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The map label for this gene is gidB

Identifier: 182681460

GI number: 182681460

Start: 1083048

End: 1083686

Strand: Reverse

Name: gidB

Synonym: XfasM23_0911

Alternate gene names: 182681460

Gene position: 1083686-1083048 (Counterclockwise)

Preceding gene: 182681461

Following gene: 182681454

Centisome position: 42.74

GC content: 52.74

Gene sequence:

>639_bases
ATGAATGATTCTTCTCTTTCCCCAGAAGTGACAGCAGACTTGGAATATGGTCTGGATATCTTGGAACTGGACCGTGCCTA
TGTTGTTCCGCTGTTGGCCTATTTGACACTGCTCATACGCTGGAACCGTACCTACAATTTAACTGCGATCCGTGATCCAC
GGGAGATGGTGGTCCGTCATTTGCTCGATTCGTTGGCGATACAGCGTTATGTGACTGTCGGCCGCCTTGCTGACCTTGGC
TCTGGTCCGGGATTGCCTGGGATTCCGCTAGCGATTAGTTGCCCGTCTCTACAGGTGACATTGGTGGAAAGCAATGGCAA
GAAGGCGCGCTTTCTACGGGAGGTGGTGCGTCAACTTGGTTTGAGTAATGTGGGTGTGTCTGAGGTGCGTGCCGAGGCGT
TGGACGAAGCTCTCATGTATGAGCATCTCACTGCACGTGCCTTGGATACATTAAACGGCATTGTTACGGTTGGCGGGCAT
CTGCTCAAATCTGAGGGGACGTTGCTGGCGATGAAGGGTGCCTATCCTCATGAGGAGATCGCCATGTTGCCACCCCATTG
GGTTGTCGAAGCAGTACACCGATTGCAAGTCCCCAAGCTAACCGGAGAGCGGCATTTGGTGATAGTACGGAAGAGATAG

Upstream 100 bases:

>100_bases
ACTGTCATCCATAGCTTCCTCAATGATCTCTTTCCGTGTGCTCGATATAGGATCTCCATGTTTTTACTGGGGCATGTTCT
AAAACGAAGCCAATTTACCG

Downstream 100 bases:

>100_bases
TGTTGTCTCAGCTCTTCCACATAGTGCCTTTGCGACTTCGACCACGTGTTCTGCAGTGATATTAAAATGTTTGTACAACA
CATCCGCTGGGGCTGATGCA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNDSSLSPEVTADLEYGLDILELDRAYVVPLLAYLTLLIRWNRTYNLTAIRDPREMVVRHLLDSLAIQRYVTVGRLADLG
SGPGLPGIPLAISCPSLQVTLVESNGKKARFLREVVRQLGLSNVGVSEVRAEALDEALMYEHLTARALDTLNGIVTVGGH
LLKSEGTLLAMKGAYPHEEIAMLPPHWVVEAVHRLQVPKLTGERHLVIVRKR

Sequences:

>Translated_212_residues
MNDSSLSPEVTADLEYGLDILELDRAYVVPLLAYLTLLIRWNRTYNLTAIRDPREMVVRHLLDSLAIQRYVTVGRLADLG
SGPGLPGIPLAISCPSLQVTLVESNGKKARFLREVVRQLGLSNVGVSEVRAEALDEALMYEHLTARALDTLNGIVTVGGH
LLKSEGTLLAMKGAYPHEEIAMLPPHWVVEAVHRLQVPKLTGERHLVIVRKR
>Mature_212_residues
MNDSSLSPEVTADLEYGLDILELDRAYVVPLLAYLTLLIRWNRTYNLTAIRDPREMVVRHLLDSLAIQRYVTVGRLADLG
SGPGLPGIPLAISCPSLQVTLVESNGKKARFLREVVRQLGLSNVGVSEVRAEALDEALMYEHLTARALDTLNGIVTVGGH
LLKSEGTLLAMKGAYPHEEIAMLPPHWVVEAVHRLQVPKLTGERHLVIVRKR

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=180, Percent_Identity=45.5555555555556, Blast_Score=174, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_XYLF2 (B2I4Q1)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829620.1
- ProteinModelPortal:   B2I4Q1
- SMR:   B2I4Q1
- GeneID:   6202831
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0911
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 23469; Mature: 23469

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSSLSPEVTADLEYGLDILELDRAYVVPLLAYLTLLIRWNRTYNLTAIRDPREMVVRH
CCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
LLDSLAIQRYVTVGRLADLGSGPGLPGIPLAISCPSLQVTLVESNGKKARFLREVVRQLG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHC
LSNVGVSEVRAEALDEALMYEHLTARALDTLNGIVTVGGHLLKSEGTLLAMKGAYPHEEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCE
AMLPPHWVVEAVHRLQVPKLTGERHLVIVRKR
EECCCHHHHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MNDSSLSPEVTADLEYGLDILELDRAYVVPLLAYLTLLIRWNRTYNLTAIRDPREMVVRH
CCCCCCCCCEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHH
LLDSLAIQRYVTVGRLADLGSGPGLPGIPLAISCPSLQVTLVESNGKKARFLREVVRQLG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCCCHHHHHHHHHHHHC
LSNVGVSEVRAEALDEALMYEHLTARALDTLNGIVTVGGHLLKSEGTLLAMKGAYPHEEI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEECCCCCCCE
AMLPPHWVVEAVHRLQVPKLTGERHLVIVRKR
EECCCHHHHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA