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The map label for this gene is queA

Identifier: 182681150

GI number: 182681150

Start: 700176

End: 701219

Strand: Direct

Name: queA

Synonym: XfasM23_0592

Alternate gene names: 182681150

Gene position: 700176-701219 (Clockwise)

Preceding gene: 182681149

Following gene: 182681151

Centisome position: 27.61

GC content: 51.15

Gene sequence:

>1044_bases
GTGTTGAAAAAGTCCGATTTTCATTACGATTTACCTGAGGAGCTGATTGCTCAAGGCCCTTTGCCTGAACGCTCAGCCAG
TCGCCTGATGCTTGTGCCGTCGGCCCCAGAGCAATTTCAGGATTGTTATGTTCGCGATTTGCCGGAATTGCTGCAGCCTG
GTGATTTGCTGGTGTTCAACGATACCCGAGTGATCCCCGCGCGATTGTTTGGTAGGAAGGTGAGCGGTGGTCGGGTTGAA
CTCCTGATTGAGCGTTTTCTTGGTACCCATCAGGCAGTGGTGCAGTTGCGTACCAGCAGGTCGCTCAAAGTGGGAAATAG
AATTTTGTTGGATGCGGGCGGTCATGCTGAGGTGCTTGGTCGTGATGGTGAGTTCTATTTATTGAGTTTCGATGTTGAGT
CACCTTTGGAACAATGGTTATCGGATGTCGGTCAGTTGCCATTGCCACCTTATATTCATCGTGAGCCTGATGAATATGAT
CGTGAACGTTATCAAACAGTGTTTGCACGCTCAGTGGGTGCAGTTGCGGCGCCTACTGCTGGTTTGCATTTTGACGAGTC
GTTGTTGGCACGGCTGCGTGCACGTGGGGTAGAGTTTGGCTATATCACCCTGCATGTAGGAGCGGGTACGTTTCAGCCAG
TGCGGGTAGCGTTGCTGCAAGAGCATGTGATGCACAGTGAATGGTTCAAGGTTGGCGCTGAATTAGTCGAGCAAGTGCGT
AGTGCCCGTGCGCGGGGCGGTCGAGTCATTGCTGTTGGTACGACTGTGGTGCGCTCATTGGAAAGTGCGATGCGTCATGG
GGAGCTGCAACCATTTGTTGGTGAGACTCAGATCTTTATTTTTCCCGGTTATTGCATCCGTAGCGTGGATGCAATGGTGA
CTAACTTTCATTTGCCTGAGAGTACGTTGTTGATGCTGGTTGCTGCTTTTGCGGGACGTACGCGGATTCTTGATGCTTAC
TATCATGCAGTGCAGCAGCGTTACCGTTTTTTCAGCTACGGTGACGCGATGTTATTGTTCCCGCGTAATGCTGGTGAGCA
ATAG

Upstream 100 bases:

>100_bases
GTTGAGCATTGTGATGAGGGAAAAAGTTAGCTATGTCTATCCAGCCTTTGGGTACGTTAGTCTCTCTTTATAATCCCAAG
CCTGCAGAACAAGTGGTTCT

Downstream 100 bases:

>100_bases
TCTTGCTTAGTCATTTCATTGCATTGATCGGAGATAACGTCATGCTGTAATCAGCTGCGATTGCTGCGTAAATCATGTGG
TGCATCCGGTTGGCGGCATC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLKKSDFHYDLPEELIAQGPLPERSASRLMLVPSAPEQFQDCYVRDLPELLQPGDLLVFNDTRVIPARLFGRKVSGGRVE
LLIERFLGTHQAVVQLRTSRSLKVGNRILLDAGGHAEVLGRDGEFYLLSFDVESPLEQWLSDVGQLPLPPYIHREPDEYD
RERYQTVFARSVGAVAAPTAGLHFDESLLARLRARGVEFGYITLHVGAGTFQPVRVALLQEHVMHSEWFKVGAELVEQVR
SARARGGRVIAVGTTVVRSLESAMRHGELQPFVGETQIFIFPGYCIRSVDAMVTNFHLPESTLLMLVAAFAGRTRILDAY
YHAVQQRYRFFSYGDAMLLFPRNAGEQ

Sequences:

>Translated_347_residues
MLKKSDFHYDLPEELIAQGPLPERSASRLMLVPSAPEQFQDCYVRDLPELLQPGDLLVFNDTRVIPARLFGRKVSGGRVE
LLIERFLGTHQAVVQLRTSRSLKVGNRILLDAGGHAEVLGRDGEFYLLSFDVESPLEQWLSDVGQLPLPPYIHREPDEYD
RERYQTVFARSVGAVAAPTAGLHFDESLLARLRARGVEFGYITLHVGAGTFQPVRVALLQEHVMHSEWFKVGAELVEQVR
SARARGGRVIAVGTTVVRSLESAMRHGELQPFVGETQIFIFPGYCIRSVDAMVTNFHLPESTLLMLVAAFAGRTRILDAY
YHAVQQRYRFFSYGDAMLLFPRNAGEQ
>Mature_347_residues
MLKKSDFHYDLPEELIAQGPLPERSASRLMLVPSAPEQFQDCYVRDLPELLQPGDLLVFNDTRVIPARLFGRKVSGGRVE
LLIERFLGTHQAVVQLRTSRSLKVGNRILLDAGGHAEVLGRDGEFYLLSFDVESPLEQWLSDVGQLPLPPYIHREPDEYD
RERYQTVFARSVGAVAAPTAGLHFDESLLARLRARGVEFGYITLHVGAGTFQPVRVALLQEHVMHSEWFKVGAELVEQVR
SARARGGRVIAVGTTVVRSLESAMRHGELQPFVGETQIFIFPGYCIRSVDAMVTNFHLPESTLLMLVAAFAGRTRILDAY
YHAVQQRYRFFSYGDAMLLFPRNAGEQ

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=343, Percent_Identity=52.7696793002915, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_XYLF2 (B2I961)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829310.1
- ProteinModelPortal:   B2I961
- SMR:   B2I961
- GeneID:   6202867
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0592
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39024; Mature: 39024

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKSDFHYDLPEELIAQGPLPERSASRLMLVPSAPEQFQDCYVRDLPELLQPGDLLVFN
CCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEC
DTRVIPARLFGRKVSGGRVELLIERFLGTHQAVVQLRTSRSLKVGNRILLDAGGHAEVLG
CCCEEHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCEECCCEEEEECCCCEEEEE
RDGEFYLLSFDVESPLEQWLSDVGQLPLPPYIHREPDEYDRERYQTVFARSVGAVAAPTA
CCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCC
GLHFDESLLARLRARGVEFGYITLHVGAGTFQPVRVALLQEHVMHSEWFKVGAELVEQVR
CCCCCHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARARGGRVIAVGTTVVRSLESAMRHGELQPFVGETQIFIFPGYCIRSVDAMVTNFHLPE
HHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCH
STLLMLVAAFAGRTRILDAYYHAVQQRYRFFSYGDAMLLFPRNAGEQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
>Mature Secondary Structure
MLKKSDFHYDLPEELIAQGPLPERSASRLMLVPSAPEQFQDCYVRDLPELLQPGDLLVFN
CCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEC
DTRVIPARLFGRKVSGGRVELLIERFLGTHQAVVQLRTSRSLKVGNRILLDAGGHAEVLG
CCCEEHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCEECCCEEEEECCCCEEEEE
RDGEFYLLSFDVESPLEQWLSDVGQLPLPPYIHREPDEYDRERYQTVFARSVGAVAAPTA
CCCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHCCCC
GLHFDESLLARLRARGVEFGYITLHVGAGTFQPVRVALLQEHVMHSEWFKVGAELVEQVR
CCCCCHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SARARGGRVIAVGTTVVRSLESAMRHGELQPFVGETQIFIFPGYCIRSVDAMVTNFHLPE
HHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCH
STLLMLVAAFAGRTRILDAYYHAVQQRYRFFSYGDAMLLFPRNAGEQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA