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The map label for this gene is lpdG [H]

Identifier: 182681353

GI number: 182681353

Start: 943892

End: 945328

Strand: Reverse

Name: lpdG [H]

Synonym: XfasM23_0800

Alternate gene names: 182681353

Gene position: 945328-943892 (Counterclockwise)

Preceding gene: 182681354

Following gene: 182681350

Centisome position: 37.28

GC content: 52.89

Gene sequence:

>1437_bases
ATGAGTGAACATTACGACGTCGTTGTTATCGGAGCCGGTCCAGCTGGCTATCACGCTGCAATCCGCGCCGCCCAACTGGG
TCTGAAGGTCGCTTGCGTCGATGCAGCACTCGGCAAGGACGGTAAGCCAGCGCTTGGAGGCACGTGTCTACGCGTGGGAT
GCATCCCTTCCAAAGCACTGCTGGACTCTTCCCGCCAGTACTGGAACATGGGACATCTATTCAACGAACATGGCATCAGT
TTCAAAGATGCCAAGATGGATATTGCCACGATGATCGCCCGCAAAGACAAAATCGTGAAACAGTTCACCGGTGGCATTGC
GATGCTCTTCAAAGCAAACAAAATCACTCCCTACTATGGATTTGCCCAATTGCAACCTGGCAACGTGGTCAAAGTGAAAC
AACACGAGGGTAAGAACATCGAACTGAAAGGCGCCAACGTGATCCTGGCATCAGGCTCGGATTCCGTTGAACTGCCATTT
GCAACGTTCGACGGTGAAACGATTGTCGACAATATCGGTGCCCTGGACTTTACAAAAGTCCCAAAACGCTTGGCGGTGAT
CGGTGCCGGAGTCATCGGTCTGGAATTAGGCAGCGTATGGAAACGCCTAGGTGCGGAAGTCACTATTCTTGAAGCAATGC
CAAACTTTCTAGCTGCAGTTGACGCCGATGTCGCCAAAACCGCCGCCAAGGAATTCAAGCAACAGGGGCTGGACATTAAA
CTCGGCGCTAAGGTCTCCCGAACCGAAATTTCAAGTAAAAACAACAAAAAAGAAGTCATCGTTACCTACACTGACGCCTC
CGGTAAGCAGACACTCACTGTCGACAAACTATTAGTGGCTGTCGGGCGCAAGGCCGCAACCAAGGGATTGCTTGCAGATG
GCACGGGGGTACAGCTCAACGAACGTGGCCAGATCGTGGTGGACGAACACTGTCATACCGGTGTCGACGGTGTCTGGGCC
ATTGGCGACTGTGTACGCGGCCCGATGTTGGCTCACAAAGGCTTCGAAGAGGGGATCGCAGTAGCTGAATTGATCGCGGG
CCTGCCAGGCCATGTGAACTTTGAAACCATCCCATGGGTCATCTACACAGAGCCGGAAATCGCCTGGGTTGGTAAGACTG
AGCAAGAACTCAAAGCAGACAACATTCCTTACAAAGCTGGATCCTTCCCGTTCGCCGCAGTCGGTCGCGCCGTTGCGATG
GCCGAGCCATCTGGTTTTGCGAAAGTCCTAGCACATGCAGAAACTGATCGCGTGTTAGGTTTACATCTGATCGGTGTGAA
CGTCTCCGAATTGGTACATGAAGGGGTATTGGCTATGGAATTTAGTGGCTCAGCTGACGATCTCGCACGGATCTGCCACG
CCCACCCAACCTTATCGGAAGCAATCCACGACGCAGCTATGGCGGTCAGCAAGCGCTCAATCCATAAAGCAAACTGA

Upstream 100 bases:

>100_bases
AGTAAAAAAGAATGCCAATAATACATAGACAAAATAAAAAGCGGTCAAGATAACTACTCATTGATTGACGTTGCAATCAA
ACAAGAACAGGAAAACAGCA

Downstream 100 bases:

>100_bases
TCTCCCTCAAGTAGCTCCGCATTAGGCATCGTTCTAAGGACTCCAGAACGATGCCTTTTTGTGCTTTTTACTCACATCCG
TGTCCTATAGCGTCCTCCTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSEHYDVVVIGAGPAGYHAAIRAAQLGLKVACVDAALGKDGKPALGGTCLRVGCIPSKALLDSSRQYWNMGHLFNEHGIS
FKDAKMDIATMIARKDKIVKQFTGGIAMLFKANKITPYYGFAQLQPGNVVKVKQHEGKNIELKGANVILASGSDSVELPF
ATFDGETIVDNIGALDFTKVPKRLAVIGAGVIGLELGSVWKRLGAEVTILEAMPNFLAAVDADVAKTAAKEFKQQGLDIK
LGAKVSRTEISSKNNKKEVIVTYTDASGKQTLTVDKLLVAVGRKAATKGLLADGTGVQLNERGQIVVDEHCHTGVDGVWA
IGDCVRGPMLAHKGFEEGIAVAELIAGLPGHVNFETIPWVIYTEPEIAWVGKTEQELKADNIPYKAGSFPFAAVGRAVAM
AEPSGFAKVLAHAETDRVLGLHLIGVNVSELVHEGVLAMEFSGSADDLARICHAHPTLSEAIHDAAMAVSKRSIHKAN

Sequences:

>Translated_478_residues
MSEHYDVVVIGAGPAGYHAAIRAAQLGLKVACVDAALGKDGKPALGGTCLRVGCIPSKALLDSSRQYWNMGHLFNEHGIS
FKDAKMDIATMIARKDKIVKQFTGGIAMLFKANKITPYYGFAQLQPGNVVKVKQHEGKNIELKGANVILASGSDSVELPF
ATFDGETIVDNIGALDFTKVPKRLAVIGAGVIGLELGSVWKRLGAEVTILEAMPNFLAAVDADVAKTAAKEFKQQGLDIK
LGAKVSRTEISSKNNKKEVIVTYTDASGKQTLTVDKLLVAVGRKAATKGLLADGTGVQLNERGQIVVDEHCHTGVDGVWA
IGDCVRGPMLAHKGFEEGIAVAELIAGLPGHVNFETIPWVIYTEPEIAWVGKTEQELKADNIPYKAGSFPFAAVGRAVAM
AEPSGFAKVLAHAETDRVLGLHLIGVNVSELVHEGVLAMEFSGSADDLARICHAHPTLSEAIHDAAMAVSKRSIHKAN
>Mature_477_residues
SEHYDVVVIGAGPAGYHAAIRAAQLGLKVACVDAALGKDGKPALGGTCLRVGCIPSKALLDSSRQYWNMGHLFNEHGISF
KDAKMDIATMIARKDKIVKQFTGGIAMLFKANKITPYYGFAQLQPGNVVKVKQHEGKNIELKGANVILASGSDSVELPFA
TFDGETIVDNIGALDFTKVPKRLAVIGAGVIGLELGSVWKRLGAEVTILEAMPNFLAAVDADVAKTAAKEFKQQGLDIKL
GAKVSRTEISSKNNKKEVIVTYTDASGKQTLTVDKLLVAVGRKAATKGLLADGTGVQLNERGQIVVDEHCHTGVDGVWAI
GDCVRGPMLAHKGFEEGIAVAELIAGLPGHVNFETIPWVIYTEPEIAWVGKTEQELKADNIPYKAGSFPFAAVGRAVAMA
EPSGFAKVLAHAETDRVLGLHLIGVNVSELVHEGVLAMEFSGSADDLARICHAHPTLSEAIHDAAMAVSKRSIHKAN

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=44.0425531914894, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI50301238, Length=473, Percent_Identity=24.3128964059197, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI22035672, Length=475, Percent_Identity=26.7368421052632, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI148277065, Length=498, Percent_Identity=24.0963855421687, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI33519430, Length=498, Percent_Identity=24.0963855421687, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI33519428, Length=498, Percent_Identity=24.0963855421687, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI33519426, Length=498, Percent_Identity=24.0963855421687, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI148277071, Length=498, Percent_Identity=24.0963855421687, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI291045266, Length=498, Percent_Identity=22.6907630522088, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI291045268, Length=490, Percent_Identity=21.8367346938776, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=39.6551724137931, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=29.7413793103448, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=29.0254237288136, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789915, Length=467, Percent_Identity=25.6959314775161, Blast_Score=139, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=470, Percent_Identity=45.7446808510638, Blast_Score=401, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17557007, Length=495, Percent_Identity=26.8686868686869, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=360, Percent_Identity=25, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71983419, Length=379, Percent_Identity=25.5936675461741, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71982272, Length=503, Percent_Identity=23.0616302186879, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=148, Percent_Identity=31.0810810810811, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=45.2282157676349, Blast_Score=400, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=29.4117647058824, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6325166, Length=476, Percent_Identity=25.8403361344538, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=46.7948717948718, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24640549, Length=493, Percent_Identity=26.5720081135903, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24640553, Length=493, Percent_Identity=26.5720081135903, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI17737741, Length=494, Percent_Identity=26.7206477732794, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24640551, Length=493, Percent_Identity=26.5720081135903, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50710; Mature: 50579

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHYDVVVIGAGPAGYHAAIRAAQLGLKVACVDAALGKDGKPALGGTCLRVGCIPSKAL
CCCCEEEEEEECCCCCHHHHHHHHHHCEEEEEEEHHHCCCCCCCCCCEEEEEECCCCHHH
LDSSRQYWNMGHLFNEHGISFKDAKMDIATMIARKDKIVKQFTGGIAMLFKANKITPYYG
HHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
FAQLQPGNVVKVKQHEGKNIELKGANVILASGSDSVELPFATFDGETIVDNIGALDFTKV
EEEECCCCEEEEEECCCCEEEEECCEEEEECCCCCEEEEEEECCCCHHHHCCCCCCHHHH
PKRLAVIGAGVIGLELGSVWKRLGAEVTILEAMPNFLAAVDADVAKTAAKEFKQQGLDIK
HHHHHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LGAKVSRTEISSKNNKKEVIVTYTDASGKQTLTVDKLLVAVGRKAATKGLLADGTGVQLN
ECCEEEHHHCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCHHHHCCEEECCCCCEEC
ERGQIVVDEHCHTGVDGVWAIGDCVRGPMLAHKGFEEGIAVAELIAGLPGHVNFETIPWV
CCCCEEEECCCCCCCCCEEEHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEECEE
IYTEPEIAWVGKTEQELKADNIPYKAGSFPFAAVGRAVAMAEPSGFAKVLAHAETDRVLG
EEECCCEEEECCCHHHHHCCCCCCCCCCCCHHHHCCEEEECCCCCHHHHHHHCCCCCEEE
LHLIGVNVSELVHEGVLAMEFSGSADDLARICHAHPTLSEAIHDAAMAVSKRSIHKAN
EEEECCCHHHHHHCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SEHYDVVVIGAGPAGYHAAIRAAQLGLKVACVDAALGKDGKPALGGTCLRVGCIPSKAL
CCCEEEEEEECCCCCHHHHHHHHHHCEEEEEEEHHHCCCCCCCCCCEEEEEECCCCHHH
LDSSRQYWNMGHLFNEHGISFKDAKMDIATMIARKDKIVKQFTGGIAMLFKANKITPYYG
HHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCC
FAQLQPGNVVKVKQHEGKNIELKGANVILASGSDSVELPFATFDGETIVDNIGALDFTKV
EEEECCCCEEEEEECCCCEEEEECCEEEEECCCCCEEEEEEECCCCHHHHCCCCCCHHHH
PKRLAVIGAGVIGLELGSVWKRLGAEVTILEAMPNFLAAVDADVAKTAAKEFKQQGLDIK
HHHHHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LGAKVSRTEISSKNNKKEVIVTYTDASGKQTLTVDKLLVAVGRKAATKGLLADGTGVQLN
ECCEEEHHHCCCCCCCCEEEEEEECCCCCEEEHHHHHHHHHCCHHHHCCEEECCCCCEEC
ERGQIVVDEHCHTGVDGVWAIGDCVRGPMLAHKGFEEGIAVAELIAGLPGHVNFETIPWV
CCCCEEEECCCCCCCCCEEEHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEECEE
IYTEPEIAWVGKTEQELKADNIPYKAGSFPFAAVGRAVAMAEPSGFAKVLAHAETDRVLG
EEECCCEEEECCCHHHHHCCCCCCCCCCCCHHHHCCEEEECCCCCHHHHHHHCCCCCEEE
LHLIGVNVSELVHEGVLAMEFSGSADDLARICHAHPTLSEAIHDAAMAVSKRSIHKAN
EEEECCCHHHHHHCCEEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]