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The map label for this gene is dctA

Identifier: 182680829

GI number: 182680829

Start: 344935

End: 346284

Strand: Direct

Name: dctA

Synonym: XfasM23_0264

Alternate gene names: 182680829

Gene position: 344935-346284 (Clockwise)

Preceding gene: 182680826

Following gene: 182680830

Centisome position: 13.6

GC content: 49.78

Gene sequence:

>1350_bases
ATGCATCCCAGCTCTCGGGCCAATGGGCCTGCCCCGCATAAACCTTATAATCCTTTTTATTTACAGTTGTATTTCTGGGT
GATCATCGCGATTATCCTGGGGGCGCTGTTAGGCCATTGTTATCCTGCTGTGGGTCAGCAACTCAAGCCGTTGGGGGATG
CCTTTATCAAGCTGGTGAAGATGATTATCTCGCCAGTGATTTTTCTGACAATCGTGACAGGGATCGCTAGCGTTGCGCAT
GTGGGGACGGTGGCTCGCGTATTTGGTAAGGCGATGGTGTATTTCCTTTTCTTTTCCACGCTAGCCTTGCTGCTGGGTTT
GGTCGTTGCTCATGTGGTGCATCCAGGTGCGGGCATGAACATTAATCCAGTAGATCTCCATCAGGGGGAGATCGCAAATT
ATGTGGAGAAATCACACGATTTGACGTTGGTTGGCTTTTTGATGGACATCATCCCGAAGACACTGTTGAGTCCGTTTGTT
GGGGACAATATTTTGCAGGTGTTGTTTGTGGCAGTGTTGTTCGGTATTGCGTTGGCGCTTGCTGGGGAACGTGGTAAGCC
TGTGCTCAATTTACTGGATGCGTTGACCGTACCAGTATTCAAGCTTGTCCAGATGTTGATGAAAATGGCTCCTATCGGTG
CATTCGGAGCGATTGCGTTCACGATTGGTAAGTATGGAGTTGATTCATTGGTGAATTTGGGGGGGTTGGTTGGCTCGTTC
TATCTCACTTCGCTGTTGTTTGTGTTGGTGATTTTGGGTGCCGTGAGTTGGTTGTGTGGTTTTTCAATATTAAAACTGAT
TCGCTATTTGAAGGCGGAGCTGTTGCTGGTGTTGGGGACTTCGTCTTCGGAGTCGGCGTTGCCGTCGCTAATGGAAAAGA
TGGTGCAGGCGGGGTGTGGAAAGTCCGTGGTTGGACTGGTTGTGCCCACTGGTTACTCTTTTAATCTGGATGGCACCAAT
ATTTATATGACGTTAGCGGCGTTGTTTATTGCTCAGGCAACCAACACGGAGCTGACGCCAGCGCATCAGCTTGCTTTGTT
TTTGGTGGCGATGTTGAGTTCTAAAGGGGCAGCCGGTGTGTCCGGTGCTGGTTTTATTACTTTGGCAGCAACGTTGGCGG
TGGTGCCGGAAGTGCCGATTGCGGGCATGGCACTAATCTTGGGTGTAGATCGCTTTATGAGTGAGTGCCGCTCACTGACC
AATTTTATTGGCAATGCAGTGGCTACGCTGGTAGTTTCGCGTTGGGAGAATGCGTTGAACTATGAGCAGCTCAAAATTGC
ACTGGATGGCAGTGAAGCTGCATACCAGTCACTACATGCCAATGATGCTGAGCCGTCTGTGTCGCGTTGA

Upstream 100 bases:

>100_bases
AGTACCAATAGGTTATCTGTATGATTACGTCTTCGTTCGAGAATTGCATCATTTGAAGCCCGCGCTATGCGGTTCCGTTT
TGTCTACCGGAGTTTGTTTC

Downstream 100 bases:

>100_bases
GTCTTTAGTTAGTCATCGCAGTGCGTGTTGCTGCGATGGTGGTGTTATACGCTTTGGGGTTTGTGGTGCCGTATTTAGCA
TCGGCTGTCACTGTTGCGTG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MHPSSRANGPAPHKPYNPFYLQLYFWVIIAIILGALLGHCYPAVGQQLKPLGDAFIKLVKMIISPVIFLTIVTGIASVAH
VGTVARVFGKAMVYFLFFSTLALLLGLVVAHVVHPGAGMNINPVDLHQGEIANYVEKSHDLTLVGFLMDIIPKTLLSPFV
GDNILQVLFVAVLFGIALALAGERGKPVLNLLDALTVPVFKLVQMLMKMAPIGAFGAIAFTIGKYGVDSLVNLGGLVGSF
YLTSLLFVLVILGAVSWLCGFSILKLIRYLKAELLLVLGTSSSESALPSLMEKMVQAGCGKSVVGLVVPTGYSFNLDGTN
IYMTLAALFIAQATNTELTPAHQLALFLVAMLSSKGAAGVSGAGFITLAATLAVVPEVPIAGMALILGVDRFMSECRSLT
NFIGNAVATLVVSRWENALNYEQLKIALDGSEAAYQSLHANDAEPSVSR

Sequences:

>Translated_449_residues
MHPSSRANGPAPHKPYNPFYLQLYFWVIIAIILGALLGHCYPAVGQQLKPLGDAFIKLVKMIISPVIFLTIVTGIASVAH
VGTVARVFGKAMVYFLFFSTLALLLGLVVAHVVHPGAGMNINPVDLHQGEIANYVEKSHDLTLVGFLMDIIPKTLLSPFV
GDNILQVLFVAVLFGIALALAGERGKPVLNLLDALTVPVFKLVQMLMKMAPIGAFGAIAFTIGKYGVDSLVNLGGLVGSF
YLTSLLFVLVILGAVSWLCGFSILKLIRYLKAELLLVLGTSSSESALPSLMEKMVQAGCGKSVVGLVVPTGYSFNLDGTN
IYMTLAALFIAQATNTELTPAHQLALFLVAMLSSKGAAGVSGAGFITLAATLAVVPEVPIAGMALILGVDRFMSECRSLT
NFIGNAVATLVVSRWENALNYEQLKIALDGSEAAYQSLHANDAEPSVSR
>Mature_449_residues
MHPSSRANGPAPHKPYNPFYLQLYFWVIIAIILGALLGHCYPAVGQQLKPLGDAFIKLVKMIISPVIFLTIVTGIASVAH
VGTVARVFGKAMVYFLFFSTLALLLGLVVAHVVHPGAGMNINPVDLHQGEIANYVEKSHDLTLVGFLMDIIPKTLLSPFV
GDNILQVLFVAVLFGIALALAGERGKPVLNLLDALTVPVFKLVQMLMKMAPIGAFGAIAFTIGKYGVDSLVNLGGLVGSF
YLTSLLFVLVILGAVSWLCGFSILKLIRYLKAELLLVLGTSSSESALPSLMEKMVQAGCGKSVVGLVVPTGYSFNLDGTN
IYMTLAALFIAQATNTELTPAHQLALFLVAMLSSKGAAGVSGAGFITLAATLAVVPEVPIAGMALILGVDRFMSECRSLT
NFIGNAVATLVVSRWENALNYEQLKIALDGSEAAYQSLHANDAEPSVSR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family

Homologues:

Organism=Homo sapiens, GI169790839, Length=454, Percent_Identity=26.2114537444934, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4827012, Length=296, Percent_Identity=28.7162162162162, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI194239697, Length=454, Percent_Identity=24.8898678414097, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI40254478, Length=486, Percent_Identity=24.2798353909465, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI21314632, Length=397, Percent_Identity=25.4408060453401, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI66773030, Length=406, Percent_Identity=23.6453201970443, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI223468566, Length=256, Percent_Identity=30.46875, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI223468564, Length=254, Percent_Identity=30.3149606299213, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI262359914, Length=374, Percent_Identity=25.1336898395722, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI301601644, Length=148, Percent_Identity=29.7297297297297, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789947, Length=413, Percent_Identity=54.7215496368039, Blast_Score=476, Evalue=1e-136,
Organism=Escherichia coli, GI1790514, Length=416, Percent_Identity=37.9807692307692, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1788024, Length=414, Percent_Identity=23.9130434782609, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71996953, Length=447, Percent_Identity=28.1879194630872, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17537407, Length=421, Percent_Identity=26.603325415677, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=486, Percent_Identity=25.9259259259259, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71983099, Length=486, Percent_Identity=25.9259259259259, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=423, Percent_Identity=21.9858156028369, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI193206654, Length=423, Percent_Identity=22.4586288416076, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17541374, Length=396, Percent_Identity=21.2121212121212, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583025, Length=433, Percent_Identity=23.5565819861432, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI17137668, Length=433, Percent_Identity=23.5565819861432, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24583023, Length=433, Percent_Identity=23.5565819861432, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281360481, Length=442, Percent_Identity=22.6244343891403, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281360483, Length=442, Percent_Identity=22.6244343891403, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17137666, Length=442, Percent_Identity=22.6244343891403, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DCTA_XYLF2 (B2I7I7)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828989.1
- ProteinModelPortal:   B2I7I7
- SMR:   B2I7I7
- GeneID:   6203660
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0264
- HOGENOM:   HBG739804
- OMA:   NAVACIV
- ProtClustDB:   PRK01663
- HAMAP:   MF_01300
- InterPro:   IPR001991
- InterPro:   IPR018107
- PANTHER:   PTHR11958
- PRINTS:   PR00173

Pfam domain/function: PF00375 SDF

EC number: NA

Molecular weight: Translated: 47631; Mature: 47631

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x132a9704)-; HASH(0x127cab8c)-; HASH(0x1388b7c0)-; HASH(0x1388e058)-; HASH(0x12deca74)-; HASH(0x12729a10)-; HASH(0x13697a48)-; HASH(0x13884894)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHEEEEECCCHHHHHHHHCCCCCCCCCH
>Mature Secondary Structure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HHHEEEEECCCHHHHHHHHCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA