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The map label for this gene is relA [H]

Identifier: 182681151

GI number: 182681151

Start: 701947

End: 704103

Strand: Direct

Name: relA [H]

Synonym: XfasM23_0593

Alternate gene names: 182681151

Gene position: 701947-704103 (Clockwise)

Preceding gene: 182681150

Following gene: 182681152

Centisome position: 27.68

GC content: 53.87

Gene sequence:

>2157_bases
GTGAATGTCTCATTATTTCCTCAAGAACTGGATGTCTTACTGCGACGCTCCGGGGTATCTACACTGAATCCGGAATTGTC
TCAGGGGTTAGTGCAAGCGTGCCAGGCATTGCCTTCTGAAGCTGGGCAGCAGGTGGCCTGGCCGGTGTTGCTAGATATGT
TGGATGCACTGGTGGTGTTGTCGGCGGATGAAACATTGCTGATTGCAGCGTTGTTGTTTGATCTGCCAATGCTGCGTCCA
ATGTTGCCCGTGTTGCCGTGGCGTTCGAGTACGCATCAGCAAATGGTCGGGAGTTTGCTTGATGGCCAGGATGCAGCGGA
ACAAGTGTGGACGTTGCATGCTGGATGTGCGGCTGGACGTAATGGTGAGGGGTTGCGTCGGCTGTTGCTGGCGCTTGTGC
ATGATCTGCGTGTGGTGCCGATTTTACTTGCCCGACAGTTGGCCAAGATGCGTGCTGCAGGTGTGTTGCTTGAGTCGCAG
CGGCGTGCGTTGGCGCAATTGACCCGTGATATCCATGCGCCGTTGGCTAACCGGCTTGGTATTTGGCAGTTGAAGTGGGA
ATTAGAAGATCTGGCGTTCTGTTATTTGGAGTCAGAGATCTATCGGAGTATCGCGTCTGCCTTGGATGAATCCCGTGTTG
CGCGTGAGCGTTATATCAGTGTGATTGCTATGGAGTTATCGCGGGTGCTTGCTGAGCATGGTGTGTGTGCTGAGGTCAGT
GGGCGTCCGAAACACATTTACAGTATTTGGAGAAAGATGCAGAAGAAGCGGTTGCATTTCGAACAGGTGTACGACTTGCA
GGCGGTTCGTGTGATGGTTGAGGACATGGCCGCTTGTTATGCCGCCCTTGGAGTGGTGCATGCGTTGTGGGCACCGGTGC
CTGGTGAGTTTGATGATTACATCGCGCGGCCCAAGGCGAATGACTACCGCTCACTACACACGGCAGTGTTGGGACCGGAA
GAGCGTACTGTAGAAGTACAAATCCGCACGCACGAAATGCACGCGCACGCTGAATTGGGTGTGGCTGCGCACTGGAAATA
CAAAGAGGATGGCAAAGGTGCGGAGAGGGATTTTGATCGCAAGATCGTCTGGATGCGGCAGTTATTGGAGCAAGCTCAAG
AGGGTGAGTTCGGCGAGGTGGTGGAGGCACTGGATACGGAGTTGCTAGAGGATCGGGTTTACGCGTTAACTCCTAAAGGC
GAGGTGATTGATGTGCCGCAGGGGGCGACGCCACTAGATTTTGCTTATCACGTGCATACCATGGTCGGGCATTGTTGCCG
GGGGGCAAAAGTGAATGGCCGCATTGTGCCATTGACTTACCGTTTGCGAAGTGGTGATCGTGTTGAGATTTTGACGGCGA
AGGAAGCCAATCCGCATCGTGATTGGTTACTTCGGGCGAATGGTTTCCTCGCCAGTCGCCGTTCTCGTGACAAGGTGAGG
GCTTGGTTCAATAAGATCGAACGGGCGCGTAACGTGCAGGTGGGAAAGGAGTCGCTGGACCGTGAGCTAAAACGGGTGGG
ACAGCAGTACGTGGATCTTAGTCCTATCGCGCGGCGTTTCCATGCCGATAGTGTGGACGATTTGTACGTCCAGATTGCAC
TTGGCAATATTGGGCCGAATCAAGTAAGCCGTGCTTTGCTGGAGACTGAGCGTGTTGTTGCATTTGCGCCGCTCGCGCGG
CCAAGCAAGAAGCGTCACGGTCTAAGCAAATCAATGTTCATTGTGCAGGGGGTCAGCAATATATTGGTGCAGCTGGCGCG
TTGTTGTCAGCCGGTGTGTGGTGAGCCGATTGCCGGTTACTTGACCCTAAGCCGTGGGGTGACGGTACATCGTGCTGACT
GTGCTGTATTGATGCGCTTGAATGCCATGCATCCGCAGCGGGTGTTATCGGTTGAATGGGGCAGTCAACTCAGCGGTAGT
TATGAGGTCGAGATTTTGGTGCGTGCGGTAAACCGGCGTTGGTTGTTGAAGGACATCGCCGATGTGATTGCACAAGAAGA
TGCGCATATGTTGGAAATGAGTAGTGATGCGTTTCGAGACAATGGTTATCTTCAATTTCGGGTACGGCTCAGGCTGAGTC
ATCACCGGCAATTGGCGGCGTTGTTGGGTAAATTGGATGGCCTTTCCGGTGTCAATGAAGTGCGGAGGATGGGCTGA

Upstream 100 bases:

>100_bases
CGCAGTAGGTTTAACCCAATGCTGACTATTCGTTTATGCGATCTTAAAAAGCCTGTGTTTCAAGGTTGGGATGGCGCACA
CATTGGATGGGTAGTTTGTA

Downstream 100 bases:

>100_bases
TCGTAGCGTATATGTCCGCTACAGTAGGGAATGTATGTCAGTGTTGTTACGAGGTTTATGCTTGGAGTGTTGAGTGTCAT
TGTTCACTGTCAGGATGTTT

Product: RelA/SpoT family protein

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase; ppGpp synthase I [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MNVSLFPQELDVLLRRSGVSTLNPELSQGLVQACQALPSEAGQQVAWPVLLDMLDALVVLSADETLLIAALLFDLPMLRP
MLPVLPWRSSTHQQMVGSLLDGQDAAEQVWTLHAGCAAGRNGEGLRRLLLALVHDLRVVPILLARQLAKMRAAGVLLESQ
RRALAQLTRDIHAPLANRLGIWQLKWELEDLAFCYLESEIYRSIASALDESRVARERYISVIAMELSRVLAEHGVCAEVS
GRPKHIYSIWRKMQKKRLHFEQVYDLQAVRVMVEDMAACYAALGVVHALWAPVPGEFDDYIARPKANDYRSLHTAVLGPE
ERTVEVQIRTHEMHAHAELGVAAHWKYKEDGKGAERDFDRKIVWMRQLLEQAQEGEFGEVVEALDTELLEDRVYALTPKG
EVIDVPQGATPLDFAYHVHTMVGHCCRGAKVNGRIVPLTYRLRSGDRVEILTAKEANPHRDWLLRANGFLASRRSRDKVR
AWFNKIERARNVQVGKESLDRELKRVGQQYVDLSPIARRFHADSVDDLYVQIALGNIGPNQVSRALLETERVVAFAPLAR
PSKKRHGLSKSMFIVQGVSNILVQLARCCQPVCGEPIAGYLTLSRGVTVHRADCAVLMRLNAMHPQRVLSVEWGSQLSGS
YEVEILVRAVNRRWLLKDIADVIAQEDAHMLEMSSDAFRDNGYLQFRVRLRLSHHRQLAALLGKLDGLSGVNEVRRMG

Sequences:

>Translated_718_residues
MNVSLFPQELDVLLRRSGVSTLNPELSQGLVQACQALPSEAGQQVAWPVLLDMLDALVVLSADETLLIAALLFDLPMLRP
MLPVLPWRSSTHQQMVGSLLDGQDAAEQVWTLHAGCAAGRNGEGLRRLLLALVHDLRVVPILLARQLAKMRAAGVLLESQ
RRALAQLTRDIHAPLANRLGIWQLKWELEDLAFCYLESEIYRSIASALDESRVARERYISVIAMELSRVLAEHGVCAEVS
GRPKHIYSIWRKMQKKRLHFEQVYDLQAVRVMVEDMAACYAALGVVHALWAPVPGEFDDYIARPKANDYRSLHTAVLGPE
ERTVEVQIRTHEMHAHAELGVAAHWKYKEDGKGAERDFDRKIVWMRQLLEQAQEGEFGEVVEALDTELLEDRVYALTPKG
EVIDVPQGATPLDFAYHVHTMVGHCCRGAKVNGRIVPLTYRLRSGDRVEILTAKEANPHRDWLLRANGFLASRRSRDKVR
AWFNKIERARNVQVGKESLDRELKRVGQQYVDLSPIARRFHADSVDDLYVQIALGNIGPNQVSRALLETERVVAFAPLAR
PSKKRHGLSKSMFIVQGVSNILVQLARCCQPVCGEPIAGYLTLSRGVTVHRADCAVLMRLNAMHPQRVLSVEWGSQLSGS
YEVEILVRAVNRRWLLKDIADVIAQEDAHMLEMSSDAFRDNGYLQFRVRLRLSHHRQLAALLGKLDGLSGVNEVRRMG
>Mature_718_residues
MNVSLFPQELDVLLRRSGVSTLNPELSQGLVQACQALPSEAGQQVAWPVLLDMLDALVVLSADETLLIAALLFDLPMLRP
MLPVLPWRSSTHQQMVGSLLDGQDAAEQVWTLHAGCAAGRNGEGLRRLLLALVHDLRVVPILLARQLAKMRAAGVLLESQ
RRALAQLTRDIHAPLANRLGIWQLKWELEDLAFCYLESEIYRSIASALDESRVARERYISVIAMELSRVLAEHGVCAEVS
GRPKHIYSIWRKMQKKRLHFEQVYDLQAVRVMVEDMAACYAALGVVHALWAPVPGEFDDYIARPKANDYRSLHTAVLGPE
ERTVEVQIRTHEMHAHAELGVAAHWKYKEDGKGAERDFDRKIVWMRQLLEQAQEGEFGEVVEALDTELLEDRVYALTPKG
EVIDVPQGATPLDFAYHVHTMVGHCCRGAKVNGRIVPLTYRLRSGDRVEILTAKEANPHRDWLLRANGFLASRRSRDKVR
AWFNKIERARNVQVGKESLDRELKRVGQQYVDLSPIARRFHADSVDDLYVQIALGNIGPNQVSRALLETERVVAFAPLAR
PSKKRHGLSKSMFIVQGVSNILVQLARCCQPVCGEPIAGYLTLSRGVTVHRADCAVLMRLNAMHPQRVLSVEWGSQLSGS
YEVEILVRAVNRRWLLKDIADVIAQEDAHMLEMSSDAFRDNGYLQFRVRLRLSHHRQLAALLGKLDGLSGVNEVRRMG

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the formation of pppGpp which i

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=41.1420204978038, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1790082, Length=674, Percent_Identity=33.9762611275964, Blast_Score=373, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 80759; Mature: 80759

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSLFPQELDVLLRRSGVSTLNPELSQGLVQACQALPSEAGQQVAWPVLLDMLDALVVL
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHH
SADETLLIAALLFDLPMLRPMLPVLPWRSSTHQQMVGSLLDGQDAAEQVWTLHAGCAAGR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
NGEGLRRLLLALVHDLRVVPILLARQLAKMRAAGVLLESQRRALAQLTRDIHAPLANRLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IWQLKWELEDLAFCYLESEIYRSIASALDESRVARERYISVIAMELSRVLAEHGVCAEVS
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GRPKHIYSIWRKMQKKRLHFEQVYDLQAVRVMVEDMAACYAALGVVHALWAPVPGEFDDY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IARPKANDYRSLHTAVLGPEERTVEVQIRTHEMHAHAELGVAAHWKYKEDGKGAERDFDR
HCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHCCEEEEECCCCCCCCCCHHHHH
KIVWMRQLLEQAQEGEFGEVVEALDTELLEDRVYALTPKGEVIDVPQGATPLDFAYHVHT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHH
MVGHCCRGAKVNGRIVPLTYRLRSGDRVEILTAKEANPHRDWLLRANGFLASRRSRDKVR
HHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCHHHEEECCCCHHHCCCHHHHH
AWFNKIERARNVQVGKESLDRELKRVGQQYVDLSPIARRFHADSVDDLYVQIALGNIGPN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCHH
QVSRALLETERVVAFAPLARPSKKRHGLSKSMFIVQGVSNILVQLARCCQPVCGEPIAGY
HHHHHHHHHHHHHEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCC
LTLSRGVTVHRADCAVLMRLNAMHPQRVLSVEWGSQLSGSYEVEILVRAVNRRWLLKDIA
EEECCCCEEEHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DVIAQEDAHMLEMSSDAFRDNGYLQFRVRLRLSHHRQLAALLGKLDGLSGVNEVRRMG
HHHHCCCHHHHHCCCHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCC
>Mature Secondary Structure
MNVSLFPQELDVLLRRSGVSTLNPELSQGLVQACQALPSEAGQQVAWPVLLDMLDALVVL
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHH
SADETLLIAALLFDLPMLRPMLPVLPWRSSTHQQMVGSLLDGQDAAEQVWTLHAGCAAGR
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
NGEGLRRLLLALVHDLRVVPILLARQLAKMRAAGVLLESQRRALAQLTRDIHAPLANRLG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IWQLKWELEDLAFCYLESEIYRSIASALDESRVARERYISVIAMELSRVLAEHGVCAEVS
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECC
GRPKHIYSIWRKMQKKRLHFEQVYDLQAVRVMVEDMAACYAALGVVHALWAPVPGEFDDY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IARPKANDYRSLHTAVLGPEERTVEVQIRTHEMHAHAELGVAAHWKYKEDGKGAERDFDR
HCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEHHHHHHHCCEEEEECCCCCCCCCCHHHHH
KIVWMRQLLEQAQEGEFGEVVEALDTELLEDRVYALTPKGEVIDVPQGATPLDFAYHVHT
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHH
MVGHCCRGAKVNGRIVPLTYRLRSGDRVEILTAKEANPHRDWLLRANGFLASRRSRDKVR
HHHHHHCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCHHHEEECCCCHHHCCCHHHHH
AWFNKIERARNVQVGKESLDRELKRVGQQYVDLSPIARRFHADSVDDLYVQIALGNIGPN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCCHH
QVSRALLETERVVAFAPLARPSKKRHGLSKSMFIVQGVSNILVQLARCCQPVCGEPIAGY
HHHHHHHHHHHHHEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCC
LTLSRGVTVHRADCAVLMRLNAMHPQRVLSVEWGSQLSGSYEVEILVRAVNRRWLLKDIA
EEECCCCEEEHHHHHHHHHHHCCCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHH
DVIAQEDAHMLEMSSDAFRDNGYLQFRVRLRLSHHRQLAALLGKLDGLSGVNEVRRMG
HHHHCCCHHHHHCCCHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7928955 [H]