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The map label for this gene is ntrB [H]

Identifier: 182681626

GI number: 182681626

Start: 1227588

End: 1228637

Strand: Direct

Name: ntrB [H]

Synonym: XfasM23_1084

Alternate gene names: 182681626

Gene position: 1227588-1228637 (Clockwise)

Preceding gene: 182681625

Following gene: 182681627

Centisome position: 48.41

GC content: 52.0

Gene sequence:

>1050_bases
ATGCCGGTTCCACCACCATCATTAGAAAATTTATGTACCCCAGTAGCATGGGGGGATGCTGAAGGACGTATCCAAGGTGT
TAATGCAGCTTTCGCATATTGGCTTGGAGTCAGTGAAAAACGTCTACAACAGCAAACTCTGGAAGCACTTGAGCATCAGG
GAAATGCCCTGTCAAGCTTCCTGAACAATGATGAACACAATGTACTGCATTTGCATCGTTTTGCACTCGGGATACCCGGT
GGGACGCCGTCTTTCGCTGAGGGTTGGTTAACTAGGTTGGAAGCAGGCGCTTGGTTACTCGAAGCGCATCCAATCAAAGA
GTGTTCCGCATTGGACACGCTTCAAGCATTACCTAATGCGCTTAGCGCTGCGTTCAAAGGGCTTGCGCATGAGTTGCGCA
ATCCGCTCTCTGGCCTCAAAGGTGCTGCACAACTGTTAGCACGCCGTGTCAAACACCGCGACCAGGAGGAATGCGATTTG
GTTGAACTGATTGGTGCCGAGATCGAACGGCTGAACACCCTGTTGGAGCGCCTATTGTTTTCGGCACCGGCGCGTCCGCA
TGCGCAGATTAATATTCATACTGTGCTTGAGCGCGTCCTGCCTTTAGCTGAAACTGAAGCCGGTTGCTCAGTGATCCTAC
AGCGCGACTATGACCTCGGTATTCCGAACATCCTTGGAGATGCTGATAGGTTGACCCAGGCAGTATGGAACTTAGTACGC
AATGCGATCCAAGCCGGTGCCAACCGTATCATCCTGCGTACTCGCGTTGATCATGGGCAGCGCATCCGTGATCGTATCAA
CGTGATATCGCTACGTTTGGAGGTCATTGATGATGGCGATGGTGTTCCCGAAGCATTGGCCGAACACGTATTTCTACCAT
TGGTCAGCAGTCGTGCAGAGGGGAGCGGTCTTGGCTTGCCCCTGGCTCAGCAGGTCGCGCACGAGCATCATGGAATGCTG
ACTTTCCGCTCACAGCCAGGACAGACCATATTTATCCTGTCGCTACCTCAAATGATGATCGAGTCTGACAAGGATCTGCA
CTATGGTTGA

Upstream 100 bases:

>100_bases
ATTAAGTTATTGATTCTATTTTACTTTATGACTAAAATTGATGCTTTTCTAGAACTGCATACTGTTCAGACATATTGGGC
ACTACATTGGTGCAATGAAG

Downstream 100 bases:

>100_bases
GATTGATGCGACTTCCCCACGCATCTGGGTGGTTGACGACGATCGCTCGGTACGCTTCGTGTTATCTACTGCACTGCGTG
ATGCAGGCTATATCGTGGAA

Product: nitrogen-specific signal transduction histidine kinase NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MPVPPPSLENLCTPVAWGDAEGRIQGVNAAFAYWLGVSEKRLQQQTLEALEHQGNALSSFLNNDEHNVLHLHRFALGIPG
GTPSFAEGWLTRLEAGAWLLEAHPIKECSALDTLQALPNALSAAFKGLAHELRNPLSGLKGAAQLLARRVKHRDQEECDL
VELIGAEIERLNTLLERLLFSAPARPHAQINIHTVLERVLPLAETEAGCSVILQRDYDLGIPNILGDADRLTQAVWNLVR
NAIQAGANRIILRTRVDHGQRIRDRINVISLRLEVIDDGDGVPEALAEHVFLPLVSSRAEGSGLGLPLAQQVAHEHHGML
TFRSQPGQTIFILSLPQMMIESDKDLHYG

Sequences:

>Translated_349_residues
MPVPPPSLENLCTPVAWGDAEGRIQGVNAAFAYWLGVSEKRLQQQTLEALEHQGNALSSFLNNDEHNVLHLHRFALGIPG
GTPSFAEGWLTRLEAGAWLLEAHPIKECSALDTLQALPNALSAAFKGLAHELRNPLSGLKGAAQLLARRVKHRDQEECDL
VELIGAEIERLNTLLERLLFSAPARPHAQINIHTVLERVLPLAETEAGCSVILQRDYDLGIPNILGDADRLTQAVWNLVR
NAIQAGANRIILRTRVDHGQRIRDRINVISLRLEVIDDGDGVPEALAEHVFLPLVSSRAEGSGLGLPLAQQVAHEHHGML
TFRSQPGQTIFILSLPQMMIESDKDLHYG
>Mature_348_residues
PVPPPSLENLCTPVAWGDAEGRIQGVNAAFAYWLGVSEKRLQQQTLEALEHQGNALSSFLNNDEHNVLHLHRFALGIPGG
TPSFAEGWLTRLEAGAWLLEAHPIKECSALDTLQALPNALSAAFKGLAHELRNPLSGLKGAAQLLARRVKHRDQEECDLV
ELIGAEIERLNTLLERLLFSAPARPHAQINIHTVLERVLPLAETEAGCSVILQRDYDLGIPNILGDADRLTQAVWNLVRN
AIQAGANRIILRTRVDHGQRIRDRINVISLRLEVIDDGDGVPEALAEHVFLPLVSSRAEGSGLGLPLAQQVAHEHHGMLT
FRSQPGQTIFILSLPQMMIESDKDLHYG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=258, Percent_Identity=38.3720930232558, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790436, Length=222, Percent_Identity=32.4324324324324, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788549, Length=223, Percent_Identity=30.4932735426009, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081816, Length=231, Percent_Identity=30.7359307359307, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI87082128, Length=228, Percent_Identity=25.8771929824561, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38345; Mature: 38214

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPPPSLENLCTPVAWGDAEGRIQGVNAAFAYWLGVSEKRLQQQTLEALEHQGNALSSF
CCCCCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
LNNDEHNVLHLHRFALGIPGGTPSFAEGWLTRLEAGAWLLEAHPIKECSALDTLQALPNA
HCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
LSAAFKGLAHELRNPLSGLKGAAQLLARRVKHRDQEECDLVELIGAEIERLNTLLERLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SAPARPHAQINIHTVLERVLPLAETEAGCSVILQRDYDLGIPNILGDADRLTQAVWNLVR
HCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHH
NAIQAGANRIILRTRVDHGQRIRDRINVISLRLEVIDDGDGVPEALAEHVFLPLVSSRAE
HHHHHCCCEEEEEECCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GSGLGLPLAQQVAHEHHGMLTFRSQPGQTIFILSLPQMMIESDKDLHYG
CCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEECCHHHHCCCCCCCCC
>Mature Secondary Structure 
PVPPPSLENLCTPVAWGDAEGRIQGVNAAFAYWLGVSEKRLQQQTLEALEHQGNALSSF
CCCCCCHHHHCCCCCCCCCCCCEECCHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHH
LNNDEHNVLHLHRFALGIPGGTPSFAEGWLTRLEAGAWLLEAHPIKECSALDTLQALPNA
HCCCCCCCEEHHHHHHCCCCCCCHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
LSAAFKGLAHELRNPLSGLKGAAQLLARRVKHRDQEECDLVELIGAEIERLNTLLERLLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SAPARPHAQINIHTVLERVLPLAETEAGCSVILQRDYDLGIPNILGDADRLTQAVWNLVR
HCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHHH
NAIQAGANRIILRTRVDHGQRIRDRINVISLRLEVIDDGDGVPEALAEHVFLPLVSSRAE
HHHHHCCCEEEEEECCCCHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GSGLGLPLAQQVAHEHHGMLTFRSQPGQTIFILSLPQMMIESDKDLHYG
CCCCCCHHHHHHHHHHCCEEEEECCCCCEEEEEECCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]