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The map label for this gene is rsmD [H]

Identifier: 182680832

GI number: 182680832

Start: 351062

End: 351688

Strand: Direct

Name: rsmD [H]

Synonym: XfasM23_0267

Alternate gene names: 182680832

Gene position: 351062-351688 (Clockwise)

Preceding gene: 182680830

Following gene: 182680833

Centisome position: 13.84

GC content: 58.53

Gene sequence:

>627_bases
ATGAATCATCGGCGTGTGTTACCGGTGCGTGTTTGTGGTGAAGGACATGTACGGATTATCGGTGGCCGCTGGCGGAATAC
GCGCTTGAGAGTGCCGGAACTGCCTGGGTTACGTCCGACCAGTAGCCGTGTGCGCGAAACGCTGTTCAATTGGTTGATGC
CTGTGTTGCCGGGGGCACATGTTCTGGATTTGTTTGCTGGGAGCGGTGCGTTGGGATTGGAAGCGGTGTCGCGTGGTGCG
GCGAGGGCGGTACTGGTCGAACGTCACGCGGAGTTGGTGGTCCTGTTGCGTGAGCAGGTGGCGCGGCTTGGTGCTCAGGA
TCAGGTGGAGATTGTGCAGGCTGATGCGTTGCAGTGGTTGCAACGGCCTGCTGCGGCGCGTTTCGATATTATCTTTCTTG
ATCCGCCGTTTGCTGCTGGGGTGTGGGAGACGGTGGTGGTGCGATTGGCGGGGCACGTGGCGGCCGCGGCGTGGTTGTAT
ATGGAGTCTTCGGTTGATCTGGAACCAGTGATGCTGCCAGGTTGGGAGTTGCATCGTGACGGTCGCACGCGTGAGGTGTG
CTATGCGTTGTATCGGTATCCGGCTGTTACACTGCCTGTTGGTTCTAAGTCAGTATCGTCTGTATGA

Upstream 100 bases:

>100_bases
TTTCAAGGTTTCTGGTCAGTCGGGAGGTATCATTGGGTTTCTTTTTTGGTGTCTGGCGGTCGATGTTCTTCTCTTGTCAT
CCGTTGTTTTTTGTTTGCCG

Downstream 100 bases:

>100_bases
GCGTGGTTAATCGCCGCATCGCCGTCTATCCTGGCACCTTCGATCCGATCACTAATGGTCACATTGATTTAGTGAGTCGT
GCTGCTCCGCTGTTTGAGTC

Product: methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNHRRVLPVRVCGEGHVRIIGGRWRNTRLRVPELPGLRPTSSRVRETLFNWLMPVLPGAHVLDLFAGSGALGLEAVSRGA
ARAVLVERHAELVVLLREQVARLGAQDQVEIVQADALQWLQRPAAARFDIIFLDPPFAAGVWETVVVRLAGHVAAAAWLY
MESSVDLEPVMLPGWELHRDGRTREVCYALYRYPAVTLPVGSKSVSSV

Sequences:

>Translated_208_residues
MNHRRVLPVRVCGEGHVRIIGGRWRNTRLRVPELPGLRPTSSRVRETLFNWLMPVLPGAHVLDLFAGSGALGLEAVSRGA
ARAVLVERHAELVVLLREQVARLGAQDQVEIVQADALQWLQRPAAARFDIIFLDPPFAAGVWETVVVRLAGHVAAAAWLY
MESSVDLEPVMLPGWELHRDGRTREVCYALYRYPAVTLPVGSKSVSSV
>Mature_208_residues
MNHRRVLPVRVCGEGHVRIIGGRWRNTRLRVPELPGLRPTSSRVRETLFNWLMPVLPGAHVLDLFAGSGALGLEAVSRGA
ARAVLVERHAELVVLLREQVARLGAQDQVEIVQADALQWLQRPAAARFDIIFLDPPFAAGVWETVVVRLAGHVAAAAWLY
MESSVDLEPVMLPGWELHRDGRTREVCYALYRYPAVTLPVGSKSVSSV

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=187, Percent_Identity=44.9197860962567, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 23036; Mature: 23036

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHRRVLPVRVCGEGHVRIIGGRWRNTRLRVPELPGLRPTSSRVRETLFNWLMPVLPGAH
CCCCEEEEEEEECCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VLDLFAGSGALGLEAVSRGAARAVLVERHAELVVLLREQVARLGAQDQVEIVQADALQWL
EEHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QRPAAARFDIIFLDPPFAAGVWETVVVRLAGHVAAAAWLYMESSVDLEPVMLPGWELHRD
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEECCC
GRTREVCYALYRYPAVTLPVGSKSVSSV
CCHHHHHHHHHHCCEEEECCCCCCCCCC
>Mature Secondary Structure
MNHRRVLPVRVCGEGHVRIIGGRWRNTRLRVPELPGLRPTSSRVRETLFNWLMPVLPGAH
CCCCEEEEEEEECCCCEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
VLDLFAGSGALGLEAVSRGAARAVLVERHAELVVLLREQVARLGAQDQVEIVQADALQWL
EEHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
QRPAAARFDIIFLDPPFAAGVWETVVVRLAGHVAAAAWLYMESSVDLEPVMLPGWELHRD
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCEEECCC
GRTREVCYALYRYPAVTLPVGSKSVSSV
CCHHHHHHHHHHCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]