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The map label for this gene is speE

Identifier: 182680676

GI number: 182680676

Start: 142964

End: 143821

Strand: Reverse

Name: speE

Synonym: XfasM23_0105

Alternate gene names: 182680676

Gene position: 143821-142964 (Counterclockwise)

Preceding gene: 182680678

Following gene: 182680672

Centisome position: 5.67

GC content: 51.4

Gene sequence:

>858_bases
ATGACCACCAACGACACCTGGTTCACCGAACATTTCCAAACAACCGGCTCAGCGATCGGCTTCCGCGTCACTGGCAAACT
AGATGAAGTGCAATCACCATTCCAGAAAATCGAAATCTACAACAGCACCGACTGGGGCAAACTCATGGTGATCGACGGCG
CATTAATGCTCACCAGTAGAGACAATTTCCTCTATCACGAAATGATCAGCCATCCAGCACTATTCACCCACACCGCCCCA
AAGTGCGTGGTGATTATCGGCGGCGGCGACTGCGGGACACTGCGCGAAGTCCTCAAACACCCAGACATAGAACAAGTCAC
CCAATGCGATATTGATGAACAAGTCACACGCATGGCGGAAAAACATTTCCCTGAACTATGCACCTCCAACAACGACCCAC
GCGCCACACTGCTGTTCAGCGACGGCGTGGCTTATATGGCCGATTGCCCAACCAACAGCGTGGATGTGATTATCGTCGAC
TCAACCGACCCTGTCGGACCAGCCAAAGGCTTATTCAACAGAACCTTCTACGAAAGCTGTTTCCGCGCCCTAAAAAATGA
TGGATTACTCATACAACAATCAGAATCACCATTGGCACTACTAGAACTCATCAAAGAAATGCGCCATGAAATGAGCAAAG
CCGGCTTCAAAGCATTCAAGACACTACCGTTTCCACAACCTTGCTATCCCACCGGCTGGTGGAGCGTCACCCTATCCAGC
AAACAACCCAACGCGAACTTTGCATTTCGCCAAACCGACGCCCAAACCAAACCCTTCGACACCCTGTACTACAACGCACA
CCTGCATCATGGTGTACTCGTCCCGCCCCCCTTCATCGCACACGCATTAGGCGAGTAA

Upstream 100 bases:

>100_bases
ACTACATCCCATCCCAAACAGATCACGGCCACGTTGACACCTTCCCGCAGCACCTTGCTCCACACCGGAACAGGCCACCT
ACCAGCCAAAGATTCTCCAC

Downstream 100 bases:

>100_bases
CCCCAAAACCAACCGCATTTGAATGCCTGCTGTTGCCAGCCATCAGCGGGCAATCCCATGCCCCCTACGCACAATAGCGT
GCTCACACTAACACCGGCAC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTTNDTWFTEHFQTTGSAIGFRVTGKLDEVQSPFQKIEIYNSTDWGKLMVIDGALMLTSRDNFLYHEMISHPALFTHTAP
KCVVIIGGGDCGTLREVLKHPDIEQVTQCDIDEQVTRMAEKHFPELCTSNNDPRATLLFSDGVAYMADCPTNSVDVIIVD
STDPVGPAKGLFNRTFYESCFRALKNDGLLIQQSESPLALLELIKEMRHEMSKAGFKAFKTLPFPQPCYPTGWWSVTLSS
KQPNANFAFRQTDAQTKPFDTLYYNAHLHHGVLVPPPFIAHALGE

Sequences:

>Translated_285_residues
MTTNDTWFTEHFQTTGSAIGFRVTGKLDEVQSPFQKIEIYNSTDWGKLMVIDGALMLTSRDNFLYHEMISHPALFTHTAP
KCVVIIGGGDCGTLREVLKHPDIEQVTQCDIDEQVTRMAEKHFPELCTSNNDPRATLLFSDGVAYMADCPTNSVDVIIVD
STDPVGPAKGLFNRTFYESCFRALKNDGLLIQQSESPLALLELIKEMRHEMSKAGFKAFKTLPFPQPCYPTGWWSVTLSS
KQPNANFAFRQTDAQTKPFDTLYYNAHLHHGVLVPPPFIAHALGE
>Mature_284_residues
TTNDTWFTEHFQTTGSAIGFRVTGKLDEVQSPFQKIEIYNSTDWGKLMVIDGALMLTSRDNFLYHEMISHPALFTHTAPK
CVVIIGGGDCGTLREVLKHPDIEQVTQCDIDEQVTRMAEKHFPELCTSNNDPRATLLFSDGVAYMADCPTNSVDVIIVDS
TDPVGPAKGLFNRTFYESCFRALKNDGLLIQQSESPLALLELIKEMRHEMSKAGFKAFKTLPFPQPCYPTGWWSVTLSSK
QPNANFAFRQTDAQTKPFDTLYYNAHLHHGVLVPPPFIAHALGE

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=286, Percent_Identity=37.4125874125874, Blast_Score=188, Evalue=4e-48,
Organism=Escherichia coli, GI1786312, Length=272, Percent_Identity=30.8823529411765, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI25154106, Length=274, Percent_Identity=37.5912408759124, Blast_Score=162, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI212645616, Length=322, Percent_Identity=32.6086956521739, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325326, Length=287, Percent_Identity=35.5400696864112, Blast_Score=179, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323175, Length=293, Percent_Identity=33.4470989761092, Blast_Score=152, Evalue=8e-38,
Organism=Drosophila melanogaster, GI24645443, Length=282, Percent_Identity=33.3333333333333, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI21355941, Length=213, Percent_Identity=34.2723004694836, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_XYLF2 (B2I6M0)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001828836.1
- ProteinModelPortal:   B2I6M0
- SMR:   B2I6M0
- GeneID:   6203245
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0105
- HOGENOM:   HBG635113
- OMA:   DGHIYNE
- ProtClustDB:   PRK00811
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31922; Mature: 31790

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 35-35 BINDING 90-90 BINDING 110-110 BINDING 160-160 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNDTWFTEHFQTTGSAIGFRVTGKLDEVQSPFQKIEIYNSTDWGKLMVIDGALMLTSR
CCCCCCCHHHHHHCCCCEEEEEEECCHHHHHCCHHHEEEECCCCCCEEEEEECEEEEEEC
DNFLYHEMISHPALFTHTAPKCVVIIGGGDCGTLREVLKHPDIEQVTQCDIDEQVTRMAE
CCHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHH
KHFPELCTSNNDPRATLLFSDGVAYMADCPTNSVDVIIVDSTDPVGPAKGLFNRTFYESC
HHCHHHHCCCCCCEEEEEEECCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
FRALKNDGLLIQQSESPLALLELIKEMRHEMSKAGFKAFKTLPFPQPCYPTGWWSVTLSS
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
KQPNANFAFRQTDAQTKPFDTLYYNAHLHHGVLVPPPFIAHALGE
CCCCCCEEEEECCCCCCCCEEEEEEEEECCCEECCCHHHHHHCCC
>Mature Secondary Structure 
TTNDTWFTEHFQTTGSAIGFRVTGKLDEVQSPFQKIEIYNSTDWGKLMVIDGALMLTSR
CCCCCCHHHHHHCCCCEEEEEEECCHHHHHCCHHHEEEECCCCCCEEEEEECEEEEEEC
DNFLYHEMISHPALFTHTAPKCVVIIGGGDCGTLREVLKHPDIEQVTQCDIDEQVTRMAE
CCHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHCCCHHHHHHCCHHHHHHHHHH
KHFPELCTSNNDPRATLLFSDGVAYMADCPTNSVDVIIVDSTDPVGPAKGLFNRTFYESC
HHCHHHHCCCCCCEEEEEEECCCEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHH
FRALKNDGLLIQQSESPLALLELIKEMRHEMSKAGFKAFKTLPFPQPCYPTGWWSVTLSS
HHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECC
KQPNANFAFRQTDAQTKPFDTLYYNAHLHHGVLVPPPFIAHALGE
CCCCCCEEEEECCCCCCCCEEEEEEEEECCCEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA