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The map label for this gene is lip2 [H]

Identifier: 182681100

GI number: 182681100

Start: 645147

End: 646160

Strand: Reverse

Name: lip2 [H]

Synonym: XfasM23_0542

Alternate gene names: 182681100

Gene position: 646160-645147 (Counterclockwise)

Preceding gene: 182681102

Following gene: 182681097

Centisome position: 25.48

GC content: 55.72

Gene sequence:

>1014_bases
ATGAAATTCACCCGCAAACTAGCCACCGCTGCCATCTTGGCACTGGCATCCACCTCATCCCAAGCCGCCGGCAGCCCTGG
TGTCGAACACCGCACCCAAGCCTTCCTCGAAGACTTAGCGAAGGGAGGGGGAGCACCGATCGAAACCCTGTCGCCTGCCA
ACGCACGCCAAGTGCTTGTAAACGCACAAAAGGGAGCCAAACTGCCACCTGCAGACGTGAGCGAAAAGACCATCACCATT
GATGGCAAGCAGATTGTGTTGAACATCGTTCGTCCAGCCGGCGTCAAAGGCATCCTGCCTGGCTTCATGTTCGTCCACGG
CGGCGGCTGGGTTCTTGGAGACTTCCAAACCCACGAACGCCTTGTACGTGATCTGGTGTCCGATTCCGGCGCAGTAGCCA
TCTTCGTAAACTACACCCCGTCCCCGGAAGCACGCTACCCAGTGGCTATCAATGAAATCTACGCAGCAACCCAGTGGGTA
GCCGAACACGGCGCGCAGATCAATGTCGACGGTCAACGCCTCGCCATCGTGGGAAACAGCGTCGGCGGCAATATGGCAAC
CGTGGTAGCACAGATGGCCAAAGAAAAAGGCACACCAGCGCTACGCGCACAAGTGCTGTTCTGGCCAGTCACTAATGCAA
ACTTTGAAAACACCTCTTACAACGAATTCGCAAACGGTCACTTCCTTACAAAAAACATGATGAAATGGTTCTGGGATGCC
TATACCACTGATTCCAAACAGCGTCAGGACATCTACGCATCACCGCTGCTAGCCACAGCCGAACAATTGAAGGGCCTACC
ACCCACCCTGATCCAAACCGCTGAGAAAGATGTGCTCCGTGACGAAGGCGAGGCCTATGCACGCAAACTTGACGCAGCCG
GCGTCAACGTCGTCATCACCCGCTACAACGGCATGATTCACGACTTCGGGCTCCTCAATGTACTCGCCGACCTACCTGCC
ACTCGCGCTGCGTTACACCAGGCAAGCGAAGAACTGAAGATACACCTGCGTTAA

Upstream 100 bases:

>100_bases
AAACTCGCAGTACCCGACCGTACTTCAACATCACTCACAACAACATCAACGTGCATTTGAATGTCCCAAATGTACCCACC
TATCACTGAGGATCAACCCT

Downstream 100 bases:

>100_bases
CGTCTGACGCTAGCTGGCCCAACATCCGATTGCTGTTGGGCTAGCCCCCTGAATGTTTTAAAGACGCCTCGAGAAAAATT
CCAAATCCCGCCCCACACAC

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKFTRKLATAAILALASTSSQAAGSPGVEHRTQAFLEDLAKGGGAPIETLSPANARQVLVNAQKGAKLPPADVSEKTITI
DGKQIVLNIVRPAGVKGILPGFMFVHGGGWVLGDFQTHERLVRDLVSDSGAVAIFVNYTPSPEARYPVAINEIYAATQWV
AEHGAQINVDGQRLAIVGNSVGGNMATVVAQMAKEKGTPALRAQVLFWPVTNANFENTSYNEFANGHFLTKNMMKWFWDA
YTTDSKQRQDIYASPLLATAEQLKGLPPTLIQTAEKDVLRDEGEAYARKLDAAGVNVVITRYNGMIHDFGLLNVLADLPA
TRAALHQASEELKIHLR

Sequences:

>Translated_337_residues
MKFTRKLATAAILALASTSSQAAGSPGVEHRTQAFLEDLAKGGGAPIETLSPANARQVLVNAQKGAKLPPADVSEKTITI
DGKQIVLNIVRPAGVKGILPGFMFVHGGGWVLGDFQTHERLVRDLVSDSGAVAIFVNYTPSPEARYPVAINEIYAATQWV
AEHGAQINVDGQRLAIVGNSVGGNMATVVAQMAKEKGTPALRAQVLFWPVTNANFENTSYNEFANGHFLTKNMMKWFWDA
YTTDSKQRQDIYASPLLATAEQLKGLPPTLIQTAEKDVLRDEGEAYARKLDAAGVNVVITRYNGMIHDFGLLNVLADLPA
TRAALHQASEELKIHLR
>Mature_337_residues
MKFTRKLATAAILALASTSSQAAGSPGVEHRTQAFLEDLAKGGGAPIETLSPANARQVLVNAQKGAKLPPADVSEKTITI
DGKQIVLNIVRPAGVKGILPGFMFVHGGGWVLGDFQTHERLVRDLVSDSGAVAIFVNYTPSPEARYPVAINEIYAATQWV
AEHGAQINVDGQRLAIVGNSVGGNMATVVAQMAKEKGTPALRAQVLFWPVTNANFENTSYNEFANGHFLTKNMMKWFWDA
YTTDSKQRQDIYASPLLATAEQLKGLPPTLIQTAEKDVLRDEGEAYARKLDAAGVNVVITRYNGMIHDFGLLNVLADLPA
TRAALHQASEELKIHLR

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=294, Percent_Identity=25.8503401360544, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI157041239, Length=255, Percent_Identity=25.0980392156863, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI21328446, Length=92, Percent_Identity=34.7826086956522, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI68051721, Length=191, Percent_Identity=25.130890052356, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=211, Percent_Identity=28.9099526066351, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=301, Percent_Identity=27.5747508305648, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71996133, Length=124, Percent_Identity=37.0967741935484, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=141, Percent_Identity=34.0425531914894, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=124, Percent_Identity=32.258064516129, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 36385; Mature: 36385

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTRKLATAAILALASTSSQAAGSPGVEHRTQAFLEDLAKGGGAPIETLSPANARQVLV
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEE
NAQKGAKLPPADVSEKTITIDGKQIVLNIVRPAGVKGILPGFMFVHGGGWVLGDFQTHER
ECCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCHHCCCEEEEECCCEEECCHHHHHH
LVRDLVSDSGAVAIFVNYTPSPEARYPVAINEIYAATQWVAEHGAQINVDGQRLAIVGNS
HHHHHHCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCEEEECCCEEEEEECC
VGGNMATVVAQMAKEKGTPALRAQVLFWPVTNANFENTSYNEFANGHFLTKNMMKWFWDA
CCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
YTTDSKQRQDIYASPLLATAEQLKGLPPTLIQTAEKDVLRDEGEAYARKLDAAGVNVVIT
HCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEE
RYNGMIHDFGLLNVLADLPATRAALHQASEELKIHLR
ECCCCCHHHHHHHHHHHCCHHHHHHHHCCHHEEEECC
>Mature Secondary Structure
MKFTRKLATAAILALASTSSQAAGSPGVEHRTQAFLEDLAKGGGAPIETLSPANARQVLV
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEE
NAQKGAKLPPADVSEKTITIDGKQIVLNIVRPAGVKGILPGFMFVHGGGWVLGDFQTHER
ECCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCHHCCCEEEEECCCEEECCHHHHHH
LVRDLVSDSGAVAIFVNYTPSPEARYPVAINEIYAATQWVAEHGAQINVDGQRLAIVGNS
HHHHHHCCCCCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHCCCEEEECCCEEEEEECC
VGGNMATVVAQMAKEKGTPALRAQVLFWPVTNANFENTSYNEFANGHFLTKNMMKWFWDA
CCCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHH
YTTDSKQRQDIYASPLLATAEQLKGLPPTLIQTAEKDVLRDEGEAYARKLDAAGVNVVIT
HCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEEE
RYNGMIHDFGLLNVLADLPATRAALHQASEELKIHLR
ECCCCCHHHHHHHHHHHCCHHHHHHHHCCHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]