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The map label for this gene is truB

Identifier: 182680752

GI number: 182680752

Start: 247535

End: 248437

Strand: Direct

Name: truB

Synonym: XfasM23_0181

Alternate gene names: 182680752

Gene position: 247535-248437 (Clockwise)

Preceding gene: 182680751

Following gene: 182680753

Centisome position: 9.76

GC content: 51.83

Gene sequence:

>903_bases
ATGTCTCGCATTTCCTATCGTCGTTTGGATGGTATTTTGCTGCTTGATAAGCCAGCTGGCATAAGTTCTAACAGTGCGTT
ACAGGTCGCGCGTCGGTTATTGCGTGCTAAAAAGGGGGGGCATACTGGTAGCTTGGATCCACTCGCTACTGGATTGTTGC
CGCTGTGTTTTGGAGAAGCCACCAAAATCGCTGGTTTGCTACTGGATTCTACCAAGGCATACGACGCTGACGTGCTACTC
GGCACCACGACGACGACGGATGATGCTGAAGGGGCTGTATTACTTACGCGCCCTGTGCCGACGTTAGATGCCGAAATGGT
TGATGCTCTTATACCTCAATTGATTGGTCGGATTAGGCAACGTGCCCCAATCTATTCTGCTCTCAAACAGGGGGGTGAAC
CTTTATATGCTAAAGCTCGGCGTGGTGAAGTGGTCAATGCCCCGGTACGTGAGGTTGAGGTGCATGCGATCGAATTGCTT
TTTTTGGCTACGCCGCGATTACAATTACGTGTGACTTGCGGTTCGGGTACATATATTCGTAGCCTAGTTCGTGATTTGGG
CGAAATCTTGGGCTGCGGCGCACATATCAATGCATTGCGCCGTCGTTGGGTCGCTCCATTTCAGATACCGAAGATGTTGA
CGTTGGATGCATTGGAGCAGGCATTGAGTGCAGGGAGAGATCCTGATTCTCTGTTATTGCCATTGGTTGAAGGATTAGCC
GGTTTCCCAGCGCTGGAACTTGATGCCCAGCGTCTAGCTTGCTTCAGGCTAGGGCAGCGCTTGCGCGATCATAGTTTTCA
GACTGGGCGGGTTGCGGTGTTCAGTCGAGATGGTATCCCTGCGGGGCTGGGGGAGGTGGATAGTGAAGGCTTGTTGGTGC
CGCAGCGTCTTTTCAATCTTTGA

Upstream 100 bases:

>100_bases
TTAAACCTTGTATTGGTGAAATAACCAATTCAAGGTGTTGATCCTTTGTCCATAGTATGCTGTATTTGTTCGTTCTTCCC
GTGTTTGATGCTTCTTGCTT

Downstream 100 bases:

>100_bases
ATCTGGATTTTTTAAAAACTATCAGCTTGTGCCGAACTTTTTGGACCGATACAATTTCACTGCCTTACATTAGGTATCTT
GCGCTATAAGCGCGTCCTTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSRISYRRLDGILLLDKPAGISSNSALQVARRLLRAKKGGHTGSLDPLATGLLPLCFGEATKIAGLLLDSTKAYDADVLL
GTTTTTDDAEGAVLLTRPVPTLDAEMVDALIPQLIGRIRQRAPIYSALKQGGEPLYAKARRGEVVNAPVREVEVHAIELL
FLATPRLQLRVTCGSGTYIRSLVRDLGEILGCGAHINALRRRWVAPFQIPKMLTLDALEQALSAGRDPDSLLLPLVEGLA
GFPALELDAQRLACFRLGQRLRDHSFQTGRVAVFSRDGIPAGLGEVDSEGLLVPQRLFNL

Sequences:

>Translated_300_residues
MSRISYRRLDGILLLDKPAGISSNSALQVARRLLRAKKGGHTGSLDPLATGLLPLCFGEATKIAGLLLDSTKAYDADVLL
GTTTTTDDAEGAVLLTRPVPTLDAEMVDALIPQLIGRIRQRAPIYSALKQGGEPLYAKARRGEVVNAPVREVEVHAIELL
FLATPRLQLRVTCGSGTYIRSLVRDLGEILGCGAHINALRRRWVAPFQIPKMLTLDALEQALSAGRDPDSLLLPLVEGLA
GFPALELDAQRLACFRLGQRLRDHSFQTGRVAVFSRDGIPAGLGEVDSEGLLVPQRLFNL
>Mature_299_residues
SRISYRRLDGILLLDKPAGISSNSALQVARRLLRAKKGGHTGSLDPLATGLLPLCFGEATKIAGLLLDSTKAYDADVLLG
TTTTTDDAEGAVLLTRPVPTLDAEMVDALIPQLIGRIRQRAPIYSALKQGGEPLYAKARRGEVVNAPVREVEVHAIELLF
LATPRLQLRVTCGSGTYIRSLVRDLGEILGCGAHINALRRRWVAPFQIPKMLTLDALEQALSAGRDPDSLLLPLVEGLAG
FPALELDAQRLACFRLGQRLRDHSFQTGRVAVFSRDGIPAGLGEVDSEGLLVPQRLFNL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=237, Percent_Identity=34.1772151898734, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI2367200, Length=303, Percent_Identity=41.9141914191419, Blast_Score=208, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6324037, Length=173, Percent_Identity=30.635838150289, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=239, Percent_Identity=28.8702928870293, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32396; Mature: 32265

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRISYRRLDGILLLDKPAGISSNSALQVARRLLRAKKGGHTGSLDPLATGLLPLCFGEA
CCCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCH
TKIAGLLLDSTKAYDADVLLGTTTTTDDAEGAVLLTRPVPTLDAEMVDALIPQLIGRIRQ
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
RAPIYSALKQGGEPLYAKARRGEVVNAPVREVEVHAIELLFLATPRLQLRVTCGSGTYIR
CCHHHHHHHCCCCCEEHHCCCCCCCCCCHHHHHHHHHEEEEEECCCEEEEEEECCCHHHH
SLVRDLGEILGCGAHINALRRRWVAPFQIPKMLTLDALEQALSAGRDPDSLLLPLVEGLA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GFPALELDAQRLACFRLGQRLRDHSFQTGRVAVFSRDGIPAGLGEVDSEGLLVPQRLFNL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
SRISYRRLDGILLLDKPAGISSNSALQVARRLLRAKKGGHTGSLDPLATGLLPLCFGEA
CCCCHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCH
TKIAGLLLDSTKAYDADVLLGTTTTTDDAEGAVLLTRPVPTLDAEMVDALIPQLIGRIRQ
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
RAPIYSALKQGGEPLYAKARRGEVVNAPVREVEVHAIELLFLATPRLQLRVTCGSGTYIR
CCHHHHHHHCCCCCEEHHCCCCCCCCCCHHHHHHHHHEEEEEECCCEEEEEEECCCHHHH
SLVRDLGEILGCGAHINALRRRWVAPFQIPKMLTLDALEQALSAGRDPDSLLLPLVEGLA
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
GFPALELDAQRLACFRLGQRLRDHSFQTGRVAVFSRDGIPAGLGEVDSEGLLVPQRLFNL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]