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The map label for this gene is nrdF

Identifier: 182681036

GI number: 182681036

Start: 578310

End: 579359

Strand: Direct

Name: nrdF

Synonym: XfasM23_0475

Alternate gene names: 182681036

Gene position: 578310-579359 (Clockwise)

Preceding gene: 182681035

Following gene: 182681037

Centisome position: 22.81

GC content: 46.95

Gene sequence:

>1050_bases
ATGACCAACATGGCTATCTCACTAGATCGCATCAAGATTTTGGAACCCCGTAATCCTAATCGTTCCACAGGGATTATTAA
CGGGAAAACCAGCGGCATTCTGAATTGGAACGACATTCCTTACCCATCCTTCTATCGGGCGTACAAGGAATTGTCGACCA
ACTTTTGGATCCCGGACGAGGTTGATATGAAGATCGACAAGCGCCAATACGGTGAACTGTCGGCACGCGAGAAAAACGCT
TATGACGCCATCATCGGTTTGCTAGCTACATTGGATTCACCGCAGACACGCTTCATCTACAACGTTGCTGAGTACATCAC
TGATCCGGCGGCACATGCCAATGCTGCGATTATTGGTCAGCAAGAGGTGATTCACAACGAGAGCTACAGCTATGTGTTGG
CTTCCATCACTGACTTGGCCAACCAAAACCGTGTGTTTGAGATCGCTCGGACCCATCCCACCATTCTCAAGCGCAATGCC
CCAATCATGCAGGCCTATGACGATTTTATGCGTGAGAAAACTGCTGAGACTTTGCTGTATTCGCTGATTCAATCCTCGAT
TCTTGAGGGTATTAATTTTTATTCTGGCTTTGCCTATTTCTACAACTTGGTGCGGCAGAACCGCATGACTGGCACTGGCA
AAATCATCAGCTTTATCAACCGCGATGAATTGGCTCATACCAAATTCATCAGCGAAGTGATTCGTGCCATTGTTGGTGAG
AATCCGGAATTGCAAACCGATGCATTGACCGATTACGTGCATCAAGCATTTGCCCATGCGATTGAGCTAGAAACACAGTG
GACTGGTGAGGTACTTGATGGCATTGACGGTATTGACGTAGAGGAGATGATCCGCTACGTGAAGTACCGCGCTAATAAAA
TGGCTGGCATGCTTGGTATCCAGACGCTGTACACCGAGACTACTGATAACGTCATGCCCTGGATCAGGGCTTATGCAGAC
AATTTTACCGAGACAAAAACGGATTTCTTTGAGATGCGTAATGCCAGCTATAAGAAGACCAATGTAGACAACTGCTTTGA
CGACTTATAG

Upstream 100 bases:

>100_bases
ACATCAGCGAATGCGAGTGGTGTTCAAGCTGATGCTGTTTGCTCTGCCTGAATTTGTAGTCTATCGGTCCTGTGCCGGTG
TCCTGCATCGTTTGACTTAA

Downstream 100 bases:

>100_bases
CTGATGATGCCATGCGAATTCTGCTTGCTCTCGCCTCCCTAAGTGGTAACACACGTGAAGTGGCCCGTGTCATCAGGGCG
CGCTGTGAGGAGAGAGGTCA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTNMAISLDRIKILEPRNPNRSTGIINGKTSGILNWNDIPYPSFYRAYKELSTNFWIPDEVDMKIDKRQYGELSAREKNA
YDAIIGLLATLDSPQTRFIYNVAEYITDPAAHANAAIIGQQEVIHNESYSYVLASITDLANQNRVFEIARTHPTILKRNA
PIMQAYDDFMREKTAETLLYSLIQSSILEGINFYSGFAYFYNLVRQNRMTGTGKIISFINRDELAHTKFISEVIRAIVGE
NPELQTDALTDYVHQAFAHAIELETQWTGEVLDGIDGIDVEEMIRYVKYRANKMAGMLGIQTLYTETTDNVMPWIRAYAD
NFTETKTDFFEMRNASYKKTNVDNCFDDL

Sequences:

>Translated_349_residues
MTNMAISLDRIKILEPRNPNRSTGIINGKTSGILNWNDIPYPSFYRAYKELSTNFWIPDEVDMKIDKRQYGELSAREKNA
YDAIIGLLATLDSPQTRFIYNVAEYITDPAAHANAAIIGQQEVIHNESYSYVLASITDLANQNRVFEIARTHPTILKRNA
PIMQAYDDFMREKTAETLLYSLIQSSILEGINFYSGFAYFYNLVRQNRMTGTGKIISFINRDELAHTKFISEVIRAIVGE
NPELQTDALTDYVHQAFAHAIELETQWTGEVLDGIDGIDVEEMIRYVKYRANKMAGMLGIQTLYTETTDNVMPWIRAYAD
NFTETKTDFFEMRNASYKKTNVDNCFDDL
>Mature_348_residues
TNMAISLDRIKILEPRNPNRSTGIINGKTSGILNWNDIPYPSFYRAYKELSTNFWIPDEVDMKIDKRQYGELSAREKNAY
DAIIGLLATLDSPQTRFIYNVAEYITDPAAHANAAIIGQQEVIHNESYSYVLASITDLANQNRVFEIARTHPTILKRNAP
IMQAYDDFMREKTAETLLYSLIQSSILEGINFYSGFAYFYNLVRQNRMTGTGKIISFINRDELAHTKFISEVIRAIVGEN
PELQTDALTDYVHQAFAHAIELETQWTGEVLDGIDGIDVEEMIRYVKYRANKMAGMLGIQTLYTETTDNVMPWIRAYADN
FTETKTDFFEMRNASYKKTNVDNCFDDL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=316, Percent_Identity=23.4177215189873, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI289177074, Length=318, Percent_Identity=23.8993710691824, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI4557845, Length=308, Percent_Identity=22.7272727272727, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI260064013, Length=308, Percent_Identity=22.7272727272727, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788567, Length=337, Percent_Identity=23.7388724035608, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1789031, Length=310, Percent_Identity=24.1935483870968, Blast_Score=78, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322434, Length=310, Percent_Identity=23.5483870967742, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI18079279, Length=317, Percent_Identity=22.7129337539432, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40018; Mature: 39886

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNMAISLDRIKILEPRNPNRSTGIINGKTSGILNWNDIPYPSFYRAYKELSTNFWIPDE
CCCCEEEECCEEEECCCCCCCCCCEECCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCC
VDMKIDKRQYGELSAREKNAYDAIIGLLATLDSPQTRFIYNVAEYITDPAAHANAAIIGQ
CCCEECHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCEEEECH
QEVIHNESYSYVLASITDLANQNRVFEIARTHPTILKRNAPIMQAYDDFMREKTAETLLY
HHHHHCCCHHHHHHHHHHHHCCCCEEEEHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
SLIQSSILEGINFYSGFAYFYNLVRQNRMTGTGKIISFINRDELAHTKFISEVIRAIVGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPELQTDALTDYVHQAFAHAIELETQWTGEVLDGIDGIDVEEMIRYVKYRANKMAGMLGI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QTLYTETTDNVMPWIRAYADNFTETKTDFFEMRNASYKKTNVDNCFDDL
HHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TNMAISLDRIKILEPRNPNRSTGIINGKTSGILNWNDIPYPSFYRAYKELSTNFWIPDE
CCCEEEECCEEEECCCCCCCCCCEECCCCCCEECCCCCCCHHHHHHHHHHCCCCCCCCC
VDMKIDKRQYGELSAREKNAYDAIIGLLATLDSPQTRFIYNVAEYITDPAAHANAAIIGQ
CCCEECHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHCCEEEECH
QEVIHNESYSYVLASITDLANQNRVFEIARTHPTILKRNAPIMQAYDDFMREKTAETLLY
HHHHHCCCHHHHHHHHHHHHCCCCEEEEHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHH
SLIQSSILEGINFYSGFAYFYNLVRQNRMTGTGKIISFINRDELAHTKFISEVIRAIVGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPELQTDALTDYVHQAFAHAIELETQWTGEVLDGIDGIDVEEMIRYVKYRANKMAGMLGI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QTLYTETTDNVMPWIRAYADNFTETKTDFFEMRNASYKKTNVDNCFDDL
HHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]