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The map label for this gene is mchE [H]

Identifier: 182681050

GI number: 182681050

Start: 591492

End: 592754

Strand: Direct

Name: mchE [H]

Synonym: XfasM23_0489

Alternate gene names: 182681050

Gene position: 591492-592754 (Clockwise)

Preceding gene: 182681049

Following gene: 182681051

Centisome position: 23.33

GC content: 48.22

Gene sequence:

>1263_bases
GTGAAGGATTTGTTTCGCAAAGAAGTACTTGAGGCAAAGCGCACTAGTTGGCTTGGTGGTATCTCTTTGATCCAACCAAT
TAAAGCATGGTTGTTAGTGTTTGCATCCAGCGTTATGGCATTGTTAGTTGTGGTTTTTCTCTGCGTAGGGACTTACACTC
GTAGATCCAGTGTAACGGGGCAGCTTGTTCCATCTAAAGGGCTTGTGACGGTAGTCGCACCTGCCACGGGTATGGTAAGC
CAGATAAAAGTGTCAGAGGGGGCTAATGCACTTTCTGGGCAGATACTCGCATTGGTTACAGTGCCGCGGACTACTCTTGC
CAGTGGTGATGCCATCAGAGCCTTGGAGCAACAGTTGCAGGAGCGGAAGCAGGGATTGTCGATGGGTCAACAAGCTCAGG
CGCAAGTGCTATCTGCACAAACATCAGGGATTGAAGCGCAACTGAGTGCTGCGCAACGTGAATTGGCACAAATCGAAATT
GAGATTGCGACTCGTCAGAAACAGATACAGATTGCAAACGAAACTTTGCAGCGTTTGAAGCAGTTAGAGGATGCGCGTTA
TGTGGGCACCCTCCAAATCAAACAACAGGAATCCACTGTACTGGATTACACGAGCCAGATGCAGGCAATGCAACGTCAAG
CTATCATGACGCGACGGTCTATTGCTCAGTTGCAACAATCTTTAAGGGAATTACCGGGGCAGTATCAGAATTCCCAGGCA
ACCTACAGACGTGAACTGGCGCAACTGGAGCAGGAAAAGGTAGAAACGGAGGCGCGCGGTGCGCTTTCGGTTGTTTCCCC
AATTGATGGAATCGTGGCTACCCAGATTGTCAAGTCTCAGCAAACAGTACAGGCGGGGCAGCCGATGTTGAGTCTGTTAC
CCGTGAATGGTGAGTTGGAAGCAGAACTTCTATTGCCGAGTAGCGCCATTGGTTTTATCGAGCAAGGGGATGAGGTCCGT
TTACGTTACCAAGCTTACCCATATCAGAAGTTCGGGCATCAACGTGGTCAAGTATCCAGAATTAGTCGTAGTGCGTTGAC
TTCGAGCGAGCTTGCAGCATTAATCGGTAATACTCAACAAACCGAACCGTATTACCGCGTGATAGTGAAGCTTCCGCAAC
AGTCCATCACAGTCTATGGTCGGCATGAACCATTAAAACCTGGGATGCTCATTGATGCTGATATTCTTAGCGAGAAACGT
CGATTAATAGAATGGATATTTGAACCATTGTACTCATTGCGAGGAAAGGTTAGTGATAATTGA

Upstream 100 bases:

>100_bases
CAACAGCGAAGAAATAATCAAATTAACGAAAATATGAGTTGCACTGTTGAAAGAGAAATTGTTATAAATCCACTAATGAA
AATTGTGATTTTAATCAATT

Downstream 100 bases:

>100_bases
TTTTTCTATTGATAGTATTGATCTTACCTTAAATATATAAGTTATATGTATTTTTTGATACTTTTTCATTATATTATTTA
TATTAATCTTGGCGAATGAA

Product: colicin V secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MKDLFRKEVLEAKRTSWLGGISLIQPIKAWLLVFASSVMALLVVVFLCVGTYTRRSSVTGQLVPSKGLVTVVAPATGMVS
QIKVSEGANALSGQILALVTVPRTTLASGDAIRALEQQLQERKQGLSMGQQAQAQVLSAQTSGIEAQLSAAQRELAQIEI
EIATRQKQIQIANETLQRLKQLEDARYVGTLQIKQQESTVLDYTSQMQAMQRQAIMTRRSIAQLQQSLRELPGQYQNSQA
TYRRELAQLEQEKVETEARGALSVVSPIDGIVATQIVKSQQTVQAGQPMLSLLPVNGELEAELLLPSSAIGFIEQGDEVR
LRYQAYPYQKFGHQRGQVSRISRSALTSSELAALIGNTQQTEPYYRVIVKLPQQSITVYGRHEPLKPGMLIDADILSEKR
RLIEWIFEPLYSLRGKVSDN

Sequences:

>Translated_420_residues
MKDLFRKEVLEAKRTSWLGGISLIQPIKAWLLVFASSVMALLVVVFLCVGTYTRRSSVTGQLVPSKGLVTVVAPATGMVS
QIKVSEGANALSGQILALVTVPRTTLASGDAIRALEQQLQERKQGLSMGQQAQAQVLSAQTSGIEAQLSAAQRELAQIEI
EIATRQKQIQIANETLQRLKQLEDARYVGTLQIKQQESTVLDYTSQMQAMQRQAIMTRRSIAQLQQSLRELPGQYQNSQA
TYRRELAQLEQEKVETEARGALSVVSPIDGIVATQIVKSQQTVQAGQPMLSLLPVNGELEAELLLPSSAIGFIEQGDEVR
LRYQAYPYQKFGHQRGQVSRISRSALTSSELAALIGNTQQTEPYYRVIVKLPQQSITVYGRHEPLKPGMLIDADILSEKR
RLIEWIFEPLYSLRGKVSDN
>Mature_420_residues
MKDLFRKEVLEAKRTSWLGGISLIQPIKAWLLVFASSVMALLVVVFLCVGTYTRRSSVTGQLVPSKGLVTVVAPATGMVS
QIKVSEGANALSGQILALVTVPRTTLASGDAIRALEQQLQERKQGLSMGQQAQAQVLSAQTSGIEAQLSAAQRELAQIEI
EIATRQKQIQIANETLQRLKQLEDARYVGTLQIKQQESTVLDYTSQMQAMQRQAIMTRRSIAQLQQSLRELPGQYQNSQA
TYRRELAQLEQEKVETEARGALSVVSPIDGIVATQIVKSQQTVQAGQPMLSLLPVNGELEAELLLPSSAIGFIEQGDEVR
LRYQAYPYQKFGHQRGQVSRISRSALTSSELAALIGNTQQTEPYYRVIVKLPQQSITVYGRHEPLKPGMLIDADILSEKR
RLIEWIFEPLYSLRGKVSDN

Specific function: Probably involved, in conjunction with mchF, in the secretion of microcin H47 [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 46514; Mature: 46514

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDLFRKEVLEAKRTSWLGGISLIQPIKAWLLVFASSVMALLVVVFLCVGTYTRRSSVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCC
QLVPSKGLVTVVAPATGMVSQIKVSEGANALSGQILALVTVPRTTLASGDAIRALEQQLQ
EECCCCCCEEEEECCCCCHHHEECCCCCCCCCCCEEEEEEECCHHHCCCHHHHHHHHHHH
ERKQGLSMGQQAQAQVLSAQTSGIEAQLSAAQRELAQIEIEIATRQKQIQIANETLQRLK
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHH
QLEDARYVGTLQIKQQESTVLDYTSQMQAMQRQAIMTRRSIAQLQQSLRELPGQYQNSQA
HHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
TYRRELAQLEQEKVETEARGALSVVSPIDGIVATQIVKSQQTVQAGQPMLSLLPVNGELE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCE
AELLLPSSAIGFIEQGDEVRLRYQAYPYQKFGHQRGQVSRISRSALTSSELAALIGNTQQ
EEEECCCHHHHHHCCCCCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
TEPYYRVIVKLPQQSITVYGRHEPLKPGMLIDADILSEKRRLIEWIFEPLYSLRGKVSDN
CCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKDLFRKEVLEAKRTSWLGGISLIQPIKAWLLVFASSVMALLVVVFLCVGTYTRRSSVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCC
QLVPSKGLVTVVAPATGMVSQIKVSEGANALSGQILALVTVPRTTLASGDAIRALEQQLQ
EECCCCCCEEEEECCCCCHHHEECCCCCCCCCCCEEEEEEECCHHHCCCHHHHHHHHHHH
ERKQGLSMGQQAQAQVLSAQTSGIEAQLSAAQRELAQIEIEIATRQKQIQIANETLQRLK
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHH
QLEDARYVGTLQIKQQESTVLDYTSQMQAMQRQAIMTRRSIAQLQQSLRELPGQYQNSQA
HHHHCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
TYRRELAQLEQEKVETEARGALSVVSPIDGIVATQIVKSQQTVQAGQPMLSLLPVNGELE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCE
AELLLPSSAIGFIEQGDEVRLRYQAYPYQKFGHQRGQVSRISRSALTSSELAALIGNTQQ
EEEECCCHHHHHHCCCCCEEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
TEPYYRVIVKLPQQSITVYGRHEPLKPGMLIDADILSEKRRLIEWIFEPLYSLRGKVSDN
CCHHHHHHHHCCCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11181394 [H]