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The map label for this gene is glnG [H]

Identifier: 182681627

GI number: 182681627

Start: 1228630

End: 1230057

Strand: Direct

Name: glnG [H]

Synonym: XfasM23_1085

Alternate gene names: 182681627

Gene position: 1228630-1230057 (Clockwise)

Preceding gene: 182681626

Following gene: 182681628

Centisome position: 48.45

GC content: 53.08

Gene sequence:

>1428_bases
ATGGTTGAGATTGATGCGACTTCCCCACGCATCTGGGTGGTTGACGACGATCGCTCGGTACGCTTCGTGTTATCTACTGC
ACTGCGTGATGCAGGCTATATCGTGGAAGGTTTCGAGAGTACTTGCGCAGCGTTGCAAGCCCTAGCAGCCTCCCCAATCC
CAGACTTATTGTTCACCGACGTACGCATGCCTGGTGATGATGGTTTGATTTTGCTGGACAAGCTCAAAGCACGCCATCCG
CACCTTCCAGTAATTGTTATGTCTGCCTACACCGATGTGGCTAGCACTGCCGGTGCATTTCGCAGTGGTGCACATGAATT
CTTGTCCAAGCCATTTGATCTAGACGATGCGGTTGCGTTGGCAGCTCGTGTGTTGCCACATACACAAGGATCTCATGGGG
GATCACCCATATTGCCTGCTGCCAACAGTAATCCCGTCTTGATCGGCAGCACTCCGGTGATGCGTGCTTTGTTCCGTGCA
ATCGGACGGTTGGCGCAGGCACCACTCTCGGTGCTGATTAACGGTGAAACAGGTACTGGCAAAGAGCTCGTTGCACGTGC
GTTACACAACGAATCTCCGCGTGCGCAGGCGCCGTTCGTTGCGCTGAATGCTGCGGCGATTCCAACCGAGCTTTTGGAGA
GCGAGTTGTTCGGTCACGAGGCCGGCGCGTTTACAGGAGCGACCAAACGTCATATTGGCCGCTTTGAGCAAGCCGATGGC
GGCACTTTATTTTTGGATGAAATCGGCGATATGCCGTTGTCTTTGCAGACGAGACTGTTACGTGTACTGGCTGAAAATGA
ATTCTTCCGGGTCGGGGGCCGCGAACTGATCCGTGTCAATGTCCGTGTGATTACTGCGACCCATCAGGATCTTGATATGT
TGGTCGAGCAGGGGCGTTTTCGTTCCGATCTCTTGCACCGTTTAGATGTAGTGCGCTTGCAACTGCCATCGTTACGTGAG
CGGCGTGATGATGTGGCACAGTTGGCCGAGAATTTTTTGGTAATGGCTGGGCACAAGCTGGATATCTCAGTTAAACGTTT
GTCTGCCGATGCGCTAGAGATACTGCGTCGCTACGATTGGCCAGGTAATGTCCGTGAGCTCGAAAATATTTGCTGGCGAT
TAGCTGCTTTGGTGAACACTGAGACAATTGATGCCAATGATGTTGAAGCTGTGTTAACACATGGCAAGCGCAAGCGTTGT
AACACTGAAAGCGATTGGGATATCCATTTAGCCAAATGGGCGGAAGACCGCTTAGCTGCAGGCGCAACTGCTGTGCATGC
CGAAGCTCGCAAATGTTTGGACCGAACGCTACTGGATGTGGCATTGCGTTTGACGCAGGGCCGTCGTGCCGAAGCAGCTG
CACGTCTTGGTCTGGGTCGTAACACGTTGACCCGCAAGCTTGGCTCAAGTCAACGTCGTAGGAGGTAA

Upstream 100 bases:

>100_bases
GAGCATCATGGAATGCTGACTTTCCGCTCACAGCCAGGACAGACCATATTTATCCTGTCGCTACCTCAAATGATGATCGA
GTCTGACAAGGATCTGCACT

Downstream 100 bases:

>100_bases
GCCATCAGAATGCTGTTGTGTTCAAGATGGAGTTGCTTCAGAAAACGGAAAATAAAGCACGCTAATCCGGTTGCAATAGT
CGTAGTGGTCTAAACTTGCC

Product: Fis family nitrogen metabolism transcriptional regulator NtrC

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MVEIDATSPRIWVVDDDRSVRFVLSTALRDAGYIVEGFESTCAALQALAASPIPDLLFTDVRMPGDDGLILLDKLKARHP
HLPVIVMSAYTDVASTAGAFRSGAHEFLSKPFDLDDAVALAARVLPHTQGSHGGSPILPAANSNPVLIGSTPVMRALFRA
IGRLAQAPLSVLINGETGTGKELVARALHNESPRAQAPFVALNAAAIPTELLESELFGHEAGAFTGATKRHIGRFEQADG
GTLFLDEIGDMPLSLQTRLLRVLAENEFFRVGGRELIRVNVRVITATHQDLDMLVEQGRFRSDLLHRLDVVRLQLPSLRE
RRDDVAQLAENFLVMAGHKLDISVKRLSADALEILRRYDWPGNVRELENICWRLAALVNTETIDANDVEAVLTHGKRKRC
NTESDWDIHLAKWAEDRLAAGATAVHAEARKCLDRTLLDVALRLTQGRRAEAAARLGLGRNTLTRKLGSSQRRRR

Sequences:

>Translated_475_residues
MVEIDATSPRIWVVDDDRSVRFVLSTALRDAGYIVEGFESTCAALQALAASPIPDLLFTDVRMPGDDGLILLDKLKARHP
HLPVIVMSAYTDVASTAGAFRSGAHEFLSKPFDLDDAVALAARVLPHTQGSHGGSPILPAANSNPVLIGSTPVMRALFRA
IGRLAQAPLSVLINGETGTGKELVARALHNESPRAQAPFVALNAAAIPTELLESELFGHEAGAFTGATKRHIGRFEQADG
GTLFLDEIGDMPLSLQTRLLRVLAENEFFRVGGRELIRVNVRVITATHQDLDMLVEQGRFRSDLLHRLDVVRLQLPSLRE
RRDDVAQLAENFLVMAGHKLDISVKRLSADALEILRRYDWPGNVRELENICWRLAALVNTETIDANDVEAVLTHGKRKRC
NTESDWDIHLAKWAEDRLAAGATAVHAEARKCLDRTLLDVALRLTQGRRAEAAARLGLGRNTLTRKLGSSQRRRR
>Mature_475_residues
MVEIDATSPRIWVVDDDRSVRFVLSTALRDAGYIVEGFESTCAALQALAASPIPDLLFTDVRMPGDDGLILLDKLKARHP
HLPVIVMSAYTDVASTAGAFRSGAHEFLSKPFDLDDAVALAARVLPHTQGSHGGSPILPAANSNPVLIGSTPVMRALFRA
IGRLAQAPLSVLINGETGTGKELVARALHNESPRAQAPFVALNAAAIPTELLESELFGHEAGAFTGATKRHIGRFEQADG
GTLFLDEIGDMPLSLQTRLLRVLAENEFFRVGGRELIRVNVRVITATHQDLDMLVEQGRFRSDLLHRLDVVRLQLPSLRE
RRDDVAQLAENFLVMAGHKLDISVKRLSADALEILRRYDWPGNVRELENICWRLAALVNTETIDANDVEAVLTHGKRKRC
NTESDWDIHLAKWAEDRLAAGATAVHAEARKCLDRTLLDVALRLTQGRRAEAAARLGLGRNTLTRKLGSSQRRRR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=460, Percent_Identity=51.7391304347826, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1790437, Length=457, Percent_Identity=41.7943107221007, Blast_Score=319, Evalue=3e-88,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=38.8888888888889, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1788905, Length=391, Percent_Identity=39.8976982097187, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87082117, Length=238, Percent_Identity=51.6806722689076, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789233, Length=344, Percent_Identity=41.2790697674419, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789087, Length=324, Percent_Identity=42.2839506172839, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI1786524, Length=248, Percent_Identity=49.5967741935484, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=52.8138528138528, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87081872, Length=228, Percent_Identity=49.1228070175439, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1787583, Length=330, Percent_Identity=35.4545454545455, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=33.2278481012658, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1789828, Length=300, Percent_Identity=30.6666666666667, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790860, Length=128, Percent_Identity=28.125, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52158; Mature: 52158

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEIDATSPRIWVVDDDRSVRFVLSTALRDAGYIVEGFESTCAALQALAASPIPDLLFTD
CEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
VRMPGDDGLILLDKLKARHPHLPVIVMSAYTDVASTAGAFRSGAHEFLSKPFDLDDAVAL
CCCCCCCCEEEEEHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AARVLPHTQGSHGGSPILPAANSNPVLIGSTPVMRALFRAIGRLAQAPLSVLINGETGTG
HHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCC
KELVARALHNESPRAQAPFVALNAAAIPTELLESELFGHEAGAFTGATKRHIGRFEQADG
HHHHHHHHHCCCCCCCCCEEEEECHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCC
GTLFLDEIGDMPLSLQTRLLRVLAENEFFRVGGRELIRVNVRVITATHQDLDMLVEQGRF
CEEEHHHHCCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEEEEEEECHHHHHHHHHCCCH
RSDLLHRLDVVRLQLPSLRERRDDVAQLAENFLVMAGHKLDISVKRLSADALEILRRYDW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHHHCCC
PGNVRELENICWRLAALVNTETIDANDVEAVLTHGKRKRCNTESDWDIHLAKWAEDRLAA
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHCCCCCCCCCEEHHHHHHHHHHC
GATAVHAEARKCLDRTLLDVALRLTQGRRAEAAARLGLGRNTLTRKLGSSQRRRR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MVEIDATSPRIWVVDDDRSVRFVLSTALRDAGYIVEGFESTCAALQALAASPIPDLLFTD
CEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHC
VRMPGDDGLILLDKLKARHPHLPVIVMSAYTDVASTAGAFRSGAHEFLSKPFDLDDAVAL
CCCCCCCCEEEEEHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
AARVLPHTQGSHGGSPILPAANSNPVLIGSTPVMRALFRAIGRLAQAPLSVLINGETGTG
HHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCEEEEEECCCCCC
KELVARALHNESPRAQAPFVALNAAAIPTELLESELFGHEAGAFTGATKRHIGRFEQADG
HHHHHHHHHCCCCCCCCCEEEEECHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCC
GTLFLDEIGDMPLSLQTRLLRVLAENEFFRVGGRELIRVNVRVITATHQDLDMLVEQGRF
CEEEHHHHCCCCHHHHHHHHHHHHCCCEEECCCCEEEEEEEEEEEECHHHHHHHHHCCCH
RSDLLHRLDVVRLQLPSLRERRDDVAQLAENFLVMAGHKLDISVKRLSADALEILRRYDW
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCEEEEEHHHHHHHHHHHHHHCCC
PGNVRELENICWRLAALVNTETIDANDVEAVLTHGKRKRCNTESDWDIHLAKWAEDRLAA
CCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHCCCCCCCCCEEHHHHHHHHHHC
GATAVHAEARKCLDRTLLDVALRLTQGRRAEAAARLGLGRNTLTRKLGSSQRRRR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]