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The map label for this gene is truA

Identifier: 182681198

GI number: 182681198

Start: 758782

End: 759555

Strand: Direct

Name: truA

Synonym: XfasM23_0642

Alternate gene names: 182681198

Gene position: 758782-759555 (Clockwise)

Preceding gene: 182681197

Following gene: 182681199

Centisome position: 29.92

GC content: 56.72

Gene sequence:

>774_bases
ATGATGCGTTATGCGTTAGGCGTAGAGTACGACGGCAGCGAGTTTCTGGGCTGGCAACAATTGGGTGAGATGGGTCCTAG
CGTACAGGCGACACTACAGCAGGCTTTGGCTTCGGTAGCGGATTCGTCGGTGCGGGTGGTGTGTGCCGGTCGTACGGATG
CTGGGGTCCATGGCCAGTGCCAGGTTGTGCATTTTGATAGTGCGGTTACCCGTCCTCCACGTGCCTGGATATTGGGCACG
ACGACACGGTTACCATCCTCGGTGGCGGTACGTTGGTGTGTGCCGACATCCGAGGATTTTCATGCTCGCTTCTCCGCGTG
CGCACGCCGTTATCGTTATCGGTTGTTAAACCGTCAGGTGAGACCTGCGTTGCAGCATCAATTTTTGAGCTGGGAGCGGC
ATCCGTTGGATGCCCAGGCGATGCATGTTGCTGCGCAGATGTTGCTTGGAGAAAACGATTTCAGTGCGTTTCGCAGCGCG
CAGTGTCAGGCGCTACATGCGCGCCGTGAGCTTCAAGCGATTTCGGTACGGCGCGATGCCGAGGTCATCGAAATATGTGT
CCAAGCCAATGCCTTTCTTCATCACATGGTGCGCAATATCGTGGGATCGTTGCTTATGGTGGGTACCGGTGAACGTCCGA
TGGAATGGATTGCAGAGCTGCTTGCGGGGCGTGACCGGACCATGGCTGGACCGACTGCCTCAGCGCGTGGGCTGGTCTTT
GTCGGACCTTTGTATCCGGAAAAGTGGCACTTGCCTATGGAAGTGAGTGTATGA

Upstream 100 bases:

>100_bases
TCACCGGAGATGATGTTGCACGTGCCGAAGCAGCAGCATTACTTAATCAAATTGCATAACAGGCTCTGCCTGTCGGTTAA
GCTCCAGTGACCAGGATATG

Downstream 100 bases:

>100_bases
ACATCCCCCCTTACCGTACCCGTATCAAGTTTTGTGGTATGACCCGCGTTGGAGATGTGAGGTTAGCCAGCGAGCTTGGT
GTCGATGCGGTGGGACTCAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MMRYALGVEYDGSEFLGWQQLGEMGPSVQATLQQALASVADSSVRVVCAGRTDAGVHGQCQVVHFDSAVTRPPRAWILGT
TTRLPSSVAVRWCVPTSEDFHARFSACARRYRYRLLNRQVRPALQHQFLSWERHPLDAQAMHVAAQMLLGENDFSAFRSA
QCQALHARRELQAISVRRDAEVIEICVQANAFLHHMVRNIVGSLLMVGTGERPMEWIAELLAGRDRTMAGPTASARGLVF
VGPLYPEKWHLPMEVSV

Sequences:

>Translated_257_residues
MMRYALGVEYDGSEFLGWQQLGEMGPSVQATLQQALASVADSSVRVVCAGRTDAGVHGQCQVVHFDSAVTRPPRAWILGT
TTRLPSSVAVRWCVPTSEDFHARFSACARRYRYRLLNRQVRPALQHQFLSWERHPLDAQAMHVAAQMLLGENDFSAFRSA
QCQALHARRELQAISVRRDAEVIEICVQANAFLHHMVRNIVGSLLMVGTGERPMEWIAELLAGRDRTMAGPTASARGLVF
VGPLYPEKWHLPMEVSV
>Mature_257_residues
MMRYALGVEYDGSEFLGWQQLGEMGPSVQATLQQALASVADSSVRVVCAGRTDAGVHGQCQVVHFDSAVTRPPRAWILGT
TTRLPSSVAVRWCVPTSEDFHARFSACARRYRYRLLNRQVRPALQHQFLSWERHPLDAQAMHVAAQMLLGENDFSAFRSA
QCQALHARRELQAISVRRDAEVIEICVQANAFLHHMVRNIVGSLLMVGTGERPMEWIAELLAGRDRTMAGPTASARGLVF
VGPLYPEKWHLPMEVSV

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=277, Percent_Identity=32.129963898917, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1788657, Length=252, Percent_Identity=51.5873015873016, Blast_Score=268, Evalue=4e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_XYLF2 (B2I9J0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829358.1
- ProteinModelPortal:   B2I9J0
- SMR:   B2I9J0
- GeneID:   6202440
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0642
- HOGENOM:   HBG621020
- OMA:   NNYKLTI
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28664; Mature: 28664

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRYALGVEYDGSEFLGWQQLGEMGPSVQATLQQALASVADSSVRVVCAGRTDAGVHGQC
CCEEEEEEEECCCHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
QVVHFDSAVTRPPRAWILGTTTRLPSSVAVRWCVPTSEDFHARFSACARRYRYRLLNRQV
EEEEECCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
RPALQHQFLSWERHPLDAQAMHVAAQMLLGENDFSAFRSAQCQALHARRELQAISVRRDA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIEICVQANAFLHHMVRNIVGSLLMVGTGERPMEWIAELLAGRDRTMAGPTASARGLVF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VGPLYPEKWHLPMEVSV
EECCCCCCCCCCEEECC
>Mature Secondary Structure
MMRYALGVEYDGSEFLGWQQLGEMGPSVQATLQQALASVADSSVRVVCAGRTDAGVHGQC
CCEEEEEEEECCCHHCCHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
QVVHFDSAVTRPPRAWILGTTTRLPSSVAVRWCVPTSEDFHARFSACARRYRYRLLNRQV
EEEEECCCCCCCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
RPALQHQFLSWERHPLDAQAMHVAAQMLLGENDFSAFRSAQCQALHARRELQAISVRRDA
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVIEICVQANAFLHHMVRNIVGSLLMVGTGERPMEWIAELLAGRDRTMAGPTASARGLVF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
VGPLYPEKWHLPMEVSV
EECCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA