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The map label for this gene is dut

Identifier: 182680683

GI number: 182680683

Start: 151445

End: 151912

Strand: Direct

Name: dut

Synonym: XfasM23_0112

Alternate gene names: 182680683

Gene position: 151445-151912 (Clockwise)

Preceding gene: 182680682

Following gene: 182680684

Centisome position: 5.97

GC content: 53.63

Gene sequence:

>468_bases
ATGAGTGCTGCTGTAAAACCACTGCAGATCAAGATTCTGGATCCTCGTCTCGGTACCGTTTGGCCGCTGCCGACCTATGC
GACGGAAGCAAGTGCTGGGCTTGATTTACGGGCGGCACTGGATGCACCAATGACTCTGGTTCCTGGGGATGCGGAGTTAC
TTTCTACGGGTATAGCGATTCACTTAGTTGACCCGAGCTTGTGTGCAGTGGTATTGCCTCGTTCCGGTTTGGGACATCGT
CATGGGATTGTGCTCGGCAATGGCACGGGTTTGATTGATTCAGACTATCAGGGGCCGTTGTTGGTGAGTGTTTGGAACCG
TGGGCGCGAGGCTTTTACTATCGAGCCTGGCGACCGTATCGCCCAGTTGGTTGTGTTACCAATTGTGCGTGTAGTGCTTC
AGGTAGTGGATACTTTTGTTGAAAGTGGGCGTGGAGCGGGTGGTTTTGGTCACACTGGCGTGCGTTGA

Upstream 100 bases:

>100_bases
TCACTGCGTATTGGCAGGGTGGCGAACGCGTTTTCGCTAGTTGCAGTAAGGTGGAGTTGGCTGAGCGCCTGCTGGCACTG
ATTGCTGAAAGGATGCAGAC

Downstream 100 bases:

>100_bases
CGGAGGAAGACAGATGAGCGTGACAATTGGAAACGAGTTGATTTTGCCGATGAAACGACGCCGGATTGCATTTCTTTTGG
CGATGTTGTGTTTGTTGCTG

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MSAAVKPLQIKILDPRLGTVWPLPTYATEASAGLDLRAALDAPMTLVPGDAELLSTGIAIHLVDPSLCAVVLPRSGLGHR
HGIVLGNGTGLIDSDYQGPLLVSVWNRGREAFTIEPGDRIAQLVVLPIVRVVLQVVDTFVESGRGAGGFGHTGVR

Sequences:

>Translated_155_residues
MSAAVKPLQIKILDPRLGTVWPLPTYATEASAGLDLRAALDAPMTLVPGDAELLSTGIAIHLVDPSLCAVVLPRSGLGHR
HGIVLGNGTGLIDSDYQGPLLVSVWNRGREAFTIEPGDRIAQLVVLPIVRVVLQVVDTFVESGRGAGGFGHTGVR
>Mature_154_residues
SAAVKPLQIKILDPRLGTVWPLPTYATEASAGLDLRAALDAPMTLVPGDAELLSTGIAIHLVDPSLCAVVLPRSGLGHRH
GIVLGNGTGLIDSDYQGPLLVSVWNRGREAFTIEPGDRIAQLVVLPIVRVVLQVVDTFVESGRGAGGFGHTGVR

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI4503423, Length=152, Percent_Identity=38.8157894736842, Blast_Score=88, Evalue=4e-18,
Organism=Homo sapiens, GI70906444, Length=139, Percent_Identity=40.2877697841727, Blast_Score=87, Evalue=4e-18,
Organism=Homo sapiens, GI70906441, Length=152, Percent_Identity=38.8157894736842, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=61.0738255033557, Blast_Score=200, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI71988561, Length=131, Percent_Identity=41.9847328244275, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319729, Length=132, Percent_Identity=38.6363636363636, Blast_Score=79, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921126, Length=133, Percent_Identity=35.3383458646617, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583610, Length=133, Percent_Identity=35.3383458646617, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_XYLF2 (B2I6M7)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828843.1
- ProteinModelPortal:   B2I6M7
- SMR:   B2I6M7
- GeneID:   6203455
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0112
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- ProtClustDB:   PRK00601
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16246; Mature: 16115

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: BINDING 87-87

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAVKPLQIKILDPRLGTVWPLPTYATEASAGLDLRAALDAPMTLVPGDAELLSTGIAI
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCHHHHHCCEEE
HLVDPSLCAVVLPRSGLGHRHGIVLGNGTGLIDSDYQGPLLVSVWNRGREAFTIEPGDRI
EEECCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHH
AQLVVLPIVRVVLQVVDTFVESGRGAGGFGHTGVR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAAVKPLQIKILDPRLGTVWPLPTYATEASAGLDLRAALDAPMTLVPGDAELLSTGIAI
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCHHHHHCCEEE
HLVDPSLCAVVLPRSGLGHRHGIVLGNGTGLIDSDYQGPLLVSVWNRGREAFTIEPGDRI
EEECCCEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHH
AQLVVLPIVRVVLQVVDTFVESGRGAGGFGHTGVR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA