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The map label for this gene is carA [H]

Identifier: 182680954

GI number: 182680954

Start: 484374

End: 485573

Strand: Direct

Name: carA [H]

Synonym: XfasM23_0392

Alternate gene names: 182680954

Gene position: 484374-485573 (Clockwise)

Preceding gene: 182680953

Following gene: 182680955

Centisome position: 19.1

GC content: 53.58

Gene sequence:

>1200_bases
GTGTTTGAGCTTTCCTGCAACCTGATTTCAGGCGGCGGCATCGGTGTCCCAGGCAGCCAGAGCGAGACTCAACCCGTGAC
AGAACACGCAATCCTAGTTCTCGAAGACGGTACCGTATTCGAGGGCGATGCTGTAGGCGCTAATGGGCTTTCCGTTGGTG
AGGTGGTATTTAACACAGCCCTCACTGGTTATCAGGAGATCCTGACCGATCCTTCGTATGCGTACCAATTGGTCACCCTG
ACTTATCCGCATATTGGCAATACGGGTTGTACCGATCAGGATGATGAGGCGAATAAGGTATGGGCGGCTGGGCTGATTGT
GCGGGATGTGCCGCGTCGTCCAAGCAATTGGCGCAGTCAGATTTCGTTGTCAGATTGGTTGGCCGCACGCGGTGTGGTTG
CTATTGCTGGGATCGATACGCGCAAACTGACCCGTATTCTTCGTGAGAAAGGCGCTCAGCATGGTGCGTTGATGGCTGGT
GAGATTGATGTTGGCAAGGCTCAGGATGCGGCTCATCAATTTGCAGGTATCAAAGGTATGGATTTAGCCAAAGTGGTCAG
TACCAAACAGGGGTACTCTTGGTACGAAGGCCAGCTTGATTTGGATCGTAACGAATGCAAGCGCGCCGCGCCGCAATATA
AGGTTGTGGCCTATGATTACGGTGTGAAGCTGAATATTCTGCGGATGTTGGCGGAGCGTGGTTGTGATCTTACAGTGGTG
CCTGCGCAGACTCCGGCTGACGAGGTGTTGGCGCTCTGCCCGGATGGGGTGTTTCTTTCCAATGGCCCAGGTGATCCAGA
GCCTTGTGATTATGCCGTTGCGGCGATTAAGACGTTCATCATGCGACGGGTTCCAATCTTCGGTATTTGTTTGGGTCATC
AATTGCTGGCCCAGGCTGTTGGTGCCAGGGTCGTCAAAATGAGCCATGGTCACCATGGTGCGAATCATCCGGTGCAGGAC
TTGCGCAGCGGGCGGGTAATGATTACGTCGCAGAATCATGGCTTTGCTGTTGACGAGGCAACGTTGCCAAACAATGTCAG
GGTGACGCATCGTTCGTTGTTCGACGGGACCAATCAGGGGATTGAGTTGCTTGATGTGCCGGCATTCTCGTTTCAAGGGC
ATCCGGAGGCTTCCCCTGGTCCGCACGATGTTGATGTGTTGTTTGACAGGTTCATTACAATGATGGCGGCTCAATCCTGA

Upstream 100 bases:

>100_bases
CCGTGTGTTGTCTGTGTGGTGATGTTGGGCTGGTACGGGGCGATGCTTGCCGTTAGAATAGCACTCTGCCCGAATCTAGT
TTCGGACCGGTCTCAACGCC

Downstream 100 bases:

>100_bases
CGGTTTTGATGTCCGTCATTAGTGATGTGCTGGTGGTGGTGGGGCGGTGTGCGTATCGGTATTGCCGTTGTCATTGGAAT
TACGATGCGATGTGAGATTC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MFELSCNLISGGGIGVPGSQSETQPVTEHAILVLEDGTVFEGDAVGANGLSVGEVVFNTALTGYQEILTDPSYAYQLVTL
TYPHIGNTGCTDQDDEANKVWAAGLIVRDVPRRPSNWRSQISLSDWLAARGVVAIAGIDTRKLTRILREKGAQHGALMAG
EIDVGKAQDAAHQFAGIKGMDLAKVVSTKQGYSWYEGQLDLDRNECKRAAPQYKVVAYDYGVKLNILRMLAERGCDLTVV
PAQTPADEVLALCPDGVFLSNGPGDPEPCDYAVAAIKTFIMRRVPIFGICLGHQLLAQAVGARVVKMSHGHHGANHPVQD
LRSGRVMITSQNHGFAVDEATLPNNVRVTHRSLFDGTNQGIELLDVPAFSFQGHPEASPGPHDVDVLFDRFITMMAAQS

Sequences:

>Translated_399_residues
MFELSCNLISGGGIGVPGSQSETQPVTEHAILVLEDGTVFEGDAVGANGLSVGEVVFNTALTGYQEILTDPSYAYQLVTL
TYPHIGNTGCTDQDDEANKVWAAGLIVRDVPRRPSNWRSQISLSDWLAARGVVAIAGIDTRKLTRILREKGAQHGALMAG
EIDVGKAQDAAHQFAGIKGMDLAKVVSTKQGYSWYEGQLDLDRNECKRAAPQYKVVAYDYGVKLNILRMLAERGCDLTVV
PAQTPADEVLALCPDGVFLSNGPGDPEPCDYAVAAIKTFIMRRVPIFGICLGHQLLAQAVGARVVKMSHGHHGANHPVQD
LRSGRVMITSQNHGFAVDEATLPNNVRVTHRSLFDGTNQGIELLDVPAFSFQGHPEASPGPHDVDVLFDRFITMMAAQS
>Mature_399_residues
MFELSCNLISGGGIGVPGSQSETQPVTEHAILVLEDGTVFEGDAVGANGLSVGEVVFNTALTGYQEILTDPSYAYQLVTL
TYPHIGNTGCTDQDDEANKVWAAGLIVRDVPRRPSNWRSQISLSDWLAARGVVAIAGIDTRKLTRILREKGAQHGALMAG
EIDVGKAQDAAHQFAGIKGMDLAKVVSTKQGYSWYEGQLDLDRNECKRAAPQYKVVAYDYGVKLNILRMLAERGCDLTVV
PAQTPADEVLALCPDGVFLSNGPGDPEPCDYAVAAIKTFIMRRVPIFGICLGHQLLAQAVGARVVKMSHGHHGANHPVQD
LRSGRVMITSQNHGFAVDEATLPNNVRVTHRSLFDGTNQGIELLDVPAFSFQGHPEASPGPHDVDVLFDRFITMMAAQS

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=374, Percent_Identity=39.3048128342246, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI21361331, Length=381, Percent_Identity=35.1706036745407, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI169790915, Length=381, Percent_Identity=35.1706036745407, Blast_Score=212, Evalue=4e-55,
Organism=Escherichia coli, GI1786215, Length=370, Percent_Identity=62.4324324324324, Blast_Score=474, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI193204318, Length=375, Percent_Identity=41.0666666666667, Blast_Score=250, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=380, Percent_Identity=37.8947368421053, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=388, Percent_Identity=36.5979381443299, Blast_Score=226, Evalue=5e-60,
Organism=Drosophila melanogaster, GI45555749, Length=381, Percent_Identity=38.3202099737533, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24642586, Length=381, Percent_Identity=38.3202099737533, Blast_Score=228, Evalue=5e-60,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 42958; Mature: 42958

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELSCNLISGGGIGVPGSQSETQPVTEHAILVLEDGTVFEGDAVGANGLSVGEVVFNTA
CEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCHHHHHHHHH
LTGYQEILTDPSYAYQLVTLTYPHIGNTGCTDQDDEANKVWAAGLIVRDVPRRPSNWRSQ
HHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHH
ISLSDWLAARGVVAIAGIDTRKLTRILREKGAQHGALMAGEIDVGKAQDAAHQFAGIKGM
CCHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCCCC
DLAKVVSTKQGYSWYEGQLDLDRNECKRAAPQYKVVAYDYGVKLNILRMLAERGCDLTVV
CHHHHHHCCCCCCEECCEECCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEE
PAQTPADEVLALCPDGVFLSNGPGDPEPCDYAVAAIKTFIMRRVPIFGICLGHQLLAQAV
CCCCCHHHHHEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GARVVKMSHGHHGANHPVQDLRSGRVMITSQNHGFAVDEATLPNNVRVTHRSLFDGTNQG
CCEEEEEECCCCCCCCCHHHHCCCEEEEEECCCCEEEECCCCCCCEEEEEHHHHCCCCCC
IELLDVPAFSFQGHPEASPGPHDVDVLFDRFITMMAAQS
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFELSCNLISGGGIGVPGSQSETQPVTEHAILVLEDGTVFEGDAVGANGLSVGEVVFNTA
CEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCEEECCCCCCCCCCHHHHHHHHH
LTGYQEILTDPSYAYQLVTLTYPHIGNTGCTDQDDEANKVWAAGLIVRDVPRRPSNWRSQ
HHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHH
ISLSDWLAARGVVAIAGIDTRKLTRILREKGAQHGALMAGEIDVGKAQDAAHQFAGIKGM
CCHHHHHHHCCEEEEECCCHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCCCC
DLAKVVSTKQGYSWYEGQLDLDRNECKRAAPQYKVVAYDYGVKLNILRMLAERGCDLTVV
CHHHHHHCCCCCCEECCEECCCHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEE
PAQTPADEVLALCPDGVFLSNGPGDPEPCDYAVAAIKTFIMRRVPIFGICLGHQLLAQAV
CCCCCHHHHHEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
GARVVKMSHGHHGANHPVQDLRSGRVMITSQNHGFAVDEATLPNNVRVTHRSLFDGTNQG
CCEEEEEECCCCCCCCCHHHHCCCEEEEEECCCCEEEECCCCCCCEEEEEHHHHCCCCCC
IELLDVPAFSFQGHPEASPGPHDVDVLFDRFITMMAAQS
EEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]