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The map label for this gene is metE

Identifier: 182681924

GI number: 182681924

Start: 1548814

End: 1551090

Strand: Direct

Name: metE

Synonym: XfasM23_1392

Alternate gene names: 182681924

Gene position: 1548814-1551090 (Clockwise)

Preceding gene: 182681923

Following gene: 182681925

Centisome position: 61.08

GC content: 55.86

Gene sequence:

>2277_bases
ATGACAATTGTGACCAACCTGGGTTTCCCGCGCATCGGCGCACGACGCGAACTCAAGCGCGCCCTAGAAAGCCACTGGCG
TGGCGAGACCGATGCCACACAACTCCAACACACTGCACGCGAACTACGCGCACGGCACTGGCGACTACAACGTGATGCGG
GCGTGGACCTGCCACCCAGCAACGACTTCAGCCTCTATGACCACGTCCTAGATACCGCATTCCTGTTCGATGCCATTCCA
CAGCGCTACCGCGGCCTGGTAGATGCTGATCCTCTGGCCGGTTACTTCGCGATGGCGCGTGGCCGCCAAGCTGACAACAT
TGATCTGCATGCTCTGGAGATGACCAAATGGTTCGATACCAATTACCACTACCTCGTCCCAGAACTGCACCGTGACCAAC
ACTTCGCACTACGCGGTAACAAACCCATCGCCGAGTTTGAAGAAGCACTGGCACTGGGCATCACTACCAGACCGGTGCTC
CTTGGACCAGTCAGCTTTCTCCTGCTGTCTAAGACAGTGGATGGCAGCAACCGGCTCGACCTACTGGAACGTTTACTGCC
GGTCTACACCCAATTGCTGCGCCAATTGCAGGAAAGCGGTGCCGAATGGGTGCAAATTGATGAACCAACACTTGTGCTGG
ACCTGGATGCACAAACACAGCAAGCCTTCCGTAAAGCCTACGCCATACTCAATCAAGGACCACGCCCAAAATTGCTACTG
ACCAGCTACTTTGGTCCGCTCGGCGACAACCTCGAACTGGCCTTGCAACTGCCTGCCGATGGCTTACACATCGACTTAGT
GCGTGGCACCGAACAGTTGGATGCGGTCCTCAACACCTTGCCCGCGGGACGGGTACTCTCCGCCGGACTGGTGAATGGAC
GCAATATCTGGCGTACCGATCTGGATAACGCACTGACGATGGCACGCTACGCGCAGGGCCACGTCGGTGCGGATCGGTTA
TGGCTAGCACCGTCATGTTCACTACTGCATGTGCCTGTTGACCTGGAACAGGAAAAAAACCTGGACGCCGATGTACGCAA
TTGGCTCGCATTTGCCAAGCAAAAACTTAGCGAACTGCGCGTACTGGCTGACGCACTGGACAACAAGCCAGAAGCAGAAA
CAGCACTGACCCAAACACGCCAAGCATTGGAGGCACGGCGCCAATCACCCAAAGTCCATCGCCCCGACGTGGCACAGCGT
CTGGCCGCACTCACACCGGACACCACCCGGCGCAACACGGCCTACCCACAACGCAGCCAGGCACAACAACACACACTCAA
CCTACCCGCTTACCCAACCACCACCATTGGCTCCTTCCCACAGACGCTAGAAGTCCGTGAGGCCCGCGCGCAGTTCAAAT
CCGGCAAGTTGTCTGAAAGCGACTATGAGGCGTTTTTGAAAGCGGAAACCGAACGTTGCGTACGTACCCAGGAAGAGATC
GGGTTGGACGTCTTGGTCCATGGCGAATTCGAGCGTAACGACATGGTCGAATACTTCGGGGAGCAATTGGACGGCTTCAT
CTTCACCAAGCTCGGTTGGGTACAGAGTTACGGATCACGCTGTGTCAAACCACCCATCATTTATGGCGATGTAGTGCGTC
CAGCACCGATGACCGTCACCTGGTCGGCTTACGCACAATCACTCACCGACAAACCAATGAAAGGGATGCTCACCGGTCCG
GTGACAATGCTGCAATGGTCGTTTGTACGCGATGATCAGGAACGCGCGCAGACATGCCGGCAGATCGCACTAGCCCTTCG
CGACGAAGTCCAGGATTTAGAAAAAGCCGGAATCAAGGTGATTCAAATCGACGAACCAGCCATCCGCGAAGGGCTACCGC
TGCGCCGTGGTGAATGGGCAGACTATCTGAACTGGGCTGTGGAGAGCTTCCGCATTGCCTCTTCAAACGTCCATGACACT
ACACAGATTCATACCCACATGTGCTACTCGGAATTCAACGACATCATTGAAGCCGTGGCTGCATTGGATGCAGACGTGAT
CTCGATTGAAACATCCCGTTCACGCATGGAATTACTGGATGCCTTCGTGAAATTCCGCTATCCAAACGCCATCGGCCCAG
GGGTGTACGATATTCACTCCCCGCGTGTCCCACAGGAAGAGGAAATGGTGCTGCTATTGAAAAAAGCACGCGCAGTACTT
CCCCCGGAACAATTGTGGGTCAACCCGGATTGCGGCCTGAAGACGCGCGGTTGGAAAGAAACACGTGCTGCATTGCAAAC
AATGGTGCATGCCGCGCAGCGGCTACGTGCCGAATAA

Upstream 100 bases:

>100_bases
CGCTAGCGGAGCAGAAGGCCAATACCCTGGAATCGACAACAAGCAGGATGCACCTTTATTTCGCTCTTCACCGCCCTTAC
AAGTAATGAGAACACCCACT

Downstream 100 bases:

>100_bases
GCACAGACCATCCGTATACAATCGCTGGGGCGTTCAACCGCCCCAGCTCGTACTGGAGCACCCTGTATGACCTCCCCAAC
TCCTACTCGCCAACCTCTAC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MTIVTNLGFPRIGARRELKRALESHWRGETDATQLQHTARELRARHWRLQRDAGVDLPPSNDFSLYDHVLDTAFLFDAIP
QRYRGLVDADPLAGYFAMARGRQADNIDLHALEMTKWFDTNYHYLVPELHRDQHFALRGNKPIAEFEEALALGITTRPVL
LGPVSFLLLSKTVDGSNRLDLLERLLPVYTQLLRQLQESGAEWVQIDEPTLVLDLDAQTQQAFRKAYAILNQGPRPKLLL
TSYFGPLGDNLELALQLPADGLHIDLVRGTEQLDAVLNTLPAGRVLSAGLVNGRNIWRTDLDNALTMARYAQGHVGADRL
WLAPSCSLLHVPVDLEQEKNLDADVRNWLAFAKQKLSELRVLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQR
LAALTPDTTRRNTAYPQRSQAQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEI
GLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGP
VTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDT
TQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVKFRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVL
PPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE

Sequences:

>Translated_758_residues
MTIVTNLGFPRIGARRELKRALESHWRGETDATQLQHTARELRARHWRLQRDAGVDLPPSNDFSLYDHVLDTAFLFDAIP
QRYRGLVDADPLAGYFAMARGRQADNIDLHALEMTKWFDTNYHYLVPELHRDQHFALRGNKPIAEFEEALALGITTRPVL
LGPVSFLLLSKTVDGSNRLDLLERLLPVYTQLLRQLQESGAEWVQIDEPTLVLDLDAQTQQAFRKAYAILNQGPRPKLLL
TSYFGPLGDNLELALQLPADGLHIDLVRGTEQLDAVLNTLPAGRVLSAGLVNGRNIWRTDLDNALTMARYAQGHVGADRL
WLAPSCSLLHVPVDLEQEKNLDADVRNWLAFAKQKLSELRVLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQR
LAALTPDTTRRNTAYPQRSQAQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEI
GLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGP
VTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDT
TQIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVKFRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVL
PPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE
>Mature_757_residues
TIVTNLGFPRIGARRELKRALESHWRGETDATQLQHTARELRARHWRLQRDAGVDLPPSNDFSLYDHVLDTAFLFDAIPQ
RYRGLVDADPLAGYFAMARGRQADNIDLHALEMTKWFDTNYHYLVPELHRDQHFALRGNKPIAEFEEALALGITTRPVLL
GPVSFLLLSKTVDGSNRLDLLERLLPVYTQLLRQLQESGAEWVQIDEPTLVLDLDAQTQQAFRKAYAILNQGPRPKLLLT
SYFGPLGDNLELALQLPADGLHIDLVRGTEQLDAVLNTLPAGRVLSAGLVNGRNIWRTDLDNALTMARYAQGHVGADRLW
LAPSCSLLHVPVDLEQEKNLDADVRNWLAFAKQKLSELRVLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQRL
AALTPDTTRRNTAYPQRSQAQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEIG
LDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGPV
TMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKVIQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDTT
QIHTHMCYSEFNDIIEAVAALDADVISIETSRSRMELLDAFVKFRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVLP
PEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family

Homologues:

Organism=Escherichia coli, GI2367304, Length=764, Percent_Identity=56.8062827225131, Blast_Score=837, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=766, Percent_Identity=49.4778067885117, Blast_Score=749, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METE_XYLF2 (B2I621)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001830084.1
- ProteinModelPortal:   B2I621
- SMR:   B2I621
- GeneID:   6202326
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_1392
- HOGENOM:   HBG287495
- OMA:   RFGWVQS
- ProtClustDB:   PRK05222
- HAMAP:   MF_00172
- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629
- PIRSF:   PIRSF000382
- TIGRFAMs:   TIGR01371

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2

EC number: =2.1.1.14

Molecular weight: Translated: 85749; Mature: 85617

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVTNLGFPRIGARRELKRALESHWRGETDATQLQHTARELRARHWRLQRDAGVDLPPS
CEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDFSLYDHVLDTAFLFDAIPQRYRGLVDADPLAGYFAMARGRQADNIDLHALEMTKWFDT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHCC
NYHYLVPELHRDQHFALRGNKPIAEFEEALALGITTRPVLLGPVSFLLLSKTVDGSNRLD
CCEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHH
LLERLLPVYTQLLRQLQESGAEWVQIDEPTLVLDLDAQTQQAFRKAYAILNQGPRPKLLL
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEE
TSYFGPLGDNLELALQLPADGLHIDLVRGTEQLDAVLNTLPAGRVLSAGLVNGRNIWRTD
EECCCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHH
LDNALTMARYAQGHVGADRLWLAPSCSLLHVPVDLEQEKNLDADVRNWLAFAKQKLSELR
HHHHHHHHHHHCCCCCCCCEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQRLAALTPDTTRRNTAYPQRSQ
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHH
AQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPPIIYGDVVRPAPMTVT
CCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEE
WSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKV
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVA
EEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALDADVISIETSRSRMELLDAFVKFRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVL
HCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCC
PPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE
CHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIVTNLGFPRIGARRELKRALESHWRGETDATQLQHTARELRARHWRLQRDAGVDLPPS
EEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
NDFSLYDHVLDTAFLFDAIPQRYRGLVDADPLAGYFAMARGRQADNIDLHALEMTKWFDT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEHHHHHCC
NYHYLVPELHRDQHFALRGNKPIAEFEEALALGITTRPVLLGPVSFLLLSKTVDGSNRLD
CCEEECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHH
LLERLLPVYTQLLRQLQESGAEWVQIDEPTLVLDLDAQTQQAFRKAYAILNQGPRPKLLL
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHCCCCCCCEEE
TSYFGPLGDNLELALQLPADGLHIDLVRGTEQLDAVLNTLPAGRVLSAGLVNGRNIWRTD
EECCCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHH
LDNALTMARYAQGHVGADRLWLAPSCSLLHVPVDLEQEKNLDADVRNWLAFAKQKLSELR
HHHHHHHHHHHCCCCCCCCEEECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VLADALDNKPEAETALTQTRQALEARRQSPKVHRPDVAQRLAALTPDTTRRNTAYPQRSQ
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHH
AQQHTLNLPAYPTTTIGSFPQTLEVREARAQFKSGKLSESDYEAFLKAETERCVRTQEEI
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GLDVLVHGEFERNDMVEYFGEQLDGFIFTKLGWVQSYGSRCVKPPIIYGDVVRPAPMTVT
CCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEE
WSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQERAQTCRQIALALRDEVQDLEKAGIKV
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
IQIDEPAIREGLPLRRGEWADYLNWAVESFRIASSNVHDTTQIHTHMCYSEFNDIIEAVA
EEECCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ALDADVISIETSRSRMELLDAFVKFRYPNAIGPGVYDIHSPRVPQEEEMVLLLKKARAVL
HCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHCC
PPEQLWVNPDCGLKTRGWKETRAALQTMVHAAQRLRAE
CHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA