Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 182682132

GI number: 182682132

Start: 1785180

End: 1785866

Strand: Reverse

Name: nth [H]

Synonym: XfasM23_1610

Alternate gene names: 182682132

Gene position: 1785866-1785180 (Counterclockwise)

Preceding gene: 182682133

Following gene: 182682128

Centisome position: 70.43

GC content: 51.53

Gene sequence:

>687_bases
ATGACGATCGGATCTGCTGATAAACCCGTGATGCGTCGTGGCAGCGTAATGACCCGTGCGGAGATTCGCGAAGCATTTGT
GCGGTTACAAGAGATCAATCCACATCCAACCACGGAGCTGAAATACACCACTGCATTTGAGCTGTTGATTGCCGTGATCT
TGTCTGCACAGGCTACTGATATTGGAGTGAACAAGGCGACACGTCGACTGTATTCGCTTGCCAACACGCCACAGGCAATC
TTGGATTTGGGCGAGGATGCCTTGAAGCGTCATATTTCCACGATTGGCTTGTTTAATGCCAAGGCCAAGAATGTGATTGC
TACTTGCCGTATTCTGGTGGAGCAATACGGCGGTGCAGTTCCGCGGGATCGTGCCCTGCTGGAAGCATTACCCGGTGTAG
GGCGTAAAACGGCCAATGTGGTGCTTAACACAGCCTTTGGCGAACCTACCATCGCAGTAGACACACATATCTTCCGTGTT
GCCAACCGTACTGGTTTGGCAATTGGCAGCAATGTACGAGCCGTTGAGGATGCACTTCTCAAACGCATCCCGCAGGAGTT
TTTGAAGGATGCGCATCACTGGCTGATTCTGCACGGCCGCTATGTTTGCAAGGCACGCAAACCGAACTGCTCACAGTGTG
TGATTGCTGATTTATGTCGTTACAAGGACAAGACGCCAGCAGCTTAG

Upstream 100 bases:

>100_bases
CCGCACATTCATCCTCCACGGGGACATCGTCTGTCCTTCCCCCTTTGGCTGCGGAGATCCTCAAGCGTGCTAAGGCCAAG
GCGGCCGGACGCCAGGACAT

Downstream 100 bases:

>100_bases
AAGTTGCTATTACATTTTTTAAATCATTGGAAGGAAGACACATAAGGAATACACCAGGGGTGTTTATTTATCCTGGCTTG
CCCTTTATTCAATCCGTTGA

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTIGSADKPVMRRGSVMTRAEIREAFVRLQEINPHPTTELKYTTAFELLIAVILSAQATDIGVNKATRRLYSLANTPQAI
LDLGEDALKRHISTIGLFNAKAKNVIATCRILVEQYGGAVPRDRALLEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRV
ANRTGLAIGSNVRAVEDALLKRIPQEFLKDAHHWLILHGRYVCKARKPNCSQCVIADLCRYKDKTPAA

Sequences:

>Translated_228_residues
MTIGSADKPVMRRGSVMTRAEIREAFVRLQEINPHPTTELKYTTAFELLIAVILSAQATDIGVNKATRRLYSLANTPQAI
LDLGEDALKRHISTIGLFNAKAKNVIATCRILVEQYGGAVPRDRALLEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRV
ANRTGLAIGSNVRAVEDALLKRIPQEFLKDAHHWLILHGRYVCKARKPNCSQCVIADLCRYKDKTPAA
>Mature_227_residues
TIGSADKPVMRRGSVMTRAEIREAFVRLQEINPHPTTELKYTTAFELLIAVILSAQATDIGVNKATRRLYSLANTPQAIL
DLGEDALKRHISTIGLFNAKAKNVIATCRILVEQYGGAVPRDRALLEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVA
NRTGLAIGSNVRAVEDALLKRIPQEFLKDAHHWLILHGRYVCKARKPNCSQCVIADLCRYKDKTPAA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=32.7683615819209, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=67.3076923076923, Blast_Score=298, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45550361, Length=180, Percent_Identity=31.1111111111111, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25065; Mature: 24933

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGSADKPVMRRGSVMTRAEIREAFVRLQEINPHPTTELKYTTAFELLIAVILSAQATD
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCCC
IGVNKATRRLYSLANTPQAILDLGEDALKRHISTIGLFNAKAKNVIATCRILVEQYGGAV
CCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PRDRALLEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAIGSNVRAVEDALL
CCHHHHHHHCCCCCCCHHHEEEECCCCCCEEEEHHHHHHHHCCCCEEECCCHHHHHHHHH
KRIPQEFLKDAHHWLILHGRYVCKARKPNCSQCVIADLCRYKDKTPAA
HHHHHHHHHCCHHEEEEECEEEEECCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TIGSADKPVMRRGSVMTRAEIREAFVRLQEINPHPTTELKYTTAFELLIAVILSAQATD
CCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCCC
IGVNKATRRLYSLANTPQAILDLGEDALKRHISTIGLFNAKAKNVIATCRILVEQYGGAV
CCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
PRDRALLEALPGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRTGLAIGSNVRAVEDALL
CCHHHHHHHCCCCCCCHHHEEEECCCCCCEEEEHHHHHHHHCCCCEEECCCHHHHHHHHH
KRIPQEFLKDAHHWLILHGRYVCKARKPNCSQCVIADLCRYKDKTPAA
HHHHHHHHHCCHHEEEEECEEEEECCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]