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The map label for this gene is cysI

Identifier: 182681304

GI number: 182681304

Start: 885893

End: 887599

Strand: Reverse

Name: cysI

Synonym: XfasM23_0751

Alternate gene names: 182681304

Gene position: 887599-885893 (Counterclockwise)

Preceding gene: 182681305

Following gene: 182681303

Centisome position: 35.0

GC content: 56.94

Gene sequence:

>1707_bases
ATGAGTTCCTCCTCCATTGAAGACATCAAGGGCAAGAGCCACCGCCTGCGGGGGTCTCTGTTGGAGAGTCTCGCAAATCC
AACCACCGGGGCGCTCGGCGAAAGCGACCAAACGCTGATTAAGTACCACGGCAGCTACCAGCAGGATGATCGTGATCTAC
GTGAGGAGCGCCGTCGGCAAAAACTCGAACCTGCTTACCAATTCATGATCCGCACCCGAACACCTGGCGGCGTCATCACG
CCGCAGCAGTGGCTGCAGTTGGATGCCATCGCCACGCGCTACGCCAACCACTCATTGCGCGTCACAACACGTCAGGCATT
CCAGTTCCACGGAGTCATCAAACGCGAATTGAAAACAACAATGCAGGCAATCAACGCGGCATTGATCGATACACTTGCGG
CCTGCGGGGATGTCAATCGCAACGTACAGGTCGCTGCCAATCCATTGCTCTCGCGTGCCCATGCAGACCTTTATACCGAC
GCAGCGCACCTGTCTGAGCATCTGCTACCCAACACACGCGCTTACTACGAAATTTGGCTGGATGAAAAAAAGGTCGCTGG
TGCCGGTGAGGAAGAGGAACCAATCTACGGCCCGCACTATCTACCGCGTAAATTCAAAATCGGTTTTGCCGCACCACCAA
TCAACGATGTCGATGTATTCGCCAACGACCTGGGGTTTATCGCAGTCATCGTTGACAACACTTTGCTTGGCTATAACGTC
ACTATCGGCGGCGGAATGGGGACAACGCATGGTGACCCCGACACGTGGCCTCGCGTCGGCAACATCATCGGCTTCATCAC
CCGCGCCGATCTGATCACAATCAGCACGGCCATCGTGACCACGCAACGCGACTTCGGCAATCGAACGCTACGCAAGCGCG
CCCGCTTCAAATACACCATTGATGACCGCGGTCTAGATTGCATCGTCGGCGAGATCCAGCAGCGCGCCGGCATCACACTG
CAACCAGCACGACCGTTTGTATTCGAACACAATGGGGACCGTTACGGGTGGATTGAAGGTGAGGACGGGCACTGGCATCT
GACCTTATCGCTACCAGCTGGCCGCATCGCCGATACCGAAGGCAGCACGCTGCTGAGTGGCTTCCGAGAAATCGCACAAC
TTGGCATCGGCGAATTCCGCATGACTCCGAATCAGAATGTCGTGATTGCTGGCATCTCTCCCGGACAGCGCGCCGCAATC
GACGCACTGGTCACCCAGTACGGTCTGGATACCGGTAACCGTGCGCCCACAGCCCTCGGACGTCACGCCATGGCGTGTGT
CGCACTCCCGACCTGCGGTCTAGCGATGGCCGAAGCAGAACGTTATCTGCCGGACTTCAACGCGAAGCTACAGCCCATCC
TGGAAAAATATGGACTTGCTGAAGCACCCATTCTGCTGCGCATTTCCGGGTGTCCAAACGGCTGCTCCCGCCCCTATCTC
GCCGAAATTGCGTTGGTCGGTAAAGCCCCTGGACGCTATAACCTCATGCTCGGAGGCGATCAACGCGGCCAGCGCCTCAA
CACGCTGTATCGGGAAAACATCACCGAAACTGAAATCCTTGCGGCACTAGAACCATTGCTGGGCCGCTATCAACAGAAAC
GCCTTCCGAGCGAAGGCTTTGGCGATTTTCTACATCGCACCGGCATCATCGCACTTCCCCCATATCCCACCCACCGACAC
GTGATTTCCTCAACGCTGCAAGCATGA

Upstream 100 bases:

>100_bases
GTATCGTGGCCATGCGTCGCTACAATCCCAACGACACATGGCGACCATGTCGCCCCAAACATCCTTGTCCATCCTCCTCC
ATCCGGGATCGCCTGCACCC

Downstream 100 bases:

>100_bases
CCGTACTTCCCGCCTTACCTCCTCTGGACGATCTGGAAACGCTCAACGTCCACCTAGAAACGCTGAGTGCTGAAAATCGC
GTGTGCTGGGCACTGGAGCA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MSSSSIEDIKGKSHRLRGSLLESLANPTTGALGESDQTLIKYHGSYQQDDRDLREERRRQKLEPAYQFMIRTRTPGGVIT
PQQWLQLDAIATRYANHSLRVTTRQAFQFHGVIKRELKTTMQAINAALIDTLAACGDVNRNVQVAANPLLSRAHADLYTD
AAHLSEHLLPNTRAYYEIWLDEKKVAGAGEEEEPIYGPHYLPRKFKIGFAAPPINDVDVFANDLGFIAVIVDNTLLGYNV
TIGGGMGTTHGDPDTWPRVGNIIGFITRADLITISTAIVTTQRDFGNRTLRKRARFKYTIDDRGLDCIVGEIQQRAGITL
QPARPFVFEHNGDRYGWIEGEDGHWHLTLSLPAGRIADTEGSTLLSGFREIAQLGIGEFRMTPNQNVVIAGISPGQRAAI
DALVTQYGLDTGNRAPTALGRHAMACVALPTCGLAMAEAERYLPDFNAKLQPILEKYGLAEAPILLRISGCPNGCSRPYL
AEIALVGKAPGRYNLMLGGDQRGQRLNTLYRENITETEILAALEPLLGRYQQKRLPSEGFGDFLHRTGIIALPPYPTHRH
VISSTLQA

Sequences:

>Translated_568_residues
MSSSSIEDIKGKSHRLRGSLLESLANPTTGALGESDQTLIKYHGSYQQDDRDLREERRRQKLEPAYQFMIRTRTPGGVIT
PQQWLQLDAIATRYANHSLRVTTRQAFQFHGVIKRELKTTMQAINAALIDTLAACGDVNRNVQVAANPLLSRAHADLYTD
AAHLSEHLLPNTRAYYEIWLDEKKVAGAGEEEEPIYGPHYLPRKFKIGFAAPPINDVDVFANDLGFIAVIVDNTLLGYNV
TIGGGMGTTHGDPDTWPRVGNIIGFITRADLITISTAIVTTQRDFGNRTLRKRARFKYTIDDRGLDCIVGEIQQRAGITL
QPARPFVFEHNGDRYGWIEGEDGHWHLTLSLPAGRIADTEGSTLLSGFREIAQLGIGEFRMTPNQNVVIAGISPGQRAAI
DALVTQYGLDTGNRAPTALGRHAMACVALPTCGLAMAEAERYLPDFNAKLQPILEKYGLAEAPILLRISGCPNGCSRPYL
AEIALVGKAPGRYNLMLGGDQRGQRLNTLYRENITETEILAALEPLLGRYQQKRLPSEGFGDFLHRTGIIALPPYPTHRH
VISSTLQA
>Mature_567_residues
SSSSIEDIKGKSHRLRGSLLESLANPTTGALGESDQTLIKYHGSYQQDDRDLREERRRQKLEPAYQFMIRTRTPGGVITP
QQWLQLDAIATRYANHSLRVTTRQAFQFHGVIKRELKTTMQAINAALIDTLAACGDVNRNVQVAANPLLSRAHADLYTDA
AHLSEHLLPNTRAYYEIWLDEKKVAGAGEEEEPIYGPHYLPRKFKIGFAAPPINDVDVFANDLGFIAVIVDNTLLGYNVT
IGGGMGTTHGDPDTWPRVGNIIGFITRADLITISTAIVTTQRDFGNRTLRKRARFKYTIDDRGLDCIVGEIQQRAGITLQ
PARPFVFEHNGDRYGWIEGEDGHWHLTLSLPAGRIADTEGSTLLSGFREIAQLGIGEFRMTPNQNVVIAGISPGQRAAID
ALVTQYGLDTGNRAPTALGRHAMACVALPTCGLAMAEAERYLPDFNAKLQPILEKYGLAEAPILLRISGCPNGCSRPYLA
EIALVGKAPGRYNLMLGGDQRGQRLNTLYRENITETEILAALEPLLGRYQQKRLPSEGFGDFLHRTGIIALPPYPTHRHV
ISSTLQA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=548, Percent_Identity=46.8978102189781, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322597, Length=576, Percent_Identity=45.6597222222222, Blast_Score=524, Evalue=1e-149,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_XYLF2 (B2IA31)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829464.1
- GeneID:   6202438
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0751
- HOGENOM:   HBG626671
- OMA:   TRQAFQM
- ProtClustDB:   PRK13504
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 62682; Mature: 62551

Theoretical pI: Translated: 7.68; Mature: 7.68

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSSIEDIKGKSHRLRGSLLESLANPTTGALGESDQTLIKYHGSYQQDDRDLREERRRQ
CCCCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
KLEPAYQFMIRTRTPGGVITPQQWLQLDAIATRYANHSLRVTTRQAFQFHGVIKRELKTT
HCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
MQAINAALIDTLAACGDVNRNVQVAANPLLSRAHADLYTDAAHLSEHLLPNTRAYYEIWL
HHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
DEKKVAGAGEEEEPIYGPHYLPRKFKIGFAAPPINDVDVFANDLGFIAVIVDNTLLGYNV
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEECCEEEEEEE
TIGGGMGTTHGDPDTWPRVGNIIGFITRADLITISTAIVTTQRDFGNRTLRKRARFKYTI
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEECHHHCCHHHHHHHCEEEEE
DDRGLDCIVGEIQQRAGITLQPARPFVFEHNGDRYGWIEGEDGHWHLTLSLPAGRIADTE
CCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCC
GSTLLSGFREIAQLGIGEFRMTPNQNVVIAGISPGQRAAIDALVTQYGLDTGNRAPTALG
CHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHH
RHAMACVALPTCGLAMAEAERYLPDFNAKLQPILEKYGLAEAPILLRISGCPNGCSRPYL
HHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCE
AEIALVGKAPGRYNLMLGGDQRGQRLNTLYRENITETEILAALEPLLGRYQQKRLPSEGF
EEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
GDFLHRTGIIALPPYPTHRHVISSTLQA
HHHHHHCCEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SSSSIEDIKGKSHRLRGSLLESLANPTTGALGESDQTLIKYHGSYQQDDRDLREERRRQ
CCCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
KLEPAYQFMIRTRTPGGVITPQQWLQLDAIATRYANHSLRVTTRQAFQFHGVIKRELKTT
HCCHHHHEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHH
MQAINAALIDTLAACGDVNRNVQVAANPLLSRAHADLYTDAAHLSEHLLPNTRAYYEIWL
HHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
DEKKVAGAGEEEEPIYGPHYLPRKFKIGFAAPPINDVDVFANDLGFIAVIVDNTLLGYNV
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHCCCCEEEEEECCEEEEEEE
TIGGGMGTTHGDPDTWPRVGNIIGFITRADLITISTAIVTTQRDFGNRTLRKRARFKYTI
EECCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEEECHHHCCHHHHHHHCEEEEE
DDRGLDCIVGEIQQRAGITLQPARPFVFEHNGDRYGWIEGEDGHWHLTLSLPAGRIADTE
CCCCCHHHHHHHHHHCCCEEECCCCEEEECCCCEEEEEECCCCEEEEEEECCCCCCCCCC
GSTLLSGFREIAQLGIGEFRMTPNQNVVIAGISPGQRAAIDALVTQYGLDTGNRAPTALG
CHHHHHHHHHHHHCCCCCEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHH
RHAMACVALPTCGLAMAEAERYLPDFNAKLQPILEKYGLAEAPILLRISGCPNGCSRPYL
HHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCE
AEIALVGKAPGRYNLMLGGDQRGQRLNTLYRENITETEILAALEPLLGRYQQKRLPSEGF
EEEEEEECCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
GDFLHRTGIIALPPYPTHRHVISSTLQA
HHHHHHCCEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA