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The map label for this gene is tgt

Identifier: 182680738

GI number: 182680738

Start: 229999

End: 231132

Strand: Direct

Name: tgt

Synonym: XfasM23_0167

Alternate gene names: 182680738

Gene position: 229999-231132 (Clockwise)

Preceding gene: 182680737

Following gene: 182680739

Centisome position: 9.07

GC content: 53.88

Gene sequence:

>1134_bases
ATGTCTCGATTGCAGTTTCAGCTTCAGGCGACCGATGGTGCTGCCCGTCGTGGTCAATTGAGTTTTCCATGCGGTACCGT
GCAGACGCCAACCTTCATGCCGGTCGGCACGTATGGTGCTGTGAAGGGGGTGTTGCCAGGGCAGCTTTGTGATTTGGGTG
CGGAAATCATCCTTGGTAACACGTTCCACTTGTTTTTGCGCCCAGGTCTGGAGGTGATTGCTGATCATGGCGGTCTGCAT
GGCTTCATGCGCTGGAATGGGCCGATCTTGACGGATTCTGGTGGCTTTCAAGTATTTTCGTTGGCGCACCGGCGCAAGAT
CAGTGAGCAAGGTGTGACGTTTGCAGCACCGACAGATGGTGCTCAGGTGTTTCTTGGACCTGAGGAAAGCATGAAAATCC
AGAAAGTGCTGAATTCGGATGTCGTAATGATTTTCGATGAATGCACGCCTTATCCGGCCACTGAAGATGTGGCGCGTGAC
TCAATGGAGTTGAGTCTGCGGTGGGCGCAGCGTTCACGTGATGCCCATGATGCCTTGGACAATGACGCGGCTCTGTTTGG
GATTATTCAAGGCGGTGTGCATCCTGATCTGCGCGGTCGGTCGTTGGACGGTTTGCAGGCGATCGGCTTTGATGGCTACG
GGATTGGTGGTTTGGCCGTCGGTGAGTCGGAATCTGAGCGCAATGTGATTCTTGAGTACCTGCATCCACGTCTGCCGACG
GACCGACCGCGCTATCTAATGGGGGTGGGTCGACCGGAGGATCTGGTTGAGAGTGTGGCCCGTGGCGTGGATATGTTCGA
TTGTGTGATGCCCACCCGTCATGCGCGTAATGGGCAGTATTTTACTGGATTCGGTACGGTTAAGATCCGTAATGCTTGTT
ATGCGAGGGATGTGGATCCCATTGAGCAGGGCTGTGGTTGTCCGGCTTGTGTTGGCGGCTATACTCGTGCTTACCTCCGC
CATTTGGACCGTTGTAACGAGATGTTGGCATCAATGCTTGGTTCCTTACATAACCTTTGGTACTACGAAACATTGATGGC
TAATATGCGTGCGGCGATTACAGCGGGGACTTTTTTTGCATTCCGGCGTTCTTTCTATCTGGCGCGTGGTCTTGATCCTC
CGCCTCTGCCTTAG

Upstream 100 bases:

>100_bases
TGCTGTAGCAGGATCTTGATTCAGTGATGGCTGTGTTAAGGGTGGGCGTACTGTTTGTAATCCGGGACAATGGAAGGCCG
TTATCGTGTGTGTGTGCCGT

Downstream 100 bases:

>100_bases
GTGGCTGGTTGCGCCGGGTGAGCCAAATGAGATGTCGGCTCTCATGGCATAATTGTCGGCTTACTACCGCTTCGGCGGAT
GCATAAAGTTGGGCATGTCT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSRLQFQLQATDGAARRGQLSFPCGTVQTPTFMPVGTYGAVKGVLPGQLCDLGAEIILGNTFHLFLRPGLEVIADHGGLH
GFMRWNGPILTDSGGFQVFSLAHRRKISEQGVTFAAPTDGAQVFLGPEESMKIQKVLNSDVVMIFDECTPYPATEDVARD
SMELSLRWAQRSRDAHDALDNDAALFGIIQGGVHPDLRGRSLDGLQAIGFDGYGIGGLAVGESESERNVILEYLHPRLPT
DRPRYLMGVGRPEDLVESVARGVDMFDCVMPTRHARNGQYFTGFGTVKIRNACYARDVDPIEQGCGCPACVGGYTRAYLR
HLDRCNEMLASMLGSLHNLWYYETLMANMRAAITAGTFFAFRRSFYLARGLDPPPLP

Sequences:

>Translated_377_residues
MSRLQFQLQATDGAARRGQLSFPCGTVQTPTFMPVGTYGAVKGVLPGQLCDLGAEIILGNTFHLFLRPGLEVIADHGGLH
GFMRWNGPILTDSGGFQVFSLAHRRKISEQGVTFAAPTDGAQVFLGPEESMKIQKVLNSDVVMIFDECTPYPATEDVARD
SMELSLRWAQRSRDAHDALDNDAALFGIIQGGVHPDLRGRSLDGLQAIGFDGYGIGGLAVGESESERNVILEYLHPRLPT
DRPRYLMGVGRPEDLVESVARGVDMFDCVMPTRHARNGQYFTGFGTVKIRNACYARDVDPIEQGCGCPACVGGYTRAYLR
HLDRCNEMLASMLGSLHNLWYYETLMANMRAAITAGTFFAFRRSFYLARGLDPPPLP
>Mature_376_residues
SRLQFQLQATDGAARRGQLSFPCGTVQTPTFMPVGTYGAVKGVLPGQLCDLGAEIILGNTFHLFLRPGLEVIADHGGLHG
FMRWNGPILTDSGGFQVFSLAHRRKISEQGVTFAAPTDGAQVFLGPEESMKIQKVLNSDVVMIFDECTPYPATEDVARDS
MELSLRWAQRSRDAHDALDNDAALFGIIQGGVHPDLRGRSLDGLQAIGFDGYGIGGLAVGESESERNVILEYLHPRLPTD
RPRYLMGVGRPEDLVESVARGVDMFDCVMPTRHARNGQYFTGFGTVKIRNACYARDVDPIEQGCGCPACVGGYTRAYLRH
LDRCNEMLASMLGSLHNLWYYETLMANMRAAITAGTFFAFRRSFYLARGLDPPPLP

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=363, Percent_Identity=43.5261707988981, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=60.5898123324397, Blast_Score=462, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=39.6694214876033, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI25146502, Length=139, Percent_Identity=28.7769784172662, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=42.8184281842818, Blast_Score=293, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41374; Mature: 41243

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLQFQLQATDGAARRGQLSFPCGTVQTPTFMPVGTYGAVKGVLPGQLCDLGAEIILGN
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCHHHHCCCEEEECC
TFHLFLRPGLEVIADHGGLHGFMRWNGPILTDSGGFQVFSLAHRRKISEQGVTFAAPTDG
EEEEEECCCHHHHHCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCEEECCCCC
AQVFLGPEESMKIQKVLNSDVVMIFDECTPYPATEDVARDSMELSLRWAQRSRDAHDALD
CEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCHHHEEHHHHHCCHHHHHCC
NDAALFGIIQGGVHPDLRGRSLDGLQAIGFDGYGIGGLAVGESESERNVILEYLHPRLPT
CCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCHHHHHHHHCCCCCC
DRPRYLMGVGRPEDLVESVARGVDMFDCVMPTRHARNGQYFTGFGTVKIRNACYARDVDP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECEEEECCCHHCCCCCH
IEQGCGCPACVGGYTRAYLRHLDRCNEMLASMLGSLHNLWYYETLMANMRAAITAGTFFA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRRSFYLARGLDPPPLP
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRLQFQLQATDGAARRGQLSFPCGTVQTPTFMPVGTYGAVKGVLPGQLCDLGAEIILGN
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCHHHHCCCEEEECC
TFHLFLRPGLEVIADHGGLHGFMRWNGPILTDSGGFQVFSLAHRRKISEQGVTFAAPTDG
EEEEEECCCHHHHHCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCEEECCCCC
AQVFLGPEESMKIQKVLNSDVVMIFDECTPYPATEDVARDSMELSLRWAQRSRDAHDALD
CEEEECCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCHHHEEHHHHHCCHHHHHCC
NDAALFGIIQGGVHPDLRGRSLDGLQAIGFDGYGIGGLAVGESESERNVILEYLHPRLPT
CCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCEEEECCCCCCHHHHHHHHCCCCCC
DRPRYLMGVGRPEDLVESVARGVDMFDCVMPTRHARNGQYFTGFGTVKIRNACYARDVDP
CCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCEEEEECEEEECCCHHCCCCCH
IEQGCGCPACVGGYTRAYLRHLDRCNEMLASMLGSLHNLWYYETLMANMRAAITAGTFFA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRRSFYLARGLDPPPLP
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]