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The map label for this gene is rfbB [H]

Identifier: 182680766

GI number: 182680766

Start: 265284

End: 266345

Strand: Direct

Name: rfbB [H]

Synonym: XfasM23_0195

Alternate gene names: 182680766

Gene position: 265284-266345 (Clockwise)

Preceding gene: 182680763

Following gene: 182680767

Centisome position: 10.46

GC content: 56.03

Gene sequence:

>1062_bases
ATGGGATTGGCAACGTGGCTCGTGACCGGTGGTGCCGGTTTCATCGGCGGTAATTTTGTATTGCAAGCGATTAGCCTCGG
GCATCGTGTGATTAATCTTGATGCTCTGACGTATGCGGGTCATTTGGAGACGCTCGCTGCGCTTGATGGGTGCGCGGAGC
ATCTTTTTGTTCATGGTGATATTGGCGACCGTGCGTTGGTGAGCCGTTTGCTGGATACGCATCGCCCGGATGCAGTGCTG
AATTTTGCTGCTGAGAGTCACGTTGATCGTTCGATTGATGCTCCCGCTGCGTTCATTCAGACCAATGTGGTTGGCACGTT
GCTGTTGTTGGAGGCGGTGTGTGGGTACTGGAAGGCGTTGCCGGAGGCGGCGCGTGCTGCGTTCCGTTTTCTGCATGTAT
CCACAGATGAGGTTTACGGCAGTTTGGGGGAGACGGGCGCATTTACAGAGAGGACGGCGTATGCACCTAATTCGCCTTAT
TCGGCGTCAAAGGCCGCGTCTGATCATTTGGTCCGTGCGTTTCACCATACGTACGGATTGCCGGTGCTGACCACGCATTG
TTCAAATAACTATGGGCCATATCATTTTCCGGAGAAGCTGATTCCGCTGGTCATCACCAAGGCGTTGGCGGGTGAGCCGT
TACCTGTGTATGGCGATGGGCGGCACGTGCGTGATTGGCTGTTTGTCGGCGATCACTGTACCGCGCTGTGTGCTGTATTG
GCTGGAGGGCGGGTGGGTGAAACGTACAATATCGGGGGGCAGGCCGAGCGTGAGAATCTTGAAGTGGTCCGCATGATTTG
CCGTTTGCTTGACGAGCGCCGCCCGCGTGCCGATGGGCTGAGTTATCAAAGCCAGATTGTTCATGTTGCAGATCGTCCGG
GGCATGATCGCCGTTATGCGATCGACACGTCCAAGTTGGTGAGTGAGTTAGGCTGGGCGCCGGTGTATCGCTTTGAGCAG
GGACTTGCGCTGACTGTGGATTGGTATTTGCAGCATCAGGATTGGGTACGTGCGGTGCTCCAAGGGCGTGACCGCGTTGA
GGGCCTACTGACTGCGGTGTAA

Upstream 100 bases:

>100_bases
GTGGCTGTTGTTGGATGTCAACCGTGGTGGGATGTGACCGGGGTATCCTATGGTGCTAGACGGTCCCTGTCAGGTGCAGG
GGTGTGGTGCTTGATGGAGA

Downstream 100 bases:

>100_bases
GGCCTTAGGTTCGGTAAAGAGTGCGTATCCATATGCAGACTTTCATATCAGGAAATCGTTCCATGACACAGCGCAAGGGC
ATCATCTTGGCGGGTGGGGC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MGLATWLVTGGAGFIGGNFVLQAISLGHRVINLDALTYAGHLETLAALDGCAEHLFVHGDIGDRALVSRLLDTHRPDAVL
NFAAESHVDRSIDAPAAFIQTNVVGTLLLLEAVCGYWKALPEAARAAFRFLHVSTDEVYGSLGETGAFTERTAYAPNSPY
SASKAASDHLVRAFHHTYGLPVLTTHCSNNYGPYHFPEKLIPLVITKALAGEPLPVYGDGRHVRDWLFVGDHCTALCAVL
AGGRVGETYNIGGQAERENLEVVRMICRLLDERRPRADGLSYQSQIVHVADRPGHDRRYAIDTSKLVSELGWAPVYRFEQ
GLALTVDWYLQHQDWVRAVLQGRDRVEGLLTAV

Sequences:

>Translated_353_residues
MGLATWLVTGGAGFIGGNFVLQAISLGHRVINLDALTYAGHLETLAALDGCAEHLFVHGDIGDRALVSRLLDTHRPDAVL
NFAAESHVDRSIDAPAAFIQTNVVGTLLLLEAVCGYWKALPEAARAAFRFLHVSTDEVYGSLGETGAFTERTAYAPNSPY
SASKAASDHLVRAFHHTYGLPVLTTHCSNNYGPYHFPEKLIPLVITKALAGEPLPVYGDGRHVRDWLFVGDHCTALCAVL
AGGRVGETYNIGGQAERENLEVVRMICRLLDERRPRADGLSYQSQIVHVADRPGHDRRYAIDTSKLVSELGWAPVYRFEQ
GLALTVDWYLQHQDWVRAVLQGRDRVEGLLTAV
>Mature_352_residues
GLATWLVTGGAGFIGGNFVLQAISLGHRVINLDALTYAGHLETLAALDGCAEHLFVHGDIGDRALVSRLLDTHRPDAVLN
FAAESHVDRSIDAPAAFIQTNVVGTLLLLEAVCGYWKALPEAARAAFRFLHVSTDEVYGSLGETGAFTERTAYAPNSPYS
ASKAASDHLVRAFHHTYGLPVLTTHCSNNYGPYHFPEKLIPLVITKALAGEPLPVYGDGRHVRDWLFVGDHCTALCAVLA
GGRVGETYNIGGQAERENLEVVRMICRLLDERRPRADGLSYQSQIVHVADRPGHDRRYAIDTSKLVSELGWAPVYRFEQG
LALTVDWYLQHQDWVRAVLQGRDRVEGLLTAV

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=34.6504559270517, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=25.5952380952381, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI4504031, Length=337, Percent_Identity=24.3323442136499, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788353, Length=346, Percent_Identity=56.6473988439306, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI48994969, Length=347, Percent_Identity=57.9250720461095, Blast_Score=388, Evalue=1e-109,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=27.6353276353276, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788366, Length=190, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=35.6287425149701, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=32.1533923303835, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17507723, Length=330, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=189, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=360, Percent_Identity=25.5555555555556, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71982038, Length=362, Percent_Identity=25.414364640884, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=294, Percent_Identity=25.1700680272109, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=25, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19923002, Length=295, Percent_Identity=29.4915254237288, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24158427, Length=283, Percent_Identity=26.5017667844523, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 38647; Mature: 38516

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLATWLVTGGAGFIGGNFVLQAISLGHRVINLDALTYAGHLETLAALDGCAEHLFVHGD
CCCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECC
IGDRALVSRLLDTHRPDAVLNFAAESHVDRSIDAPAAFIQTNVVGTLLLLEAVCGYWKAL
CCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PEAARAAFRFLHVSTDEVYGSLGETGAFTERTAYAPNSPYSASKAASDHLVRAFHHTYGL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PVLTTHCSNNYGPYHFPEKLIPLVITKALAGEPLPVYGDGRHVRDWLFVGDHCTALCAVL
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHEEECHHHHHHHHHH
AGGRVGETYNIGGQAERENLEVVRMICRLLDERRPRADGLSYQSQIVHVADRPGHDRRYA
HCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEECCCCCCCCEEE
IDTSKLVSELGWAPVYRFEQGLALTVDWYLQHQDWVRAVLQGRDRVEGLLTAV
ECHHHHHHHHCCCHHHHHCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
GLATWLVTGGAGFIGGNFVLQAISLGHRVINLDALTYAGHLETLAALDGCAEHLFVHGD
CCEEEEEECCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECC
IGDRALVSRLLDTHRPDAVLNFAAESHVDRSIDAPAAFIQTNVVGTLLLLEAVCGYWKAL
CCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PEAARAAFRFLHVSTDEVYGSLGETGAFTERTAYAPNSPYSASKAASDHLVRAFHHTYGL
HHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
PVLTTHCSNNYGPYHFPEKLIPLVITKALAGEPLPVYGDGRHVRDWLFVGDHCTALCAVL
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHEEECHHHHHHHHHH
AGGRVGETYNIGGQAERENLEVVRMICRLLDERRPRADGLSYQSQIVHVADRPGHDRRYA
HCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEEEECCCCCCCCEEE
IDTSKLVSELGWAPVYRFEQGLALTVDWYLQHQDWVRAVLQGRDRVEGLLTAV
ECHHHHHHHHCCCHHHHHCCCCEEEEEEEHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]