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The map label for this gene is zraS [H]

Identifier: 182681264

GI number: 182681264

Start: 838212

End: 839681

Strand: Direct

Name: zraS [H]

Synonym: XfasM23_0709

Alternate gene names: 182681264

Gene position: 838212-839681 (Clockwise)

Preceding gene: 182681263

Following gene: 182681265

Centisome position: 33.06

GC content: 53.27

Gene sequence:

>1470_bases
TTGTTGAAGATTCTGCTGATAGGGGATTCGCTAGAGGACGCCAAGTTACTGTTCAACACGTTGCGTGAGGCAGGGTTGGA
TGGGAGTTTTGAGCGTGTAGAGAGCGAATCAGCGCTGCGTGAGGCGTTGTATCTTTTCGCGCCGGATATTGTGCTTTCCG
ATCTGAGCATGTCTGGTTTCCACGGTTATCAAGCGTTACGCGTGGTGCGCGAGGTGGGCAATACGCCGTTTATCTTCGTC
TCCAGCAAGATGGGTGAAGATATTGCAGTCGAGGCGTTGCAGGAAGGTGCTAACGACTACATCATCAAACAAAATTTGGT
GCGCTTGCCAAGTGCGGTGACGCGCGCGGTCCGTGAGTCACGGGCTGAGCTAGAGCGTCAGCACGTAGAGTCGGAGTTAA
TGCGTGCGCAAAGGCTGGAGAGCTTGGCGATGTTAGCGGCTGGTTTCAGTCACGACCTGCGTAATATTTTGCAGCCCTTG
CTCATTGTCCCTGATCTGCTGGCGGGACGGACCGATGATCCGCAGTTGCATCAATTGGTGAGCATCGTTGCCGAGTGTGG
ACGGCGTGGTCACGAGATGGCCGAATCGATTCTTTCTTTCGTGAGAGGTTCCAACAAGCCGCGTGAGCGGGTTTTATTGG
CGGATCTATTCCAGACAGTGCAGTTATTGTTGCGTAGTAGCGTGCCTGATTGTATTGCTCTGCGTATTGAAGAGTGTGAT
GAATATCTCAGCGTGGAGGCCAATTATACCGAGCTACAGCAGTGCTTACTTAATTTGGCCTTGAATGCGATTCATGCCAT
GCCAACCGGGGGGTGCTTGACCTTGGTTGCACAGCGCCATGATGGCGACCGTATTTGTATTAGCGTGACTGACACCGGTA
TCGGTATGAGTGAAGAAACCCGGGCACGGTTGTTCAGTCCATTCTTCACGACCAAGTCCGATGGTACCGGTTTGGGACTG
ATTTCTTGCAAACGCATTGCTGAGAGTTATGGTGGCTTGATTCAGGTCTGCAGTGAATTGGGAGTCGGTACGCGTTTTGA
TCTGATCTTGCCGGCACGTGCACCGGCTTCAAGGGTATCAGAGGAAAATGTGCCTGTAGTGCTTGGTCATGGCCAGCGTA
TCTTGATCGTTGATGGAGAGGCGACGCGTCTGTCGTTGCTGGGAAATGCGTTGGCCAGTCAGGGCTATCAGCCGCAATTG
GCTCCCGATGGTGGTGCGGCACTAAAACTGCTGCGTCACCATGCAGAGCCGGATTTAGTCATTATCGACAGTGACATCCT
GTTATTGTCGGCGGTCTGTCTGCTCCCGACGATGCAGGAGTTGGGCTACAGGGGGCCGGCCATCGTACTTGAGGATGCTG
GTCAGCCTTTGCGCCGTGAGCATTTCTCGAAGGACTTGGCGGTTCACATGTTGCGAAAACCCTTAGAGATGGGGCGTGTA
TTTCGAGCCGTGGCGTATGCACTGACATAA

Upstream 100 bases:

>100_bases
TGCTGTCGAGATGTTTGGCCTATATCCTGCGTGTTCGAGTGACATGCGTCCGGACACTATTTGGGAATAATCCATGTCGC
AATAGGCTGAGAAACTGGGT

Downstream 100 bases:

>100_bases
CGTATGACGTAGTGGGCGGAGTTTTGACAGAACACTTAGTGTGCAGCACGAGGGCAGCAATAACGCTGCATTCATGCCCA
TGACGGAGGTTGACGTTGTC

Product: response regulator receiver sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MLKILLIGDSLEDAKLLFNTLREAGLDGSFERVESESALREALYLFAPDIVLSDLSMSGFHGYQALRVVREVGNTPFIFV
SSKMGEDIAVEALQEGANDYIIKQNLVRLPSAVTRAVRESRAELERQHVESELMRAQRLESLAMLAAGFSHDLRNILQPL
LIVPDLLAGRTDDPQLHQLVSIVAECGRRGHEMAESILSFVRGSNKPRERVLLADLFQTVQLLLRSSVPDCIALRIEECD
EYLSVEANYTELQQCLLNLALNAIHAMPTGGCLTLVAQRHDGDRICISVTDTGIGMSEETRARLFSPFFTTKSDGTGLGL
ISCKRIAESYGGLIQVCSELGVGTRFDLILPARAPASRVSEENVPVVLGHGQRILIVDGEATRLSLLGNALASQGYQPQL
APDGGAALKLLRHHAEPDLVIIDSDILLLSAVCLLPTMQELGYRGPAIVLEDAGQPLRREHFSKDLAVHMLRKPLEMGRV
FRAVAYALT

Sequences:

>Translated_489_residues
MLKILLIGDSLEDAKLLFNTLREAGLDGSFERVESESALREALYLFAPDIVLSDLSMSGFHGYQALRVVREVGNTPFIFV
SSKMGEDIAVEALQEGANDYIIKQNLVRLPSAVTRAVRESRAELERQHVESELMRAQRLESLAMLAAGFSHDLRNILQPL
LIVPDLLAGRTDDPQLHQLVSIVAECGRRGHEMAESILSFVRGSNKPRERVLLADLFQTVQLLLRSSVPDCIALRIEECD
EYLSVEANYTELQQCLLNLALNAIHAMPTGGCLTLVAQRHDGDRICISVTDTGIGMSEETRARLFSPFFTTKSDGTGLGL
ISCKRIAESYGGLIQVCSELGVGTRFDLILPARAPASRVSEENVPVVLGHGQRILIVDGEATRLSLLGNALASQGYQPQL
APDGGAALKLLRHHAEPDLVIIDSDILLLSAVCLLPTMQELGYRGPAIVLEDAGQPLRREHFSKDLAVHMLRKPLEMGRV
FRAVAYALT
>Mature_489_residues
MLKILLIGDSLEDAKLLFNTLREAGLDGSFERVESESALREALYLFAPDIVLSDLSMSGFHGYQALRVVREVGNTPFIFV
SSKMGEDIAVEALQEGANDYIIKQNLVRLPSAVTRAVRESRAELERQHVESELMRAQRLESLAMLAAGFSHDLRNILQPL
LIVPDLLAGRTDDPQLHQLVSIVAECGRRGHEMAESILSFVRGSNKPRERVLLADLFQTVQLLLRSSVPDCIALRIEECD
EYLSVEANYTELQQCLLNLALNAIHAMPTGGCLTLVAQRHDGDRICISVTDTGIGMSEETRARLFSPFFTTKSDGTGLGL
ISCKRIAESYGGLIQVCSELGVGTRFDLILPARAPASRVSEENVPVVLGHGQRILIVDGEATRLSLLGNALASQGYQPQL
APDGGAALKLLRHHAEPDLVIIDSDILLLSAVCLLPTMQELGYRGPAIVLEDAGQPLRREHFSKDLAVHMLRKPLEMGRV
FRAVAYALT

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=328, Percent_Identity=31.4024390243902, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788549, Length=227, Percent_Identity=31.2775330396476, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=29.8387096774194, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI48994928, Length=208, Percent_Identity=29.3269230769231, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788713, Length=251, Percent_Identity=28.6852589641434, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790300, Length=245, Percent_Identity=27.7551020408163, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081816, Length=342, Percent_Identity=25.7309941520468, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53605; Mature: 53605

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKILLIGDSLEDAKLLFNTLREAGLDGSFERVESESALREALYLFAPDIVLSDLSMSGF
CEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
HGYQALRVVREVGNTPFIFVSSKMGEDIAVEALQEGANDYIIKQNLVRLPSAVTRAVRES
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHH
RAELERQHVESELMRAQRLESLAMLAAGFSHDLRNILQPLLIVPDLLAGRTDDPQLHQLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SIVAECGRRGHEMAESILSFVRGSNKPRERVLLADLFQTVQLLLRSSVPDCIALRIEECD
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EYLSVEANYTELQQCLLNLALNAIHAMPTGGCLTLVAQRHDGDRICISVTDTGIGMSEET
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHH
RARLFSPFFTTKSDGTGLGLISCKRIAESYGGLIQVCSELGVGTRFDLILPARAPASRVS
HHHHHCCHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEEEECCCCHHHCC
EENVPVVLGHGQRILIVDGEATRLSLLGNALASQGYQPQLAPDGGAALKLLRHHAEPDLV
CCCCCEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEE
IIDSDILLLSAVCLLPTMQELGYRGPAIVLEDAGQPLRREHFSKDLAVHMLRKPLEMGRV
EECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FRAVAYALT
HHHHHHHCC
>Mature Secondary Structure
MLKILLIGDSLEDAKLLFNTLREAGLDGSFERVESESALREALYLFAPDIVLSDLSMSGF
CEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCC
HGYQALRVVREVGNTPFIFVSSKMGEDIAVEALQEGANDYIIKQNLVRLPSAVTRAVRES
HHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHH
RAELERQHVESELMRAQRLESLAMLAAGFSHDLRNILQPLLIVPDLLAGRTDDPQLHQLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SIVAECGRRGHEMAESILSFVRGSNKPRERVLLADLFQTVQLLLRSSVPDCIALRIEECD
HHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EYLSVEANYTELQQCLLNLALNAIHAMPTGGCLTLVAQRHDGDRICISVTDTGIGMSEET
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEEECCCCCCCHHH
RARLFSPFFTTKSDGTGLGLISCKRIAESYGGLIQVCSELGVGTRFDLILPARAPASRVS
HHHHHCCHHCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCEEEEEEECCCCHHHCC
EENVPVVLGHGQRILIVDGEATRLSLLGNALASQGYQPQLAPDGGAALKLLRHHAEPDLV
CCCCCEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCEE
IIDSDILLLSAVCLLPTMQELGYRGPAIVLEDAGQPLRREHFSKDLAVHMLRKPLEMGRV
EECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FRAVAYALT
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]