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The map label for this gene is dsbA [H]

Identifier: 182681248

GI number: 182681248

Start: 818794

End: 819576

Strand: Direct

Name: dsbA [H]

Synonym: XfasM23_0693

Alternate gene names: 182681248

Gene position: 818794-819576 (Clockwise)

Preceding gene: 182681247

Following gene: 182681249

Centisome position: 32.29

GC content: 51.98

Gene sequence:

>783_bases
ATGAAAAATCGCTTCGCTATGATGTTGATGGCGTTGTTGCCCGTTTTGGTGGCTTGCCAAGCTAAGGATGGTAGTGCGGA
TACCGTGCAGTCTGCCGTTCCAGCGCCGAGCGCACCGCCTAGCTCCTCCACGGCAACAACCACTCCGACCACACCGCCGC
CCGAGTCGGCGCGGCCGGTTGAAGCGTCGCGTGTGCCATCTGGGCCAAAACCAGTGGCTGGTACGGATTATGTTGTGATC
CAAGATGGCCAACAGTTTCAGCCTGTGTCTGGCAAGGTTGAGGTTGTTGAGGTGTTCGGTTACATCTGCCCGGCATGTTT
CCAGTTCCAACCACAGATTGCTCTATGGAAGGCAGGTCTAGCTTCGGATGTGAATTTTGTTTATGTCCCGGCAGTGTTTG
GTGGTCCTTGGGATGATTATGCGCGTGCGTTTTACGCTGCTGAGACTCTGAATTTGCAGGAGAAGACCCATCAGCAGTTG
TATAAAGCGATTCATGTTGATAGGACATTGAAAGGGGAGCGCGGTCGTGACACGGTGCAGGACATCGCTAATTTCTACGC
TAAGTTTGGCGTCAATCCGGAGCAGTTTGTGAATACGATGTCCAGCTTCGGTATCTCGGCAAAAGTGAATCGTGCTAAGC
AATTCGCTAAGCATAGTCAGGTCACGGGGACTCCTTCGTTGATTATCAACGGAAAATACTTGGTTAAAGGCCGTACTTAC
GATGACATGCTCCGGATTGCTGATCACCTGATTGAAGGTGAGCGTAAGGTGCTTGGCAAGTAG

Upstream 100 bases:

>100_bases
GGCCGGTGAGCCATAGATGAGGCAGGGTATGTGGCTCGTGTCATGATCACCGACATGCTGACTGTGGTCAGCTTCACTTT
CACTTTCGGAGAGAAAGCGT

Downstream 100 bases:

>100_bases
GCTTCGTTTGGTTACGTGAACGATTCTGATCAATGCATATTGCGGGTGTTGACTGCCAACATTCAGGCCGCTTCGAGTAC
TCGCCGCTATAGCGACTACG

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKNRFAMMLMALLPVLVACQAKDGSADTVQSAVPAPSAPPSSSTATTTPTTPPPESARPVEASRVPSGPKPVAGTDYVVI
QDGQQFQPVSGKVEVVEVFGYICPACFQFQPQIALWKAGLASDVNFVYVPAVFGGPWDDYARAFYAAETLNLQEKTHQQL
YKAIHVDRTLKGERGRDTVQDIANFYAKFGVNPEQFVNTMSSFGISAKVNRAKQFAKHSQVTGTPSLIINGKYLVKGRTY
DDMLRIADHLIEGERKVLGK

Sequences:

>Translated_260_residues
MKNRFAMMLMALLPVLVACQAKDGSADTVQSAVPAPSAPPSSSTATTTPTTPPPESARPVEASRVPSGPKPVAGTDYVVI
QDGQQFQPVSGKVEVVEVFGYICPACFQFQPQIALWKAGLASDVNFVYVPAVFGGPWDDYARAFYAAETLNLQEKTHQQL
YKAIHVDRTLKGERGRDTVQDIANFYAKFGVNPEQFVNTMSSFGISAKVNRAKQFAKHSQVTGTPSLIINGKYLVKGRTY
DDMLRIADHLIEGERKVLGK
>Mature_260_residues
MKNRFAMMLMALLPVLVACQAKDGSADTVQSAVPAPSAPPSSSTATTTPTTPPPESARPVEASRVPSGPKPVAGTDYVVI
QDGQQFQPVSGKVEVVEVFGYICPACFQFQPQIALWKAGLASDVNFVYVPAVFGGPWDDYARAFYAAETLNLQEKTHQQL
YKAIHVDRTLKGERGRDTVQDIANFYAKFGVNPEQFVNTMSSFGISAKVNRAKQFAKHSQVTGTPSLIINGKYLVKGRTY
DDMLRIADHLIEGERKVLGK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=162, Percent_Identity=25.3086419753086, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 28314; Mature: 28314

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRFAMMLMALLPVLVACQAKDGSADTVQSAVPAPSAPPSSSTATTTPTTPPPESARPV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EASRVPSGPKPVAGTDYVVIQDGQQFQPVSGKVEVVEVFGYICPACFQFQPQIALWKAGL
CHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCC
ASDVNFVYVPAVFGGPWDDYARAFYAAETLNLQEKTHQQLYKAIHVDRTLKGERGRDTVQ
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DIANFYAKFGVNPEQFVNTMSSFGISAKVNRAKQFAKHSQVTGTPSLIINGKYLVKGRTY
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCH
DDMLRIADHLIEGERKVLGK
HHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MKNRFAMMLMALLPVLVACQAKDGSADTVQSAVPAPSAPPSSSTATTTPTTPPPESARPV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
EASRVPSGPKPVAGTDYVVIQDGQQFQPVSGKVEVVEVFGYICPACFQFQPQIALWKAGL
CHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHCCC
ASDVNFVYVPAVFGGPWDDYARAFYAAETLNLQEKTHQQLYKAIHVDRTLKGERGRDTVQ
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DIANFYAKFGVNPEQFVNTMSSFGISAKVNRAKQFAKHSQVTGTPSLIINGKYLVKGRTY
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCEEEEECCCH
DDMLRIADHLIEGERKVLGK
HHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]