: 182682250

Centisome position: 75.61

GC content: 49.96

Gene sequence:

>1335_bases
ATGCTGTACATTGAAGGGGGCTGTGATTTTGGAGTGATGATGTTCAATGCCAGCATTGCGATTACCTGGACCCATTTGCA
TGATGTCTTGGCACCTTATCCTTGGGTCTACCGTGTATTAGCAACAATCATGTTGTTGTTGGCTGCTTGGTTTGCTAATT
GGGTGACGAAGCGGGTTTTGCTACGTGGTGTGCAGCGTGTGCTGCGTAGCAGTGTGCTTGCTGGCGAGGAGGGCGGCTAC
GGCATGCGTTTGGGTGTCATCCCGCGCTTGGCTAATGTGGTTCCAGCATTGGTGTTGTCTCTGGGAGTGGCCGCGGTGCC
TGATGTGCCACTTGTGGTGGTTGCAGTGGTGCGCAATGTTTGTACGGCTTTTATCGTATTGACCGTGGCATTGGCGTTGG
CTCGTTTGATGGATTTGGCCAATCAAGTGTATGAGCGCCGTCCCGATGCGCAGCACAAGCCAATCAAGAGTTATGTACAG
CTGCTGAAAATTGTGCTCGGTTTGTTTACGGCTGTACTGATGATTTCAGTATTGATAGACAAGTCGCCGCTGATCCTTCT
TTCTGGTCTGGGCGCCATGATGGCTGTGTTGATTTTGGTCTTTCAGGACACGTTGTTGTCGTTGGTCGCCAGTGTGACGA
TTAATTCCAACGATATGGTGCGGGTGGGTGATTGGATCGAAATGCCACAGCAGGGCGCTGATGGGGATGTGATTGATATT
GCGCTGCATACGATCAAGGTCCAGAACTGGGACAAAACGATTACTGCAATTCCAATCAAGCGATTGATCAGTGATTCGTT
CAAGAACTGGCGTGGAATGCATGAGAGTGGTGGGCGCCGGATCAAGCGGTCGCTATTTCTGGATCAGTGCAGTGTACGTT
TTCTTGATGATTCTGAAATTGCAAGGATGCGTGAGTTCAGCATATTGCGTGATTACATTGATGCAAAGTGCGAGAGTTTG
GCTGAGTGGAATGTGGCGCTGTCAAGTCAAGGTGTTGCTTCAGTCAATGCTCGCCGTATTACCAATCTTGGGACATTTCG
CGCTTATGTGGAGCATTATCTGCGTGCCAGCCCGCATGTGCACCAGGAGATGACGTTGTTAGTACGGCAATTGCAACCAA
CGGATCTTGGGCTGCCTTTGGAGATCTACTGTTTTACAAATAACACGGATTGGGTGGCTTACGAGCAAATTCAATCGGAC
ATTTTCGATCATCTGTTGGCGTTGTTGCCGCACTTTGGGTTGAGGGTTTTTCAGAGTTCCAGTGACACCATGTTGATGGA
AACGGCGCAACGTTTGAGTGCTATATCGGCTGCGTTAACGGGGCGTGCGCATTGA

Upstream 100 bases:

>100_bases
CCACCTCAGATGGTCCGTGGTGGTAAGACAAACGTGTGCTGCTGTTGAGATTATTTGTTTCTTGGTTCAATGCCAATGTG
TGGGGATACGTGGGTACCCC

Downstream 100 bases:

>100_bases
CGTGTGTATGCGCACGACAGGCATTAAGAAGGATGTGTCCACGATAGAGCGACTTGATATGATGTTTTACGACGCACTTA
TGTTGTTTAGCAGTGTATTG

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFANWVTKRVLLRGVQRVLRSSVLAGEEGGY
GMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALALARLMDLANQVYERRPDAQHKPIKSYVQ
LLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASVTINSNDMVRVGDWIEMPQQGADGDVIDI
ALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSVRFLDDSEIARMREFSILRDYIDAKCESL
AEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQPTDLGLPLEIYCFTNNTDWVAYEQIQSD
IFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH

Sequences:

>Translated_444_residues
MLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFANWVTKRVLLRGVQRVLRSSVLAGEEGGY
GMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALALARLMDLANQVYERRPDAQHKPIKSYVQ
LLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASVTINSNDMVRVGDWIEMPQQGADGDVIDI
ALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSVRFLDDSEIARMREFSILRDYIDAKCESL
AEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQPTDLGLPLEIYCFTNNTDWVAYEQIQSD
IFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH
>Mature_444_residues
MLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFANWVTKRVLLRGVQRVLRSSVLAGEEGGY
GMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNVCTAFIVLTVALALARLMDLANQVYERRPDAQHKPIKSYVQ
LLKIVLGLFTAVLMISVLIDKSPLILLSGLGAMMAVLILVFQDTLLSLVASVTINSNDMVRVGDWIEMPQQGADGDVIDI
ALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSVRFLDDSEIARMREFSILRDYIDAKCESL
AEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHVHQEMTLLVRQLQPTDLGLPLEIYCFTNNTDWVAYEQIQSD
IFDHLLALLPHFGLRVFQSSSDTMLMETAQRLSAISAALTGRAH

Specific function: Mechanosensitive channel of miniconductance that confers protection against mild hypoosmotic shock. Overexpression confers protection against severe shocks [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786791, Length=298, Percent_Identity=50.3355704697987, Blast_Score=320, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 49481; Mature: 49481

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFANWVTKRVL
CEEECCCCCEEEEEEECEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRGVQRVLRSSVLAGEEGGYGMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNV
HHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHH
CTAFIVLTVALALARLMDLANQVYERRPDAQHKPIKSYVQLLKIVLGLFTAVLMISVLID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KSPLILLSGLGAMMAVLILVFQDTLLSLVASVTINSNDMVRVGDWIEMPQQGADGDVIDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECHHHHCCCCCCCCCEEEE
ALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSVRFLDDSEI
EEHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHEECCCHHH
ARMREFSILRDYIDAKCESLAEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCH
HQEMTLLVRQLQPTDLGLPLEIYCFTNNTDWVAYEQIQSDIFDHLLALLPHFGLRVFQSS
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDTMLMETAQRLSAISAALTGRAH
CCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLYIEGGCDFGVMMFNASIAITWTHLHDVLAPYPWVYRVLATIMLLLAAWFANWVTKRVL
CEEECCCCCEEEEEEECEEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRGVQRVLRSSVLAGEEGGYGMRLGVIPRLANVVPALVLSLGVAAVPDVPLVVVAVVRNV
HHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHH
CTAFIVLTVALALARLMDLANQVYERRPDAQHKPIKSYVQLLKIVLGLFTAVLMISVLID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
KSPLILLSGLGAMMAVLILVFQDTLLSLVASVTINSNDMVRVGDWIEMPQQGADGDVIDI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEECHHHHCCCCCCCCCEEEE
ALHTIKVQNWDKTITAIPIKRLISDSFKNWRGMHESGGRRIKRSLFLDQCSVRFLDDSEI
EEHHEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHEECCCHHH
ARMREFSILRDYIDAKCESLAEWNVALSSQGVASVNARRITNLGTFRAYVEHYLRASPHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHHHCCCCH
HQEMTLLVRQLQPTDLGLPLEIYCFTNNTDWVAYEQIQSDIFDHLLALLPHFGLRVFQSS
HHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDTMLMETAQRLSAISAALTGRAH
CCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7813889 [H]