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The map label for this gene is mii [H]

Identifier: 182681093

GI number: 182681093

Start: 633809

End: 635005

Strand: Direct

Name: mii [H]

Synonym: XfasM23_0535

Alternate gene names: 182681093

Gene position: 633809-635005 (Clockwise)

Preceding gene: 182681092

Following gene: 182681098

Centisome position: 25.0

GC content: 57.81

Gene sequence:

>1197_bases
ATGCTACCTCTCCCTCAATGCCGTATCCCTGCGACGTATCTACGTGGCGGTACCAGCAAAGGTGTGTTCTTTCGCTTGGA
GGATTTGCCTCCAGCCTGCCGTGTGCCAGGCCCTGCGCGTGATGCCTTGTTACTGCGTGTCATCGGCAGCCCAGATCCCT
ATGGTAAGCAGATCGACGGAATGGGAGGCGGCACCTCAAGTACCAGTAAAGTGGTGATCATCGCGCCGAGTGCCTATCCC
AATCACGATGTGGATTATTTGTTTGGTCAAGTTGCGATTGAGGGTGCCTGTGTCGATTGGACAGGAAATTGCGGCAATTT
GTCCGCCGCAGTGGGGCCATTTGCCATTGCTGCCGGTATTGTCGATCCGGCACGGGTGCCACACCGCGGGATATGCAGTG
TACGGATTTGGCAAGCCAATATCGGTAAAACCATCGTTGCACATGTGCCCATCCTTGATGGACAGGTGCAGGAGAGTGGC
GATTTCATGATGGAGGGGGCTGCTTTTCCGGGGGCTGAAGTGCGCTTGGAGTTTCTCAACCCTGCGGATGTGGGCGGAGA
TCGCGTGATGTTTCCGACGGGGCACCTGATCGATACGTTGGATGTGCCAGGATTGGGGCCGATTGAAGCCACCTTGATCA
ATGCAGGGATACCGATGCTTTGTGTGGATGCCCATGCCCTGGGCTACACCGGTACCGAGCTACAAGAGGCGATCAATGGC
GATCCGCATGCGTTGGTTTGTCTGGAGACGTTACGCGCCTATGGGGCGTTGCGTATGGGAGTGATCGGACATTTAGAAGA
CATGGTCCAGCATCAGCACACCCCTAAACTTGCTTTTGTTGCAGCTCCTGCCGATTACGTCGCCTCCGGTGGTCGGCACG
TACATGCTACAGAAATTGATGTCCTGGTGCGGGCAATGTCCATGGGCCGGTTGCACCACGCGATGATGGGTACCGCCGCC
GTGGCGCTGGGGACGGCGGCTGCAATCCCCGGCACGCTGGTCAATCGTGCGGCTGGTGGCGGGCTGCGTCGTGTGGTCCG
CTTTGGCCATCCCTCCGGCACCTTGCAAGTTGGGGCCGAAGTACATCAAGTGAATGGGCAATGGTCTGTCGCCAATGTGC
GGATGAGCCGCAGTGCCCGCGTGCTGATGGAAGGTTGTGTGCGGGTGCCAGCAGAAAGTGTGCAGAACGTGGATTGA

Upstream 100 bases:

>100_bases
GGAGTCCTTCTCAATGATGTGAATGTGGATAGTCCTACACAGGTCGGCTGAGTGGGCACTGTCTTTCCCTCTTTTCCCTT
ACCGCGTTCGAGGTCTTGTC

Downstream 100 bases:

>100_bases
GTTTTGATCCCGATTGCATGTGTTGCTGTCTTGGGGTGCTGTTAGGTGTTTGTGTGCATGTCGTGTGTTCGCCGCTATCA
CCTTGGGGTGGCTCTACATA

Product: AcnD-accessory protein PrpF

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MLPLPQCRIPATYLRGGTSKGVFFRLEDLPPACRVPGPARDALLLRVIGSPDPYGKQIDGMGGGTSSTSKVVIIAPSAYP
NHDVDYLFGQVAIEGACVDWTGNCGNLSAAVGPFAIAAGIVDPARVPHRGICSVRIWQANIGKTIVAHVPILDGQVQESG
DFMMEGAAFPGAEVRLEFLNPADVGGDRVMFPTGHLIDTLDVPGLGPIEATLINAGIPMLCVDAHALGYTGTELQEAING
DPHALVCLETLRAYGALRMGVIGHLEDMVQHQHTPKLAFVAAPADYVASGGRHVHATEIDVLVRAMSMGRLHHAMMGTAA
VALGTAAAIPGTLVNRAAGGGLRRVVRFGHPSGTLQVGAEVHQVNGQWSVANVRMSRSARVLMEGCVRVPAESVQNVD

Sequences:

>Translated_398_residues
MLPLPQCRIPATYLRGGTSKGVFFRLEDLPPACRVPGPARDALLLRVIGSPDPYGKQIDGMGGGTSSTSKVVIIAPSAYP
NHDVDYLFGQVAIEGACVDWTGNCGNLSAAVGPFAIAAGIVDPARVPHRGICSVRIWQANIGKTIVAHVPILDGQVQESG
DFMMEGAAFPGAEVRLEFLNPADVGGDRVMFPTGHLIDTLDVPGLGPIEATLINAGIPMLCVDAHALGYTGTELQEAING
DPHALVCLETLRAYGALRMGVIGHLEDMVQHQHTPKLAFVAAPADYVASGGRHVHATEIDVLVRAMSMGRLHHAMMGTAA
VALGTAAAIPGTLVNRAAGGGLRRVVRFGHPSGTLQVGAEVHQVNGQWSVANVRMSRSARVLMEGCVRVPAESVQNVD
>Mature_398_residues
MLPLPQCRIPATYLRGGTSKGVFFRLEDLPPACRVPGPARDALLLRVIGSPDPYGKQIDGMGGGTSSTSKVVIIAPSAYP
NHDVDYLFGQVAIEGACVDWTGNCGNLSAAVGPFAIAAGIVDPARVPHRGICSVRIWQANIGKTIVAHVPILDGQVQESG
DFMMEGAAFPGAEVRLEFLNPADVGGDRVMFPTGHLIDTLDVPGLGPIEATLINAGIPMLCVDAHALGYTGTELQEAING
DPHALVCLETLRAYGALRMGVIGHLEDMVQHQHTPKLAFVAAPADYVASGGRHVHATEIDVLVRAMSMGRLHHAMMGTAA
VALGTAAAIPGTLVNRAAGGGLRRVVRFGHPSGTLQVGAEVHQVNGQWSVANVRMSRSARVLMEGCVRVPAESVQNVD

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=385, Percent_Identity=33.7662337662338, Blast_Score=157, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 41719; Mature: 41719

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLPQCRIPATYLRGGTSKGVFFRLEDLPPACRVPGPARDALLLRVIGSPDPYGKQIDG
CCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECC
MGGGTSSTSKVVIIAPSAYPNHDVDYLFGQVAIEGACVDWTGNCGNLSAAVGPFAIAAGI
CCCCCCCCCEEEEEECCCCCCCCHHHHEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCC
VDPARVPHRGICSVRIWQANIGKTIVAHVPILDGQVQESGDFMMEGAAFPGAEVRLEFLN
CCCCCCCCCCCEEEEEEECCCCCEEEEEECEECCCCCCCCCEEEECCCCCCCEEEEEEEC
PADVGGDRVMFPTGHLIDTLDVPGLGPIEATLINAGIPMLCVDAHALGYTGTELQEAING
CCCCCCCEEECCCCCEEECCCCCCCCCHHHEEECCCCCEEEEECHHCCCCCHHHHHHHCC
DPHALVCLETLRAYGALRMGVIGHLEDMVQHQHTPKLAFVAAPADYVASGGRHVHATEID
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEHHHHH
VLVRAMSMGRLHHAMMGTAAVALGTAAAIPGTLVNRAAGGGLRRVVRFGHPSGTLQVGAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCCCCCEEECCE
VHQVNGQWSVANVRMSRSARVLMEGCVRVPAESVQNVD
EEECCCCEEEEEEEHHHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MLPLPQCRIPATYLRGGTSKGVFFRLEDLPPACRVPGPARDALLLRVIGSPDPYGKQIDG
CCCCCCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECC
MGGGTSSTSKVVIIAPSAYPNHDVDYLFGQVAIEGACVDWTGNCGNLSAAVGPFAIAAGI
CCCCCCCCCEEEEEECCCCCCCCHHHHEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHCC
VDPARVPHRGICSVRIWQANIGKTIVAHVPILDGQVQESGDFMMEGAAFPGAEVRLEFLN
CCCCCCCCCCCEEEEEEECCCCCEEEEEECEECCCCCCCCCEEEECCCCCCCEEEEEEEC
PADVGGDRVMFPTGHLIDTLDVPGLGPIEATLINAGIPMLCVDAHALGYTGTELQEAING
CCCCCCCEEECCCCCEEECCCCCCCCCHHHEEECCCCCEEEEECHHCCCCCHHHHHHHCC
DPHALVCLETLRAYGALRMGVIGHLEDMVQHQHTPKLAFVAAPADYVASGGRHVHATEID
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHCCCCEEEHHHHH
VLVRAMSMGRLHHAMMGTAAVALGTAAAIPGTLVNRAAGGGLRRVVRFGHPSGTLQVGAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCCCCCEEECCE
VHQVNGQWSVANVRMSRSARVLMEGCVRVPAESVQNVD
EEECCCCEEEEEEEHHHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA