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The map label for this gene is tpiA

Identifier: 182680803

GI number: 182680803

Start: 316138

End: 316887

Strand: Direct

Name: tpiA

Synonym: XfasM23_0238

Alternate gene names: 182680803

Gene position: 316138-316887 (Clockwise)

Preceding gene: 182680802

Following gene: 182680804

Centisome position: 12.47

GC content: 54.53

Gene sequence:

>750_bases
ATGCGTCCCAAGATTGTGGCTGGGAATTGGAAGTTGCATGGTAGCCACGCGTTCGCACAGGCATTGGTTGCGCAGGTCGC
TGCTGGTTTGCCGCTGCCTGGAGTCAGTGTGATTATCTTGCCGCCATTGCTGTATCTGAGTGATTTGGCGCAGCGCTTCA
AAGGGGAAGGTTTGGCGTTTGGTGCGCAGAACGTCAGTCACCATGATAAAGGTGCTTACACTGGGGAAGTTTCGGCGGCG
ATGGTGGCTGATGTCGGTGCTCATTACACTTTGGTTGGGCATTCTGAACGTCGGGAGTATCACCATGAGGACAGTGAGTT
GGTGGCGCGTAAATTTGCTGCTGCTCTGAGTGCTGGGTTGCGACCAATTCTATGTGTTGGGGAGAGTTTGCCCCAGCGTG
AGGCTGGACAGGCAGAGGTTGCCATTGCGATGCAGCTTGCGCCTGTGTTGGCTCTGGTTGGCCCGCAGGGTGTTGCCCGT
GGCTTGATTGCCTATGAGCCGGTTTGGGCGATTGGTACTGGCCGTCATGCGGATCCGAGCCAAGTTCAAGCGATGCACGC
TTTTATTCGTGGTGAAATTGCGAGGCAAGATGCTAGAATCGGCGATTCTCTGTTGATTCTATACGGAGGCGGCATCAAGC
CTTGCAATGCTGCTGAGTTGTTTTCCCAGCAGGATGTCGATGGTGGGTTGATTGGTGGTGCTTCGTTGGTTGCTGATGAT
TTCTTGGCTATCGCTCGTGCGACGGTTTAG

Upstream 100 bases:

>100_bases
GCTGGGGAGGGGCTGTGATGTGAATTGGATAGGCATTGTTCAGAAGACGATGGAACATCGGCTATCCTTGCGTACTGGAA
TTTTCAGGAGTTTCTAACCT

Downstream 100 bases:

>100_bases
GGCTGATCTGCAACGTTCCTTTACTCGGAGGCTGATTCGAGATGTTGTTGCTTAATGTGATCTATGTTCTTGTTGCCATC
GCTATGATTGTGTTGATTTT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRPKIVAGNWKLHGSHAFAQALVAQVAAGLPLPGVSVIILPPLLYLSDLAQRFKGEGLAFGAQNVSHHDKGAYTGEVSAA
MVADVGAHYTLVGHSERREYHHEDSELVARKFAAALSAGLRPILCVGESLPQREAGQAEVAIAMQLAPVLALVGPQGVAR
GLIAYEPVWAIGTGRHADPSQVQAMHAFIRGEIARQDARIGDSLLILYGGGIKPCNAAELFSQQDVDGGLIGGASLVADD
FLAIARATV

Sequences:

>Translated_249_residues
MRPKIVAGNWKLHGSHAFAQALVAQVAAGLPLPGVSVIILPPLLYLSDLAQRFKGEGLAFGAQNVSHHDKGAYTGEVSAA
MVADVGAHYTLVGHSERREYHHEDSELVARKFAAALSAGLRPILCVGESLPQREAGQAEVAIAMQLAPVLALVGPQGVAR
GLIAYEPVWAIGTGRHADPSQVQAMHAFIRGEIARQDARIGDSLLILYGGGIKPCNAAELFSQQDVDGGLIGGASLVADD
FLAIARATV
>Mature_249_residues
MRPKIVAGNWKLHGSHAFAQALVAQVAAGLPLPGVSVIILPPLLYLSDLAQRFKGEGLAFGAQNVSHHDKGAYTGEVSAA
MVADVGAHYTLVGHSERREYHHEDSELVARKFAAALSAGLRPILCVGESLPQREAGQAEVAIAMQLAPVLALVGPQGVAR
GLIAYEPVWAIGTGRHADPSQVQAMHAFIRGEIARQDARIGDSLLILYGGGIKPCNAAELFSQQDVDGGLIGGASLVADD
FLAIARATV

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=248, Percent_Identity=39.5161290322581, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI226529917, Length=237, Percent_Identity=40.084388185654, Blast_Score=161, Evalue=5e-40,
Organism=Escherichia coli, GI1790353, Length=249, Percent_Identity=49.3975903614458, Blast_Score=240, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI17536593, Length=250, Percent_Identity=39.6, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6320255, Length=239, Percent_Identity=41.0041841004184, Blast_Score=167, Evalue=1e-42,
Organism=Drosophila melanogaster, GI28572008, Length=236, Percent_Identity=38.9830508474576, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28572006, Length=236, Percent_Identity=38.9830508474576, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=38.4615384615385, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_XYLF2 (B2I782)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828963.1
- ProteinModelPortal:   B2I782
- SMR:   B2I782
- GeneID:   6202280
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0238
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26060; Mature: 26060

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPKIVAGNWKLHGSHAFAQALVAQVAAGLPLPGVSVIILPPLLYLSDLAQRFKGEGLAF
CCCCEEECCEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
GAQNVSHHDKGAYTGEVSAAMVADVGAHYTLVGHSERREYHHEDSELVARKFAAALSAGL
CCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCC
RPILCVGESLPQREAGQAEVAIAMQLAPVLALVGPQGVARGLIAYEPVWAIGTGRHADPS
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHCCCEEEECCCCCCCHH
QVQAMHAFIRGEIARQDARIGDSLLILYGGGIKPCNAAELFSQQDVDGGLIGGASLVADD
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEECCHHHHHHH
FLAIARATV
HHHHHHHCC
>Mature Secondary Structure
MRPKIVAGNWKLHGSHAFAQALVAQVAAGLPLPGVSVIILPPLLYLSDLAQRFKGEGLAF
CCCCEEECCEEECCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
GAQNVSHHDKGAYTGEVSAAMVADVGAHYTLVGHSERREYHHEDSELVARKFAAALSAGL
CCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHCC
RPILCVGESLPQREAGQAEVAIAMQLAPVLALVGPQGVARGLIAYEPVWAIGTGRHADPS
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCHHHCCCEEEECCCCCCCHH
QVQAMHAFIRGEIARQDARIGDSLLILYGGGIKPCNAAELFSQQDVDGGLIGGASLVADD
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCEECCHHHHHHH
FLAIARATV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA