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The map label for this gene is dsbA [H]

Identifier: 182681247

GI number: 182681247

Start: 818055

End: 818702

Strand: Direct

Name: dsbA [H]

Synonym: XfasM23_0692

Alternate gene names: 182681247

Gene position: 818055-818702 (Clockwise)

Preceding gene: 182681245

Following gene: 182681248

Centisome position: 32.26

GC content: 53.4

Gene sequence:

>648_bases
ATGTCTCTGTTTGGACGTTTATTGTCTTTATTGCTTGCTTTTGTGCCTCTGAGTATTTGTGCTGCTGTGAATCACCTTCC
GGTTGTTGGTGAGGATTATGTCGAGATTCCCGATGGTCGGCCGTTTGCGCCGCTGGCTGGAAAGATCGAGGTGGTCGAAA
TTTTCGGTTATACCTGTCCGCATTGTGCACATTTTGATTCCAAGTTGCAGGCATGGGGTGCGCGTCAGGCGAAGGATGTG
CGTTTTACTTTAGTGCCTGCCGTGTTCGGCGGTGTTTGGGATCCTTTCGCGCGTGCTTATTTAGCTGCTGACGTTCTGGG
TGTCGCGAAGCGTAGCCATGCGGCAATGTTCGAGGCCATCCATGAGAAGGGCAGTGTGCCAATTCAGAACGTGGGGCCAG
ATGAACTGGCTGTCTTCTACGCCGGATATGGGGTGCAGCCGGATCGTTTTGTCGCTACTTTCAATGGCCCGGAAGTTGAG
AAGCGTTTCCAGGCTGCGCGTGCCTATGCGTTGAAGGTGCGTCCTGTCGGGACGCCGGCGATTGTAGTGGATGGCCGTTA
CATGGTAACTGGTCACGATTTTGACGATACGTTGCGCATTACCGATTATCTGGTGAGTCGTGAACGTGCTGCCTCACATG
GCCGGTGA

Upstream 100 bases:

>100_bases
CTGCTGTTATTATTACGCGTTTGTTTCTATAGCTATCTATAAGCTATAGCCAAGCATTTAATTTCTTTAGTTCCATAAGG
GCTTGTATCCCGGAGTGTCT

Downstream 100 bases:

>100_bases
GCCATAGATGAGGCAGGGTATGTGGCTCGTGTCATGATCACCGACATGCTGACTGTGGTCAGCTTCACTTTCACTTTCGG
AGAGAAAGCGTATGAAAAAT

Product: DSBA oxidoreductase

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSLFGRLLSLLLAFVPLSICAAVNHLPVVGEDYVEIPDGRPFAPLAGKIEVVEIFGYTCPHCAHFDSKLQAWGARQAKDV
RFTLVPAVFGGVWDPFARAYLAADVLGVAKRSHAAMFEAIHEKGSVPIQNVGPDELAVFYAGYGVQPDRFVATFNGPEVE
KRFQAARAYALKVRPVGTPAIVVDGRYMVTGHDFDDTLRITDYLVSRERAASHGR

Sequences:

>Translated_215_residues
MSLFGRLLSLLLAFVPLSICAAVNHLPVVGEDYVEIPDGRPFAPLAGKIEVVEIFGYTCPHCAHFDSKLQAWGARQAKDV
RFTLVPAVFGGVWDPFARAYLAADVLGVAKRSHAAMFEAIHEKGSVPIQNVGPDELAVFYAGYGVQPDRFVATFNGPEVE
KRFQAARAYALKVRPVGTPAIVVDGRYMVTGHDFDDTLRITDYLVSRERAASHGR
>Mature_214_residues
SLFGRLLSLLLAFVPLSICAAVNHLPVVGEDYVEIPDGRPFAPLAGKIEVVEIFGYTCPHCAHFDSKLQAWGARQAKDVR
FTLVPAVFGGVWDPFARAYLAADVLGVAKRSHAAMFEAIHEKGSVPIQNVGPDELAVFYAGYGVQPDRFVATFNGPEVEK
RFQAARAYALKVRPVGTPAIVVDGRYMVTGHDFDDTLRITDYLVSRERAASHGR

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 23518; Mature: 23387

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFGRLLSLLLAFVPLSICAAVNHLPVVGEDYVEIPDGRPFAPLAGKIEVVEIFGYTCP
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCEEEEEEECCCCC
HCAHFDSKLQAWGARQAKDVRFTLVPAVFGGVWDPFARAYLAADVLGVAKRSHAAMFEAI
CHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEKGSVPIQNVGPDELAVFYAGYGVQPDRFVATFNGPEVEKRFQAARAYALKVRPVGTPA
HHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHEEEEEEECCCCE
IVVDGRYMVTGHDFDDTLRITDYLVSRERAASHGR
EEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SLFGRLLSLLLAFVPLSICAAVNHLPVVGEDYVEIPDGRPFAPLAGKIEVVEIFGYTCP
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCEEEEEEECCCCC
HCAHFDSKLQAWGARQAKDVRFTLVPAVFGGVWDPFARAYLAADVLGVAKRSHAAMFEAI
CHHHHHHHHHHHCCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEKGSVPIQNVGPDELAVFYAGYGVQPDRFVATFNGPEVEKRFQAARAYALKVRPVGTPA
HHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHEEEEEEECCCCE
IVVDGRYMVTGHDFDDTLRITDYLVSRERAASHGR
EEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9099867 [H]