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The map label for this gene is murD

Identifier: 182680966

GI number: 182680966

Start: 504362

End: 505768

Strand: Direct

Name: murD

Synonym: XfasM23_0404

Alternate gene names: 182680966

Gene position: 504362-505768 (Clockwise)

Preceding gene: 182680965

Following gene: 182680967

Centisome position: 19.89

GC content: 59.56

Gene sequence:

>1407_bases
GTGCGCATTTCCGCACTTGAAGGCTGCCGTGTCGCGTTGTGGGGCTGGGGGCGCGAAAGTCGTGCCGCTTACCGTGCATT
TCGTGCCGCGTTTCCGAAACAACCATTAACGTTGTTCTGCACGATTGCCGAAGCGACTGAGGTGCAGGCGCTGGCTGATC
CGGTACTGTCCATTGAAACCGAAGTGAGTGTTCCACGGCTTGCCGCCTTCGATGTGGTGATTAAATCGCCAGGCATCAGT
CCTTACTCTCCGCTGGCTGTTGCTGCTACCGCCGCGGGAGCGCATTTCATTGGTGGCACCCAATTGTGGTTTGCAGCGCA
CGCCGATACGGATGGCGTGGTTCCTGGTGCCGTGTGCGTCACTGGGACTAAAGGCAAAAGCACGACAACGGCACTGCTGG
CGCACCTGTTGCGCGCTGCGGGGCATTGCACGGCATTGGCTGGCAACATTGGCCTGCCGTTACTGGAGTTGCTAACACCG
CAGCCGGCACCCGAGTATTGGGCCATCGAATTGTCCAGTTATCAGACCGCAGATGTGGCAGGCAGTGGGGCGCGTCCCGC
GCTGGCGTTGGTGTTGAATGTGTTTCCCGAGCACTTGGATTGGCATGGCGGCGAGCAGCGCTATATCAATGACAAGCTCA
GCCTGGTGACTGTGGCGCGTCCCCCCATCGCCTTGCTCAATGCCGCCGATCCGCGGTTGGCGTCGCTGGTATTGCCACAG
AGTGATCTGCGTTGGTTCAACCAGCGTGATGGCTGGCACGTGCGCGGTCAGGTGGTGTACCGGGGTGAGCAGGCCGTGCT
GGATACTGCATTAATTCCGTTGCCTGGAGCGCACAATGGCAGCAATGTCTGTGCAGTGTTGGCCACACTCGAAGCGCTGG
GGCTGAATGCGGTGGCCTTGGCACCTGCCGCCTGCAATTTCCGTCCGCTACCCAACCGGCTGCAATTTTTGGGGGAGCGC
GATGGCATTCTCTGGGTCAATGATTCGATCAGCACGACGCCGCATGCCACCTTGGCGGCGCTGGATTGCTTCCTCGGGCG
TCGCCGCGTCGCCGTATTGGTGGGTGGGTATGATCGGGGAGTGAATTGGGAATGTTTTGCTGCCCGGATAACACGGAAAG
TACCACTGGACATTGTCACGATGGGAGCCAACGGGCCACATATCCAGGCACTGCTGGCGCCATTGGCAGCAGGTCGATTT
GGATTACATGCGGCGGTGGATCTTCCGCATGCGGTGGCGTTGGCACGCACGGCGTTGGGTGCGCAGGGGGGGGTGTTATT
ACTTTCTCCTGGAGCACCGAGTTTTGGTGCTTATCAGGACTACGTGGAGCGAGGTCGCCACTTTGCTATTTTGGCGGGGT
TCGATCCGGAGGTGATCAGCAGCATTTCTGGTCTTGGCATTGCTTGA

Upstream 100 bases:

>100_bases
GAAATCCAGCCGCATCTGGATCCTGCACAGTTACAGTTAGACCCCTTGCTGACGTTGTCCGATGAGCATCGCATCCCAGC
ATCCCTATGGGAGTCCATGA

Downstream 100 bases:

>100_bases
GCTGAGGTCAGATAGATTGAAACGATGATGCTCCCGCCAAGCCAAGTGTTGCAGCATTGCCTGTTCACATGATCCGCACG
CATTTCATGCAGTCTTGCTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRISALEGCRVALWGWGRESRAAYRAFRAAFPKQPLTLFCTIAEATEVQALADPVLSIETEVSVPRLAAFDVVIKSPGIS
PYSPLAVAATAAGAHFIGGTQLWFAAHADTDGVVPGAVCVTGTKGKSTTTALLAHLLRAAGHCTALAGNIGLPLLELLTP
QPAPEYWAIELSSYQTADVAGSGARPALALVLNVFPEHLDWHGGEQRYINDKLSLVTVARPPIALLNAADPRLASLVLPQ
SDLRWFNQRDGWHVRGQVVYRGEQAVLDTALIPLPGAHNGSNVCAVLATLEALGLNAVALAPAACNFRPLPNRLQFLGER
DGILWVNDSISTTPHATLAALDCFLGRRRVAVLVGGYDRGVNWECFAARITRKVPLDIVTMGANGPHIQALLAPLAAGRF
GLHAAVDLPHAVALARTALGAQGGVLLLSPGAPSFGAYQDYVERGRHFAILAGFDPEVISSISGLGIA

Sequences:

>Translated_468_residues
MRISALEGCRVALWGWGRESRAAYRAFRAAFPKQPLTLFCTIAEATEVQALADPVLSIETEVSVPRLAAFDVVIKSPGIS
PYSPLAVAATAAGAHFIGGTQLWFAAHADTDGVVPGAVCVTGTKGKSTTTALLAHLLRAAGHCTALAGNIGLPLLELLTP
QPAPEYWAIELSSYQTADVAGSGARPALALVLNVFPEHLDWHGGEQRYINDKLSLVTVARPPIALLNAADPRLASLVLPQ
SDLRWFNQRDGWHVRGQVVYRGEQAVLDTALIPLPGAHNGSNVCAVLATLEALGLNAVALAPAACNFRPLPNRLQFLGER
DGILWVNDSISTTPHATLAALDCFLGRRRVAVLVGGYDRGVNWECFAARITRKVPLDIVTMGANGPHIQALLAPLAAGRF
GLHAAVDLPHAVALARTALGAQGGVLLLSPGAPSFGAYQDYVERGRHFAILAGFDPEVISSISGLGIA
>Mature_468_residues
MRISALEGCRVALWGWGRESRAAYRAFRAAFPKQPLTLFCTIAEATEVQALADPVLSIETEVSVPRLAAFDVVIKSPGIS
PYSPLAVAATAAGAHFIGGTQLWFAAHADTDGVVPGAVCVTGTKGKSTTTALLAHLLRAAGHCTALAGNIGLPLLELLTP
QPAPEYWAIELSSYQTADVAGSGARPALALVLNVFPEHLDWHGGEQRYINDKLSLVTVARPPIALLNAADPRLASLVLPQ
SDLRWFNQRDGWHVRGQVVYRGEQAVLDTALIPLPGAHNGSNVCAVLATLEALGLNAVALAPAACNFRPLPNRLQFLGER
DGILWVNDSISTTPHATLAALDCFLGRRRVAVLVGGYDRGVNWECFAARITRKVPLDIVTMGANGPHIQALLAPLAAGRF
GLHAAVDLPHAVALARTALGAQGGVLLLSPGAPSFGAYQDYVERGRHFAILAGFDPEVISSISGLGIA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=399, Percent_Identity=30.0751879699248, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005762 [H]

Pfam domain/function: PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 49316; Mature: 49316

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISALEGCRVALWGWGRESRAAYRAFRAAFPKQPLTLFCTIAEATEVQALADPVLSIET
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCHHEEEC
EVSVPRLAAFDVVIKSPGISPYSPLAVAATAAGAHFIGGTQLWFAAHADTDGVVPGAVCV
CCCCCCEEEEEEEEECCCCCCCCCHHHEEHHCCCEECCCCEEEEEEECCCCCCCCCEEEE
TGTKGKSTTTALLAHLLRAAGHCTALAGNIGLPLLELLTPQPAPEYWAIELSSYQTADVA
ECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCEEEEEECCCEEECCC
GSGARPALALVLNVFPEHLDWHGGEQRYINDKLSLVTVARPPIALLNAADPRLASLVLPQ
CCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCEEEEECCCCCCEEEECCH
SDLRWFNQRDGWHVRGQVVYRGEQAVLDTALIPLPGAHNGSNVCAVLATLEALGLNAVAL
HHHHHHHCCCCEEEEEEEEEECCHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEE
APAACNFRPLPNRLQFLGERDGILWVNDSISTTPHATLAALDCFLGRRRVAVLVGGYDRG
CCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCC
VNWECFAARITRKVPLDIVTMGANGPHIQALLAPLAAGRFGLHAAVDLPHAVALARTALG
CCCEEEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCC
AQGGVLLLSPGAPSFGAYQDYVERGRHFAILAGFDPEVISSISGLGIA
CCCCEEEECCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCC
>Mature Secondary Structure
MRISALEGCRVALWGWGRESRAAYRAFRAAFPKQPLTLFCTIAEATEVQALADPVLSIET
CCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHCCHHEEEC
EVSVPRLAAFDVVIKSPGISPYSPLAVAATAAGAHFIGGTQLWFAAHADTDGVVPGAVCV
CCCCCCEEEEEEEEECCCCCCCCCHHHEEHHCCCEECCCCEEEEEEECCCCCCCCCEEEE
TGTKGKSTTTALLAHLLRAAGHCTALAGNIGLPLLELLTPQPAPEYWAIELSSYQTADVA
ECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCCEEEEEECCCEEECCC
GSGARPALALVLNVFPEHLDWHGGEQRYINDKLSLVTVARPPIALLNAADPRLASLVLPQ
CCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCEEEEEECCCCEEEEECCCCCCEEEECCH
SDLRWFNQRDGWHVRGQVVYRGEQAVLDTALIPLPGAHNGSNVCAVLATLEALGLNAVAL
HHHHHHHCCCCEEEEEEEEEECCHHEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEE
APAACNFRPLPNRLQFLGERDGILWVNDSISTTPHATLAALDCFLGRRRVAVLVGGYDRG
CCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCC
VNWECFAARITRKVPLDIVTMGANGPHIQALLAPLAAGRFGLHAAVDLPHAVALARTALG
CCCEEEEEHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHCC
AQGGVLLLSPGAPSFGAYQDYVERGRHFAILAGFDPEVISSISGLGIA
CCCCEEEECCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]