Click here to switch to the map view.

The map label for this gene is tig

Identifier: 182681027

GI number: 182681027

Start: 563126

End: 564421

Strand: Direct

Name: tig

Synonym: XfasM23_0466

Alternate gene names: 182681027

Gene position: 563126-564421 (Clockwise)

Preceding gene: 182681025

Following gene: 182681028

Centisome position: 22.21

GC content: 49.23

Gene sequence:

>1296_bases
ATGCCAGTTTTGATCGAATCCATCGGTAATTTGGAGCGTCGTTTGACCTTCTCTGTGCCGGAAGACCGTTTGGAATCTCA
TGTTGATGAACGTTTACGTGAAATTGCTCGTGCCGCGCGTATTAATGGTTTCCGTGCTGGTAAGGTGCCAGCTAAAGTGA
TTGAGCAGCGCTTCGGTGAAAAGGTGCGTGCTGAGGTGCTTGATAGTTTATTGCGTGAGACTCTTGATTCTGCAATTCGT
GCGCATTCGTTGCGTCTAGCGGGTGCAGCCCGTATTGATCATGCTAACGAGGGGGGGCTTAATTTTGTCGCTACCTTCGA
AGTGGTGCCTGATTTTGGCGAGATTGATGTATCCAAGCTTGGGGTGGTTCGACGTACCGCCAAAGTGACCGACGTTGATA
TAGATCAAATGATCGAAAATTTGAGGTTGCAGCGGCGCACTTGGCGTGTCGCTGAGCATGGCGCTCAGGTGGGTTATCTG
GTGGCTCTGGAGACTTGGTCGCAAGCTGGTGATGAACGTTTGCCGATTGAAGGTGTCGAAGCCGGTAGCACCATTCTTGG
TTCCGGTGTGATGTTCGAGCAGATTGAGCGTGGTTTGGAAGGCTTATCTAAGGGAGATGAAAATGTGCTGGATGTAACTT
TTCCCGATGATTGGCGTGTAATGCAGCTTGCTGGTAAGGCTGTGCAAGTTCATGTCAAAGTGATTGAGGTTTCCGAGCCA
GTGTTGTTGGAGGTGAACGAAGAGTTCATCAAGAGTTTTGGGGTGAAGAGCGGAAAATTAGAGGATTTTCGTGCAGATAT
CCGTGCAAATCTAGAACGTGAATTGAAGGGGGCGCTAGTGAGTCATCTGCGTCGTGAGATCGGGGAGCAGTTAATTGCTG
CCTACGCTCATGTTGAAATGCCGCCTCGTTTGGTTGAGAAAGAGGCTCGCTTAATGTTAGCTAAGCAGATTGAGCAGATT
CGACTGAGTGGTCGTGACCCTACGGTTATCCCCGATGATGCACATATTGGTTTTATGGATGCTGCTTGCAAACGTGTTTT
GGTTGGCTTGTTGGTTGGTGAGATTGCTGGACGCAATCGATTGCGCTTGGATCCTATGCGTGTCACTGAAACGCTCCATT
TGATTGCTTCAACCTACGAGGAGCCCGAAGAGGTATTTCAAATGTACCGCAATGATCCCAAACTGATGGAGGGCGTACAG
AGCTTGGTTATGGAGGAGCAGGTGATCGAATGGATCGCTGACCGTGCACAGAAAACTGAACAAGTGCTGTCGTTCCAGGA
AGCTATCCAGCAATAA

Upstream 100 bases:

>100_bases
CTCCTATAGGTGAGGGCGGGCTGACTGCCTTTATCCGGTTCAACTACAATTTCACTAAAAATTCGTACTGGCCTAATGGG
TCGTCGAGGTAGGAGTCAAC

Downstream 100 bases:

>100_bases
ACTGGTTAGTTTTGCGATCATGTGTCTTCTTGATACATGAATATAGGCGCTTTTGTTCTACCCAATAACAAAGTTAGTTC
CTTGTGTACCTCTGTGTTGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPVLIESIGNLERRLTFSVPEDRLESHVDERLREIARAARINGFRAGKVPAKVIEQRFGEKVRAEVLDSLLRETLDSAIR
AHSLRLAGAARIDHANEGGLNFVATFEVVPDFGEIDVSKLGVVRRTAKVTDVDIDQMIENLRLQRRTWRVAEHGAQVGYL
VALETWSQAGDERLPIEGVEAGSTILGSGVMFEQIERGLEGLSKGDENVLDVTFPDDWRVMQLAGKAVQVHVKVIEVSEP
VLLEVNEEFIKSFGVKSGKLEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEMPPRLVEKEARLMLAKQIEQI
RLSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNRLRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQ
SLVMEEQVIEWIADRAQKTEQVLSFQEAIQQ

Sequences:

>Translated_431_residues
MPVLIESIGNLERRLTFSVPEDRLESHVDERLREIARAARINGFRAGKVPAKVIEQRFGEKVRAEVLDSLLRETLDSAIR
AHSLRLAGAARIDHANEGGLNFVATFEVVPDFGEIDVSKLGVVRRTAKVTDVDIDQMIENLRLQRRTWRVAEHGAQVGYL
VALETWSQAGDERLPIEGVEAGSTILGSGVMFEQIERGLEGLSKGDENVLDVTFPDDWRVMQLAGKAVQVHVKVIEVSEP
VLLEVNEEFIKSFGVKSGKLEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEMPPRLVEKEARLMLAKQIEQI
RLSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNRLRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQ
SLVMEEQVIEWIADRAQKTEQVLSFQEAIQQ
>Mature_430_residues
PVLIESIGNLERRLTFSVPEDRLESHVDERLREIARAARINGFRAGKVPAKVIEQRFGEKVRAEVLDSLLRETLDSAIRA
HSLRLAGAARIDHANEGGLNFVATFEVVPDFGEIDVSKLGVVRRTAKVTDVDIDQMIENLRLQRRTWRVAEHGAQVGYLV
ALETWSQAGDERLPIEGVEAGSTILGSGVMFEQIERGLEGLSKGDENVLDVTFPDDWRVMQLAGKAVQVHVKVIEVSEPV
LLEVNEEFIKSFGVKSGKLEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEMPPRLVEKEARLMLAKQIEQIR
LSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNRLRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQS
LVMEEQVIEWIADRAQKTEQVLSFQEAIQQ

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=437, Percent_Identity=33.6384439359268, Blast_Score=225, Evalue=3e-60,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_XYLF2 (B2I8K2)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829187.1
- ProteinModelPortal:   B2I8K2
- SMR:   B2I8K2
- GeneID:   6202281
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0466
- HOGENOM:   HBG635105
- OMA:   FEGRIRN
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 48472; Mature: 48341

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLIESIGNLERRLTFSVPEDRLESHVDERLREIARAARINGFRAGKVPAKVIEQRFGE
CCCHHHHHCCHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KVRAEVLDSLLRETLDSAIRAHSLRLAGAARIDHANEGGLNFVATFEVVPDFGEIDVSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHH
GVVRRTAKVTDVDIDQMIENLRLQRRTWRVAEHGAQVGYLVALETWSQAGDERLPIEGVE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHCCCCCCCCCCCC
AGSTILGSGVMFEQIERGLEGLSKGDENVLDVTFPDDWRVMQLAGKAVQVHVKVIEVSEP
CCCHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCEEEEEEEEEECCCC
VLLEVNEEFIKSFGVKSGKLEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEM
EEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPRLVEKEARLMLAKQIEQIRLSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
LRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQSLVMEEQVIEWIADRAQKTE
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVLSFQEAIQQ
HHHHHHHHHCC
>Mature Secondary Structure 
PVLIESIGNLERRLTFSVPEDRLESHVDERLREIARAARINGFRAGKVPAKVIEQRFGE
CCHHHHHCCHHHHEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
KVRAEVLDSLLRETLDSAIRAHSLRLAGAARIDHANEGGLNFVATFEVVPDFGEIDVSKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCHHHH
GVVRRTAKVTDVDIDQMIENLRLQRRTWRVAEHGAQVGYLVALETWSQAGDERLPIEGVE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHCCCCCCCCCCCC
AGSTILGSGVMFEQIERGLEGLSKGDENVLDVTFPDDWRVMQLAGKAVQVHVKVIEVSEP
CCCHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCEEEEEEEEEECCCC
VLLEVNEEFIKSFGVKSGKLEDFRADIRANLERELKGALVSHLRREIGEQLIAAYAHVEM
EEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PPRLVEKEARLMLAKQIEQIRLSGRDPTVIPDDAHIGFMDAACKRVLVGLLVGEIAGRNR
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
LRLDPMRVTETLHLIASTYEEPEEVFQMYRNDPKLMEGVQSLVMEEQVIEWIADRAQKTE
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVLSFQEAIQQ
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA